WO2005090548A1 - Dispositif de maintien pour incubateur - Google Patents

Dispositif de maintien pour incubateur Download PDF

Info

Publication number
WO2005090548A1
WO2005090548A1 PCT/JP2005/004044 JP2005004044W WO2005090548A1 WO 2005090548 A1 WO2005090548 A1 WO 2005090548A1 JP 2005004044 W JP2005004044 W JP 2005004044W WO 2005090548 A1 WO2005090548 A1 WO 2005090548A1
Authority
WO
WIPO (PCT)
Prior art keywords
incubator
holding member
holding
movable plate
cells
Prior art date
Application number
PCT/JP2005/004044
Other languages
English (en)
Japanese (ja)
Inventor
Keiji Naruse
Norio Ishida
Original Assignee
Nagoya Industrial Science Research Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nagoya Industrial Science Research Institute filed Critical Nagoya Industrial Science Research Institute
Priority to JP2006511160A priority Critical patent/JPWO2005090548A1/ja
Publication of WO2005090548A1 publication Critical patent/WO2005090548A1/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/48—Holding appliances; Racks; Supports
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/14—Incubators; Climatic chambers

Definitions

  • the present invention relates to an improvement in a holding device for holding a culture device, in a culture device for mounting a culture device for culturing a sample cell while generating stress on the sample cell.
  • Patent Documents 13 to 13 disclose a technique in which a bottom membrane of a container on which a sample cell is placed is deformed by suction, thereby generating stress on the sample cell.
  • a sample cell is placed on a 200 ⁇ m-thick translucent and deformable culture membrane 3 coated with fibronectin, and a pair of opposite sides of the culture membrane 3 (Thickness: 400 / zm), side walls 5 and 5 are provided (see Non-Patent Document 1).
  • the reason why the thick side walls 5 are used is to uniformly spread the culture membrane 3 on which the sample cells are placed.
  • an engagement hole 7 is formed in the side wall 5.
  • a pin (not shown) of the extension device 10 is inserted into the engagement hole 7.
  • the extension device 10 includes a fixed plate (first holding member) 11, a movable plate (second holding member) 13, a step motor 15, and a control device 17, as shown in FIG.
  • Pins are formed at predetermined positions on the fixed plate 11 and the movable plate 13, and the engagement holes 7 of the incubator 1 are fitted to the respective pins. Thereby, the incubator 1 is suspended between the fixed plate 11 and the movable plate 13. The distance between the movable plate 13 and the fixed plate 11 changes according to the rotation of the step motor 15. As a result, the culture membrane 3 of the incubator 1 It extends and thereby stresses the sample cells. The rotation of the step motor 15 is controlled by the controller 17.
  • Patent Documents 116 As related to the present invention, see also Patent Documents 116.
  • Patent Document 1 US Pat. No. 4,789,601
  • Patent Document 2 U.S. Pat.No. 4,827,241
  • Patent Document 3 US Pat. No. 4,839,280
  • Patent Document 4 JP 2003-61642 A
  • Patent Document 5 US Patent No. 6107081
  • Patent Document 6 International Publication WO02 / 46365
  • the incubator 1 When the incubator 1 is set in the incubator shown in Fig. 1 above, the entire incubator including the step motor 15 and the controller 17 is put into a clean bench, in which the fixed plate 11 is placed. And the incubator 1 was attached to the movable plate 13. Thereafter, the culture apparatus was moved to an environment suitable for cell culture, and was driven there, thereby applying stress to the cells in the incubator 1.
  • the culturing apparatus including the drive unit such as the step motor 15 becomes large in size and heavy in weight, so that it takes time and effort to move the culturing apparatus.
  • the positional relationship between the fixed plate and the movable plate may be irregularly shifted during the movement of the incubator. If such a deviation occurs, the incubator 1 is deformed, and an unexpected stress is applied to the cells in the incubator 1, which is not preferable.
  • an object of the present invention is to make it easier to handle an incubator in a culture apparatus for applying stress to cells in the incubator.
  • the present invention has been made to solve at least one of the above problems. That is, the first holding member and the second holding member on which the incubator is suspended, wherein the first holding member and the second holding member are detachable from the culture device main body.
  • An incubator holding device comprising:
  • both the first holding member and the second holding member are detachable from the main body of the culture apparatus. Therefore, the first holding member and the second holding member can be detached from the culture device main body, put into the clean bench, and the incubator can be set there. Then, both holding members on which the incubator is set are returned to the main body of the incubator.
  • the number of elements to be moved is reduced as much as possible, so that the handling is facilitated. That is, there is no time and effort for moving.
  • first holding member and the second holding member are connected by the connecting member, it is possible to prevent an irregular displacement in the positional relationship between the two. Therefore, it is possible to prevent an unexpected stress from being applied to the cells in the incubator.
  • a lid covering at least the opening of the incubator is provided.
  • FIG. 1 shows a conventional incubator and its extension device.
  • FIG. 2 is a perspective view of an incubator according to an embodiment of the present invention.
  • FIG. 3 is a perspective view showing a configuration of a holding device of the embodiment.
  • FIG. 4 is a plan view of the same.
  • FIG. 5 is a cross-sectional view illustrating a configuration of a holding device according to another embodiment.
  • FIG. 6 is a plan view showing a schematic configuration of a culture device.
  • FIG. 7 is a longitudinal sectional view showing a schematic configuration of the culture apparatus.
  • FIG. 8 is a front view of the culture device.
  • the culture apparatus main body is provided with a first holding member seat and a second holding member seat for detachably fixing the first holding member and the second holding member. Further, a mechanism for changing the relative position of each holding member seat is provided.
  • the first holding member seat is fixed and the second holding member seat is movable, but both the holding member seats may be movable.
  • the culture device main body, with the first holding member and the second holding member mounted thereon, is suitable for cell culture when controlling the atmosphere of both holding members and applying stress thereto. Can be In order to create the atmosphere, the culture apparatus main body is provided with a temperature controller and a humidity controller.
  • both holding members are provided with an atmosphere filled with CO gas, cells can be removed in the incubator.
  • the first holding member and the second holding member are formed with engaging portions for engaging with the incubator.
  • a pin is erected from each holding member as the engaging portion.
  • the incubator is suspended between the first holding member and the second holding member by fitting the engagement hole of the incubator into this pin.
  • the shape and structure of the engaging portion are not particularly limited as long as a sufficient force is applied to stably deform the incubator.
  • the first holding member and the second holding member are detachably fixed to a holding member seat of the apparatus main body. This facilitates the handling of both holding members.
  • the holes of the holding members are fitted into the pins provided upright from the holding member seats, and the holding members and the holding member seats are detachably fixed by hooking.
  • the manner of fixing the both is not limited to the structure of the embodiment, but may be clipping, screwing or other well-known structures.
  • the shape, structure and material of the holding member are not particularly limited as long as they can stably hold the incubator. In the embodiment, it is also possible to use a plate-shaped force rod-shaped one or a block-shaped one. The number of incubators set on the holding member can also be arbitrarily selected. [0014] (Connector)
  • the first holding member and the second holding member are connected by a connector.
  • This connecting member prevents at least both holding members from coming apart. This makes handling of the device easier.
  • the coupling tool regulates a variation in the relative distance between the two. Thereby, it is possible to prevent the gap between the two holding members from being irregularly displaced during the movement and applying a stress to the cells in the incubator.
  • a rod is swelled from the first holding member, and the second holding member is slidably fitted to the rod in this embodiment.
  • the configuration of the connecting member is not limited to this, and at least one of the two holding members may be slidably attached to the rail as the connecting member.
  • the connecting member may be a member that fixes and connects both holding members. For example, both holding members are clipped with a clip as a connecting tool. Further, an engagement hole can be formed in both holding members, and a connecting rod can be bridged between the engagement holes to fix and connect the both holding members.
  • a lid is used to cover the incubator set between the first holding member and the second holding member. Accordingly, it is possible to prevent dust and the like from entering the incubator when the apparatus is moved (that is, when the incubator is moved).
  • the lid has substantially the same length as both holding members. Thereby, it becomes possible to cover all the incubators set on both holding members.
  • the shape and material of the lid can be arbitrarily selected according to the target incubator.
  • a flat plate can be used as the lid.
  • the incubator is capable of culturing cells, and is capable of deforming and culturing itself to exert a desired stress on the cells.
  • a rectangular box type shown in FIG. 2 can be used.
  • Kagaruka incubators are made of deformable materials. This is because stress is applied to the sample cells indirectly by extending the incubator. A material that does not chemically interfere with the sample cells, such as silicone elastomer, is used as a material for forming the incubator.
  • the bottom film 23 has a rectangular shape in a plan view and has the same thickness as a whole so as to extend uniformly.
  • the bottom membrane 23 is preferably formed of a light transmitting material so that the sample cells can be observed with an optical microscope.
  • the side wall is erected from the entire periphery of the bottom film.
  • the side walls are made thick to provide mechanical rigidity. Thereby, the bottom film can be prevented from being irregularly deformed.
  • the bottom film and the side wall are integrated from the viewpoint of reduction in the number of parts and, consequently, the production cost, but this does not limit the separate formation of the bottom film and the side wall.
  • Engaging holes 27 are provided in side walls 25 provided on a pair of opposing edges of the rectangular bottom film. What is necessary is just to engage this engaging part with the 1st holding member and the 2nd holding member. Thus, the change in the position of the two members whose relative positions can be controlled causes the incubator to be deformed.
  • the engaging hole 27 is formed on an extension of the peripheral edge 26 in the extension direction of the rectangular bottom membrane.
  • the type and method of obtaining the sample cells used in the incubator 21 of this embodiment are not particularly limited.
  • vascular endothelial cells such as human 'monkey pig' ⁇ ⁇ shi 'rat' mouse ' ⁇ heron
  • smooth muscle cells cardiomyocytes, skeletal muscle cells, fibroblasts, osteoblasts, chondrocytes, osteoclasts, nerves Cells and the like can be used.
  • FIG. 3 is a perspective view showing the holding device 30 of this embodiment.
  • FIG. 4 shows a plan view of the same.
  • the holding device 30 according to the embodiment is roughly composed of a fixed plate 31 as a first holding member, a movable plate 41 as a second holding member, a coupling tool 50, and a lid 60.
  • a through hole 33 is formed in one long edge of the fixing plate 31.
  • a pin 35 is provided upright on the other long edge.
  • a through hole 43 is also formed on one long edge of the movable plate 41, and a pin 45 is provided upright on the other long edge.
  • the incubator 21 is stably suspended between the fixed plate 31 and the movable plate 41 by forcibly fitting the engagement holes 27 of the incubator 27 with the pins 35 and 45 of the plates 31 and 41. Is done.
  • the incubator 27 can be deformed in relation to the change in the distance between the fixed plate 31 and the movable plate 41. Therefore, a desired stress can be applied to the cells in the incubator 27 by controlling the interval between the two plates.
  • Two connecting rods 51 as connecting tools are fixed to the fixing plate 31.
  • a movable plate 41 is slidably fitted to the connecting rod 51. Due to the connecting rod 51, a change in the position of the movable plate 41 with respect to the fixed plate 31 is restricted to only one side (in the axial direction of the connecting rod 51). Therefore, irregular movement of the fixed plate 31 and the movable plate 41 in a state where the incubator 21 is set as shown in FIG. 3 can be prevented.
  • holes 37 and 47 are formed in the side surfaces of the fixed plate 31 and the movable plate 41, and a fixing rod 55 as a fixing tool is fitted into the holes 37 and 47, so that both sides are formed. Plates 31 and 41 can be fixed. Thereby, deformation of the incubator 21 can be completely prevented, and irregular stress can be prevented from being applied to the cells in the incubator 21.
  • Reference numeral 53 in the figure denotes a stopper, which prevents the movable plate 41 from falling off.
  • the lid 60 also covers all of the four incubators 21 set on the plates 31 and 41. Accordingly, even when the incubator 21 is removed from the clean bench, foreign matter such as dust does not enter the incubator 21.
  • the cover 60 is preferably formed of a lightweight material such as resin to facilitate handling during transportation. Further, it is preferable that the incubator 21 is formed of a transparent material so that the state thereof can be observed. In this embodiment, the lid 61 is formed of a transparent acrylic resin.
  • the strong holding device 30 is set on the device main body 71 of the culture device 70 as shown in FIGS. 6 and 7.
  • the culture device 70 includes the holding device 30 and the device body 71 described above.
  • the apparatus main body 71 is provided with four fixed plate seats 75 for detachably fixing the fixed plate 31 and four movable plate seats 77 for detachably fixing the movable plate 41.
  • the holding device 30 is set only on the upper two fixed plate seats 75 and the movable plate seats 77!
  • the movable plate seat 77 is moved by a step motor 79.
  • Reference numeral 81 denotes a rail for moving the movable plate 77
  • reference numeral 83 denotes a control device for controlling the rotation of the step motor 79.
  • the rotational motion of the shaft 85 is converted by the step motor 79 into the axial movement of the rod 89 by the converter 87.
  • the movable plate seat 77 is connected to the rod 89. Therefore, with the rotation of the step motor 79, the movable plate seat 77 moves to the positions shown in FIGS. , Then move left and right.
  • the control device 83 and the step motor 79 constitute a distance control means between the fixed plate 31 and the movable plate 41.
  • Positioning pins 91 and 92 are provided upright on the fixed plate seat 75 and the movable plate seat 77, and are fitted into the through holes 33 of the fixed plate 31 and the through holes 43 of the movable plate 41, respectively.
  • Hooks 93 and 94 are rotatably attached to the fixed plate seat 75 and the movable plate seat 77, respectively.
  • the hook 93 rotates so as to press the upper surface of the fixed plate 31 placed on the fixed plate seat 75.
  • the hook 94 rotates so as to press the upper surface of the movable plate 41 placed on the movable plate seat 77.
  • Locating pins 91, 92 and hooks 93, 94 [Hold each plate 31, 41]
  • Each plate seat 75, 77 [Holds each stably.
  • the hooks 93, 94 By rotating the hooks 93, 94 by 90 degrees, the plates 31, 41 can be detached from the plate seats 75, 77. That is, the hooks 93 and 94 constitute detachable means, whereby the fixed plate 31 and the movable plate 41 are detachably attached to the culture apparatus main body 71.
  • the fixed plate and the movable plate are detachably fixed to each plate seat.
  • the method is not limited to the above.
  • a clip separate from both may be prepared, and the plate and the plate seat may be fixed with the clip.
  • the chamber portion 100 in which the holding device 30 is set becomes airtight by closing the lid 101.
  • the chamber part 100 is provided with a temperature controller 104 and a humidity controller 105. Furthermore, it can be controlled to 5% carbon dioxide and 100% humidity.
  • FIG. 8 shows a front view of the culture device 70.
  • the operator inputs extension conditions of the incubator 21 (that is, stress conditions applied to cells) via the input device 110. Also, input the environmental conditions (temperature, etc.) of the chamber part 100. Based on the input, the control device 83 controls the rotation of the step motor 79 and controls the temperature controller 104 and the humidity controller 105.
  • reference numeral 111 denotes a display.
  • the holding device 31 can be attached to and detached from the device main body 71.
  • the incubator 21 can be moved independently of the apparatus main body 71 with the incubator 21 set in the holding apparatus 31. Therefore, handling of the incubator 21 after setting on the fixed plate 31 and the movable plate 41 becomes easy. Since the positional change between the fixed plate 31 and the movable plate 41 is regulated by the connecting rod 51, irregular deformation of the incubator 21 is prevented. Thereby, unnecessary stress is not applied to the cells in the incubator 21 during the movement.
  • the lid 61 it is possible to prevent foreign matter from entering the cells in the incubator 21 during movement.
  • the culturing apparatus 70 of this embodiment has excellent operability.

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

[PROBLEMES] Faciliter la manipulation d’un incubateur d’un dispositif à incubateur dans lequel des cellules dans l’incubateur sont soumises à des efforts. [MOYENS DE RESOLUTION DES PROBLEMES] Dispositif de maintien pour un incubateur, comprenant un premier élément de maintien et un deuxième élément de maintien sur lesquels est placé un incubateur, et susceptible d’être attaché et détaché d’un corps de dispositif à incubateur et comportant un raccord pour raccorder le premier élément de maintien et le deuxième élément de maintien.
PCT/JP2005/004044 2004-03-19 2005-03-09 Dispositif de maintien pour incubateur WO2005090548A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2006511160A JPWO2005090548A1 (ja) 2004-03-19 2005-03-09 培養器用保持装置

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2004-079932 2004-03-19
JP2004079932 2004-03-19

Publications (1)

Publication Number Publication Date
WO2005090548A1 true WO2005090548A1 (fr) 2005-09-29

Family

ID=34993693

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2005/004044 WO2005090548A1 (fr) 2004-03-19 2005-03-09 Dispositif de maintien pour incubateur

Country Status (2)

Country Link
JP (1) JPWO2005090548A1 (fr)
WO (1) WO2005090548A1 (fr)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008123508A1 (fr) * 2007-03-30 2008-10-16 Strex Incorporation Procédé d'incubation d'œuf fertilisé et appareil d'incubation d'œuf fertilisé
WO2009014246A1 (fr) * 2007-07-26 2009-01-29 Strex Incorporation Appareil et procédé pour la culture d'une cellule d'œuf
JP2009159925A (ja) * 2008-01-10 2009-07-23 Yamaguchi Univ 細胞への伸展刺激負荷デバイス
JP2013255494A (ja) * 2012-06-13 2013-12-26 sheng-nan Chang 生体力学的負荷測定用容器

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH10155475A (ja) * 1996-12-02 1998-06-16 Toru Takemasa シリコンベルトを使った培養細胞用伸縮刺激負荷装置
US6107081A (en) * 1999-02-05 2000-08-22 The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration Uni-directional cell stretching device

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4940853A (en) * 1988-07-22 1990-07-10 Vandenburgh Herman H Method for growing tissue specimens in vitro

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH10155475A (ja) * 1996-12-02 1998-06-16 Toru Takemasa シリコンベルトを使った培養細胞用伸縮刺激負荷装置
US6107081A (en) * 1999-02-05 2000-08-22 The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration Uni-directional cell stretching device

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CLARK C.B. ET AL: "Uniaxial strain system to investigate strain rate regulation in vitro.", REV SCI INSTRUM., vol. 72, no. 5, 2001, pages 2415 - 2422, XP012039143 *
NAKAGAKI M. ET AL: "Saiboyo Nijiku Sohen Incho Kiko no Kaihatsu to kore o Mochiita Kekkan Naihi Saibo no Rikigaku Oto no Kaiseki.", BIO FRONTIER KOENKAI KOEN RONBUNSHU., vol. 14, 2003, pages 7 - 8, XP002997614 *
SOKABE M. AND NARUSE K. ET AL: "BAiyo Saibo eno Kakushu Shinten Shigekiho sono Riten to Ketten.", THE TISSUE CULTURE., vol. 22, no. 10, 1996, pages 413 - 417, XP002997615 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008123508A1 (fr) * 2007-03-30 2008-10-16 Strex Incorporation Procédé d'incubation d'œuf fertilisé et appareil d'incubation d'œuf fertilisé
JP2008271964A (ja) * 2007-03-30 2008-11-13 Strex Inc 受精卵の培養方法及び受精卵の培養装置
WO2009014246A1 (fr) * 2007-07-26 2009-01-29 Strex Incorporation Appareil et procédé pour la culture d'une cellule d'œuf
JP2009027973A (ja) * 2007-07-26 2009-02-12 Okayama Univ 卵細胞の培養装置及びその方法
JP2009159925A (ja) * 2008-01-10 2009-07-23 Yamaguchi Univ 細胞への伸展刺激負荷デバイス
JP2013255494A (ja) * 2012-06-13 2013-12-26 sheng-nan Chang 生体力学的負荷測定用容器

Also Published As

Publication number Publication date
JPWO2005090548A1 (ja) 2008-01-31

Similar Documents

Publication Publication Date Title
WO2005087913A1 (fr) Dispositif de culture
US8828337B2 (en) Microreactor
US6472202B1 (en) Loading station assembly and method for tissue engineering
EP2717672B1 (fr) Système robotisé pour la manipulation de plantules
WO2005090548A1 (fr) Dispositif de maintien pour incubateur
US7390041B2 (en) Micro-manipulator
EP2598245B1 (fr) Boîte à culture à cavités multiples
JP6987425B2 (ja) 軟骨組織の閉鎖系培養のための方法および装置
JP2007515958A (ja) 培養細胞、細胞培養の方法および機器
CA2737627A1 (fr) Plaque a gouttes suspendues
WO2018149795A1 (fr) Dispositif d'étirement de cellules
US20080166796A1 (en) Device for Cell Culture on Deformable Surfaces
WO2015166625A1 (fr) Instrument de culture cellulaire
WO2009014246A1 (fr) Appareil et procédé pour la culture d'une cellule d'œuf
JP6564866B2 (ja) 貼り付け装置及び貼り付け方法
EP2223179A1 (fr) Procédé de prélèvement d'une pluralité d'échantillons
KR100815858B1 (ko) 생체물질의 분리장치 및 방법
CN104619466B (zh) 铰接装置、具有所述铰接装置的显微操纵器装置及其使用方法
Parness Micro-structured adhesives for climbing applications
Moraes et al. Cell mechanics meets MEMS
Kurata et al. Open-source cell extension system assembled from laser-cut plates
CN213924866U (zh) 一种生物试剂细胞培养箱
CN208701026U (zh) 基因测序芯片固定装置、芯片平台及基因测序仪
Wang et al. Pneumatically Controlled Non-Equibiaxial Cell Stretching Device with Live-cell Imaging
Gokhan et al. Engineered heart tissue: Design considerations and the state of the art

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A1

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BW BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE EG ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NA NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SM SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A1

Designated state(s): GM KE LS MW MZ NA SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LT LU MC NL PL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DPEN Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed from 20040101)
WWE Wipo information: entry into national phase

Ref document number: 2006511160

Country of ref document: JP

NENP Non-entry into the national phase

Ref country code: DE

WWW Wipo information: withdrawn in national office

Country of ref document: DE

122 Ep: pct application non-entry in european phase