WO2005089780A1

WO2005089780A1 - Composition against aids virus and method of selectively inactivating virus-infected cells

Info

Publication number: WO2005089780A1
Application number: PCT/JP2005/004977
Authority: WO
Inventors: Keiji Umeda; Taro Shirakawa; Katsunobu Sakai; Yositaka Nadachi; Takehiko Fujino
Original assignee: Umeda Jimusho Ltd.; Institute Of Rheological Function Of Food Co., Ltd.
Priority date: 2004-03-18
Filing date: 2005-03-18
Publication date: 2005-09-29
Also published as: JPWO2005089780A1

Abstract

An antiviral composition having an effect of selectively inactivating virus-infected cells which comprises, as the active ingredient, iodine ion or a compound forming iodine ion; a functional food containing the antiviral composition as described above and thus having an antiviral function imparted thereto; and an antiviral drug comprising the antiviral composition as described above and having an effect of selectively inactivating virus-infected cells. Thus, it is intended to provide a novel antiviral composition and so on having a function of selectively inactivating AIDS virus-infected cells, viral cancer cells, etc.

Description

Specification

TECHNICAL FIELD The present invention relates to an anti-AIDS virus composition and a method for selectively inactivating virus-infected cells.

The present invention relates to an antiviral composition exhibiting an excellent antiviral action against an AIDS virus or the like, and more particularly, to a virus-infected cell infected with a virus such as an AIDS virus. The present invention relates to an antiviral composition having an inactivating function, a functional food to which the antiviral property is added, and an antiviral drug. INDUSTRIAL APPLICABILITY The present invention has an effect of selectively inactivating and killing virus-infected cells in a living cell system in a technical field such as an antiviral preparation applied to a viral disease caused by infection of various viruses such as an AIDS virus. The present invention provides an antiviral composition and a use thereof. For example, the present invention selectively inactivates AIDS virus-infected cells, viral cancer cells, etc., and prevents, treats, and functional foods for these diseases. It is intended to provide useful new drugs and functional foods.

Background art

[0002] At present, up to 20% of civilians in the African region are infected with AIDS, and rescue of patients in these countries is a very important and urgent issue. . WHO's ability to provide free cocktails, the best therapeutics available at present, is limited in number, with hundreds of deaths occurring daily in the South African region, most of which I'm a child. At present, various promising drugs are being developed.However, due to restrictions such as the Pharmaceutical Affairs Law and the Physician Law, it is prohibited to administer drugs without going through clinical trials. Substances—Even if antiviral substances are developed (Patent Documents 1-2), there is a problem that they cannot be used immediately.

[0003] On the other hand, regarding the situation in Japan, the emergence of various viruses that invade foreign countries, for example, AIDS, spleenitis, SARS, Ebola, etc., has put them at risk of virus infection. It is important to prepare a method to keep the virus level below the onset level. The method must be simple, inexpensive and generally accepted by the general public. For example, food-based methods are considered to be suitable. Also this The same is true of cancer, which is becoming a national disease.

[0004] As described above, the problem of prevention and control of various virus infections, especially the problem of AIDS virus infection, has become an important international issue today with the arrival of Globali Dani. Urgent action is required, especially in developing countries. However, in reality, there are few effective therapeutic agents that can be expected to have a high therapeutic effect at low cost and have a high therapeutic effect. In the art, AIDS virus-infected cells and cancer cells cannot be selectively selected. The development of new therapeutic agents and functional foods that could be activated and killed was urgently required.

[0005] Patent Document 1: Japanese Patent Laid-Open No. 5-294838

Patent Document 2: Japanese Patent Application Laid-Open No. 2002-293803

Disclosure of the invention

Problems to be solved by the invention

[0006] Under such circumstances, the present inventors have considered in view of the above-mentioned prior art that prevention and treatment of viral infectious diseases caused by various viruses such as the AIDS virus, which is low in cost and highly safe, can be performed. As a result of intensive research aimed at developing effective new ヽ functional foods ゃ antiviral preparations, etc., we used the new eodo supply system developed by the present inventors for virus-infected cells using eodo ions. As a result, the present inventors have found that the intended purpose can be achieved, and have conducted further studies to complete the present invention.

[0007] That is, the present invention provides a specific antiviral effect of selectively inactivating virus-infected cells by utilizing an eodo-supply system using oodoion in virus-infected cells such as AIDS virus-infected cells. It is an object of the present invention to provide a novel antiviral composition, a functional food containing the antiviral composition, an antiviral drug, and the like.

Means for solving the problem

[0008] The present invention for solving the above-mentioned problems includes the following technical means.

(1) An antiviral composition having an action to selectively inactivate virus-infected cells, characterized by containing as an active ingredient an ore ion or a compound that produces an ore ion. (2) The antiviral composition according to the above (1), wherein the antiviral composition is a combination of an anode ion and a polyphenol conjugate.

(3) A functional food having an antiviral function, wherein the antiviral composition according to the above (1) or (2) is blended to add an antiviral function.

(4) An antiviral drug having an action of selectively inactivating virus-infected cells, comprising the antiviral composition according to (1) or (2).

(5) Supplying an oluded ion or a compound that produces an iodine ion to a living cell system containing a virus-infected cell to specifically produce an eodo (1Z2I) within the virus-infected cell,

2

The virus (1Z2I) acts to selectively inactivate and kill virus-infected cells.

2

A method for selectively inactivating virus-infected cells, comprising:

(6) Supplying an ode ion or a compound generating an ode ion to a living cell system containing a virus-infected cell to specifically produce an ode (1Z2I) in the virus-infected cell.

2

Selective pseudo (1Z2I) generation in virus-infected cells characterized by

2

Stem.

Next, the present invention will be described in more detail.

The antiviral composition of the present invention is characterized in that it contains, as an active ingredient, an ode ion or a compound that generates an ode ion. Ode (1Z2I) production to supply specifically to cell lines to produce eodo (1Z2I) in virus-infected cells-

22 Provide anti-viral preparations, functional foods with anti-viral function, etc., which are characterized by selectively inactivating and killing virus-infected cells using a derivative system. is there.

[0010] The present invention provides the following novel findings discovered by the present inventors; (1) generation of eaves on the anode side due to electrolysis of ionic ion water; Γ → [· Ι] → 1Ζ2Ι , (2) live cells

2

In the method of carrier-free electrophoresis (electrophoresis with cells suspended without using filter paper or gel), the distribution center of normal cells is neutral ± 0, and the distribution center of virus-infected cells is on the minus side. (3) ΙΗΝ (infectious hematopoietic necrosis virus that infects salmon) infected cells The mode ion has been found to be deelectronized to produce eodes because it has an environment similar to (1) above, and (4) the inodes in infected cells inactivate viruses or cells. It was created based on the fact that it was recognized.

Here, an experimental system that has obtained the above findings will be described. FIG. 1 shows an example of an apparatus used for carrier-free electrophoresis of living cells of a living tissue. In the figure, a is the anode (platinum), b is the connection of the anode terminal, c is the force sword (platinum), _e is the filter paper, f is the filter paper that prevents cell diffusion, g is the buffer, and h is the buffer. After electrophoresis, the electrophoresis tank of the device was replaced with 9 compartments (8 [= node] 4, 83, 82, 81, 3 [= sample], [= cathode] 1, C2, C3, C4) A magnetic ferrite rubber part for mounting means for dividing into two parts, i is a plastic plate used for separating the apparatus into electrophoresis. During the test, the device was controlled at 15 ° C for fish cells or 37 ° C for animal cells. The arrow indicates the position of the cross section.

FIG. 2 shows an electrophoretic diagram of virus-infected RTG-2 cells (cultivable cells collected from germ-line glands of -mouth trout and cultured host cells of the IHN virus or the like). These are electropherograms of virus-infected RTG-2 cells. In (A), electrophoresis was performed at 15 ° C in MEM one day after infection with the IHN virus. Electrophoresis was performed 3 days later. In (C), g-strophantin or sodium azide was added 5 days after the infection and electrophoresis was performed in MEM. In (D), electrophoresis was performed 5 days after the infection. In (E), the cells were electrophoresed at 15 ° C in MEM. In (E), the cells were electrophoresed in MEM one day after infection. In (F), the cells were infected with IPN virus. One day after infection, g-strophantin or sodium azide was added, and the results of electrophoresis with MEM are shown.

[0013] FIG. 3 shows electropherograms of live RTG-2 cells and CHSE-214 cells (culturable cells collected from trout (masunosuke) and host culture cells of IHN virus or the like). These cells show that 107 Zlml of RTG-2 cells or CHSE-214 cells were calored in the center of the electrophoresis apparatus. After standing, in (B), electrophoresis (20 mA, 15 minutes in DC) was performed with EBSS at pH pH 7.0. The results of electrophoresis at 0 are shown. From these results, in carrier-free electrophoresis of living cells, the distribution center of normal cells was neutral and the distribution center of IHN virus-infected cells shifted to the minus side, and the cell membrane surface ( It can be seen that the (cytoplasmic) charge is positive. When these cells are supplied with iodide ions, the iodide ions in the IHN-infected cells are de-electronized to produce eodo, and the infected cells are selectively inactivated by the bactericidal action of the eodo.

[0014] In the present invention, examples of ョ and Nal are given as examples of 化合物 or Nal as a compound that produces ョ or ion, but any compound that produces オン can be used as long as it is not limited to these. . Further, in the present invention, the antiviral activity can be further enhanced by combining a polyphenol compound such as humic acid, tea power techin, or the like with the anode. In the present invention, it is particularly preferable to use Nal in combination with humic acid and / or catechin. In the present invention, an antiviral composition can be prepared by combining these compounds with, for example, a pharmaceutically acceptable carrier. In addition, by blending this antiviral composition with any food, a functional food having an antiviral function can be obtained. Further, the antiviral composition can be used as an antiviral drug.

[0015] In the present invention, as shown in the test examples described later, a strong fish disease-causing IHN virus

Compared to (RNA-based) cultures, sodium eodo-sodium Z polyphenols (such as tea catechin or humic acid), which significantly reduced the titer and showed a significantly higher survival rate in the salmon infection test, It has been shown to have antiviral function. It is particularly noteworthy that both substances are commonplace and that the function is manifested when eaten. Utilizing the principle of the present invention, for example, a net charge in carrier-free electrophoresis of a cell is set as a marker, and a neutral charge is set at a normal point, and a positive / negative charge shift of a neutral point force is calculated. As a measure of the metabolic abnormalities of cells, it is possible to fractionate sample cells alive using an electrophoresis apparatus and measure their distribution. In addition, by screening a substance whose net charge of the cell system returns to a normal point, an active ingredient that controls cell abnormalities can be specified. Also

By evaluating the specific substance in a fish system (cells, fry), it is possible to perform low-cost and high-precision performance evaluation. Furthermore, abnormalities in living cells can be detected as normal and abnormalities in metabolism can be detected by measuring the charge. The system of the present invention is also noteworthy as a method that makes it possible to establish these evaluation methods.

[0016] Ode ions or olude ions to be incorporated into the antiviral composition of the present invention The amount of the compound to be produced is as long as the desired effect of the present invention is exhibited.

Although not particularly limited, it is usually preferable to add 0.01 to 10% by weight to the composition of the present invention. The antiviral composition of the present invention may contain diluents or excipients such as fillers, bulking agents, binders, humectants, disintegrants, surfactants, and lubricants which are usually used in pharmaceutical preparations. It is prepared using The form of this composition can be selected according to the purpose of treatment, and typical examples include tablets, pills, powders, solutions, suspensions, emulsions, granules, capsules, and injections (solutions). , Suspending agents, etc.).

[0017] The composition of the present invention may contain conventional additives such as vitamins, hormonal agents, pharmacologic agents such as amino acids, surfactants, pigments, dyes, pigments, fragrances, ultraviolet absorbers, humectants, and humectants. A tackifier, an antioxidant, a sequestering agent, a pH adjuster, and the like can be arbitrarily added as necessary. In the present invention, examples of the carrier include excipients such as glucose, lactose, starch, cocoa butter, hydrogenated vegetable oil, kaolin, and talc; binders such as gum arabic, tragacanth, gelatin, and ethanol; laminaran; And disintegrating agents such as agar. When used as an injection, the solvents, emulsions and suspensions are preferably sterile and isotonic with blood. In making these forms, for example, water, ethyl alcohol, propylene glycol, polyoxyethylene sorbitan fatty acid ester and the like can be used as the diluent.

[0018] The compounding method and administration method of the antiviral composition or the drug thereof of the present invention are not particularly limited, and are compounded by a method according to various formulation forms, patient age, gender and other conditions, degree of disease, and the like. Or administered. For example, tablets, pills, solutions, suspensions, emulsions, granules, and capsules are orally administered. The dose of the preparation of the present invention is appropriately selected depending on the usage, age of the patient, gender, other conditions, the degree of the disease, and the like.In general, the amount of the active ingredient compound is expressed as It is preferred that the amount be about 11 to 200 mg. In addition, it is preferable that the preparation is usually administered in 1 to 3 divided doses.

[0019] The antiviral composition of the present invention exhibits an excellent antiviral effect of selectively inactivating virus-infected cells, and has extremely low toxicity and excellent safety. is there. Further, the active ingredient such as the antiviral composition of the present invention is excellent in solubility in water and excellent in absorbability of intestinal tract. Further, the antiviral composition of the present invention And the like have the effect of selectively inactivating virus-infected cells, so that their formulation or administration can be extremely small. By blending the above antiviral composition of the present invention with food raw materials and / or processed foods, functional foods with an added antiviral function can be produced. The shape, form and the like are not particularly limited, and they can be arbitrarily designed.

The invention's effect

According to the present invention, (1) a novel antiviral composition effective for prevention and treatment of viral infections caused by various viruses such as AIDS virus can be provided, and (2) an antiviral function is added. (3) can provide an antiviral drug effective against infectious diseases caused by viruses such as AIDS virus, and (4) can provide virus-infected cells. New evil (1Z2I) that can be selectively deactivated

2 Generation ・ Delivery system can be provided.

BEST MODE FOR CARRYING OUT THE INVENTION

Next, the present invention will be specifically described based on test examples and examples, but the present invention is not limited at all by the following examples.

First, the present invention will be specifically described by an IHN virus infection suppression test.

Test example 1

In this test example, the antiviral (IHN virus) effect of tea catechin was tested. A MEM medium (minimum essential nutrient medium) containing 1 μg / ml¾V and 10 μg Zml of tea catechin was inoculated with 2 cells of RTG-2 cells derived from dimasu, and 0.1 I / cell was added to each cell. The cells were infected with the HN virus (moiO.1) and cultured at 15 ° C and pH 7.2 for 5 to 10 days. After the culture, the number of viruses (virus titer TCID Zml) was measured using the culture supernatant. That

50

The results are shown in Table 1.

[Table 1] Virus titer (log _{1 ()} TCID _{5 (1} / m 1)

[0024] Test Example 2

In this test example, the antiviral (IHN virus) effect of humic acid was tested.

A MEM medium (minimum essential nutrient medium) containing 1 μgZml of humic acid and 10 μgZml of humic acid was inoculated with RTG-2 cultured cells derived from dimasu, and 0.01 IHN / cell was further added. The virus (moiO.01) was infected and cultured at 15 ° C., pH 7.2 for 7 days. After the culture, the number of viruses (virus titer TCID / ml) was measured using the culture supernatant. Table 2 shows the results.

50

Show.

[Table 2] Virus titer (logi. TCID ₅ ; ml)

[0026] Test Example 3

In this test example, we tested the synergistic antiviral (IHN virus) effect of adding Nal to catechin, which is a tea catechin!

To a MEM medium containing 10 gZml of tea catechin or 1 μg / ml of humic acid (minimum essential nutrient medium), add 1, 5, 10, 20 / zgZml of Nal. RTG-2 cells were inoculated, and further infected with 0.01 IHN virus (moiO.01) per cell, and cultured at 15 ° C and pH 7.2 for 10 days. After the culture, the number of viruses (virus titer TCID / ml) was measured using the culture supernatant. The results are shown in Table 3.

50

[Table 3]

Virus titer (log ₁₀ TCID ₅ , ml)

Next, the present invention will be specifically described by an oral administration test and a toxicity test on juvenile salmon.

Test example 4

Two 60-liter aquariums contain 220 juvenile salmon trout with an average weight of 0.5 g, and feed humic acid and humic acid to one juvenile while supplying breeding water (groundwater) at 10 ° C at a rate of 2 liters Z. The Nal-mixed diet was fed, while the other fry were fed a normal diet without them as a control. Mixing 5 mg of humic acid and 50 mg of NaI per 100 g of small granular feed and coating with 5 g of feed oil prevents dissolution and diffusion of reagents into water. A solution containing the IHN virus (virus solution) was adjusted through a micropump to a virus count (virus titer) of 100 TCID Zml in breeding water, bred for 5 days, and infected with the virus. Bait

50

Helped four days before the virus infection. Fry deaths were recorded for 30 days. Figure 4 shows the results. Shown in

Test Example 5

Two 60-liter aquariums hold 220 juvenile salmon trout with an average weight of 0.3 g, and supply humic acid and humic acid to one juvenile while feeding breeding water (groundwater) at 10 ° C at a rate of 2 liters Z. The Nal-mixed diet was fed, while the other fry were fed a normal diet without them as a control. Mixing 5 mg of humic acid and 50 mg of NaI per 100 g of small granular feed and coating with 5 g of feed oil prevents dissolution and diffusion of reagents into water. A solution containing the IHN virus (virus solution) was adjusted through a micropump to a virus count (virus titer) of 100 TCID Zml in breeding water, bred for 5 days, and infected with the virus. Bait

50

Helped 20 days before the virus infection. Fry deaths were recorded for 30 days. Figure 5 shows the results.

[0030] Test example 6

Two 60-liter aquariums contain 220 fish salmon fry, averaging 0.6-0.9 g in weight, and feed breeding water (groundwater) at 10 ° C at a rate of 2 liters Z to one fry. Food mixed with humic acid and Nal was not mixed with the other fry as a control! Normal feed was given. A mixture of 10 mg of humic acid and NallOmg per 100 g of small granular feed was coated with 5 g of feed oil to prevent the reagent from dissolving and diffusing into water. A solution containing IHN virus (virus solution) is passed through a micropump and the number of viruses (virus titer) 100TCID

They were adjusted to 50 Zml breeding water, bred for 5 days, and infected with the virus. Food was given 20 days before virus infection. Fry deaths were recorded for 30 days. Figure 6 shows the results.

[0031] Test example 7

In this test example, a toxicity test was performed. Two 60-liter aquariums contain 220 fish salmon fry, averaging 0.3 g in weight, and feed breeding water (groundwater) at 10 ° C at a rate of 2 liters Z while humic acid and The Nal-mixed diet was given, while the other fry were given normal diet without control as a control. Mixing 10 mg of humic acid and NallOmg per 100 g of small granular feed and coating with 5 g of feed oil prevented dissolution and diffusion of reagents into water. Fry deaths were recorded for 30 days. Figure 7 shows the results. You.

[0032] From the above test results, (1) oral administration of humic acid and Nal has the effect of preventing virus infection against salmon, and (2) mixing of humic acid 5mgZlOOg diet and NaI50mgZlOOg diet has virus inhibitory effect. It was confirmed that, in terms of fish body weight, this amount was equivalent to 1.5 mg Zkg of humic acid diet and 15 mg Zkg of NaI, and (3) humic acid and Nal had no toxicity.

[0033] Test Example 8

Inoculate a microplate with 30 IX L / well of-ヮ fetal embryo fibroblast suspension X 10 ⁵ cells Zml), and inoculate Nal and green tea catechin (main component: epigallocatechin gallate) to a prescribed concentration. The cells were added after being adjusted (PH 7.4) with a culture medium, and further inoculated with an avian influenza virus solution whose virus titer had been measured in advance, and cultured at 37 ° C for 3-5 days.

[0034] After completion of the culture, the cells were stained with Alamar Blue, and the cytopathic effect (CPE) was observed under a microscope. Virus suppression effects were determined by CPE for Nal and catechin alone and in various combinations of both. The results are shown in Table 4 as "The virus-suppressing effect of aodeon and catechin on avian influenza virus".

[Table 4]

Cytopathic effect (CPE) of host cells

Dilution of virus solution

Iodo-ion and / or catechin 10 ° 10 '10 ² 10 ³ 10 ⁴ 10 ⁵ 10 ⁶

Na [1 jt g / ml ++++ ++++ ++++ + -H- ++--

Nal 1 0 U g / ml ++++ ++++ ++++ +++---

Catechin 1 fi g / ml ++++ l i l t ++++ +++ ++--

Catechin 10 0 U g / ml ++++ ++++ +++ -H- +---

Nal 1 g / ml + Catechin 1 μg / ml + ++++ M M -H- + +--

Nal 10 g / ml + Catechin 1 U g / ml ++++ ++++ ++ + ++---

Nal 1 g / ml + Catechin 10 U g / ml ++++ ++++ +++ -H----

Nal 5 g / ml + Catechin l O jU / ml M M 1 ++ ++++ ++ one--

Na 【10 g / ml + Catechin 10 g / ml M M M i ++++++--

Virus alone (= positive control) ++++ + 1 1 ++++ + -H--No virus (= negative control)

* CPE; ++++> +++> ++> +>-"^ Effective on virus

[0036] Test Example 9 A microplate is inoculated with 30 μL of chicken fetal fibroblast cell suspension X 10 ⁵ cells Zml), and the cell culture medium is adjusted to a prescribed concentration of Nal and green tea catechin (main component: epigallocatechin gallate). 37. Adjusted pH (pH 7.4) and spiked, then inoculated with a solution of Avian Newcastle disease virus whose virus titer had been measured in advance. C. The cells were cultured for 35 days.

[0037] After completion of the culture, the cells were stained with Alamar Blue, and the cytopathic effect (CPE) was observed under a microscope. Virus suppression effects were determined by CPE for Nal and catechin alone and in various combinations of both. The results are shown in Table 5 as "The virus-suppressing effect of aodeon and catechin on avian Newcastle disease virus".

[0038] [Table 5]

Cytopathic effect (CPE) of host cells

Dilution of virus solution

lodo-ιοη and / or catechin 10 ° 10 ¹ 10 ² 10 ³ 10 ⁴ 10 ⁵ 10 ⁶

Nal 1 U g / ml ■ H- ■ H-H- ++

Nal \ Q u g / ml +++

Catechin 1 g / ml -H-++

Catechin 10 g / ml -HH-++

Nal 1 jt g / ml + Catechin 1 U g / ml ■ H- + ■ H- +

Nal 10 i g / ml + Catechin 1 g / ml +++ -H-

Nal 1 U g / ml + Catechin 10 0 g / ml +++ +++

Nal 5 jU g / ml + Catechin 10 jU g / ml + -H-

Nal 10 / ml + Catechin 10 g / mlH-H- -H-

Virus alone (= positive control) + H-H- ++

No virus (= negative control)

CPE; ++++> +++> ++>>

Effective on virus

[0039] Test example 10

Was inoculated 30 IX L / Ueru of Niwatori fetal fibroblast cell suspension X 10 ⁵ cells ZML) microplates, Nal and green tea catechins (ingredient: the E pin gallocatechin gallate) to a predetermined concentration cell culture The mixture was adjusted with a medium (pH 7.4), supplemented with cauliflower, and inoculated with a solution of Avian Newcastle Disease Virus, whose virus titer had been previously measured, for infection, and cultured at 37 ° C for 35 days.

After completion of the culture, the cells were stained with Alamar Blue and the absorbance (OD) of the microplate was measured using a microplate reader. The survival rate was determined. The results are shown in FIG.

Test Example 11

(1) Test 1

A microplate is inoculated with 30 μL / well of MDBK cell suspension (1 × 10 ⁵ cells Zml) of Nishi and green tea catechin (main component: epigallocatechin gallate) at a specified concentration. Cell culture medium (pH 7.4), add, and inoculate with a Psivirus diarrhea virus solution whose whisker value has been measured in advance, and incubate for 3-5 days at 37 ° C. did.

[0042] After completion of the culture, the cells were stained with Alamar Blue, and the cytopathic effect (CPE) was observed under a microscope. Virus suppression effects were determined by CPE for Nal and catechin alone and in various combinations of both. The results are shown in the upper part of Table 6 as "Effective effect of odo'catechin on virus-infected mammalian cells".

(2) Test 2

A microplate is inoculated with 30 L / well of a feline renal fibroblast suspension (1 × 10 ⁵ cells / ml), and Nal and green tea catechin (main component: epigallocatechin gallate) are in a prescribed concentration. The cells were adjusted with cell culture medium (PH 7.4), added, and inoculated with a feline virulent virus solution whose virus titer had been measured in advance, and cultured at 37 ° C for 3-5 days.

[0044] After completion of the culture, the cells were stained with Alamar Blue, and the cytopathic effect (CPE) was observed under a microscope. Virus suppression effects were determined by CPE for Nal and catechin alone and in various combinations of both. The results are shown in the lower part of Table 6 as "Effective effect of eodo'catechin on virus-infected mammalian cells".

[Table 6] Viral titer Animal virus Nal concentration · Viscosity (logio TCIDso / ml) Psivirus diarrhea virus 1 10/1 ^ + catechin 10 ^ ¾ ^ 1 10 ^{5 0}

Control (no Nal, without catechin) 10 ^{^6.5}

Reduce virus to tens of minutes ¾

Cytopathic effect (CPE)

Dilution of virus solution

Nal and catechin 10 ⁵ 10 ⁶

Nal 10 g / ml + catechin 10 μg / mi +++ ——

Control (virus alone) +++--+ Feline riculovirus Nal 10 i / g / ml + Catechin 10 g ml 10 ^{6 5}

Control (no Nal, without catechin) 10 ⁷ - ⁵

Reduce virus by 1/10 a

[0046] Manufacturing example

5 mg Zl00 g raw material and NaI 150 mg ZlOOg raw material are blended with ordinary bakery raw material and mixed with an antiviral composition that also has the power of the raw material, and bread products are manufactured in a conventional manner to produce the intended functional food. Manufactured.

Industrial applicability

[0047] As described in detail above, the present invention relates to an antiviral composition and the like, and according to the present invention, a novel antiviral composition which is extremely effective in preventing and treating infectious diseases caused by viruses such as AIDS virus. Functional foods having antiviral functions, antiviral preparations, etc. can be manufactured and provided. It is possible to provide a novel antiviral drug having an action of selectively inactivating virus-infected cells. Further, according to the present invention, for example, a functional food to which an anti-HIV virus activity is added can be provided. Elucidation of the mechanism of cell death in various virus-infected cells and the pathogenesis of new virus infections can be achieved by utilizing the principle of the present invention, which uses the "degeneration system using eodoion in virus-infected cells". It can contribute to the establishment of an evaluation method. Since the antiviral preparation of the present invention is inexpensive and highly safe, it is possible to quickly and urgently respond to and contribute to AIDS relief activities in South Africa and the like by using the antiviral preparation. it can. Brief Description of Drawings

FIG. 1 shows a top view and a cross-sectional view of an electrophoresis apparatus used in a test example of the present invention. FIG. 2 shows an electrophoretogram of virus-infected RTG- ² cells.

FIG. 3 shows electropherograms of RTG-2 cells and CHSE-214 cells.

FIG. 4 shows the results of an oral administration test to juvenile salmon in Test Example 4.

FIG. 5 shows the results of an oral administration test to juvenile salmon trout in Test Example 5.

FIG. 6 shows the results of an oral administration test to juvenile salmon trout in Test Example 6.

[7] Shows the results of the toxicity test.

[8] This section shows the test procedure and the cell survival effect of bode ion and tea catechin on chicken fetal fibroblasts infected with avian-Eucasian disease virus.

Claims

The scope of the claims

[1] An antiviral composition having an action of selectively inactivating virus-infected cells, characterized by containing, as an active ingredient, an iodine ion or a compound that produces an iodine ion.

[2] The antiviral composition according to claim 1, wherein a combination of an anode and a polyphenol conjugate is used.

[3] A functional food having an antiviral function, wherein the antiviral composition according to claim 1 or 2 is blended to add an antiviral function.

[4] An antiviral agent having an action of selectively inactivating virus-infected cells, characterized in that the antiviral composition according to claim 1 or 2 is used.

[5] Supplying an anode or a compound generating an anode to a living cell system containing a virus-infected cell to specifically produce an anode (1Z2I) in the virus-infected cell,

2

To selectively inactivate and kill virus-infected cells by the action of the drug (1Z2I)

2

A method for selectively inactivating virus-infected cells, characterized in that:

[6] It is necessary to supply an olive ion or a compound that produces an iodide ion to a living cell system containing a virus-infected cell to specifically produce an eodo (1Z2I) in the virus-infected cell.

2

Characteristic selective generation of (1Z2I) in virus-infected cells' Derivative cis

2

Tem.