WO2005083092A3 - Transposon from aspergillus fumatigus and mutagenesis therewith - Google Patents

Transposon from aspergillus fumatigus and mutagenesis therewith Download PDF

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Publication number
WO2005083092A3
WO2005083092A3 PCT/GB2005/000743 GB2005000743W WO2005083092A3 WO 2005083092 A3 WO2005083092 A3 WO 2005083092A3 GB 2005000743 W GB2005000743 W GB 2005000743W WO 2005083092 A3 WO2005083092 A3 WO 2005083092A3
Authority
WO
WIPO (PCT)
Prior art keywords
transposon
fumatigus
aspergillus
mutagenesis
therewith
Prior art date
Application number
PCT/GB2005/000743
Other languages
French (fr)
Other versions
WO2005083092A2 (en
Inventor
Michael John Bromley
Peter Hey
Original Assignee
F2G Ltd
Michael John Bromley
Peter Hey
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F2G Ltd, Michael John Bromley, Peter Hey filed Critical F2G Ltd
Publication of WO2005083092A2 publication Critical patent/WO2005083092A2/en
Publication of WO2005083092A3 publication Critical patent/WO2005083092A3/en

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102Mutagenizing nucleic acids
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/38Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from Aspergillus

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Mycology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A nucleic acid or transposable element comprising one, two or more regions, wherein each region is selected from: (i) the sequence SEQ ID NO:l, 3, or 8, or (ii) a sequence which has at least 50 % identity with (i), or (iii) a sequence of at least 50 bases which is a fragment of (i) or (ii), wherein the nucleic acid or transposable element or sequence optionally has transposon activity.
PCT/GB2005/000743 2004-02-27 2005-02-28 Transposon from aspergillus fumatigus and mutagenesis therewith WO2005083092A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0404433.5 2004-02-27
GB0404433A GB0404433D0 (en) 2004-02-27 2004-02-27 Transposon mutagenesis

Publications (2)

Publication Number Publication Date
WO2005083092A2 WO2005083092A2 (en) 2005-09-09
WO2005083092A3 true WO2005083092A3 (en) 2005-12-15

Family

ID=32051023

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2005/000743 WO2005083092A2 (en) 2004-02-27 2005-02-28 Transposon from aspergillus fumatigus and mutagenesis therewith

Country Status (2)

Country Link
GB (1) GB0404433D0 (en)
WO (1) WO2005083092A2 (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998008960A1 (en) * 1996-08-26 1998-03-05 Genencor International, Inc. IDENTIFICATION OF AND CLONING A MOBILE TRANSPOSON FROM $i(ASPERGILLUS)
WO2004067709A2 (en) * 2003-01-17 2004-08-12 Elitra Pharmaceuticals, Inc. Identification of essential genes of aspergillus fumigatus and methods of use

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998008960A1 (en) * 1996-08-26 1998-03-05 Genencor International, Inc. IDENTIFICATION OF AND CLONING A MOBILE TRANSPOSON FROM $i(ASPERGILLUS)
WO2004067709A2 (en) * 2003-01-17 2004-08-12 Elitra Pharmaceuticals, Inc. Identification of essential genes of aspergillus fumigatus and methods of use

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
DATABASE EMBL [online] 24 June 1997 (1997-06-24), "Aspergillus niger transposon Ant1 putative Tc1-mariner class transposase gene, complete cds.", XP002344795, retrieved from EBI accession no. EM_FUN:S80872 Database accession no. S80872 *
DATABASE EMBL [online] 8 January 2003 (2003-01-08), "Aspergillus oryzae transposon Aot1 DNA, complete sequence.", XP002344796, retrieved from EBI accession no. EM_FUN:AB072434 Database accession no. AB072434 *
DATABASE EMBL [online] 8 June 2000 (2000-06-08), "Aspergillus oryzae transposon-like element Tao1 pseudogene for transposase.", XP002344793, retrieved from EBI accession no. EM_FUN:AB021710 Database accession no. AB021710 *
DATABASE EMBL [online] 9 March 1997 (1997-03-09), "Emericella nidulans cosmid SW06E08, complete sequence.", XP002344794, retrieved from EBI accession no. EM_FUN:ENAC133 Database accession no. AC000133 *
DATABASE Geneseq [online] 4 November 2004 (2004-11-04), "Aspergillus fumigatus essential gene genomic sequence #316.", XP002344797, retrieved from EBI accession no. GSN:ADR84505 Database accession no. ADR84505 *
DATABASE Geneseq [online] 4 November 2004 (2004-11-04), "Aspergillus fumigatus essential gene genomic sequence #492.", XP002344798, retrieved from EBI accession no. GSN:ADR84681 Database accession no. ADR84681 *
GLAYZER D C ET AL: "THE ISOLATION OF ANT1, A TRANSPORTABLE ELEMENT FROM ASPERGILLUS NIGER", MOLECULAR AND GENERAL GENETICS, SPRINGER VERLAG, BERLIN, DE, vol. 249, no. 4, 10 December 1995 (1995-12-10), pages 432 - 438, XP002049285, ISSN: 0026-8925 *
MONROY F ET AL: "Taf1: A class II transposon of Aspergillus fumigatus", FUNGAL GENETICS AND BIOLOGY, SAN DIEGO, CA, US, vol. 42, no. 7, July 2005 (2005-07-01), pages 638 - 645, XP004930795, ISSN: 1087-1845 *

Also Published As

Publication number Publication date
WO2005083092A2 (en) 2005-09-09
GB0404433D0 (en) 2004-03-31

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