WO2005081716A3 - DNA VACCINES TARGETING ANTIGENS OF THE SEVERE ACUTE RESPIRATORY SYNDROME CORONAVIRUS (SARS-CoV) - Google Patents

DNA VACCINES TARGETING ANTIGENS OF THE SEVERE ACUTE RESPIRATORY SYNDROME CORONAVIRUS (SARS-CoV) Download PDF

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WO2005081716A3
WO2005081716A3 PCT/US2004/039579 US2004039579W WO2005081716A3 WO 2005081716 A3 WO2005081716 A3 WO 2005081716A3 US 2004039579 W US2004039579 W US 2004039579W WO 2005081716 A3 WO2005081716 A3 WO 2005081716A3
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Prior art keywords
sars
cov
responses
polypeptides
acute respiratory
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PCT/US2004/039579
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French (fr)
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WO2005081716A2 (en
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Tzyy-Choou Wu
Chien-Fu Hung
Tae Woo Kim
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Univ Johns Hopkins
Tzyy-Choou Wu
Chien-Fu Hung
Tae Woo Kim
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Application filed by Univ Johns Hopkins, Tzyy-Choou Wu, Chien-Fu Hung, Tae Woo Kim filed Critical Univ Johns Hopkins
Publication of WO2005081716A2 publication Critical patent/WO2005081716A2/en
Publication of WO2005081716A3 publication Critical patent/WO2005081716A3/en

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    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/0005—Vertebrate antigens
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
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    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4725—Proteoglycans, e.g. aggreccan
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/545—Medicinal preparations containing antigens or antibodies characterised by the dose, timing or administration schedule
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/555—Medicinal preparations containing antigens or antibodies characterised by a specific combination antigen/adjuvant
    • A61K2039/55511—Organic adjuvants
    • A61K2039/55555—Liposomes; Vesicles, e.g. nanoparticles; Spheres, e.g. nanospheres; Polymers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/57—Medicinal preparations containing antigens or antibodies characterised by the type of response, e.g. Th1, Th2
    • A61K2039/572—Medicinal preparations containing antigens or antibodies characterised by the type of response, e.g. Th1, Th2 cytotoxic response
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
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    • A61K2039/575—Medicinal preparations containing antigens or antibodies characterised by the type of response, e.g. Th1, Th2 humoral response
    • A—HUMAN NECESSITIES
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    • A61K2039/58—Medicinal preparations containing antigens or antibodies raising an immune response against a target which is not the antigen used for immunisation
    • A61K2039/585—Medicinal preparations containing antigens or antibodies raising an immune response against a target which is not the antigen used for immunisation wherein the target is cancer
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    • C12N2710/24111—Orthopoxvirus, e.g. vaccinia virus, variola
    • C12N2710/24141—Use of virus, viral particle or viral elements as a vector
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    • C12N2770/00011—Details
    • C12N2770/20011—Coronaviridae
    • C12N2770/20034—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein

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  • Health & Medical Sciences (AREA)
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  • Virology (AREA)
  • Oncology (AREA)
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  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
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Abstract

This invention provides compositions and methods for inducing and enhancing immune responses, particularly antigen-specific CD8+ T cell mediated responses, against antigens of the SARS coronavirus. These antigens include epitopes of the Membrane (M), Envelope (E), Spike (S) and Nucleocapsid (N) proteins of the virus. Such responses are induced using DNA constructs as an immunogens or vaccines, which encode chimeric polypeptides comprising endoplasmic reticulum chaperone polypeptides, such as human calreticulin (CRT) and an antigenic peptide or polypeptide. In particular, the invention provides compositions and methods for enhancing immune responses induced by polypeptides made in vivo by administered nucleic acid, such as naked DNA or expression vectors, encoding the chimeric molecules. Such enhanced immunity, whether T cell mediated or antibody-mediated protects an infected subject from infection or spread of the SARS CoV in vivo.
PCT/US2004/039579 2003-11-24 2004-11-24 DNA VACCINES TARGETING ANTIGENS OF THE SEVERE ACUTE RESPIRATORY SYNDROME CORONAVIRUS (SARS-CoV) WO2005081716A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US52447903P 2003-11-24 2003-11-24
US60/524,479 2003-11-24

Publications (2)

Publication Number Publication Date
WO2005081716A2 WO2005081716A2 (en) 2005-09-09
WO2005081716A3 true WO2005081716A3 (en) 2009-04-02

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Families Citing this family (21)

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US8128922B2 (en) 1999-10-20 2012-03-06 Johns Hopkins University Superior molecular vaccine linking the translocation domain of a bacterial toxin to an antigen
JP5087201B2 (en) 2000-08-03 2012-12-05 ジョンズ・ホプキンス・ユニバーシティ Molecular vaccine with endoplasmic reticulum chaperone polypeptide linked to antigen
AU2002242016A1 (en) 2001-02-01 2002-08-12 The Johns Hopkins University Nucleic acid derived vaccine that encodes an antigen linked to a polypeptide that promotes antigen presentation
US9701725B2 (en) 2003-05-05 2017-07-11 The Johns Hopkins University Anti-cancer DNA vaccine employing plasmids encoding signal sequence, mutant oncoprotein antigen, and heat shock protein
CA2594040A1 (en) 2005-01-06 2006-07-13 The Johns Hopkins University Rna interference that blocks expression of pro-apoptotic proteins potentiates immunity induced by dna and transfected dendritic cell vaccines
WO2006095180A2 (en) * 2005-03-10 2006-09-14 Ultra Biotech Limited Humananized monoclonal antibodies against sars - associated coronavirus and treatment of patients with sars
US9085638B2 (en) 2007-03-07 2015-07-21 The Johns Hopkins University DNA vaccine enhancement with MHC class II activators
CN101915848B (en) * 2010-07-06 2013-09-25 中国医学科学院病原生物学研究所 Application of RBD protein in preparation of auxiliary diagnostic agent for SARS patients
US9884895B2 (en) 2014-03-20 2018-02-06 The University Of North Carolina At Chapel Hill Methods and compositions for chimeric coronavirus spike proteins
JP2020534352A (en) 2017-09-07 2020-11-26 キュー バイオファーマ，インコーポレーテッド T cell regulatory multimeric polypeptide with conjugation site and how to use it
US20210046177A1 (en) * 2018-01-26 2021-02-18 Nantcell, Inc. Compositions and methods for combination cancer vaccine and immunologic adjuvant therapy
CN111196857B (en) * 2020-02-05 2023-07-21 杭州贤至生物科技有限公司 Novel coronavirus multi-epitope recombinant antigen and preparation method thereof
US20230117521A1 (en) * 2020-03-25 2023-04-20 Cue Biopharma, Inc. T-cell modulatory multimeric polypeptides with conjugation sites and methods of use thereof
US20230210978A1 (en) * 2020-04-02 2023-07-06 Francois Binette A universal adoptive immunotherapy approach to treat covid-19 and future emerging infectious diseases
CN111440229B (en) * 2020-04-13 2021-08-03 中国人民解放军军事科学院军事医学研究院 Novel coronavirus T cell epitope and application thereof
WO2021214248A1 (en) * 2020-04-23 2021-10-28 F. Hoffmann-La Roche Ag Corona nucleocapsid antigen for use in antibody-immunoassays
US20230181720A1 (en) * 2020-05-18 2023-06-15 The Regents Of Th University Of California Safe potent single vector platform vaccine against covid-19
WO2021248101A1 (en) * 2020-06-05 2021-12-09 Epicentrx, Inc. Mono- and multi-valent sars-cov-2 adenoviral vector vaccines and sars-cov-2 immune globulin and methods of use
CN111978376B (en) * 2020-06-15 2022-02-18 江苏安泰生物技术有限公司 Pharmaceutical composition for preventing and/or treating coronavirus infection, and preparation method and application thereof
WO2022026921A1 (en) * 2020-07-30 2022-02-03 Repertoire Immune Medicines, Inc. Identification and use of t cell epitopes in designing diagnostic and therapeutic approaches for covid-19
TWI839716B (en) * 2021-05-18 2024-04-21 法信諾生醫股份有限公司 Peptide vaccine for virus infection

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Patent Citations (1)

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Non-Patent Citations (2)

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