WO2005080992A3 - P-glycoprotein assay - Google Patents

P-glycoprotein assay

Info

Publication number
WO2005080992A3
WO2005080992A3 PCT/GB2005/000614 GB2005000614W WO2005080992A3 WO 2005080992 A3 WO2005080992 A3 WO 2005080992A3 GB 2005000614 W GB2005000614 W GB 2005000614W WO 2005080992 A3 WO2005080992 A3 WO 2005080992A3
Authority
WO
WIPO (PCT)
Prior art keywords
glycoprotein
assay
binding
test agent
ligand
Prior art date
Application number
PCT/GB2005/000614
Other languages
French (fr)
Other versions
WO2005080992A2 (en
Inventor
Jenny Berry
Original Assignee
Amersham Biosciences Uk Ltd
Jenny Berry
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amersham Biosciences Uk Ltd, Jenny Berry filed Critical Amersham Biosciences Uk Ltd
Publication of WO2005080992A2 publication Critical patent/WO2005080992A2/en
Publication of WO2005080992A3 publication Critical patent/WO2005080992A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/60—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances involving radioactive labelled substances
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention relates to a radio-ligand method for measuring the binding of a test agent to a P-glycoprotein preparation. The invention is a homogeneous scintillation proximity assay for quantifying the binding of a ligand and a test agent to a P-glycoprotein preparation. The assay is suitable for high-throughput screening to identify novel substrates/ inhibitors of P-glycoprotein.
PCT/GB2005/000614 2004-02-21 2005-02-21 P-glycoprotein assay WO2005080992A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0403913.7 2004-02-21
GB0403913A GB0403913D0 (en) 2004-02-21 2004-02-21 P-glycoprotein assay

Publications (2)

Publication Number Publication Date
WO2005080992A2 WO2005080992A2 (en) 2005-09-01
WO2005080992A3 true WO2005080992A3 (en) 2005-12-08

Family

ID=32040164

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2005/000614 WO2005080992A2 (en) 2004-02-21 2005-02-21 P-glycoprotein assay

Country Status (2)

Country Link
GB (1) GB0403913D0 (en)
WO (1) WO2005080992A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007024678A2 (en) * 2005-08-19 2007-03-01 Bristol-Myers Squibb Company Scintillation proximity assay for the identification of p-glycoprotein modulators
US7989630B2 (en) 2008-04-30 2011-08-02 National Institutes Of Health Represented By The Secretary Of The Department Of Health And Human Services, National Institutes Of Health Radiotracers for imaging P-glycoprotein function

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6524786B1 (en) * 1997-08-18 2003-02-25 Amersham Plc Scintillation proximity test
US20030059857A1 (en) * 2001-09-17 2003-03-27 Schering Corporation G-protein coupled receptor and methods

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6524786B1 (en) * 1997-08-18 2003-02-25 Amersham Plc Scintillation proximity test
US20030059857A1 (en) * 2001-09-17 2003-03-27 Schering Corporation G-protein coupled receptor and methods

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DOPPENSCHMITT STEPHAN ET AL: "Radioligand-binding assay employing P-glycoprotein-overexpressing cells: Testing drug affinities to the secretory intestinal multidrug transporter", PHARMACEUTICAL RESEARCH (NEW YORK), vol. 15, no. 7, July 1998 (1998-07-01), pages 1001 - 1006, XP002335682, ISSN: 0724-8741 *
GAO JINNIAN ET AL: "A functional assay for quantitation of the apparent affinities of ligands of P-glycoprotein in Caco-2 cells", PHARMACEUTICAL RESEARCH (NEW YORK), vol. 18, no. 2, February 2001 (2001-02-01), pages 171 - 176, XP002335681, ISSN: 0724-8741 *
GRAVES R ET AL: "An homogeneous assay for measuring the uptake and efflux of radiolabelled drugs in adherent cells", JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, vol. 34, no. 3, 1997, pages 177 - 187, XP002335680, ISSN: 0165-022X *

Also Published As

Publication number Publication date
GB0403913D0 (en) 2004-03-24
WO2005080992A2 (en) 2005-09-01

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