WO2005076216A3 - Method and system for automaticed digital image based flourescent in situ hybridization (fish) analysis - Google Patents

Method and system for automaticed digital image based flourescent in situ hybridization (fish) analysis Download PDF

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Publication number
WO2005076216A3
WO2005076216A3 PCT/US2005/003272 US2005003272W WO2005076216A3 WO 2005076216 A3 WO2005076216 A3 WO 2005076216A3 US 2005003272 W US2005003272 W US 2005003272W WO 2005076216 A3 WO2005076216 A3 WO 2005076216A3
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WO
WIPO (PCT)
Prior art keywords
plural
fish
situ hybridization
digital image
fluorescent
Prior art date
Application number
PCT/US2005/003272
Other languages
French (fr)
Other versions
WO2005076216A2 (en
Inventor
Abhijeet S Gholap
Gauri A Gholap
C K V Rao
Madhura Vipra
Deepak K Pardeshi
Prithviraj Jadhav
Jayant Abhyankar
Original Assignee
Bioimagene Inc
Abhijeet S Gholap
Gauri A Gholap
C K V Rao
Madhura Vipra
Deepak K Pardeshi
Prithviraj Jadhav
Jayant Abhyankar
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/938,314 external-priority patent/US20050136509A1/en
Priority claimed from US10/966,071 external-priority patent/US20050136549A1/en
Application filed by Bioimagene Inc, Abhijeet S Gholap, Gauri A Gholap, C K V Rao, Madhura Vipra, Deepak K Pardeshi, Prithviraj Jadhav, Jayant Abhyankar filed Critical Bioimagene Inc
Publication of WO2005076216A2 publication Critical patent/WO2005076216A2/en
Publication of WO2005076216A3 publication Critical patent/WO2005076216A3/en

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Classifications

    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts

Abstract

A method and system for automated digital fluorescent in situ hybridization (FISH) image analysis. Luminance parameters from a digital image of a biological tissue sample to which a fluorescent compound (e.g., LSI-HER-2/neu and CEP-17 dyes) have been applied are analyzed to determine plural regions of interest. Fluorescent color signals in the plural regions of interest including plural cell nuclei are identified, classified and grouped into plural groups. Each of the plural groups is validated based on pre-defined conditions. A medical diagnosis or prognosis or medical, life science or biotechnology experiment conclusion determined using a count of plural ratios of validated fluorescent color signals within each of the cell nuclei within the plural groups.
PCT/US2005/003272 2004-02-03 2005-02-03 Method and system for automaticed digital image based flourescent in situ hybridization (fish) analysis WO2005076216A2 (en)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US54130104P 2004-02-03 2004-02-03
US60/541,301 2004-02-03
US10/938,314 US20050136509A1 (en) 2003-09-10 2004-09-10 Method and system for quantitatively analyzing biological samples
US10/938,314 2004-09-10
US10/966,071 2004-10-15
US10/966,071 US20050136549A1 (en) 2003-10-30 2004-10-15 Method and system for automatically determining diagnostic saliency of digital images

Publications (2)

Publication Number Publication Date
WO2005076216A2 WO2005076216A2 (en) 2005-08-18
WO2005076216A3 true WO2005076216A3 (en) 2005-10-27

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/003272 WO2005076216A2 (en) 2004-02-03 2005-02-03 Method and system for automaticed digital image based flourescent in situ hybridization (fish) analysis

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WO (1) WO2005076216A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9240043B2 (en) 2008-09-16 2016-01-19 Novartis Ag Reproducible quantification of biomarker expression

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008031228A1 (en) * 2006-09-15 2008-03-20 The Governors Of The University Of Alberta Automated fish analysis, circulating microfluidic chip, and method for immobilizing cells to a microfluidic chip
ES2324896B1 (en) * 2006-10-27 2010-05-24 Universidad Del Pais Vasco-Euskal Herriko Unibertsitatea SAMPLE IDENTIFICATION METHOD AND SYSTEM USED.
JP5425762B2 (en) 2007-05-14 2014-02-26 ヒストロックス，インコーポレイテッド． Compartment separation by pixel evaluation using image data clustering
CA2604317C (en) 2007-08-06 2017-02-28 Historx, Inc. Methods and system for validating sample images for quantitative immunoassays
KR20140120321A (en) * 2012-02-01 2014-10-13 벤타나 메디컬 시스템즈, 인코포레이티드 System for detecting genes in tissue samples
US9135694B2 (en) * 2012-12-04 2015-09-15 General Electric Company Systems and methods for using an immunostaining mask to selectively refine ISH analysis results

Citations (2)

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Publication number Priority date Publication date Assignee Title
US5427910A (en) * 1992-12-09 1995-06-27 Compucyte Corporation Method of cytogenetic analysis
WO2001020044A2 (en) * 1999-09-17 2001-03-22 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health & Human Services, The National Institutes Of Health Signal counting for in situ hybridization

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5427910A (en) * 1992-12-09 1995-06-27 Compucyte Corporation Method of cytogenetic analysis
WO2001020044A2 (en) * 1999-09-17 2001-03-22 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health & Human Services, The National Institutes Of Health Signal counting for in situ hybridization

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
KOZUBEK M ET AL: "High-resolution cytometry of FISH dots in interphase cell nuclei.", CYTOMETRY : THE JOURNAL OF THE SOCIETY FOR ANALYTICAL CYTOLOGY. 1 AUG 1999, vol. 36, no. 4, 1 August 1999 (1999-08-01), pages 279 - 293, XP002341432, ISSN: 0196-4763 *
LERNER B ET AL: "Automatic signal classification in fluorescence in situ hybridization images.", CYTOMETRY : THE JOURNAL OF THE SOCIETY FOR ANALYTICAL CYTOLOGY. 1 FEB 2001, vol. 43, no. 2, 1 February 2001 (2001-02-01), pages 87 - 93, XP002341433, ISSN: 0196-4763 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9240043B2 (en) 2008-09-16 2016-01-19 Novartis Ag Reproducible quantification of biomarker expression

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