WO2005072087A3 - System and methods for nucleic acid and polypeptide selection - Google Patents

System and methods for nucleic acid and polypeptide selection Download PDF

Info

Publication number
WO2005072087A3
WO2005072087A3 PCT/US2004/041380 US2004041380W WO2005072087A3 WO 2005072087 A3 WO2005072087 A3 WO 2005072087A3 US 2004041380 W US2004041380 W US 2004041380W WO 2005072087 A3 WO2005072087 A3 WO 2005072087A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
methods
cognate pair
protein
crosslink
Prior art date
Application number
PCT/US2004/041380
Other languages
French (fr)
Other versions
WO2005072087A2 (en
Inventor
Richard B Williams
Original Assignee
Proteonova Inc
Richard B Williams
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/847,087 external-priority patent/US7410761B2/en
Priority claimed from US10/847,484 external-priority patent/US20040229271A1/en
Application filed by Proteonova Inc, Richard B Williams filed Critical Proteonova Inc
Priority to CA002548465A priority Critical patent/CA2548465A1/en
Priority to EP04821258A priority patent/EP1692155A4/en
Priority to AU2004314705A priority patent/AU2004314705A1/en
Priority to JP2006544008A priority patent/JP2007513631A/en
Publication of WO2005072087A2 publication Critical patent/WO2005072087A2/en
Publication of WO2005072087A3 publication Critical patent/WO2005072087A3/en
Priority to US11/415,844 priority patent/US20070015181A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00—Drugs for disorders of the respiratory system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1062—Isolating an individual clone by screening libraries mRNA-Display, e.g. polypeptide and encoding template are connected covalently

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Public Health (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biophysics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Pulmonology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

This invention relates generally to systems and methods for identifying and selecting, desired proteins or nucleic acid molecules by linking mRNA, with known or unknown sequences, to its translated protein to form a cognate pair. The cognate pair is selected based upon desired properties of the protein or the nucleic acid. This method also includes the evolution of a desired protein or nucleic acid molecule by amplifying the nucleic acid portion of the selected cognate pair, introducing variation into the nucleic acid, translating the nucleic acid, attaching the nucleic acid to its protein to form a second cognate pair, and re-selecting this cognate pair based upon desired properties. Modified mRNAs operable to crosslink to tRNAs are also provided. Methods of producing a psoralen monoadduct or a crosslink are also provided. Methods of producing mRNA libraries and vaccines are also provided.
PCT/US2004/041380 2000-05-19 2004-12-10 System and methods for nucleic acid and polypeptide selection WO2005072087A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CA002548465A CA2548465A1 (en) 2003-12-12 2004-12-10 System and methods for nucleic acid and polypeptide selection
EP04821258A EP1692155A4 (en) 2003-12-12 2004-12-10 System and methods for nucleic acid and polypeptide selection
AU2004314705A AU2004314705A1 (en) 2003-12-12 2004-12-10 System and methods for nucleic acid and polypeptide selection
JP2006544008A JP2007513631A (en) 2003-12-12 2004-12-10 Systems and methods for selecting nucleic acids and polypeptides
US11/415,844 US20070015181A1 (en) 2000-05-19 2006-05-02 System and methods for nucleic acid and polypeptide selection

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US52933103P 2003-12-12 2003-12-12
US60/529,331 2003-12-12
US10/847,484 2004-05-17
US10/847,087 US7410761B2 (en) 2000-05-19 2004-05-17 System for rapid identification and selection of nucleic acids and polypeptides, and method thereof
US10/847,484 US20040229271A1 (en) 2000-05-19 2004-05-17 Compositions and methods for the identification and selection of nucleic acids and polypeptides
US10/847,087 2004-05-17
US62570704P 2004-11-05 2004-11-05
US60/625,707 2004-11-05

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US11/415,844 Continuation US20070015181A1 (en) 2000-05-19 2006-05-02 System and methods for nucleic acid and polypeptide selection

Publications (2)

Publication Number Publication Date
WO2005072087A2 WO2005072087A2 (en) 2005-08-11
WO2005072087A3 true WO2005072087A3 (en) 2005-11-03

Family

ID=34831414

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/041380 WO2005072087A2 (en) 2000-05-19 2004-12-10 System and methods for nucleic acid and polypeptide selection

Country Status (5)

Country Link
EP (1) EP1692155A4 (en)
JP (1) JP2007513631A (en)
AU (1) AU2004314705A1 (en)
CA (1) CA2548465A1 (en)
WO (1) WO2005072087A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SI3611266T1 (en) * 2005-08-23 2023-02-28 The Trustees Of The University Of Pennsylvania Rna containing modified nucleosides and methods of use thereof
JP5084012B2 (en) * 2007-01-17 2012-11-28 国立大学法人福井大学 Idiotype antigen carrier and idiotype vaccine using the same
AU2008216303A1 (en) * 2007-02-12 2008-08-21 Proteonova, Inc. Generation of library of soluble random polypeptides linked to mRNA

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5688670A (en) * 1994-09-01 1997-11-18 The General Hospital Corporation Self-modifying RNA molecules and methods of making
US5843701A (en) * 1990-08-02 1998-12-01 Nexstar Pharmaceticals, Inc. Systematic polypeptide evolution by reverse translation
US20020031762A1 (en) * 1998-04-17 2002-03-14 Charles Everett Merryman Method for producing diverse libraries of encoded polypeptides

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6429300B1 (en) * 1999-07-27 2002-08-06 Phylos, Inc. Peptide acceptor ligation methods
DE60129809T2 (en) * 2000-05-19 2008-04-30 Richard B. South Pasadena Williams IN VITRO EVOLUTION OF NUCLEIC ACIDS AND CODED POLYPEPTIDES
WO2003044194A1 (en) * 2001-11-16 2003-05-30 Proteonova, Inc. Selection and evolution of nucleic acids and polypeptides

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5843701A (en) * 1990-08-02 1998-12-01 Nexstar Pharmaceticals, Inc. Systematic polypeptide evolution by reverse translation
US5688670A (en) * 1994-09-01 1997-11-18 The General Hospital Corporation Self-modifying RNA molecules and methods of making
US20020031762A1 (en) * 1998-04-17 2002-03-14 Charles Everett Merryman Method for producing diverse libraries of encoded polypeptides

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP1692155A4 *

Also Published As

Publication number Publication date
WO2005072087A2 (en) 2005-08-11
EP1692155A4 (en) 2008-08-06
JP2007513631A (en) 2007-05-31
AU2004314705A1 (en) 2005-08-11
CA2548465A1 (en) 2005-08-11
EP1692155A2 (en) 2006-08-23

Similar Documents

Publication Publication Date Title
US11340232B2 (en) Peptide constructs and assay systems
CN110637086B (en) Method for producing complex of RNA molecule and peptide, and use thereof
JP2021501576A (en) Methods and kits using nucleic acid encoding and / or labeling
WO2007017229A3 (en) Method for carrying out the selective evolution of proteins in vitro
WO1999036516A3 (en) Method of determining the function of nucleotide sequences and the proteins they encode by transfecting the same into a host
WO2002074929A3 (en) Evolving new molecular function
WO2006053021A3 (en) Methods for engineering polypeptide variants via somatic hypermutation and polypeptides made thereby
WO2004081228A3 (en) Real time gene expression profiling
DE60313659D1 (en) BORRELIDINE DERIVATIVES AND ITS MEDICAL APPLICATION
HK1054410A1 (en) In vitro evolution of nucleic acids and encoded polypeptide
WO2004065570A3 (en) Methods of metallizing nucleic acid molecules and methods of attaching nucleic acid molecules to conductive surfaces
WO2005067648A3 (en) Multiplex spatial profiling of gene expression
WO2005072087A3 (en) System and methods for nucleic acid and polypeptide selection
ATE320484T1 (en) HISTONE DEACETYLASE-8 PROTEINS, NUCLEIC ACIDS AND METHODS OF USE
WO2005001099A8 (en) Protein expression system
EP2554681B1 (en) Constitution of tool for analyzing biomolecular interaction and analysis method using same
JP6478392B2 (en) Nucleic acid linker
WO2016134521A1 (en) Dna display and methods thereof
WO2020067550A1 (en) Compound library and method for producing compound library
JP2007267733A (en) Method for producing functional non-natural protein, and method for site-specific modification/immobilization of the protein
Li et al. Physiological assembly of functionally active 30S ribosomal subunits from in vitro synthesized parts
WO2022138892A1 (en) Method for screening for candidate molecule capable of forming complex in conjunction with plurality of target molecules
CN116516494A (en) Preparation method of cell-free nano antibody library
WO2002063009A8 (en) Lp mammalian proteins; related reagents
WO2001079485A3 (en) Direct isolation of transposon insertions tagging transcribed portions of genes

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BW BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE EG ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NA NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GM KE LS MW MZ NA SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LT LU MC NL PL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

WWE Wipo information: entry into national phase

Ref document number: 11415844

Country of ref document: US

WWE Wipo information: entry into national phase

Ref document number: 2004821258

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2548465

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 2006544008

Country of ref document: JP

WWE Wipo information: entry into national phase

Ref document number: 2004314705

Country of ref document: AU

ENP Entry into the national phase

Ref document number: 2004314705

Country of ref document: AU

Date of ref document: 20041210

Kind code of ref document: A

WWP Wipo information: published in national office

Ref document number: 2004314705

Country of ref document: AU

WWP Wipo information: published in national office

Ref document number: 2004821258

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 11415844

Country of ref document: US