WO2005067648A3 - Multiplex spatial profiling of gene expression - Google Patents

Multiplex spatial profiling of gene expression Download PDF

Info

Publication number
WO2005067648A3
WO2005067648A3 PCT/US2005/000516 US2005000516W WO2005067648A3 WO 2005067648 A3 WO2005067648 A3 WO 2005067648A3 US 2005000516 W US2005000516 W US 2005000516W WO 2005067648 A3 WO2005067648 A3 WO 2005067648A3
Authority
WO
WIPO (PCT)
Prior art keywords
gene expression
mass tag
spatial profiling
biological molecules
binding structure
Prior art date
Application number
PCT/US2005/000516
Other languages
French (fr)
Other versions
WO2005067648A2 (en
Inventor
Shawn Levy
Original Assignee
Univ Vanderbilt
Shawn Levy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Vanderbilt, Shawn Levy filed Critical Univ Vanderbilt
Publication of WO2005067648A2 publication Critical patent/WO2005067648A2/en
Publication of WO2005067648A3 publication Critical patent/WO2005067648A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention provides mass tag complexes that permit simultaneously obtaining information of a plurality of biological molecules. The biological molecules may be RNA or protein, and the information includes both level of expression as well as spatial disposition within a cell or tissue. The mass tag comprise a core structure, a target binding structure (e.g., nucleic acid or peptide binding structure), a cleavable linker and a mass tag that exhibits a unique mass spectroscopy signal.
PCT/US2005/000516 2004-01-08 2005-01-07 Multiplex spatial profiling of gene expression WO2005067648A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US52507504P 2004-01-08 2004-01-08
US60/535,075 2004-01-08

Publications (2)

Publication Number Publication Date
WO2005067648A2 WO2005067648A2 (en) 2005-07-28
WO2005067648A3 true WO2005067648A3 (en) 2005-10-13

Family

ID=34794203

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/000516 WO2005067648A2 (en) 2004-01-08 2005-01-07 Multiplex spatial profiling of gene expression

Country Status (1)

Country Link
WO (1) WO2005067648A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1891426B1 (en) 2005-06-07 2011-10-12 Centre National De La Recherche Scientifique -Cnrs- Use of ionic matrices for maldi mass spectrometry analysis of tissue sections
EP1957983A4 (en) * 2005-11-21 2010-03-24 Univ Columbia Multiplex digital immuno-sensing using a library of photocleavable mass tags
GB0811574D0 (en) * 2008-06-24 2008-07-30 Trillion Genomics Ltd Characterising planar samples by mass spectrometry
PL2556171T3 (en) 2010-04-05 2016-04-29 Prognosys Biosciences Inc Spatially encoded biological assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
US9868979B2 (en) 2013-06-25 2018-01-16 Prognosys Biosciences, Inc. Spatially encoded biological assays using a microfluidic device
ES2955916T3 (en) 2015-04-10 2023-12-11 Spatial Transcriptomics Ab Multiplex analysis of biological specimens of spatially distinguished nucleic acids

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
GYGI S.P. ET AL: "Quantitative analysis of complex protein mixtures using isotope-coded affinity tags.", NATURE BIOTECHNOLOGY., vol. 17, October 1999 (1999-10-01), pages 994 - 999, XP002226427 *

Also Published As

Publication number Publication date
WO2005067648A2 (en) 2005-07-28

Similar Documents

Publication Publication Date Title
WO2005067648A3 (en) Multiplex spatial profiling of gene expression
WO2006031800A3 (en) Reverse transfection of cell arrays
WO2005007814A3 (en) Genome mapping of functional dna elements and cellular proteins
WO2004024757A3 (en) Modified pna molecules
WO2007053245A3 (en) Methods and composition to generate unique sequence dna probes, iabeling of dna probes and the use of these probes
WO2007039319A8 (en) Dna microarray for rapid identification of candida albicans in blood cultures
WO2006131928A8 (en) Novel nucleotide and amino acid sequences, and assays and methods of use thereof for diagnosis
WO2003016475A3 (en) Nucleic acid and amino acid sequences involved in pain
WO2007123744A3 (en) Systems and devices for sequence by synthesis analysis
WO2005097205A3 (en) Dna virus microrna and methods for inhibiting same
WO2003062462A3 (en) Method for isolating nucleic acids and protein from a single sample
WO2001040798A3 (en) Methods of using randomized libraries of zinc finger proteins for the identification of gene function
WO2005042716A3 (en) Nucleic acid binding oligonucleotides
WO2003064605A3 (en) Crude biological derivatives competent for nucleic acid detection
WO2007039903A3 (en) Novel nucleotide and amino acid sequences, and assays and methods of use thereof for diagnosis
WO2007136736A3 (en) Methods for nucleic acid sorting and synthesis
WO2003025154A3 (en) Methods and compositions for the construction and use of fusion libraries
WO2001084148A3 (en) Pharmacogenomics and identification of drug targets by reconstruction of signal transduction pathways based on sequences of accessible regions
WO2006005718A3 (en) Novel sequence for improving expression of nucleic acid
WO2002102825A3 (en) Fluorescently labelled locked nucleic acids
WO2009012363A3 (en) Nucleotides and aptamers containing boronic acid groups having biased binding to glycosylated proteins, and uses thereof
WO2004087965A3 (en) Statistical analysis of regulatory factor binding sites of differentially expressed genes
WO2006054297A3 (en) Novel nucleotide and amino acid sequences, and assays and methods of use thereof for diagnosis
AU2003216184A1 (en) Nucleic acid molecules encoding cd1-derived endosomal targeting proteins and uses thereof
WO2006076628A3 (en) Compositions comprising promoter sequences and methods of use

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BW BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE EG ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NA NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GM KE LS MW MZ NA SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LT LU MC NL PL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

NENP Non-entry into the national phase

Ref country code: DE

WWW Wipo information: withdrawn in national office

Country of ref document: DE

122 Ep: pct application non-entry in european phase