WO2005071090A8 - Two-component rna virus-derived plant expression system - Google Patents

Two-component rna virus-derived plant expression system

Info

Publication number
WO2005071090A8
WO2005071090A8 PCT/EP2005/000492 EP2005000492W WO2005071090A8 WO 2005071090 A8 WO2005071090 A8 WO 2005071090A8 EP 2005000492 W EP2005000492 W EP 2005000492W WO 2005071090 A8 WO2005071090 A8 WO 2005071090A8
Authority
WO
WIPO (PCT)
Prior art keywords
replicon
plant
replicating
rna virus
expression system
Prior art date
Application number
PCT/EP2005/000492
Other languages
French (fr)
Other versions
WO2005071090A1 (en
Inventor
Sylvestre Marillonet
Carola Thoeringer
Victor Klimyuk
Yuri Gleba
Original Assignee
Icon Genetics Ag
Sylvestre Marillonet
Carola Thoeringer
Victor Klimyuk
Yuri Gleba
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Icon Genetics Ag, Sylvestre Marillonet, Carola Thoeringer, Victor Klimyuk, Yuri Gleba filed Critical Icon Genetics Ag
Priority to EP05701045A priority Critical patent/EP1706497B1/en
Priority to AU2005206283A priority patent/AU2005206283B2/en
Priority to JP2006550024A priority patent/JP2007518412A/en
Priority to US10/586,998 priority patent/US8597950B2/en
Priority to DE602005023581T priority patent/DE602005023581D1/en
Priority to CA002554214A priority patent/CA2554214A1/en
Priority to DK05701045.6T priority patent/DK1706497T3/en
Priority to AT05701045T priority patent/ATE481494T1/en
Publication of WO2005071090A1 publication Critical patent/WO2005071090A1/en
Publication of WO2005071090A8 publication Critical patent/WO2005071090A8/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells

Abstract

A process for replicating or for replicating and expressing a sequence of interest in a plant, comprising: (i) an RNA replicon or a precursor thereof, said RNA replicon being derived from a plus-sense single stranded RNA virus and comprising at least one sequence of interest; and (ii) a helper replicon, or a precursor thereof, wherein said helper replicon is (a) incapable of systemic movement in said plant both in the presence and in the absence of said RNA replicon (i) and (b) capable of expressing in a plant one or more proteins necessary for systemic movement of said RNA replicon (i), whereby said RNA replicon (i) is capable of replicating or replicating and expressing said sequence of interest in said plant, but unable to move systemically in said plant in the absence of said one or more proteins expressed by said helper replicon (ii).
PCT/EP2005/000492 2004-01-23 2005-01-19 Two-component rna virus-derived plant expression system WO2005071090A1 (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
EP05701045A EP1706497B1 (en) 2004-01-23 2005-01-19 Two-component rna virus- derived plant expression system
AU2005206283A AU2005206283B2 (en) 2004-01-23 2005-01-19 Two-component RNA virus-derived plant expression system
JP2006550024A JP2007518412A (en) 2004-01-23 2005-01-19 Two-component RNA virus-derived plant expression system
US10/586,998 US8597950B2 (en) 2004-01-23 2005-01-19 Two-component RNA virus-derived plant expression system
DE602005023581T DE602005023581D1 (en) 2004-01-23 2005-01-19 DERIVED FROM RNA VIRUSES FROM A PLANT EXPRESSION SYSTEM COMPRISING TWO COMPONENTS
CA002554214A CA2554214A1 (en) 2004-01-23 2005-01-19 Two-component rna virus-derived plant expression system
DK05701045.6T DK1706497T3 (en) 2004-01-23 2005-01-19 Two-component system for expression of plants derived from RNA viruses
AT05701045T ATE481494T1 (en) 2004-01-23 2005-01-19 TWO-COMPONENT PLANT EXPRESSION SYSTEM DERIVED FROM RNA VIRUSES

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP04001460A EP1564295A1 (en) 2004-01-23 2004-01-23 RNA virus-derived plant expression system
EP04001460.7 2004-01-23

Publications (2)

Publication Number Publication Date
WO2005071090A1 WO2005071090A1 (en) 2005-08-04
WO2005071090A8 true WO2005071090A8 (en) 2006-01-19

Family

ID=34684645

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2005/000492 WO2005071090A1 (en) 2004-01-23 2005-01-19 Two-component rna virus-derived plant expression system

Country Status (10)

Country Link
US (1) US8597950B2 (en)
EP (2) EP1564295A1 (en)
JP (1) JP2007518412A (en)
AT (1) ATE481494T1 (en)
AU (1) AU2005206283B2 (en)
CA (1) CA2554214A1 (en)
DE (1) DE602005023581D1 (en)
DK (1) DK1706497T3 (en)
ES (1) ES2350187T3 (en)
WO (1) WO2005071090A1 (en)

Families Citing this family (21)

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US7683238B2 (en) 2002-11-12 2010-03-23 iBio, Inc. and Fraunhofer USA, Inc. Production of pharmaceutically active proteins in sprouted seedlings
US7692063B2 (en) 2002-11-12 2010-04-06 Ibio, Inc. Production of foreign nucleic acids and polypeptides in sprout systems
ES2531125T3 (en) 2003-02-03 2015-03-10 Ibio Inc System for gene expression in plants
ES2532609T3 (en) * 2004-02-20 2015-03-30 Ibio, Inc. Systems and methods for clonal expression in plants
EP1616959A1 (en) * 2004-07-07 2006-01-18 Icon Genetics AG Biological safe transient protein expression in plants
MX2008015182A (en) 2006-05-29 2008-12-12 Icon Genetics Gmbh Plant virus-based inducible expression system.
EP2593553B1 (en) 2010-07-16 2018-03-14 Philip Morris Products S.A. Methods for producing proteins in plants
CA2805799A1 (en) * 2010-07-19 2012-01-26 The Regents Of The University Of California Plant-based production of heterologous proteins
EP2418283A1 (en) 2010-08-07 2012-02-15 Nomad Bioscience GmbH Process of transfecting plants
EP2584042A1 (en) 2011-10-17 2013-04-24 Nomad Bioscience GmbH Production, storage and use of cell wall-degrading enzymes
EP2647715A1 (en) 2012-04-03 2013-10-09 Nomad Bioscience GmbH Agrobacterium for transient transfection of whole plants
JP6453315B2 (en) 2013-05-23 2019-01-16 ノマド・バイオサイエンス・ゲーエムベーハー Methods to bring abiotic stress resistance to plants
EP3097783B1 (en) 2015-05-26 2019-11-13 Nomad Bioscience GmbH Colicin m for the control of ehec
EP3178313A1 (en) 2015-12-09 2017-06-14 Leibniz-Institut für Pflanzenbiochemie Gene and protein for the synthesis of oxidized zingiberene derivatives
EP3372091A1 (en) 2017-03-07 2018-09-12 Nomad Bioscience GmbH Method of reducing contamination of an object with clostridium
EP3378485A1 (en) 2017-03-24 2018-09-26 Nomad Bioscience GmbH Bacteriocins for control of salmonella enterica
EP3752185A1 (en) 2018-02-15 2020-12-23 Icon Genetics GmbH Immunogenic composition and vaccine for generating an immune response to norovirus
WO2020182860A1 (en) 2019-03-12 2020-09-17 Icon Genetics Gmbh Norovirus-like particles with improved stability
US20220227818A1 (en) 2019-06-06 2022-07-21 Nomad Bioscience Gmbh Klebicins for the control of klebsilella
WO2022012926A1 (en) 2020-07-16 2022-01-20 Nomad Bioscience Gmbh Products for oral consumption with reduced sugar content
EP4248987A1 (en) 2022-03-21 2023-09-27 Nomad Bioscience GmbH Chimeric bacteriocins and method for the control of pseudomonas

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH08505289A (en) * 1992-12-30 1996-06-11 バイオソース ジェネティクス コーポレイション Cross reference for applications related to virus amplification of recombinant messenger RNA in transgenic plants
EP0991764B1 (en) * 1997-06-12 2006-07-12 Dow AgroSciences LLC Regulatory sequences for transgenic plants
EP1025234A1 (en) * 1997-10-24 2000-08-09 E.I. Du Pont De Nemours And Company Binary viral expression system in plants
US6632980B1 (en) 1997-10-24 2003-10-14 E. I. Du Pont De Nemours And Company Binary viral expression system in plants
WO2000053780A2 (en) * 1999-03-09 2000-09-14 Large Scale Biology Corporation Multiple component rna vector system for expression of foreign sequences
DE10109354A1 (en) * 2001-02-27 2002-09-05 Icon Genetics Ag Recombinant viral switch systems
DE10121283B4 (en) * 2001-04-30 2011-08-11 Icon Genetics GmbH, 80333 Methods and vectors for amplification or expression of desired nucleic acid sequences in plants
ATE506436T1 (en) * 2002-04-30 2011-05-15 Icon Genetics Gmbh TRANS-SPLICING BASED AMPLIFICATION VECTORS
MXPA06003715A (en) 2003-11-10 2006-06-23 Icon Genetics Ag Rna virus-derived plant expression system.
DE602004027403D1 (en) * 2003-11-10 2010-07-08 Icon Genetics Ag DERIVED FROM RNA VIRUS PLANT EXPRESSION SYSTEM

Also Published As

Publication number Publication date
EP1706497A1 (en) 2006-10-04
ES2350187T3 (en) 2011-01-19
US20070300330A1 (en) 2007-12-27
WO2005071090A1 (en) 2005-08-04
CA2554214A1 (en) 2005-08-04
AU2005206283A1 (en) 2005-08-04
EP1706497B1 (en) 2010-09-15
ATE481494T1 (en) 2010-10-15
AU2005206283B2 (en) 2009-09-03
DE602005023581D1 (en) 2010-10-28
EP1564295A1 (en) 2005-08-17
DK1706497T3 (en) 2010-12-20
US8597950B2 (en) 2013-12-03
JP2007518412A (en) 2007-07-12

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