WO2005070418A1 - Immunomodulatory alkaloids - Google Patents
Immunomodulatory alkaloids Download PDFInfo
- Publication number
- WO2005070418A1 WO2005070418A1 PCT/GB2005/000215 GB2005000215W WO2005070418A1 WO 2005070418 A1 WO2005070418 A1 WO 2005070418A1 GB 2005000215 W GB2005000215 W GB 2005000215W WO 2005070418 A1 WO2005070418 A1 WO 2005070418A1
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- WIPO (PCT)
- Prior art keywords
- alkaloid
- cells
- dendritic cells
- cell
- derivative
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Definitions
- the present invention relates to methods for inducing IL-2 production in dendritic cells with alkaloids, to various medical applications thereof and to various products, compositions and vaccines based thereon.
- the immune system When the immune system is challenged by a foreign antigen it responds by launching a protective response. This response is characterized by the coordinated interaction of both the innate and acquired immune systems. These systems, once thought to be separate and independent, are now recognized as two interdependent parts that when integrated fulfil two mutually exclusive requirements: speed (contributed by the innate system) and specificity (contributed by the adaptive system).
- the innate immune system serves as the first line of defence against invading pathogens, holding the pathogen in check while the adaptive responses are matured. It is triggered within minutes of infection in an antigen- independent fashion, responding to broadly conserved patterns in the pathogens (though it is not non-specific, . and can. istinguish between self and pathogens).
- the adaptive response becomes effective over days or weeks, but ultimately provides the fine antigenic specificity required for complete elimination of the pathogen and the generation of immunologic memory. It is mediated principally by T and B cells that have undergone germline gene rearrangement and are characterized by anaki specificity and long-lasting memory.
- the activated DCs then migrate to lymph nodes. Once there, they activate immune cells of the adaptive response (principally na ⁇ ve B- and T-cells) by acting as antigen-presenting cells (APCs). The activated cells then migrate to the sites of infection (guided by the "danger signal") and once there further amplify the response by recruiting cells of the innate immune system (including eosinophils, basophils, monocytes, NK cells and granulocytes).
- APCs antigen-presenting cells
- the adaptive immune response is principally effected via two independent limbs: cell-mediated (type 1 ) immunity and antibody-mediated or humoral (type 2) immunity.
- Type 1 immunity involves the activation of T-lymphocytes that either act upon infected cells bearing foreign antigens or stimulate other cells to act upon infected cells. This branch of the immune system therefore effectively contains and kills cells that are cancerous or infected with pathogens (particularly viruses).
- Type 2 immunity involves the generation of antibodies to foreign antigens by B-lymphocytes. This antibody-mediated branch of the immune system attacks and effectively ' neutralizes extracellular foreign antigens.
- Both limbs of the immune system are important in fighting disease and there is an increasing realization that the type of immune response is just as important as its intensity or its duration.
- the balance of the typel/type 2 response also referred to as the Th1:Th2 response ratio/balance by reference to the distinct cytokine and effector cell subsets involved in the regulation of each response - see below
- the immune response is skewed heavily towards a type 1 or type 2 response soon after exposure to antigen.
- the mechanism of this typel/type 2 skewing or polarization is not yet fully understood, but is known to involve a complex system of cell-mediated chemical messengers (cytokines, and particularly chemokines) in which the typel/type 2 polarization (or balance) is determined, at least in part, by the nature of the initial PRR-PAMP interaction when the DCs and macrophages of the innate immune system are first stimulated and subsequently by the cytokine milieu in which antigen priming of na ⁇ ve helper T cells occurs.
- cytokines cell-mediated chemical messengers
- chemokines cell-mediated chemical messengers
- cytokines Two cytokines in particular appear to have early roles in determining the path of the immune response.
- Interleukin-12 (IL-12), secreted by macrophages, drives the type 1 response by stimulating the differentiation of Th1 cells, the helper cells that oversee the type 1 response.
- Another macrophage cytokine (IL- 10) inhibits this response, instead driving a type 2 response.
- the type 1 and type 2 responses can be distinguished inter alia on the basis of certain phenotypic changes attendant on priming and subsequent polarization of na ⁇ ve helper T cells. These phenotypic changes are characterized, at least in part, by the nature of the cytokines secreted by the polarized helper T cells.
- Th1 cells produce or are regulated by so-called TM cytokines, which include one or more of TNF, IL- , IL-2, IFN-gamma, IL-12 and/or IL-18.
- the Th1 cytokines are involved in macrophage activation and Th1 cells orchestrate Type 1 responses.
- Th2 cells produce so-called Th2 cytokines, which include one or more of IL-4, IL-5, IL-10 and IL-13.
- the Th2 cytokines promote the production of various antibodies and can . suppress the type 1 response.
- Th1 response and Th2 response are used to define the type 1 and type 2 immune responses, respectively.
- Th1 response and Th2 response are used interchangeably herein.
- the type of immune response is just as important in therapy and prophylaxis as its intensity or its duration.
- Th1 response can result in autoimmune disease, inappropriate inflammatory responses and transplant rejection.
- Th2 response can lead to allergies and asthma.
- a perturbation in the Th1 :Th2 ratio is symptomatic of many immunological diseases and disorders, and the development of methods for altering the Th1 :Th2 ratio is now a priority.
- alkaloid is used herein sensh st ' ricto to define any basic, organic, nitrogenous compound which occurs naturally in an organism.
- alkaloid is also used herein sensu lato to define a broader grouping of compounds which include not only the naturally occurring alkaloids, but also their synthetic and semi-synthetic analogues and derivatives.
- the term alkaloid covers not only naturally-occuring basic, organic, nitrogenous compounds but also derivatives and analogues thereof which are not naturally occurring and which may be neither basic nor nitrogenous.
- alkaloids are phytochemicals, present as secondary metabolites in plant tissues (where they may play a role in defence), but some occur as secondary metabolites in the tissues of animals, microorganisms and fungi.
- the standard techniques for screening microbial cultures are inappropriate for detecting many classes of alkaloids (particularly highly polar alkaloids, see below) and that microbes (including bacteria and fungi, particularly the filamentous representatives) will prove to be an important source of alkaloids as screening techniques become more sophisticated.
- alkaloids exhibit great diversity. Many alkaloids are small molecules, with molecular weights below 250 Daltons. The skeletons may be derived from amino acids, though some are derived from other groups (such as steroids). Others can be considered as sugar analogues. It is becoming apparent (see Watson ef al. (2001) Phytochemistry 56: 265-295) that the watqr soluble fractions of medicinal plants and microbial cultures contain many interesting novel polar alkaloids, including many carbohydrate analogues. Such analogues include a rapidly growing number of polyhydroxylated alkaloids. Most alkaloids are classified structurally on the basis of the configuration of the N-heterocycle.
- alkaloids are pharmacologically active, and humans have been using alkaloids (typically in the form of plant extracts) as poisons, narcotics, stimulants and medicines for thousands of years.
- alkaloids typically in the form of plant extracts
- the therapeutic applications of polyhydroxylated alkaloids have been comprehensively reviewed in Watson et al. (2001), ibidem: applications include cancer therapy, immune stimulation, the treatment of , diabetes, the treatment of infections (especially viral infections), therapy of glycosphingolipid lysosomal storage diseases and the treatment of autoimmune disorders (such as arthritis and sclerosis).
- Alexine (1) and australine (2) were the first alkaloids to be isolated with a carbon substituent at C-3, rather than the more common C-1 substituents characteristic of the necine family of pyrrolizidines.
- Australine (2) The alexines occur in all species of the genus Alexa and also in the related species Castanospermum australe. Stereoisomers of alexine, including 1 ,7a-diepialexine (3), have also been isolated (Nash et al. (1990) Phytochemistry (29) 111 ) and synthesised (Choi ef a/. (1991) Tetrahedron Letters (32) 5517 and Denmark and Cottell (2001) J. Org. Chem. (66) 4276-4284).
- swainsonine (4) is a potent and specific inhibitor of ⁇ -mannosidase and is reported to have potential as an antimetastic, tumour anti-proliferative and immunoregulatory agent (see e.g. US5650413, WO00/37465, WO93/09117).
- indolizidine alkaloid castanospermine (5)
- castanospermine (5) is a potent ⁇ -glucosidase inhibitor.
- This compound along with certain 6-O-acyl derivatives (such as that known as Bucast (6)), has been reported to exhibit anti-viral and antimetastic activities.
- Casuarine, (1 R,2R,3R,6S,7S,7aR)-3-(hydroxymethyl)-1 ,2,6,7-tetrahydroxy(10) is a highly oxygenated bicyclic alkaloid that can be regarded as a more highly oxygenated analogue of the 1 ,7a-diepialexine (shown in 3) or as a C(3) hydroxy methyl-substituted analogue of the 1 ⁇ ,2 ⁇ ,6 ⁇ ,7 ⁇ ,7 ⁇ -1 ,2,6,7-tetrahydroxy(shown in 9).
- Casuarine can be isolated from several botanical sources, including the bark of Casuarina equisetifolia
- Casuarina equisetifolia wood, bark and leaves have been claimed to be useful against diarrhoea, dysentery and colic (Chopra et al. (1956) Glossary of Indian Medicinal Plants, Council of Scientific and Industrial Research (India), New Delhi, p. 55) and a sample of bark has recently been prescribed in Western Samoa for the treatment of breast cancer.
- An African plant containing casuarine (identified as Syzygium guineense) has been reported to be beneficial in the treatment of AIDS patients (see Wormald et al. (1996) Carbohydrate Letters (2) 169-174).
- casuarine-6- ⁇ -glucoside casuarine-6- ⁇ -D-glucopyranose, 11
- casuarine-6- ⁇ -D-glucopyranose 11
- has also been isolated from the bark and leaves of Eugenia jambolana Woodd et al. (1996) Carbohydrate Letters (2) 169-174).
- pyrrolidine alkaloids appear to be fairly widespread secondary metabolites: for example, 2R.5R- dihydroxymethyl-3R,4R-dihydroxypyrrolidine (DMDP) (12) and 1 ,4-dideoxy-1 ,4-imino-D-arabinitol (D-AB1 ) (13) have been isolated from species of both temperate and tropical plants from quite unrelated families, and DMDP is also produced by a species of the filamentous bacterium Streptomyces.
- DMDP 2R.5R- dihydroxymethyl-3R,4R-dihydroxypyrrolidine
- D-AB1 1 ,4-dideoxy-1 ,4-imino-D-arabinitol
- DMDP has been shown to have nematocidal activity: WO 92/09202 describes the use of the compound in controlling diseases caused by parasitic nematodes in both plants and mammals.
- DCs Dendritic cells
- Dendritic cells therefore act as highly specialized antigen-presenting cells (APCs): serving as "nature's adjuvants", they potentiate adaptive T-cell dependent immunity as well as triggering the natural killer (NK and NKT) cells of the innate immune system. Dendritic cells therefore play a fundamental and important regulatory role in the magnitude, quality, and memory of the immune response. As a result, there is now a growing interest in the use of dendritic cells in various immunomodulatory interventions, which are described in more detail below.
- APCs antigen-presenting cells
- Dendritic cells can be classified into different subsets inter alia on the basis of their state of maturation (mature or immature) and their cellular developmental origin (ontogeny). Each of these subsets appear to play distinct roles in vivo, as described below.
- Dendritic Cell Maturation Immature (or resting) DCs are located in non-lymphoid tissue, such as the skin and mucosae, are highly phagocytic and readily internalize soluble and particulate antigens. It is only when such antigen-loaded immature DCs are also subject to inflammatory stimuli (referred to as maturation stimuli) that they undergo a maturation process that transforms them from phagocytic and migratory cells into non-phagocytic, highly efficient stimulators of na ⁇ ve T cells.
- maturation stimuli inflammatory stimuli
- Immature DCs are characterized by high intracellular MHC II in the form of MIICs, the expression of CD1 a, active endocytosis for certain particulates and proteins, presence of FcgR and active phagocytosis, deficient T cell sensitization in vitro, low/absent adhesive and costimulatory molecules (CD40/54/58/80/86), low/absent CD25, CD83,- p55, DEC-205, 2A1 antigen, responsiveness to GM-CSF, but not M-CSF and G-CSF and a sensitivity to IL-10, which inhibits maturation.
- mature DCs Upon maturation, mature DCs, loaded with antigen and capable of priming T cells, migrate from the non- lymphoid tissues to the lymph nodes or spleen, where they process the antigen load and present it to the resident na ⁇ ve CD4 + T cells and CD8 + cytotoxic T cells. This latter interaction generates CTLs, the cellular arm of the adaptive immune response, and these cells eliminate virally infected cells and tumour cells.
- the na ⁇ ve CD4 + T cells differentiate into memory helper T cells, which support the differentiation and expansion of CD8 + CTLs and B cells.
- helper T cells exert anti-tumour activity indirectly through the activation of important effector cells such as macrophages and CTLs. Having activated the T cells in this way, the mature DCs undergo apoptosis within 9-10 days.
- Mature DC cells are characterized morphologically by motility and the presence of numerous processes (veils or dendrites). They are competent for antigen capture and presentation (exhibiting high MHC class I and II expression) and express a wide range of molecules involved in T cell binding and costimulation, (e.g. CD40, CD54/1CAM-1 , CD58/LFA-3, CD80/B7-1 and CD86/B7-2) as well as various cytokines (including IL-12). They are phenotypically stable: there is no reversion/conversion to macrophages or lymphocytes.
- mature DCs play an important role in T cell activation and cell-mediated immunity.
- immature DCs are involved in regulating and maintaining immunologica! tolerance (inducing antigen-specific T cell anergy).
- Dendritic cells are not represented by a single cell type, but rather comprise a heterogeneous collection of different classes of cells, each with a distinct ontogeny. At least three different developmental pathways have been described, each emerging from unique progenitors and driven by particular cytokine combinations to DC subsets with distinct and specialized functions.
- the primitive CD34 + DC progenitors are subject to various stimulatory signals. These signals can direct the progenitors along one of at least three different pathways, each differing with respect to intermediate stages, cytokine requirements, surface marker expression and biological function.
- Lymphoid DCs are a distinct subset of DCs that are closely linked to the lymphocyte lineage. This lineage is characterized by the lack of the surface antigens CD11 b, CD13, CD14 and CD33. Lymphoid DCs share ancestry with T and natural killer (NK) cells, the progenitors for all being located in the thymus and in the T cell areas of secondary lymphoid tissues. The differentiation of lymphoid DCs is driven by interleukins 2, 3 and 15 (IL-3, IL-2 and IL-15), but not by granulocyte macrophage colony- stimulating factor (GM-CSF).
- IL-3, IL-2 and IL-15 granulocyte macrophage colony- stimulating factor
- lymphoid promote negative selection in the thymus (possibly by inducing fas-mediated apoptosis) and are costimulatory for CD4 + and CD8 + T cells. More recently, lymphoid-like DCs derived from human progenitors have also been shown to preferentially activate the Th2 response. Because of their capacity to induce apoptosis and their role in eliminating potentially self-reactive T cells, it has been suggested that lymphoid DCs primarily mediate regulatory rather than stimulatory immune effector functions.
- Myeloid DCs are distinguished by a development stage in which there is expression of certain features associated with phagocytes. There appear to be at least two structurally and functionally distinct subsets. The first is defined antigenically as CD14 " , CD34 + , CD68 " and CD1a + and sometimes referred to as DCs of the Langerhans cell type. This subset appears to prime T cells to preferentially activate Th1 responses and IL-12 appears implicated in this process. The subset may also activate na ⁇ ve B cells to secrete IgM and may therefore be predominantly associated with an inflammatory Th1 response.
- a second myeloid DC subset is defined antigenically as CD14 + , CD68 + and CD1a " and related to monocytes (as a result they are also referred to as monocyte-derived DCs or Mo-DCs).
- DC cells are taken from a patient (for example by apheresis) and then loaded (pulsed, primed or spiked) with a particular antigen or antigens (for example, tumour antigen(s)). They are then re-administered as an autologous cellular vaccine to potentiate an appropriate immune response.
- a particular antigen or antigens for example, tumour antigen(s)
- the responding T cells include helper cells, especially Th1 CD4 + cells (which . produce IFN- ⁇ ) and killer cells (especially CD8 + cytolytic T lymphocytes).
- helper cells especially Th1 CD4 + cells (which . produce IFN- ⁇ ) and killer cells (especially CD8 + cytolytic T lymphocytes).
- the DCs may also mediate responses by other classes of lymphocytes (B, NK, and NKT cells). They may also elicit T cell memory, a critical goal of vaccination.
- Mo-DCs These cells are obtained by exposing monocytes to GM-CSF and IL-4 (or IL-13) to produce immature Mo-DCs, which are then matured by incubation in a maturation medium.
- Such media comprise one or more maturation stimulation factor(s), and typically comprise C-type lectins, Toll-like receptor (TLR) ligands (e.g. microbial products such as lipopolysaccharide and/or monophosphoryl lipid), inflammatory cytokines (such as TNF- ⁇ ), CD40L, monocyte conditioned medium (MCM) or MCM mimic (which contains IL-1 ⁇ , TNF- ⁇ , IL-6 and PGE 2 ).
- TLR Toll-like receptor
- MCM or MCM mimic currently represent a standard: Mo-DCs matured using these media are homogenous, have a high viability, migrate well to chemotactic stimuli and induce CTLs both in vitro and in vivo.
- Dendritic cells for vaccination have also been prepared from CD34 + -derived DCs comprising a mixture of interstitial and DCs of the Langerhans cell type. Some workers believe that the latter DC subset are more potent than Mo-DCs when used as immunostimulatory DC vaccines.
- antigen selection various approaches have been used. Both defined and undefined antigens can be employed.
- the antigens can be xenoantigens or autoantigens.
- One or more defined neoantigen(s) may be selected: in the case of cancer treatment, the neoantigen(s) may comprise a tumour-associated antigen.
- most popular are 9-11 amino acid peptides containing defined antigens " (either natural sequences or analogues designed for enhanced MHC binding): such antigens can be manufactured to good manufacturing practice (GMP) standard and are easily standardized.
- GMP manufacturing practice
- Antigens can also be loaded by transfecting the DCs with encoding nucleic acid (e.g. by electroporation) such that the antigens are expressed by the DC, processed and presented at the cell surface.
- This approach avoids the need for expensive GMP proteins and antibodies.
- RNA is preferred for this purpose, since it produces only transient expression (albeit sufficient for antigen processing) and avoids the potential problems associated with the integration of DNA and attendant long-term expression/mutagenesis.
- Such transfection techniques also permit exploration of the whole antigenic repertoire of a target cell by use of total or PCR-amplified tumour RNA.
- helper proteins for example, keyhole limpet hemocyanin (KLH) and tetanus toxoid (TT)
- KLH keyhole limpet hemocyanin
- TT tetanus toxoid
- the dose, frequency and route of DC vaccine administration have not yet been optimised in clinical trials.
- the absolute number of cells administered will depend on the route of administration and effectiveness of migration after infusion.
- DCs are targeted to a tumour and activated to elicit immune responses in situ without the need for ex vivo antigen loading.
- In situ DC vaccination constitutes yet another distinct (but related) approach (Hawiger et al. (2001) J Exp Med 194: 769-779.
- antigen is targeted to DCs in vivo which are then expanded and induced to mature in situ.
- This approach involves targeting of antigen to endogenous DCs (for example, using exosomes - see Thery ef al. (2002) Nat Rev Immunol 2: 569-579) and the development of maturation stimulants that can effectively trigger maturation (preferably of defined DC subset(s)) in vivo.
- Cytotoxic T lymphocytes can be administered to a patient in order to confer or supplement an immune response to a particular disease or infection (typically cancer).
- a particular disease or infection typically cancer
- tumour specific T cells can be extracted from a patient (e.g. by leukapheresis), selectively expanded (for example by tetramer-guided cloning - see Dunbar ef al. (1999) J Immunol 162: 6959-6962) and then re-administered as an autologous cellular vaccine.
- Dendritic cells are also involved in regulating and maintaining immunological tolerance: in the absence of maturation, the cells induce antigen-specific silencing or tolerance.
- immature DCs are administered as part of an immunomodulatory intervention designed to combat autoimmune disorders.
- the suppressive potential of the DCs can be enhanced by in vitro transfection with genes encoding cytokines.
- Granucci describes the important role played by DC-derived IL-2 in mediating not only T cell activation but also that of NK cells and goes on to suggest that DC-derived IL-2 is a key factor regulating and linking innate and adaptive immunity.
- the immune response comprises two distinct types: the Th1 response (type-1 , cellular or cell mediated immunity) and Th2 response (type-2, humoral or antibody mediated immunity).
- Th1 and Th2 responses are not mutually exclusive and in many circumstances they occur in parallel. In such circumstances the balance of the Th1/Th2 response determines the nature (and repercussions) of the immunological defence (as explained below).
- the Th1 Th2 balance (which can be expressed as the Th1 :Th2 response ratio) is determined, at least in part, by the nature of the environment (and in particular the cytokine milieu) in which antigen priming of na ⁇ ve helper T cells occurs when the immune system is first stimulated.
- Th1 and Th2 responses are distinguished inter alia on the basis of certain phenotypic changes attendant on priming and subsequent polarization of na ⁇ ve helper T cells. These phenotypic changes are characterized, at least in part, by the nature of the cytokines secreted by the polarized helper T cells.
- Th1 cells produce or are regulated by so-called Th1 cytokines, which include one or more of TNF, IL-1 , IL-2, IFN-gamma, IL-12 and/or IL-18.
- the Th1 cytokines orchestrate the type 1 response and are involved in macrophage activation and Th1 cells orchestrate cell-mediated defences (including cytotoxic T lymphocyte production) that form a key limb of the defence against bacterial and viral attack, as well as malignant cells.
- Th2 cells produce so-called Th2 cytokines, which include one or more of IL-4, IL-5, IL-10 and 1L-13.
- the Th2 cytokines promote the production of various antibodies and can suppress the Th1 response.
- Th1 a cell that makes IFN-gamma and not IL-4
- Th2 a CD4 + cell that expresses IL-4 and not IFN-gamma
- Th1 or Th2 the phenotype of the T cell response
- Th1 :Th2 ratio is symptomatic of many immunological diseases and disorders, and the development of methods for altering the Th1:Th2 ratio is now a priority. This need is also addressed by the present invention.
- the present invention is based, at least in part, on the surprising discovery that IL-2 production by dendritic cells can be induced by certain alkaloids.
- the IL-2 production is significant and sustained.
- the alkaloids may also induce IL-12 production by dendritic cells.
- This hitherto unsuspected activity of alkaloids has far-reaching applications in the field of immunotherapy, in particular in the areas of dendritic cell vaccines and in immunomodulatory interventions designed to increase the Th1:Th2 response ratio in vivo (for example, by preferentially promoting a Th1 response and/or preferentially suppressing a Th2 response).
- a method for inducing the production of IL-2 (and optionally IL-12) in dendritic cells in a patient in need thereof comprising administering an alkaloid to the patient at a dose sufficient to induce said IL-2 (and optionally IL-12) production in said dendritic cells.
- the invention contemplates the use of an alkaloid for the manufacture of a medicament for use in immunotherapy, wherein the immunotherapy comprises the induction of IL-2 (and optionally IL-12) production in dendritic cells.
- the immunotherapy preferably comprises:
- Th1-related diseases or disorders e.g. proliferative disorders or infection
- Th2-related diseases or disorders for example allergies, e.g. asthma
- Haemorestoration e.g. Haemorestoration
- Alleviation of immunosuppression e.g. Cytokine stimulation
- Vaccination wherein the alkaloid acts as an adjuvant
- Vaccination wherein the alkaloid acts to potentiate dendritic cells in situ
- Wound healing e
- Boosting the activity of endogenous NK cells for example in the treatment of Th1-related diseases or disorders (e.g. proliferative disorders or infection) and/or Th2-related diseases or disorders (for example allergies, e.g. asthma);
- Haemorestoration e.g. Haemorestoration
- Alleviation of immunosuppression e.g. a vacciniasis
- Cytokine stimulation e
- Treatment of proliferative disorders e
- the immunotherapy may comprise immunostimulation in the treatment or prophylaxis of a microbial infection selected from:
- immunot erapeutic interventions which comprise the treatment or prophylaxis of an infection in which the infecting pathogen resides intracellularly or causes the expression of neoantigen(s) in host cells, for example selected from: HIV, leishmania, influenza, tuberculosis and malaria.
- the haemorestoration is preferably adjunctive to: (a) chemotherapy; and/or (b) radiotherapy; and/or (c) bone marrow transplantation; and/or (d) haemoablative immunotherapy.
- the immunosuppression treated according to the invention may arise from any cause, and may be congenital, acquired (e.g. by infection or malignancy) or induced (e.g. deliberately as part of the management of transplants or cancers).
- the alkaloids of the invention may be used to stimulate the production of endogenous cytokines alone, or may be used to this end as adjuncts in a gene therapy programme (for example, one based upon the administration of nucleic acid encoding one or more cytokines, such as IL-2).
- the vaccines of the invention may be cell-based vaccine.
- Such cell-based vaccines typically comprise live cells.
- Particularly preferred are cell-based vaccines comprising dendritic cells and/or T cells.
- the vaccines of the invention may comprise a neoantigen and an alkaloid.
- the cells used according to the invention may be xenogenic, allogenic, syngenic or autogeneic cells. Preferred, however, is the use of syngenic or autogeneic cells.
- autologous cells are used (for example, removed from the patient by apheresis prior to readministration).
- the immunotherapy may further comprise the co-administration of an antigen (e.g. a neoantigen), which antigen is optionally targeted to endogenous dendritic cells (e.g. present in an exosome) and/or the co- administration of a dendritic cell maturation stimulant.
- an antigen e.g. a neoantigen
- endogenous dendritic cells e.g. present in an exosome
- a dendritic cell maturation stimulant e.g. a dendritic cell maturation stimulant.
- the invention contemplates a live cell vaccine comprising an alkaloid.
- the cells in the live cell vaccines of the invention preferably comprise xenogenic, allogenic, syngenic or autogeneic cells.
- live cell vaccines in which the cells comprise dendritic cells (for example antigen-pulsed dendritic cells).
- the live cell vaccines of the invention may also comprise T cells.
- the T cells may be primed by contact with dendritic cells, for example by contact with antigen-pulsed dendritic cells.
- T cells are primed by contact with antigen-pulsed dendritic cells in the presence of the alkaloid.
- the invention contemplates a process for producing a dendritic cell vaccine comprising the step of contacting dendritic cells with an alkaloid at a concentration sufficient to induce IL-2 production in said dendritic cells.
- the process further comprises the step of loading the dendritic cells with an antigen and/or maturing the dendritic cells.
- the dendritic cells may be matured by a convenient means (for example, by relying on spontaneous maturation), but preferred is maturation by contact with a maturation medium (for example with the maturation medium of the invention). Maturation is, of course, deliberately avoided in embodiments where the induction of tolerance is required (for example in the treatment of autoimmune disorders and allergies, as described infra).
- the invention also contemplates a dendritic cell vaccine obtained (or obtainable) by the process of the invention.
- the invention also relates to a process for producing a T cell vaccine comprising the steps of: (a) providing dendritic cells; (b) contacting the dendritic cells with an alkaloid at a concentration sufficient to induce IL-2 production in said dendritic cells, thereby to produce stimulated dendritic cells; (c) providing T cells; (d) priming the T cells by contacting them with the stimulated dendritic cells of step (b).
- the process further comprises the step of loading the dendritic cells with an antigen and/or maturing the dendritic cells prior to the priming step (d).
- a T cell vaccine obtained by the process of the invention.
- the invention contemplates a method of adoptive immunotherapy comprising administering the T cell vaccine of the invention to a patient in need thereof.
- the invention provides a method for priming T cells in vitro comprising the steps of: (a) providing dendritic cells; (b) contacting the dendritic cells with an alkaloid at a concentration sufficient to induce IL-2 production in said dendritic cells, thereby to produce stimulated dendritic cells; (c) providing T cells; (d) contacting the T cells with the stimulated dendritic cells, thereby to produce primed T cells.
- the method further comprises the step of loading the dendritic cells with an antigen and/or maturing the dendritic cells prior to the priming step (d).
- the invention provides a method of adoptive immunotherapy comprising administering T cells primed according to the methods of the invention to a patient in need thereof.
- the invention provides a maturation medium for triggering the maturation of immature dendritic cells into mature dendritic cells, said medium comprising an alkaloid at a concentration sufficient to induce IL-2 production in said dendritic cells.
- the maturation medium may be defined or undefined. It may comprise one or more Toll-like receptor (TLR) ligands and/or one or more inflammatory cytokines (such as TNF- ⁇ ). Other growth factors may also be present. Particularly preferred is alkaloid-supplemented monocyte conditioned medium (MCM) or MCM mimic.
- TLR Toll-like receptor
- MCM monocyte conditioned medium
- MCM mimic alkaloid-supplemented monocyte conditioned medium
- the invention provides a dendritic cell factory comprising the maturation medium of the invention.
- the invention also contemplates a process for producing mature dendritic cells comprising the step of contacting immature dendritic cells with the maturation medium of the invention.
- the invention provides a process for producing a dendritic cell vaccine comprising the step of contacting immature dendritic cells with the maturation medium of the invention (for example in the dendritic cell factory of the invention).
- the invention also provides a method of adoptive immunotherapy comprising administering mature dendritic cells produced according to the process of the invention to a patient in need thereof. Also contemplated is a method for activating resting NK and/or NKT cells in vivo comprising the step of administering an alkaloid to a patient in need of NK and/or NKT cell activation at a dose sufficient to induce IL-2 production in endogenous dendritic cells of the patient. The invention also provides a method for potentiating vaccination with dendritic cells in a patient in need thereof comprising the co-administration of an alkaloid at a dose sufficient to induce IL-2 production in said dendritic cells.
- a method of potentiating vaccination with T cells in a patient in need thereof comprising the co- administration of an alkaloid at a dose sufficient to induce IL-2 production in endogenous dendritic cells of the patient.
- the inventionm provides a method for inducing the maturation of dendritic cells comprising the co-administration of: (a) a antigen targeted to the dendritic cells; and (b) an alkaloid at a dose sufficient to induce IL-2 production in said dendritic cells.
- the invention also provides a method for inducing tolerance in a patient in need thereof comprising the co- administration of immature dendritic cells and an alkaloid.
- the invention provides a method for potentiating vaccination with immature dendritic cells comprising the co-administration of an alkaloid at a dose sufficient to induce IL-2 production in said immature dendritic cells.
- the immature dendritic cells are preferably loaded with an antigen.
- the term co-administration is intended to cover the sequential, concurrent or separate administration of the referenced components.
- Concurrent administration therefore covers the case where the referenced components are physically mixed prior to administration.
- Sequential administration covers circumstances in which the referenced components are administered separately with some degree of temporal separation (typically from several minutes to several hours, although in some embodiments the administration of the co-administered components may be separated by a period of one or more days).
- neoantigen is used herein to define any newly expressed antigenic determinant. Neoantigens may arise upon conformational change in a protein, as newly expressed determinants (especially on the surfaces of transformed or infected cells), as the result of complex formation of one or more molecules or as the result of cleavage of a molecule with a resultant display of new antigenic determinants.
- the term neoantigen covers antigens expressed upon infection (e.g. viral infection, protozoal infection or bacterial infection), in prion-mediated diseases (e.g. BSE and CJD), an on cell transformation (cancer), in which latter case the neoantigen may be termed a tumour-associated antigen.
- tumour-associated antigen is used herein to define an antigen present in transformed (malignant or tumourous) cells which is absent (or present in lower amounts or in a different cellular compartment) in normal cells of the type from which the tumour originated.
- Oncogenic viruses can also induce expression of tumour antigens, which are often host proteins induced by the virus.
- maturation medium is used herein to define a composition (either defined or undefined) comprising one or more compounds which induce the maturation of dendritic cells from immature dendritic cells.
- the maturation medium comprises one or more Toll-like receptor (TLR) ligands and/or one or more inflammatory cytokines (such as TNF- ⁇ ).
- TLR Toll-like receptor
- Other growth factors may also be present.
- dendritic cell factory is used herein to define cultures of dendritic cells disposed within a closed (or functionally closed) multilayered vessel in which the layers intercommunicate to provide an internal surface area large enough to accommodate at least 10 6 to 10 9 dendritic cells.
- the dendritic cell factory contains a maturation medium, in order that immature dendritic cells harvested by leukapheresis and incubated within the cell factory are thereby converted into mature dendritic cells useful as the basis for dendritic cell vaccines.
- Suitable cell factories are commercially available (for example the NunclonTM ⁇ cell factory sold by NuncTM).
- closed system as applied to a cell factory, is used to define a culture vessel which is sterile and isolated from the outside environment by aseptic barrier(s) and in which all components are fully integral, being attached and/or assembled at the manufacturing site.
- functionally closed system as applied to a cell factory, is used to define a culture vessel which is assembled by the end user from sterile modular elements produced at the device manufacturing site and which uses sterile barrier(s) (e.g. 0.22 micron filters) to permit aseptic interconnection of various modules by the end user.
- sterile barrier(s) e.g. 0.22 micron filters
- adjunctive as applied to the use of the drugs of the invention in therapy
- Such adjunctive therapies may comprise the concurrent, separate or sequential administration/application of the alkaloid of the invention and the other treatment(s).
- adjunctive use of the alkaloid of the invention is reflected in the formulation of the pharmaceutical compositions of the invention.
- adjunctive use may be reflected in a specific unit dosage, or in formulations in which the alkaloid of the invention is present in admixture with the other drug(s) with which it is to be used adjunctively (or else physically associated with the other drug(s) within a single unit dose).
- adjunctive use of the alkaloid of the invention may be reflected in the composition of the pharmaceutical kits of the invention, wherein the alkaloid of the invention is co-packaged (e.g. as part of an array of unit doses) with the other drug(s) with which it is to be used adjunctively.
- adjunctive use of the alkaloid of the invention may be reflected in the content of the information and/or instructions co-packaged with the alkaloid relating to formulation and/or posology.
- polar and non-polar are to be understood as relative terms which can be applied in the characterization of solvents to indicate the degree to which they have an electric dipole moment and so display hydrophilicity (polar) or hydrophobicity (non-polar).
- solvents can be used to extract polar and non-polar phytochemicals, respectively, and the terms polar and non-polar, as applied herein to alkaloids, phytochemicals or any other moieties, are to be interpreted accordingly.
- herbal medicine is used herein to define a pharmaceutical composition in which at least one active principle is not chemically synthesized and is a phytochemical constituent of a plant. In most cases, this non- synthetic active principle is not isolated (as defined herein), but present together with other phytochemicals with which it is associated in the source plant. In some cases, however, the plant-derived bioactive principle(s) may be in a concentrated fraction or isolated (sometimes involving high degrees of purification). In many cases, however, the herbal medicine comprises a more or less crude extract, infusion or fraction of a plant or even an unprocessed whole plant (or part thereof), though in such cases the plant (or plant part) is usually at least dried and/or milled.
- bioactive principle is used herein to define a phytochemical which is necessary or sufficient for the pharmaceutical efficacy of the herbal medicament in which it is comprised.
- the bioactive principle comprises the immunostimulatory alkaloid of the invention (e.g. casuarine, casuarine glucoside or mixtures thereof).
- the term standard specification is used herein to define a characteristic, or a phytochemical profile, which is correlated with an acceptable quality of the herbal medicine.
- quality is used to define the overall fitness of the herbal medicament for its intended use, and includes the presence of one or more of the bioactive principles (at an appropriate concentration) described above or else the presence of one or more bioactive markers or a phytochemical profile which correlates with the presence of one or more of the bioactive principles (at an appropriate concentration).
- phytochemical profile is used herein to define a set of characteristics relating to different phytochemical constituents.
- the term isolated as applied to the alkaloids of the invention is used herein to indicate that the alkaloid exists in a physical milieu distinct from that in which it occurs in nature.
- the isolated material may be substantially isolated (for example purified) with respect to the complex cellular milieu in which it naturally occurs.
- the absolute level of purity is not critical and those skilled in the art can readily determine appropriate levels of purity according to the use to which the material is to be put. Preferred, however, are purity levels of 90% w/w, 99% w/w or higher.
- the isolated alkaloid forms part of a composition (for example a more or less crude extract containing many other substances) or buffer system, which may for example contain other components.
- the isolated alkaloid may be purified to essential homogeneity, for example as determined spectrophotometrically, by NMR or by chromatography (for example GC-MS).
- derivative and pharmaceutically acceptable derivative as applied to the alkaloids of the invention define alkaloids which are obtained (or obtainable) by chemical derivatization of the parent alkaloids of the invention.
- the pharmaceutically acceptable derivatives are suitable for administration to or use in contact with the tissues of humans without undue toxicity, irritation or allergic response (i.e. commensurate with a reasonable benefit/risk ratio).
- Preferred derivatives are those obtained (or obtainable) by alkylation, esterification or acylation of the parent alkaloids of the invention.
- the derivatives may be immunostimulatory per se, or may be inactive until processed in vivo. In the latter case, the derivatives of the invention act as pro- drugs.
- Particularly preferred pro-drugs are ester derivatives which are esterified at one or more of the free hydroxyls and which are activated by hydrolysis in vivo.
- the pharmaceutically acceptable derivatives of the invention retain some or al! of the immunostimulatory activity of the parent alkaloid.
- the immunostimulatory activity is increased by derivatization.
- Derivatization may also augment other biological activities of the alkaloid, for example bioavailability and/or glycosidase inhibitory activity and/or glycosidase inhibitory profile.
- derivatization may increase glycosidase inhibitory potency and/or specificity.
- pharmaceutically acceptable salt as applied to the alkaloids of the invention defines any non-toxic organic or inorganic acid addition salt of the free base compounds which are suitable for use in contact with the tissues of humans and lower animals without undue toxicity, irritation, allergic response and which are commensurate with a reasonable benefit/risk ratio. Suitable pharmaceutically acceptable salts are well known in the art.
- Examples are the salts with inorganic acids (for example hydrochloric, hydrobromic, sulphuric and phosphoric acids), organic carboxylic acids (for example acetic, propionic, glycolic, lactic, pyruvic, malonic, succinic, fumaric, malic, tartaric, citric, ascorbic, maleic, hydroxymaleic, dihydroxymaleic, benzoic, phenylacetic, 4-aminobenzoic, 4-hydroxybenzoic, anthranilic, cinnamic, salicylic, 2-phenoxybenzoic, 2-acetoxybenzoic and mandelic acid) and organic sulfonic acids (for example methanesulfonic acid and p-toluenesulfonic acid).
- inorganic acids for example hydrochloric, hydrobromic, sulphuric and phosphoric acids
- organic carboxylic acids for example acetic, propionic, glycolic, lactic, pyruvic, malonic, succinic
- the alkaloid drugs of the invention may also be converted into salts by reaction with an alkali metal halide, for example sodium chloride, sodium iodide or lithium iodide.
- an alkali metal halide for example sodium chloride, sodium iodide or lithium iodide.
- the alkaloids of the invention are converted into their salts by reaction with a stoichiometric amount of sodium chloride in the presence of a solvent such as acetone.
- salts and the free base compounds can exist in either a hydrated or a substantially anhydrous form.
- Crystalline forms of the compounds of the invention are also contemplated and in general the acid addition salts of the alkaloids of the invention are crystalline materials which are soluble in water and various hydrophilic organic solvents and which in comparison to their free base forms, demonstrate higher melting points and an increased solubility.
- the present invention contemplates all optical isomers, racemic forms and diastereomers of the alkaloids of the invention.
- the alkaloids of the invention may exist and be synthesised and/or isolated in optically active and racemic forms.
- references to the alkaloids of the present invention encompass the alkaloids as a mixture of diastereomers, as individual diastereomers, as a mixture of enantiomers as well as in the form of individual enantiomers.
- the present invention contemplates all optical isomers and racemic forms thereof of the alkaloids of the invention, and unless indicated otherwise (e.g. by use of dash-wedge structural formulae) the compounds shown herein are intended to encompass all possible optical isomers of the compounds so depicted. In cases where the stereochemical form of the alkaloid is important for pharmaceutical utility, the invention contemplates use of an isolated eutomer.
- Alkaloids for Use Any alkaloid may be used according to the invention, providing that it induces the production of IL-2 in dendritic cells.
- Alkaloids capable of inducing the production of IL-2 in dendritic cells are herein referred to as activating alkaloids, and any suitable activating alkaloid may be used according to the invention.
- Activating alkaloids may be readily identified by screening assays (such as those described herein) designed to detect the induction of IL-2 production in dendritic cells in vitro. Such assays may involve immune assays for IL-2. Those skilled in the art will readily be able to identify appropriate conditions for such assays, including inter alia the nature and number of the dendritic cells, the relative concentrations of alkaloid and cells, the duration of stimulation with alkaloid and the methods used to detect the induction of IL-2.
- the alkaloid is isolated. However, in some embodiments the use of an isolated alkaloid is not required, and crude extracts suffice. Thus, the invention contemplates the use of herbal medicines comprising the IL-2-stimulating alkaloids of the invention.
- the alkaloids need not be naturally occurring, and may be synthetic analogues or derivatives of naturally occurring counterparts. Such analogues or derivatives are preferably pharmaceutically acceptable analogues, salts, isomers or derivatives as herein defined. However, preferred alkaloids are phytochemicals. Such phytochemicals may be isolated from natural sources or synthesised in vitro.
- alkaloids is selected from the following classes: (a) piperidines alkaloids; (b) pyrroline alkaloids; (c) pyrrolidines alkaloids; (d) pyrrolizidine alkaloids; (e) indolizidine alkaloids; (f) nortropanes alkaloids.
- alkaloid mixtures contating two or more different alkaloids representative of one or more of the classes listed above may also be used.
- the alkaloid may be polyhydroxylated.
- the polyhydroxylated alkaloid may be a sugar mimic.
- alkaloids having a small molecular weight since these may exhibit desirable pharmacokinetics.
- the alkaloid may have a molecular weight of 100 to 400 Daltons, preferably 150 to 300 Daltons and most preferably 200 to 250 Daltons.
- the alkaloids of the invention may be polar or non-polar. Preferred, however, are polar alkaloids.
- the alkaloid has the formula:
- R is selected from the group comprising hydrogen, straight or branched, unsubstituted or substituted, saturated or unsaturated acyl, alkyl (e.g. cycloalkyl), alkenyl, alkynyl and aryl groups, or a pharmaceutically acceptable salt or derivative thereof.
- alkaloids having the formula:
- R is selected from the group comprising hydrogen, straight or branched, unsubstituted or substituted, saturated or unsaturated acyl, alkyl (e.g. cycloalkyl), alkenyl, alkynyl and aryl groups, or a pharmaceutically • acceptable salt or derivative thereof.
- the alkaloid is 1R,2R,3R,6S,7S,7aR)-3-(hydroxymethyl)-1 , 2,6,7- tetrahydroxypyrrolizidine (casuarine), wherein R is hydrogen and having the formula:
- the alkaloid may also be a casuarine glycoside, or a pharmaceutically acceptable salt or derivative thereof.
- the alkaloid is preferably casuarine-6- ⁇ -D-glucoside of the formula:
- alkaloids for use according to the invention are selected from: (a) 3,7-diepi-casuarine; (b) 7-epi-casuarine; (c) 3,6,7-triepi-casuarine; (d) 6,7-diepi-casuarine; (e) 3-epi-casuarine; (f) 3,7-diepi-casuarine-6- ⁇ -D-glucoside; (g) 7-epi-casuarine-6- ⁇ -D-glucoside; (h) 3,6,7-triepi-casuarine-6- ⁇ -D-glucoside; (i) 6,7-diepi-casuarine-6- ⁇ -D-glucoside; and 0) 3-epi-casuarine-6- ⁇ -D-glucoside, or a pharmaceutically acceptable salt or derivative thereof.
- R is selected from the group comprising hydrogen, straight or branched, unsubstituted or substituted, saturated or unsaturated acyl, alkyl (e.g. cycloalkyl), alkenyl, alkynyl and aryl groups, or a pharmaceutically acceptable salt or derivative thereof.
- alkaloids having the formula: wherein R is selected from the group comprising hydrogen, straight or branched, unsubstituted or substituted, saturated or unsaturated acyl, alkyl (e.g. cycloalkyl), alkenyl, alkynyl and aryl groups, or a pharmaceutically acceptable salt or derivative thereof.
- N-hydroxyethylDMDP having the formula:
- the alkaloid has the formula:
- R 1 is selected from the group comprising hydrogen, straight or branched, unsubstituted or substituted, saturated or unsaturated acyl, alkyl (e.g. cycloalkyl), alkenyl, alkynyl and aryl groups and R 2 is selected from hydrogen, hydroxy and alkoxy, or a pharmaceutically acceptable salt or derivative thereof.
- the alkaloid preferably has the formula: wherein R 1 is selected from the group comprising hydrogen, straight or branched, unsubstituted or substituted, saturated or unsaturated acyl, alkyl (e.g. cycloalkyl), alkenyl, alkynyl and aryl groups and R 2 is selected from hydrogen, hydroxy and alkoxy, or a pharmaceutically acceptable salt or derivative thereof.
- R 1 is selected from the group comprising hydrogen, straight or branched, unsubstituted or substituted, saturated or unsaturated acyl, alkyl (e.g. cycloalkyl), alkenyl, alkynyl and aryl groups and R 2 is selected from hydrogen, hydroxy and alkoxy, or a pharmaceutically acceptable salt or derivative thereof.
- R 1 may be a saccharide moiety (for example a glucoside or arabinoside moiety).
- the alkaloid has the formula:
- the alkaloid is preferably 2-hydroxy-1 ,2-cis-castanospermine having the formula:
- the alkaloid may be 2-hydroxy-1 ,2-trans-castanospermine having the formula: or a pharmaceutically acceptable salt or derivative thereof.
- alkaloids selected from the table below:
- the alkaloids of the invention stimulate the expression IL-2 (and optionally IL-12) in dendritic cells.
- IL-2 is a Th1 cytokine involved in mediating type-1 responses. It appears to be involved not only in T cell activation but also in the activation of inter alia NK cells, so functioning to regulate and link innate and adaptive immunity. Thus, the alkaloid-induced expression of IL-2 in dendritic cells may directly potentiate a ' Th1 response and so increase the Th1 :Th2 response ratio.
- the alkaloid-induced expression of IL-2 may also indirectly potentiate a Th1 response (and so increase the Th1 :Th2 response ratio) by stimulating the activity of endogenous dendritic cells, which cells then trigger responses by other classes of lymphocytes (CTL, B, NK, and NKT cells) and also elicit T cell memory (a critical goal of vaccination).
- CTL endogenous dendritic cells
- the alkaloids of the invention may also stimulate the expression of IL-12 in lymphocytes (for example in dendritic cells and/or macrophages).
- IL-12 is the primary mediator of type-1 immunity (the Th1 response). It induces natural killer (NK) cells to produce lFN- ⁇ as part of the innate immune response and promotes the expansion of CD4 + Th1 cells and cytotoxic CD8 + cells which produce IFN- ⁇ . It therefore increases T-cell invasion of tumours as well as the susceptibility of tumour cells to T-cell invasion.
- the immunostimulatory activity of the compounds of the invention may arise from the stimulation of II-2 (and optionally IL-12) by dendritic cells.
- II-2 and optionally IL-12
- NK cells to produce lFN- ⁇ and induces the development of CD4 + Th1 cells.
- the induced Th1 cells then produce IFN- Y and IL-2.
- the IL-2 then enhances further proliferation of Th1 cells and the differentiation of pathogen (e.g. tumour and virus) -specific CD8 + T cells.
- the IL-2 also stimulates the cytolytic activity of NK cells of the innate immune system.
- an alkaloid-induced expression of IL-12 in lymphocytes by the alkaloids of the invention may directly potentiate a Th1 response and so increase the Th1 :Th2 response ratio.
- the alkaloids of the invention may also suppress the expression of one or more Th2 cytokines (e.g. IL-5), so increasing the Th1 :Th2 response ratio.
- Th2 cytokines e.g. IL-5
- the ability of the alkaloids of the invention to stimulate the expression of IL-2 in dendritic cells underpins certain important medical applications (discussed in detail infra).
- the ability of preferred alkaloids to also stimulate the expression of IL-12 (and optionally also suppress the expression of one or more Th2 cytokines) may contribute to therapeutic potency: for example, increased production of IL-12 may overcome the suppression of innate and cellular immunities of HIV-1-infected individuals and AIDS patients.
- the cytokine stimulation exhibited by the compounds of the invention may be dependent, in whole or in part, on the presence of co-stimulatory agents.
- co-stimulatory agents may include, for example, agents that stimulate the innate immune system, including Toll-like receptor (TLR) ligands.
- TLR Toll-like receptor
- ligands include microbial products such as lipopolysaccharide (LPS) and/or monophosphoryl lipid) as well as other molecules associated with microbial infection.
- LPS lipopolysaccharide
- monophosphoryl lipid monophosphoryl lipid
- tumour cell glycosylation e.g. tumour antigen glycosylation
- viral protein glycosylation e.g. virion antigen glycosylation
- Modification of cell-surface protein glycosylation in infected host cells e.g. bacterial cell walls
- the alkaloid of the invention may: (a) modify tumour cell glycosylation (e.g. tumour antigen glycosylation); and/or (b) modify viral protein glycosylation (e.g. virion antigen glycosylation); and/or (c) modify cell-surface protein glycosylation in infected host cells; and/or (d) modify bacterial cell walls, when administered in vivo.
- tumour cell glycosylation e.g. tumour antigen glycosylation
- viral protein glycosylation e.g. virion antigen glycosylation
- cell-surface protein glycosylation e.g. virion antigen glycosylation
- This optional ancillary biological activity may therefore augment the primary IL-2 inducing activity in some preferred embodiments of the invention. It may be particularly desirable in certain medical applications, including the treatment of proliferative disorders (such as cancer) or in applications where infection is attendant on immune suppression. For example, selective modification of virion antigen glycosylation may render an infecting virus less (or non-) infective and/or more susceptible to endogenous immune responses.
- the alkaloids of the invention may alter the HIV viral envelope glycoprotein gp120 glycosylation patterns, hence inhibiting the entry of HIV into the host cell by interfering with the binding to cell surface receptors.
- the alkaloids of the invention are preferably (but not necessarily) glycosidase inhibitors.
- Particularly preferred are alkaloids which exhibit specificity of glycosidase inhibition, for example Glucosidase I rather than mannosidase.
- Such preferred alkaloids can therefore be quite different in their glycosidase inhibitory profile to swainsonine and its analogues, since the latter are potent and specific inhibitors of mannosidase.
- the invention finds broad application in medicine, for example in methods of therapy, prophylaxis and/or diagnosis.
- the applications may be applied to any warm-blooded animal, including humans.
- the applications include veterinary applications, wherein the alkaloids or vaccines of the invention are administered to non- human animals, including primates, dogs, cats, horses, cattle and sheep.
- the alkaloids and vaccines of the invention have immunomodulatory activity. Thus, they find general application in the treatment or prophylaxis of conditions in which stimulation, augmentation or induction of the immune system is indicated or in which suppression or elimination of part or all of the immune response is indicated. Particular medical uses of the alkaloids of the invention are described in detail below. References to therapy and/or prophylaxis in the description or claims are to be interpreted accordingly and are intended to encompass inter alia the particular applications described below.
- the alkaloids of the invention find application in methods of therapy and/or prophylaxis which comprise increasing the Th1:Th2 response ratio (for example, by preferentially promoting a Th1 response (and optionally preferentially suppressing a Th2 response)).
- the medical applications contemplated herein therefore include any diseases, conditions or disorders in which an increase in the Th1 :Th2 response ratio is indicated or desired.
- the medical applications contemplated include diseases, conditions or disorders in which stimulation of a Th1 response and/or suppression of a Th2 response is indicated or desired.
- the ability of the alkaloids of the invention to increase the Th1 :Th2 response ratio is based, at least in part, on their ability to induce the expression of IL-2 in dendritic cells.
- the alkaloids of the invention may also induce, potentiate, activate or stimulate (either directly or indirectly) the release and/or activity (in vitro and/or in vivo) of one or more Th1 cytokines (for example one or more cytokines selected from IFN-gamma, IL-1 , TNF, IL-12, IL-2 and IL-18).
- Th1 cytokines for example one or more cytokines selected from IFN-gamma, IL-1 , TNF, IL-12, IL-2 and IL-18.
- alkaloids which also induce, potentiate, activate or stimulate the release and/or activity (in vitro and/or in vivo) of IFN-gamma and/or IL-12.
- the alkaloids of the invention may also suppress or inactivate (either directly or indirectly) the release and/or activity (in vitro and/or in vivo) of one or more Th2 cytokines (for example one or more cytokines selected from IL-4, IL-5, IL-10 and IL-13). Particularly preferred are alkaloids which suppress or inactivate the release and/or activity (in vitro and/or in vivo) of IL-5.
- alkaloids which exhibit a Th1 cytokine stimulatory activity together with a complementary Th2 cytokine inhibitory activity.
- Th1-related diseases are diseases, disorders, syndromes, conditions or infections in which Th1 cells are involved in preventing, curing or alleviating the effects of the disease, disorder, syndrome, condition or infection.
- Th1 -related diseases may also include diseases, disorders, syndromes, conditions or infections in which the Th1 component of the immune response is pathologically depressed or diseases, disorders, syndromes, conditions or infections in which stimulation of a Th1 response is indicated.
- Such conditions may arise, for example, from certain proliferative disorders (typically cancers) in which the proliferating (e.g. tumour) cells exert a suppressive effect on one or more components of the Th1 response.
- tumour cells may inhibit dendritic cells, cause the expression of inhibitory receptors on T cells, down regulate MHC class I expression and induce the secretion of anti-inflammatory factors and immunosuppressive cytokines which deactivate or suppress immune cell cytotoxicity.
- the compounds of the invention find application in the treatment or prophylaxis of Th1 -related diseases.
- Th1-related diseases include infectious diseases (particularly viral infections) and proliferative disorders (e.g. cancer).
- the Th1 -related diseases include any malignant or pre-malignant condition, proliferative or hyper- proliferative condition or any disease arising or deriving from or associated with a functional or other disturbance or abnormality in the proliferative capacity or behaviour of any cells or tissues of the body.
- the invention finds application in the treatment or prophylaxis of breast cancer, colon cancer, lung cancer and prostate cancer. It also finds application in the treatment or prophylaxis of cancers of the blood and lymphatic systems (including Hodgkin's Disease, leukemias, lymphomas, multiple myeloma, and Waldenstr ⁇ m's disease), skin cancers (including malignant melanoma), cancers of the digestive tract (including head and neck cancers, ossophageal cancer, stomach cancer, cancer of the pancreas, liver cancer, colon and rectal cancer, anal cancer), cancers of the genital and urinary systems (including kidney cancer, bladder cancer, testis cancer, prostate cancer), cancers in women (including breast cancer, ovarian cancer, gynecological cancers and choriocarcinoma) as well as in brain, bone carcinoid, nasopharyngeal, retroperitoneal, thyroid and soft tissue tumours. It also finds application in the treatment or prophylaxis
- the Th1-related infectious diseases include bacterial, prion (e.g. BSE and CJD), viral, fungal, protozoan and metazoan infections.
- the Th1 -related infectious diseases include infection with respiratory syncytial virus (RSV), hepatitis B virus (HBV), Epstein-Barr, hepatitis C virus (HCV), herpes simplex type 1 and 2, herpes genitalis, herpes keratitis, herpes encephalitis, herpes zoster, human immunodeficiency virus (HIV), influenza A virus, hantann virus (hemorrhagic fever), human papilloma virus (HPV), tuberculosis, leprosy and measles.
- RSV respiratory syncytial virus
- HBV hepatitis B virus
- HCV hepatitis C virus
- herpes simplex type 1 and 2 herpes genitalis
- Th1 -related infectious diseases include those in which the pathogen occupies an intracellular compartment, including HIV/AIDS, leishmaniasis, trypanosomiasis, influenza, tuberculosis and malaria.
- the compounds of the invention may also find application in the treatment of patients in which the Th1 immune response is defective.
- patients may include neonates, juveniles in which the Th1 response is immature and not fully developed, as well as older patients in which the Th1 response has become senescent or compromised over time.
- the compounds of the invention may be used prophylactically (as a generalized type 1 immune stimulant to reduce the risks of (e.g. viral) infections.
- Th2-related diseases are diseases, disorders, syndromes, conditions or infections in which Th2 cells are implicated in (e.g. support, cause or mediate) the effects of the disease, disorder, syndrome, condition or infection.
- the alkaloids of the invention find application in the treatment or prophylaxis of Th2-related diseases.
- Th2-related diseases treatable with the alkaloids of the invention is allergic disease.
- allergy is used to define a state of hypersensitivity induced by exposure to a particular antigen (allergen) resulting in harmful and/or uncomfortable immunologic reactions on subsequent exposures to the allergen.
- the harmful, uncomfortable and/or undesirable immunologic reactions present in allergy include a wide range of symptoms. Many different organs and tissues may be affected, including the gastrointestinal tract, the skin, the lungs, the nose and the central nervous system. The symptoms may include abdominal pain, abdominal bloating, disturbance of bowel function, vomiting, rashes, skin irritation, wheezing and shortness of breath, nasal running and nasal blockage, headache and mood changes. In severe cases the cardiovascular and respiratory systems are compromised and anaphylactic shock leads in extreme cases to death.
- the alkaloids of the invention may suppress or inactivate (either directly or indirectly) the release and/or activity (in vitro and/or in vivo) of one or more Th2 cytokines (for example one or more cytokines selected from IL-4, IL-5, IL-10 and IL-13).
- the alkaloids of the invention may be used to effect a remedial or palliative modulation of the harmful and/or uncomfortable immunologic reactions characteristic of allergic reactions by inhibiting, suppressing or eliminating the Th2 response to the allergen.
- the alkaloids of the invention therefore find application in the treatment or prophylaxis of allergy.
- Any allergy may be treated according to the invention, including atopic allergy, allergic rhinitis, allergic conjunctivitis, atopic dermatitis, hypereosinophilia, irritable bowel syndrome, allergen-induced migraine, bacterial allergy, bronchial allergy (asthma), contact allergy (dermatitis), delayed allergy, pollen allergy (hay fever), drug allergy, sting allergy, bite allergy, gastrointestinal or food allergy (including that associated with inflammatory bowel disease, including ulcerative colitis and Crohn's disease) and physical allergy.
- Physical allergies include cold allergy (cold urticaria or angioedema), heat allergy (cholinergic urticaria) and photosensitivity. Particularly important is the treatment or prophylaxis of asthma.
- the alkaloids of the invention increase splenic and bone marrow cell proliferation and can act as myeloproliferative agents. They therefore find application as haemorestoratives.
- Haemorestoration may be indicated following immunosuppressant therapies (such as cyclosporine A, azathioprine or immunosuppressant radiotherapies), chemotherapy (including treatment with both cycle-specific and non-specific chemotherapeutic agents), steroid administration or other forms of surgical or medical intervention (including radiotherapy).
- immunosuppressant therapies such as cyclosporine A, azathioprine or immunosuppressant radiotherapies
- chemotherapy including treatment with both cycle-specific and non-specific chemotherapeutic agents
- steroid administration including radiotherapy.
- the use of the alkaloids of the invention as haemorestoratives may be adjunctive to other treatments which tend to depress splenic and bone marrow cell populations.
- adjunctive therapies include the administration of an immunorestorative dose of the alkaloid of the invention adjunctive to: (a) chemotherapy; and/or (b) radiotherapy; and/or (c) bone marrow transplantation; and/or (d) haemoablative immunotherapy.
- the alkaloids of the invention may be used to alleviate, control or modify states in which the immune system is partially or completely suppressed or depressed. Such states may arise from congenital (inherited) conditions, be acquired (e.g. by infection or malignancy) or induced (e.g. deliberately as part of the management of transplants or cancers).
- the alkaloids of the invention may find application as adjunctive immunomodulators (e.g. immunostimulants) in the treatment and/or management of various diseases (including certain cancers) or medical interventions (including radiotherapy, immunosupressant therapies (such as the administration of cyclosporine A, azathioprine or immunosuppressant radiotherapies), chemotherapy and cytotoxic drug administration (for example the administration of ricin, cyclophosphamide, cortisone acetate, vinblastine, vincristine, adriamycin, 6-mercaptopurine, 5-fluorouracil, mitomycin C, chloramphenicol and other steroid-based therapies). They may therefore be used as chemoprotectants in the management of various cancers and infections (including bacterial and viral infections, e.g. HIV infection) or to induce appropriate and complementary immunotherapeutic activity during conventional immunotherapy.
- immunosupressant therapies such as the administration of cyclosporine A, azathioprine or immunosuppressant radiotherapie
- the alkaloids of the invention may find application as immunostimulants in the treatment or management of microbial infections which are associated with immune-suppressed states, including many viral infections (including HIV infection in AIDS) and in other situations where a patient has been immunocompromised (e.g. following infection with hepatitis C, or other viruses or infectious agents including bacteria, fungi, and parasites, in patients undergoing bone marrow transplants, and in patients with chemical or tumor-induced immune suppression).
- microbial infections which are associated with immune-suppressed states, including many viral infections (including HIV infection in AIDS) and in other situations where a patient has been immunocompromised (e.g. following infection with hepatitis C, or other viruses or infectious agents including bacteria, fungi, and parasites, in patients undergoing bone marrow transplants, and in patients with chemical or tumor-induced immune suppression).
- diseases or disorders which may give rise to an immunosupressed state treatable according to the invention include: ataxia-telangiectasia; DiGeorge syndrome; Chediak-Higashi syndrome; Job syndrome; leukocyte adhesion defects; panhypogammaglobulinemia (e.g. associated with Bruton disease or congenital agammaglobulinemia); selective deficiency of IgA; combined immunodeficiency disease; Wiscott-Aldrich syndrome and complement deficiencies. It may be associated with organ and/or tissue (e.g. bone marrow) transplantation or grafting, in which applications the alkaloids of the invention may be used adjunctively as part of an overall treatment regimen including surgery and post-operative management of immune status. ff) Cytokine Stimulation
- the alkaloids of the invention may be used to induce, potentiate or activate IL-2 in vivo (and optionally other cytokines, including other Th1 cytokines e.g. IL-12).
- the alkaloids of the invention find general application in the treatment or prophylaxis of conditions in which the in vivo induction, potentiation or activation of IL-2 is indicated (and optionally in the treatment or prophylaxis of conditions in which the in vivo induction, potentiation or activation of one or more other Th1 cytokines (e.g. IL-12) is also indicated.
- Th1 cytokines e.g. IL-12
- Such applications may be employed to stimulate particular elements of the cellular immunity system, including dendritic cells, macrophages (e.g. tissue-specific macrophages), CTL, NK, NKT, B and LAK cells.
- the alkaloids of the invention may be employed as an adjunct to gene therapies designed to increase the production of endogenous cytokines (for example IL-2).
- endogenous cytokines for example IL-2
- the alkaloids of the invention finds application in the treatment of proliferative disorders, including various cancers and cancer metastasis.
- the alkaloids of the invention may find particular application in the treatment of leukemias, lymphomas, melanomas (including melanoma of the eye), adenomas, sarcomas, carcinomas of solid tissues, melanoma, pancreatic cancer, cervico-uterine cancer, cancers of the kidney, stomach, lung, ovary, rectum, breast, prostate, bowel, gastric, liver, thyroid, neck, cervix, salivary gland, leg, tongue, lip, bile duct, pelvis, mediastinum, urethra, lung, bladder, esophagus and colon, and Kaposi's Sarcoma (e.g. when associated with AIDS).
- the alkaloids of the invention may exhibit a secondary glycosidase inhibitory activity.
- tumour cell glycosylation e.g. tumour antigen glycosylation
- viral protein glycosylation e.g. virion antigen glycosylation
- cell-surface protein glycosylation in infected host cells and/or the modification of bacterial cell walls, hence promoting an increased immune response or inhibiting growth/infectivity directly.
- the pyrrolizidine compounds of the invention find utility as vaccine adjuvants, in which embodiments they may promote, induce or enhance an immune response to antigens, particularly antigens having low intrinsic immunogenicity.
- the pyrrolizidine compounds of the invention may augment vaccine immunogenicity by stimulating cytokine release, thereby promoting T-cell help for B cell and CTL responses. They may also change glycosylation of cancer or viral antigens and increase vaccine effectiveness.
- the compounds of the invention may be administered concurrently, separately or sequentially with administration of the vaccine.
- the invention finds application in any vaccine, but may be particularly as a subunit vaccine, a conjugate vaccine, a DNA vaccine, a recombinant vaccine or a mucosal vaccine.
- the vaccine may be therapeutic or prophylactic. It may be used immunoprophylactically or immunotherapeutically in both human and non-human subjects. Preferred non-human subjects include mammals and birds. Particularly preferred are veterinary applications. Such applications include the treatment or prophylaxis of infection in domesticated animals (for example dogs and cats) and livestock (e.g. sheep, cows, pigs, horses, chickens and turkeys).
- the pyrrolizidine compound of the invention may be present in admixture with other vaccine components ), or else co-packaged (e.g. as part of an array of unit doses) with the other vaccine components with which it is to be used as adjuvant.
- the use of the pyrrolizidine compounds of the invention as adjuvant is simply reflected in the content of the information and/or instructions co-packaged with the vaccine components and relating to the vaccination procedure, vaccine formulation and/or posology.
- the alkaloids of the invention induce sustained and pronounced IL-2 production in dendritic cells.
- the alkaloids of the invention find application in methods of therapy or prophylaxis comprising the induction of cytokine production in dendritic cells or in which the induction of cytokine production in dendritic cells is indicated or required.
- the alkaloids of the invention may also induce the production of one or more other Th1 cytokines (for example IL-12) in dendritic cells.
- Th1 cytokines for example IL-12
- the cells are loaded (pulsed, primed or spiked) with a particular antigen or antigens and then administered to promote a Th1 immune response.
- the responding T cells include helper cells, especially Th1 CD4 + cells (which produce IFN- ⁇ ) and killer cells (especially CD8 + cytolytic T lymphocytes).
- the dendritic cells may also mediate responses by other classes of lymphocytes (B, NK, and NKT cells). They may also elicit T cell memory, a critical goal of vaccination.
- the antigens can be xenoantigens or autoantigens.
- One or more defined neoantigen(s) may be selected: in the case of cancer treatment, the neoantigen(s) may comprise a tumour-associated antigen.
- most preferred for use according to the invention are peptides (for example, synthetic 9-11 amino acid peptides) containing defined antigens. Such peptides may comprise natural sequences. Alternatively, they may be synthetic analogues designed for enhanced MHC binding.
- the antigens used according to the invention are provided in the form of immune complexes.
- dendritic cell vaccines can be used according to the invention for inducing both CTLs and Th cells.
- whole antigenic repertoire of any given tumour or other target cell, such as a virally-infected cell
- DC-tumour cell hybrids in which the dendritic cells are treated with alkaloid (thereby to induce the expression of IL-2) before or after hybridisation.
- necrotic or apoptotic tumour cells or cell lysates for example lysates of infected cells or tumour cells are used.
- Antigens derived from fresh tumour cells may also be employed.
- antigen loading various techniques can be used to deliver the selected antigen(s) to the DCs (variously referred to in the art as antigen loading, pulsing, priming or spiking).
- loading techniques which load the DCs internally: this can be achieved through the use of peptides linked to cell-penetrating moieties.
- Antigens can also be loaded by transfecting the DCs with encoding nucleic acid (e.g. by electroporation) such that the antigens are expressed by the DC, processed and presented at the cell surface.
- This approach avoids the need for expensive GMP proteins and antibodies.
- RNA is preferred for this purpose, since it produces only transient expression (albeit sufficient for antigen processing) and avoids the potential problems associated with the integration of DNA and attendant long-term expression/mutagenesis.
- Such transfection techniques also permit exploration of the whole antigenic repertoire of a target cell by use of total or PCR-amplified tumour RNA.
- the present invention also contemplates a more general approach to DC cell-based therapy which involves the stimulation of the dendritic cells with the alkaloid of the invention irrespective of the antigens present and either with or without antigen priming.
- the invention finds application in therapies in which dendritic cells exposed to the alakloid of the invention (and so induced to express IL-2) are targeted to diseased or infected tissue (for example injected directly into a tumour), where the IL-2 expressing dendritic cells can prime endogenous T cells extranodally.
- the invention contemplates targeting of DCs to a tumour and their activation in situ to elicit immune responses without the need for ex vivo antigen loading.
- the invention contemplates in situ DC vaccination where antigen is targeted to DCs in vivo which are then expanded and induced to mature in situ (by the co-administration of one or more DC maturation stimulants).
- antigen is targeted to endogenous DCs by any convenient method, for example through the use of exosomes (as described in Thery ef al. (2002) Nat Rev Immunol 2: 569-579).
- the dendritic cells may be myeloid or lymphoid, or mixtures thereof.
- the myeloid dendritic cells if used, may be of the Langerhans cell type or interstitial DCs. Alternatively, mixtures of these myeloid subsets may be used. Especially preferred is the use of monocyte-derived DCs (Mo-DCs).
- Helper proteins may be used to potentiate the activity of the dendritic cell vaccines of the invention.
- the dendritic cell based vaccines of the invention find particular application in the treatment or prophylaxis of various proliferative disorders (including various cancers, as described below).
- the dendritic cells are preferably loaded (pulsed, primed or spiked) with one or more tumour antigens ex vivo and the alkaloids of the invention used to potentiate the dendritic cell component of the vaccine by contacting the dendritic cells with the alkaloid either ex vivo (before or after pulsing of the cells) or in vivo (for example by co- administration, either concurrently, separately or sequentially, of the dendritic cells and the alkaloid).
- the dendritic cell based vaccines of the invention may be used in the treatment or prophylaxis of any malignant or pre-malignant condition, proliferative or hyper-proliferative condition or any disease arising or deriving from or associated with a functional or other disturbance or abnormality in the proliferative capacity or behaviour of any cells or tissues of the body.
- the invention finds application in the treatment or prophylaxis of breast cancer, colon cancer, lung cancer and prostate cancer. It also finds application in the treatment or prophylaxis of cancers of the blood and lymphatic systems (including Hodgkin's Disease, leukemias, lymphomas, multiple myeloma, and Waldenstrom's disease), skin cancers (including malignant melanoma), cancers of the digestive tract (including head and neck cancers, oesophageal cancer, stomach cancer, cancer of the pancreas, liver cancer, colon and rectal cancer, anal cancer), cancers of the genual and urinary systems (including kidney cancer, bladder cancer, testis cancer, prostate cancer), cancers in women (including breast cancer, ovarian cancer, gynecological cancers and choriocarcinoma) as well as in brain, bone carcinoid, nasopharyngeal, retroperitoneal, thyroid and soft tissue tumours. It also finds application in the treatment or prophylaxis of cancers of
- the dendritic cell based vaccines of the invention also find application in the treatment or prophylaxis of various infections, including bacterial, viral, fungal, protozoan and metazoan infections.
- the vaccines may be used in the treatment or prophylaxis of infection with respiratory syncytial virus (RSV), Epstein-Barr, hepatitis B virus (HBV), hepatitis C virus (HCV), herpes simplex type 1 and 2, herpes genitalis, herpes keratitis, herpes encephalitis, herpes zoster, human immunodeficiency virus (HIV), influenza A virus, hantann virus (hemorrhagic fever), human papilloma virus (HPV), tuberculosis, leprosy and measles.
- RSV respiratory syncytial virus
- HBV hepatitis B virus
- HCV hepatitis C virus
- herpes simplex type 1 and 2 herpe
- the treatment or prophylaxis of infections in which the pathogen occupies an intracellular compartment or causes the expression of neoantige ⁇ s by host cells, including HIV/AIDS, leishmania, influenza, tuberculosis and malaria.
- the pathogen occupies an intracellular compartment or causes the expression of neoantige ⁇ s by host cells, including HIV/AIDS, leishmania, influenza, tuberculosis and malaria.
- Dendritic cells are also involved in regulating and maintaining immunological tolerance: in the absence of maturation, the cells induce antigen-specific silencing or tolerance. Thus, in another dendritic cell-based treatment paradigm the cells are administered as part of an immunomodulatory intervention designed to combat autoimmune disorders.
- dendritic cells have been enhanced by in vitro transfection with genes encoding cytokines.
- gene therapy approaches are inherently dangerous and a more efficient and attractive approach would be to pulse dendritic cells in vitro with biologically active compounds which stimulate an appropriate cytokine secretion pattern in the dendritic cells.
- the alkaloids of the invention can induce sustained and pronounced IL-2 (and optionally other Th1 cytokines, e.g. IL-12) production in dendritic cells.
- the alkaloids of the invention find application in the enhancement of the suppressive potential of dendritic cells.
- the invention finds application in the treatment or prophylaxis of autoimmune disorders, including myasthenia gravis, rheumatoid arthritis, systemic lupus erythematosus, Sjogren syndrome, scleroderma, polymyositis and dermomyositis, ankylosing spondylitis, and rheumatic fever, insulin-dependent diabetes, thyroid diseases (including Grave's disease and Hashimoto thyroiditis), Addison's disease, multiple sclerosis, psoriasis, inflammatory bowel disease, and autoimmune male and female infertility.
- autoimmune disorders including myasthenia gravis, rheumatoid arthritis, systemic lupus erythematosus, Sjogren syndrome, scleroderma, polymyositis and dermomyositis, ankylosing spondylitis, and rheumatic fever, insulin-dependent diabetes, thyroid diseases (including Grave's disease
- Some alkaloids of the invention can reverse a Th2 type splenocyte response ex vivo in a normally non-healing infectious disease model.
- Antigen specific splenocyte IFN-gamma can be significantly increased and IL-5 production significantly reduced in such models, indicative of a healing response.
- the invention finds application in the treatment of wounds.
- the invention finds application in the treatment or prophylaxis of wounds and lesions, for example those associated with post-operative healing, burns, infection (e.g. necrotic lesions), malignancy or trauma (e.g. associated with cardiovascular disorders such as stroke or induced as part of a surgical intervention).
- the wound treatments may involve the selective suppression or elimination of a Th2 response (for example to eliminate or suppress an inappropriate or harmful inflammatory response).
- the alkaloids of the present invention can be administered by oral or parenteral routes, including intravenous, intramuscular, intraperitoneal, subcutaneous, transdermal, airway (aerosol), rectal, vaginal and topical (including buccal and sublingual) administration.
- oral or parenteral routes including intravenous, intramuscular, intraperitoneal, subcutaneous, transdermal, airway (aerosol), rectal, vaginal and topical (including buccal and sublingual) administration.
- Preferred routes of administration of the dendritic cell vaccines of the invention are subcutaneous or transdermal routes.
- the amount of the alkaloid administered can vary widely according to the particular dosage unit employed, the period of treatment, the age and sex of the patient treated, the nature and extent of the disorder treated, and the particular alkaloid selected.
- alkaloids of the invention can be used in conjunction with other agents known to be useful in the treatment of diseases, disorders or infections where immunostimulation is indicated (as described infra) and in such embodiments the dose may be adjusted accordingly.
- the effective amount of the alkaloid administered will generally range from about 0.01 mg/kg to 500 mg/kg daily.
- a unit dosage may contain from 0.05 to 500 mg of the alkaloid, and can be taken one or more times per day.
- the alkaloid can be administered with a pharmaceutical carrier using conventional dosage unit forms either orally, parenterally, or topically, as described below.
- a suitable dose will be in the range of 0.01 to 500 mg per kilogram body weight of the recipient per day, preferably in the range of 0.1 to 50 mg per kilogram body weight per day and most preferably in the range 1 to 5 mg per kilogram body weight per day.
- the desired dose is preferably presented as a single dose for daily administration. However, two, three, four, five or six or more sub-doses administered at appropriate intervals throughout the day may also be employed. These sub-doses may be administered in unit dosage forms, for example, containing 0.001 to 100 mg, preferably 0.01 to 10 mg, and most preferably 0.5 to 1.0 mg of active ingredient per unit dosage form.
- compositions of the invention comprise the alkaloid of the invention, optionally together with a pharmaceutically acceptable excipient.
- the alkaloid of the invention may take any form. It may be synthetic, purified or isolated from natural sources (for example from Casuarina equisetifolia or Eugenia jambolana), using techniques described in the art (and referenced infra). When isolated from a natural source, the alkaloid of the invention may be purified.
- the compositions of the invention may take the form of herbal medicines, as hereinbefore defined. Such herbal medicines preferably are analysed to determine whether they meet a standard specification prior to use.
- the herbal medicines for use according to the invention may be dried plant material.
- the herbal medicine may be processed plant material, the processing involving physical or chemical pre-processing, for example powdering, grinding, freezing, evaporation, filtration, pressing, spray drying, extrusion, supercritical solvent extraction and tincture production.
- the plant material may be dried prior to use. Any convenient form of drying may be used, including freeze-drying, spray drying or air-drying.
- any suitable excipient may be used, including for example inert diluents, disintegrating agents, binding agents, lubricating agents, sweetening agents, flavouring agents, colouring agents and preservatives.
- suitable inert diluents include sodium and calcium carbonate, sodium and calcium phosphate, and lactose, while com starch and alginic acid are suitable disintegrating agents.
- Binding agents may include starch and gelatin, while the lubricating agent, if present, will generally be magnesium stearate, stearic acid or talc.
- compositions may take any suitable form, and include for example tablets, elixirs, capsules, solutions, suspensions, powders, granules and aerosols.
- the pharmaceutical composition may take the form of a kit of parts, which kit may comprise the composition of the invention together with instructions for use and/or a plurality of different components in unit dosage form.
- Tablets for oral use may include the alkaloid of the invention, either alone or together with other plant material associated with the botanical source(s) (in the case of herbal medicine embodiments).
- the tablets may contain the alkaloid of the invention mixed with pharmaceutically acceptable excipients, such as inert diluents, disintegrating agents, binding agents, lubricating agents, sweetening agents, flavouring agents, colouring agents and preservatives.
- suitable inert diluents include sodium and calcium carbonate, sodium and calcium phosphate, and lactose, while corn starch and alginic acid are suitable disintegrating agents.
- Binding agents may include starch and gelatin, while the lubricating agent, if present, will generally be magnesium stearate, stearic acid or talc. If desired, the tablets may be coated with a material such as glyceryl monostearate or glyceryl distearate, to delay absorption in the gastrointestinal tract.
- Capsules for oral use include hard gelatin capsules in which the alkaloid of the invention is mixed with a solid diluent, and soft gelatin capsules wherein the active ingredient is mixed with water or an oil such as peanut oil, liquid paraffin or olive oil.
- Formulations for rectal administration may be presented as a suppository with a suitable base comprising for example cocoa butter or a salicylate.
- Formulations suitable for vaginal administration may be presented as pessaries, tampons, creams, gels, pastes, foams or spray formulations containing in addition to the active ingredient such carriers as are known in the art to be appropriate.
- the compounds of the invention will generally be provided in sterile aqueous solutions or suspensions, buffered to an appropriate pH and isotonicity.
- Suitable aqueous vehicles include Ringer's solution and isotonic sodium chloride.
- Aqueous suspensions according to the invention may include suspending agents such as cellulose derivatives, sodium alginate, polyvinylpyrrolidone and gum tragacanth, and a wetting agent such as lecithin.
- Suitable preservatives for aqueous suspensions include ethyl and n-propyl p-hydroxybenzoate.
- the compounds of the invention may also be presented as liposome formulations.
- the alkaloid of the invention can be formulated into solid or liquid preparations such as capsules, pills, tablets, troches, lozenges, melts, powders, granules, solutions, suspensions, dispersions or emulsions (which solutions, suspensions dispersions or emulsions may be aqueous or non-aqueous).
- the solid unit dosage forms can be a capsule which can be of the ordinary hard- or soft-shelled gelatin type containing, for example, surfactants, lubricants, and inert fillers such as lactose, sucrose, calcium phosphate, and cornstarch.
- the alkaloids of the invention are tableted with conventional tablet bases such as lactose, sucrose, and cornstarch in combination with binders such as acacia, cornstarch, or gelatin, disintegrating agents intended to assist the break-up and dissolution of the tablet following administration such as potato starch, alginic acid, corn starch, and guar gum, lubricants intended to improve the flow of tablet granulations and to prevent the adhesion of tablet material to the surfaces of the tablet dies and punches, for example, talc, stearic acid, or magnesium, calcium, or zinc stearate, dyes, coloring agents, and flavoring agents intended to enhance the aesthetic qualities of the tablets and make them more acceptable to the patient.
- conventional tablet bases such as lactose, sucrose, and cornstarch in combination with binders such as acacia, cornstarch, or gelatin
- disintegrating agents intended to assist the break-up and dissolution of the tablet following administration such as potato starch, alginic acid, corn starch
- Suitable excipients for use in oral liquid dosage forms include diluents such as water and alcohols, for example, ethanol, benzyl alcohol, and the polyethylene alcohols, either with or without the addition of a pharmaceutically acceptably surfactant, suspending agent or emulsifying agent.
- the alkaloids of the invention may also be administered parenterally, that is, subcutaneously, intravenously, intramuscularly, or interperitoneally.
- the alkaloid is provided as injectable doses in a physiologically acceptable diluent together with a pharmaceutical carrier (which can be a sterile liquid or mixture of liquids).
- a pharmaceutical carrier which can be a sterile liquid or mixture of liquids.
- suitable liquids include water, saline, aqueous dextrose and related sugar solutions, an alcohol (such as ethanol, isopropanol, or hexadecyl alcohol), glycols (such as propylene glycol or polyethylene glycol), glycerol ketals (such as 2,2- dimethyl-1 ,3-dioxolane-4-methanol), ethers (such as poly(ethylene-glycol) 400), an oil, a fatty acid, a fatty acid ester or glyceride, or an acetylated fatty acid glyceride with or without the addition of a pharmaceutically acceptable surfactant (such as a soap or a detergent), suspending agent (such as pect
- Suitable oils which can be used in the parenteral formulations of this invention are those of petroleum, animal, vegetable, or synthetic origin, for example, peanut oil, soybean oil, sesame oil, cottonseed oil, corn oil, olive oil, petrolatum, and mineral oil.
- Suitable fatty acids include oleic acid, stearic acid, and isostearic acid.
- Suitable fatty acid esters are, for example, ethyl oleate and isopropyl myristate.
- Suitable soaps include fatty alkali metal, ammonium, and triethanolamine salts and suitable detergents include cationic detergents, for example, dimethyl dialkyl ammonium halides, alkyl pyridinium halides, and alkylamines acetates; anionic detergents, for example, alkyl, aryl, and olefin sulphonates, alkyl, olefin, ether, and monoglyceride sulphates, and sulphosuccinates; nonionic detergents, for example, fatty amine oxides, fatty acid alkanolamides, and polyoxyethylenepolypropylene copolymers; and amphoteric detergents, for example, alkyl-beta-aminopropionates, and 2-alkylimidazoIine quarternary ammonium salts, as well as mixtures.
- suitable detergents include cationic detergents, for example, dimethyl dialkyl ammonium halides, alky
- compositions of this invention will typically contain from about 0.5 to about 25% by weight of the alkaloid of the invention in solution. Preservatives and buffers may also be used. In order to minimize or eliminate irritation at the site of injection, such compositions may contain a non-ionic surfactant having a hydrophile-lipophile balance (HLB) of from about 12 to about 17. The quantity of surfactant in such formulations ranges from about 5 to about 15% by weight.
- the surfactant can be a single component having the above HLB or can be a mixture of two or more components having the desired HLB.
- surfactants used in parenteral formulations are the class of polyethylene sorbitan fatty acid esters, for example, sorbitan monooleate and the high molecular weight adducts of ethylene oxide with a hydrophobic base, formed by the condensation of propylene oxide with propylene glycol.
- the alkaloids of the invention may also be administered topically, and when done so the carrier may suitably comprise a solution, ointment or gel base.
- the base for example, may comprise one or more of the following: petrolatum, lanolin, polyethylene glycols, bee wax, mineral oil, diluents such as water and alcohol, and emulsifiers and stabilizers.
- Topical formulations may contain a concentration of the alkaloid from about 0.1 to about 10% w/v (weight per unit volume).
- the alkaloids of the invention may be formulated for use with one or more other drug(s).
- the alkaloids of the invention may be used in combination with antitumor agents, antimicrobial agents, anti-inflammatories, antiproliferative agents and/or other immunostimulatory agents.
- the alkaloids of the invention may be used with anti-viral and/or anti-proliferative agents such as cytokines, including interleukins-2 and 12, interferons and inducers thereof, tumor necrosis factor (TNF) and/or transforming growth factor (TGF), as well as with myelosuppressive agents and/or chemotherapeutic agents (such as doxorubicin, 5-fluorouracil, cyclophosphamide and methotrexate), isoniazid (e.g. in the prevention or treatment of peripheral neuropathy) and with analgesics (e.g. NSAIDs) for the prevention and treatment of gastroduodenal ulcers.
- cytokines including interleukins-2 and 12, interferons and inducers thereof, tumor necrosis factor (TNF) and/or transforming growth factor (TGF)
- myelosuppressive agents and/or chemotherapeutic agents such as doxorubicin, 5-fluorouracil, cyclophosp
- adjunctive use may be reflected in a specific unit dosage designed to be compatible (or to synergize) with the other drug(s), or in formulations in which the alkaloid is admixed with one or more antitumor agents, antimicrobial agents and/or antiinflammatories (or else physically associated with the other drug(s) within a single unit dose).
- Adjunctive uses may also be reflected in the composition of the pharmaceutical kits of the invention, in which the alkaloid of the invention is co-packaged (e.g. as part of an array of unit doses) with the antitumor agents, antimicrobial agents and/or antiinflammatories.
- Adjunctive use may also be reflected in information and/or instructions relating to the co-administration of the alkaloid with antitumor agents, antimicrobial agents and/or antiinflammatories.
- Example 1 Induction of IL-12 secretion in dendritic cells
- mice BALB/c male and female mice bred and maintained at the University of Strathclyde under conventional conditions were used at 8 weeks old.
- Bone marrow was obtained from the femurs of mice. The femurs were washed in 70% ethanol and placed in a clean petri dish. Dendritic cell (DC) medium (2.5% granulocyte-macrophage colony-stimulating factor (GM- CSF), 10%) heat and activated foetal calf serum, 1% L-glutamine, 1% Penicillin/Streptomycin in RPMI-1640 medium) was injected into the bone marrow of the femur by a pumping action and the cells and medium were collected. 1 ml of the cells in medium was added to a 75cm 2 flask with 15m!s of DC medium. The flasks were then incubated at 37°C, 5% COa to allow DC growth and development. After 5 days an additional 10mls of DC medium was added.
- DC Dendritic cell
- the dendritic cells were harvested. This process was carried out in a tissue culture hood. The contents of the flasks were poured into centrifuge tubes to ensure collection of floating DCs. Approximately 10mls of cooled phosphate buffered saline (PBS) was added to each empty flask, the flasks gently agitated and the contents collected. This ensured recovery of adhesive DCs. The collected contents of the flasks were centrifuged for 5 minutes at 200g and the pellet resuspended in 2mls of DC medium without GM-CSF. A cell count was then carried out. Cell count and assay conditions
- Cells were counted using a haemocytometer. Approximately 20 ⁇ l of the resuspended cells was pipetted into 4 the chamber of the haemocytometer, the cells were adjusted to the correct cell- concentration (approx. 5 x 10 , and not less than 1 x 10 4 , per well) and then plated out for assay.
- the plates were incubated overnight at 37°C with 5% CO2 and allowed to settle (harvesting stimulates them). The next day the compounds (50 ⁇ g/ml and 20 ⁇ g/ml) and controls were added then again incubated at 37°C with 5% CO 2 for 24 hrs (or 48 hrs). Harvesting and addition of the compounds was all done in a hood. The plates were then frozen to kill the cells and once defrosted the supernatant analysed as described below.
- IL-12 concentration in the supernatants was measured. All reagents used in this assay were from PharMingen. A 96-well flat-bottomed ELISA plate was coated with purified rat anti-mouse IL-12 (p40/p70) MAb (Cat no. 554478) at 2 ⁇ g/ml diluted in PBS pH 9.0 at 50 ⁇ l/well. The plate was then covered in cling film and incubated at 4°C. Following incubation the plate was washed 3 times in washing buffer and dried.
- ELISA enzyme linked immunosorbent assay
- Biotin labelled anti-mouse IL-12 (p40/p70) MAb (Cat no. 18482D) at 1 ⁇ g/ml (diluted in blocking buffer) was added to each well at a volume of 100 ⁇ l/well. The plate was covered in cling film and incubated at 37°C for 1 hour. The plate was then washed 5 times, dried and the conjugate added. Streptavidin-AKP (Cat no. 13043E) at 100 ⁇ l/well was added at a dilution of 1/2000 in blocking buffer followed by incubation under cling film at 37°C for 45 minutes.
- Example 2 Stimulation of IL-2 production by dendritic cells
- Example 2 The protocols described in Example 1 above were carried out but the appropriate Mabs and standards for determination of II-2 were substituted. The results are shown in Table 2.1 , below.
- Example 3 Cytokine modulation in spleen cells Mice BALB/c male and female mice bred and maintained at the University of Strathclyde under conventional conditions were used at varying age.
- the mouse spleen was removed aseptically and placed in a sterile petri dish containing 5m!s of complete medium (RPMI, 1% L-Glutamine, 1 % Penicillin/Streptomycin and 10% foetal calf serum).
- Cells suspensions were prepared by using the end of a syringe and grinding the spleen through a wire mesh. The cell suspension was then centrifuged at lOOOrpm for 5 minutes. To remove the erythrocytes, the cell pellet was resuspended in Boyle's solution (Tri ⁇ 0.17M & Ammonium Chloride 0.16M) and centrifuged again for 5 minutes. The pellet was then washed in medium a further two times, then resuspended in 3mls medium. A cell count was then carried out.
- complete medium RPMI, 1% L-Glutamine, 1 % Penicillin/Streptomycin and 10% foetal calf serum.
- All spleen cell experiments were carried out in 96-well tissue culture plates. 100 ⁇ l aliquots of 5x10 5 /well cells were added to all wells and each well had a final volume of 200 ⁇ l. Unstimulated wells contained 10O ⁇ l of cells and 10O ⁇ l of medium. The stimulated wells contained 10O ⁇ l of cells plus 50 ⁇ l of LPS at 1 ⁇ g/ml or 50 ⁇ l anti- CD3 at 0.5 ⁇ g/ml and 50 ⁇ l of medium. The remaining wells contained 100 ⁇ l cells, 50 ⁇ l of MNLP compound and either 50 ⁇ l of anti-CD3 or medium alone.
- the reactions were stopped with 0.4M glycine (pH 10.4) during the exponential phase of the reaction, which was determined at the beginning of the assay using blanks with water, which were incubated for a range of time periods to measure the reaction rate using 5 mM substrate solution. Endpoint absorbances were read at 405nm with a Biorad microtitre plate reader (Benchmark). Water was substituted for the inhibitors in the blanks.
- mice were 3-4 weeks old female BALB/c. Mice were inoculated with 10 4 p.f.u. HSV-1 (SC16) using the neck skin method. This dose is sublethal but produces clinical symptoms, including inflammation (measured by increase in ear pinna thickness). Mice were administered (100 ml i.p.) with one of two doses of casuarine (8) on day one and daily thereafter for 5 days. Group 1 received 15 mg/kg in PBS, group 2 received 150 mg/kg in PBS. A negative control group 3 were infected but received no casuarine. A positive control group 4 were administered with famciclovir (via drinking water spiked at 1 mg/ml for the same time period). Mice were checked daily and samples were obtained from mice killed on selected days. The results are presented in Tables 6.1 - 6.3, below.
- mice (C57/bl6 under i/p ketamine anaesthesia) were challenged i/v (tail vein) with 5x10 4 B16-F10 tumour cells in a final volume of 100 ⁇ l per mouse on day 0.
- Test compounds 50mg/kg in 200 ⁇ f sterile non-pyrogenic saline
- mice were sacrifices and the lungs dissected and stained in Indian ink solution (150ml bidistilled water, 30 ml India Ink, 4 drops NH 4 OH) for 10 minutes then fixed for at least 24 hr in Fakete's solution (90ml 37% formaldehyde, 900 ml 70% EtOH and 45ml glacial acetic acid).
- Indian ink solution 150ml bidistilled water, 30 ml India Ink, 4 drops NH 4 OH
- Fakete's solution 90ml 37% formaldehyde, 900 ml 70% EtOH and 45ml glacial acetic acid.
- the metastases in the stained and fixed lungs could
- MCF-7 cells European Collection of Cell Cultures Ref. 86012803 were taken from liquid nitrogen stock, thawed at room temperature and transferred to 10ml Dulbeccos Modified Eagle's Medium with Hams F12, 15mM Hepes and L-glutamine (DMEM: Cambrex Cat. No. BE12-719F) supplemented with 10% v/v foetal calf serum (FCS: BioWest Labs Cat. No. S02755, Lot. No. S1800). The FCS was pre-filtered through a 0.2 ⁇ m steril filter.
- the cells were then centrifuged at 1 ,500 rpm in a Centaur bench-top centrifuge and the supernatant removed.
- the cells were reconstituted in fresh media and seeded into two T75cm 3 Nunclon tissue culture flasks and allowed to settle overnight at 37°C in a 5% CO2 incubator.
- the flasks were wrapped in cling film to prevent cross-contamination and the following day the media was changed to include the antibiotics penicillin and streptomycin as a precautionary measure against infection (at concentrations of mg/cm 3 and 5mg/cm 3 , respectively).
- the cells were allowed to grow near confluence and then split at a 1 in 4 resuspension.
- the cells used for the experiments were of passage number 31.
- Two flasks of cells were prepared in media containing 20% v/v FCS with 10% dimethylsulphoxide and banked down into liquid nitrogen for later use if necessary.
- the cells were harvested using a non-enzymatic method. At each of the time points the cells were viewed under the inverted light microscope and the morphology evaluated. Before harvesting, the cells were washed with sterile PBS, theree times, 7cm 3 per wash. The cells were then scraped from the flasks using a sterile cell scraper and transferred to Grenier tubes. The cells were quickly passed through a 21 G2 gauge needle to disaggregate the cells. Cells were then pelleted by centrifugation at 1500g/5min and resuspended in a known volume of PBS. The number of cells was then counted in a haemocytometer and cell viability evaluated by mixing 0.1 cm 3 of each cell suspension with a drop of trypan blue solution. Each of the cell pellets was frozen at -80°C until glycan release and analysis.
- the cell pellets were placed in an iced water bath and allowed to thaw.
- the pellets were then homogenized in a total of 4cm 3 (made up to volume with deionized water).
- An Ultraturrax T25 homogeniser was used for this purpose, with the blade speed set to 22,500 rpm.
- the samples were maintained on ice and 3 bursts, each of 10sec, were applied with a period of approximately 1 min between each homogenisation step to allow the froth the settle.
- the blade was washed carefully between each of the samples to prevent sample cross- contamination.
- the homogenates were stored in 1cm 3 aliquots at -80°C prior to the protein assay and glycan release.
- the samples were loaded onto prewashed and primed Ludger Clean E cartridges (Cat. No. LC-E10-A6).
- the glycans were eiuted according to the manufacturer's instructions and dried by centrifugal evaporation overnight.
- the glycans were labelled by reductive amination, for 2 hours at 65°C, according to the method described by Bigge ef al. (1995) Anal. Biochem. 230(2): 229-238.
- the incubation mixture was then "cleaned up” to remove any unconjugated fluorophore by spotting the samples onto Whatman 3MM paper and running in a descending chromatography tank with a mobile phase of 4:1:1 butanol:ethanol:water overnight.
- Glycans were then eiuted with 0.5cm 3 methanol and 2 x 1 cm 3 HPLC grade water then filtered through a 0.2 ⁇ m syringe top filter. Analysis using normal phase HPLC
- the glycans were separated on a normal phase (hydrophilic interaction) HPLC column (LudgerSep N1 amide) 4.6 x 25 cm in size.
- Example 10 Increasing the Th1:Th2 response ratio in a non-healing leishmaniasis model
- Leishmaniasis is a classic model of a Th1 disease: non-healing cutaneous lesions arise from an undesirable polarization of the immune response which becomes heavily Th2-skewed.
- Th1 :Th2 response ratio in this disease model (and so promote a healing Th1 response)
- spleen cells from Leishmania major infected BALB/c mice having a non-healing cutaneous infection were stimulated with parasite antigen (Table 10.1 ) or polyclonally with anti-CD3 (Table 10.2) in the presence of 3,7-diep/-casuarine (10).
- Amberiite IR- 120, strongly acidic ion-exchange resin was prepared by soaking the resin in 2M hydrochloric acid for at least two hours followed by elution with distilled water until the eluant reached pH 5.
- Dowex 50WX8-100 was prepared by soaking the resin with 2M hydrochloric acid for at least two hours followed by elution with distilled water until neutral.
- Infrared spectra were recorded on a Perkin-Elmer 1750 IR Fourier Transform spectrophotometer using thin films on sodium chloride plates. Only characteristic peaks are recorded. Optical rotations were measured on a Perkin-Elmer 241 polarimeter with a path length of 1dm. Concentrations are quoted in g/100mL.
- Nuclear magnetic resonance spectra were recorded on a Bruker DQX 400 spectrometer in the stated deuterated solvent. All spectra were recorded at ambient temperature. Chemical shifts ( ⁇ ) are quoted in ppm and are relative to residual solvent as standard. Proton spectra (5H) were recorded at 400 MHz and carbon spectra ( ⁇ c) at 100 Hz.
- the resulting foam was treated with acetone (500 ml) and sulphuric acid (5.4 ml) in the presence of anhydrous copper sulphate ( 0 g, 62 mmol) at room temperature for 48 h.
- T.l.c analysis indicated the presence of two major products (ethyl acetate:cyclohexane, 1:1 ; R f 0.72, 0.18).
- the reaction mixture was filtered and the filtrate was treated with sodium bicarbonate (50 g) for 24 h at room temperature. Solid residues were removed by filtration and the filtrate was concentrated under reduced pressure.
- Example 12 Adjuvant activity of 3.7-diep/-casuarine (MNLP 24) with an optimum dose of influenza vaccine
- mice Three groups of 6 female Balb/c mice were vaccinated intramuscularly (i.m.) with a mixture of the influenza vaccine together with 3,7-diep/-casuarine on days 0 and 14. Each group received a different dose of 3,7-diep/- casuarine. Group B received a low dose, group C a medium dose and group D a high dose (20, 50 and 100 ⁇ g respectively). A fourth control group (group A) received only the influenza vaccine. Immunomodulatory activity was assessed by the determination of influenza-specific antibody responses in serum obtained at day 28.
- mice 34 female, SPF-bred, BALB/c mice were obtained from a colony maintained under SPF-conditions at Charles River Deutschland, Sulzfeld, Germany. Twenty-four animals were allocated to the various groups by computer randomization. Mice were housed in type 2 macrolon cages in the same room throughout the study period at a temperature of 20.7-24.6°C and 30-70% humidity. The animal room was ventilated with at least 10 air changes per hour. The lighting was artificial by fluorescent tubes, time switch controlled at a sequence of 12 hours light, 12 hours dark (lights on from 7.00 a.m. to 7.00 p.m.). Feed and drinking water were provided ad libitum.
- Water for injection was supplied by NPBI (the Netherlands), batch number 03G0472, expiry date June 2006, CBS number 42650, stored at ambient room temperature.
- Influenza vaccine (Solvay Pharmaceuticals B.V, Weesp, the Netherlands) is a monovalent subunit vaccine containing haemaglutinin (HA) of the A/Panama strain. Concentration of stock was 430 ⁇ g HA/ml, batch number HPR34, stored at 2-10 ° C.
- Control group A received only influenza vaccine (105 ⁇ l influenza vaccine stock mixed with 245 ⁇ l water for injection).
- Group B received 49 ⁇ l from the stock 3,7-diep/ ' -casuarine mixed with 105 ⁇ l influenza vaccine stock and 196 ⁇ l water for injection.
- Group C received 122.5 ⁇ l from the stock 3,7-diep/-casuarine mixed with 105 ⁇ l influenza vaccine stock and 122.5 ⁇ l water for injection.
- Group D received 245 ⁇ l from the stock 3,7-diep/- casuarine mixed with 105 ⁇ l influenza vaccine stock.
- Control group A received two i.m. injections in the thigh muscle of the left and right hind leg (25 ⁇ l per paw) with vaccine at days 0 and 14.
- Groups B-D were injected i.m. in the thigh muscle of the left and right hind leg (25 ⁇ l per paw) with vaccine and 3,7-diep/-casuarine. Determination of influenza vaccine-specific IgG. lgG1 and lgG2a
- IgG, lgG1 (Th2-mediated) and lgG2a (Th1 -mediated) antibodies against vaccine antigen were determined in the serum samples obtained at days 0 and 28 using sandwich ELISA.
- Flat bottom plates (NUNC Immuno Plate, Roskilde, Denmark) were coated with 100 ⁇ l (5 ⁇ g protein/ml), dissolved in carbonate buffer (pH 9.6). After washing three times (0.5% Tween-20 solution), the plates were blocked by adding 100 ⁇ l/well PBS containing 1% bovine serum albumin and 0.02% Tween 20 (PBS/Tween 0.02%/BSA 1 %).
- test-serum in PBS/Tween 0.02%/BSA1%
- PBS/Tween 0.02%/BSA1% serial dilutions of test-serum (in PBS/Tween 0.02%/BSA1%) were incubated in duplicate (1 h, 37°C). Starting dilutions were 1 :400.
- HRP-conjugated antibody was added (30 min, 37°C); rat anti-mouse IgG, lgG1 or lgG2a (Zymed) diluted in PBS/BSA/Tween (1:1000).
- TMB-substrate solution (3,3',5,5'-tetramethylbenzidine) was added (20 min, RT), subsequently the reaction was stopped with 2N H 2 S0 .
- Optical density (OD) were measured at 450 nm using a Bio Rad microplate reader 3550 (Bio Rad Laboratories, Richmond, CA). Based upon a standard curve obtained with a reference (pooled) serum sample, containing an arbitrary number of units of specific antibodies per ml (AU/ml), concentrations of influenza-specific IgG, lgG1 and lgG2a in serum was calculated.
- Reference serum was prepared by mixing equal volumes of each individual serum sample obtained at day 28 from group A and was incorporated on each individual ELISA plate (starting dilution 1 :50, serially diluted 11 times).
- Example 13 Anti-metastatic activity of casuarine The ability of casuarine to prevent the formation of lung colonies in C57BL/6 mice following the intravenous administration of metastatic murine B16/F10 melanoma cells was demonstrated.
- the dose volume was the daily intake for all animals. Test substances were administered to mice in Groups 2, 3, 4 and 5, as solutions in sterile water for 41 h 32 min prior to tumour cell injection (Day 0 is the day of tumour cell injection).
- mice were injected intravenously, via a tail vein, with 0.1 ml of a suspension of B16/F10 melanoma cells (approximately 1 x 10 5 cells/mouse) on Day 0.
- Casuarine was formulated for dosing by preparing a 3 ⁇ g/ml solution in sterile water. All dosing solutions were prepared on the first day of dosing (Day -2) and were kept at room temperature.
- mice Female C57BL/6 mice were supplied and delivered by Charles River UK Limited. The animals were approximately 6-8 weeks of age on arrival. The animals were acclimatized for 29 days prior to the start of the study, and the body weights at the start of dosing were in the range 18-23 g. During the study, the animals were housed in groups of up to 6 in paediatric filter-topped cages with sawdust bedding (autoclaved for sterility). The room and cages were cleaned at regular intervals each week to maintain hygiene. The mice were fed an expanded rodent diet of RM1(E) SQC (batch number 3822; Special Diets Services, Witham, UK) ad libitum and allowed access to sterilized water (N.V.
- RM1(E) SQC batch number 3822; Special Diets Services, Witham, UK
- the drinking water also contained the appropriate level of test substance for the duration of the dosing period.
- the holding rooms had a 12 h light-dark cycle (on 07:00, off 19:00), and were air-conditioned by a system designed to maintain air temperature within the range 20 ⁇ 3°C. During the acclimatization and study period the temperature range in the rooms was 18-22°C and the humidity range was 44-79%.
- Tumour challenge B16/F10 murine tumour cells (Shrayer D, Bogaars H, Hearing V.J. Maizel A. & Wanebo H. Further characterization of a clinically relevant model of melanoma metastasis and an effective vaccine. Cancer Immunology, Immunotherapy 1995: 40; 277-282) were harvested from sub-confluent cultures growing in vitro, using minimum trypsination. They were washed in sterile phosphate buffered saline (PBS) and the number of viable cells determined. Cells were resuspended in sterile PBS at a concentration of 1 x 10 6 cells/ml.
- PBS sterile phosphate buffered saline
- mice were intravenously injected, via a tail vein, with 0.1 ml of cell suspension (i.e. approximately 1 x 10 5 cells/mouse) on Day 0.
- the mice were examined daily for signs of laboured breathing or distress due to extensive metastatic disease.
- Mice were exposed to the appropriate test substance for 41 h 32 min prior to tumour cell injection (from Day -2 to Day 0). Following tumour cell implant, the test substances were removed, and animals in all groups received drinking water alone for the remainder of the study. Sampling and analysis: The animals were terminated on Day 21 (21 days after injection of the tumour cells). Blood samples were taken from 3 mice from each group on the day of termination. Animals were anaesthetized with carbon dioxide and blood samples of maximal volume taken by cardiac puncture and collected in plain tubes.
- mice were left to clot at room temperature for a minimum of 1 h prior to serum removal. Serum samples were stored at approximately -70°C prior to analysis. Immediately after performing the bleeds, the spleens were removed from the 3 selected mice. The spleens from these mice were pooled in a petri dish with approximately 3-5 ml of RPMI 1640 tissue culture medium (batch number 3092202; Invitrogen, Paisley, UK) containing 0.52% penicillin/streptomycin (Pen/Strep; batch number 44K2412; Sigma Aldrich, Poole, UK) prior to splenocyte isolation.
- the spleens from each group were placed in a glass homogeniser with an additional 10-15 ml of medium and homogenised until opaque. The suspension was then placed in a 50 ml centrifuge tube. On occasions when there was significant cell debris, this was allowed to settle before removing the suspension and placing in a fresh tube. The homogenate was then centrifuged at approximately 200 x 'g' for 5 min at approximately 4°C. After discarding the supernatant, the cell suspension was resuspended in 3 ml RPM1 1640 tissue culture medium containing Pen/Strep as detailed previously.
- Ammonium chloride lysing solution (42 mi of 1x solution; batch number 0000081656; BD Biosciences, Oxford, UK) was added to each tube, and mixed by inversion. After standing for 3-5 min at room temperature, the cell suspension was centrifuged at approximately 200 x 'g' for 5 min at room temperature. The supernatant was again discarded, and the cell pellet resuspended in 30 ml RPMI 1640 tissue culture medium containing Pen/Strep as described above. Equal volumes of cell suspension and Trypan blue (50 ⁇ L of each; batch number 034K2375; Sigma Aldrich, Poole, UK) were mixed, and the number of viable cells were counted with a haemocytometer using the Trypan blue method.
- the cell suspension was then centrifuged at approximately 200 x 'g' for 5 min at room temperature, the supernatant discarded, and the cell pellet resuspended in freezing medium (batch number 054K0495; Sigma Aldrich, Poole, UK) at 1.5 x 10 7 cells/ml (Groups 1, 2 and 5) or 1.0 x 10 7 cells/ml (Groups 3 and 4).
- the cells were then split into 1 ml aliquots in sterile cryovials, and stored in liquid nitrogen prior to dispatch to the Sponsor for analysis.
- the lungs from each animal were removed and weighed prior to inflation with formalin (batch number 1273764/3; VWR International Ltd., Poole, UK). After fixation, the number of colonies on the surface of each set of lungs was counted by eye. Analysis: The number of lung colonies was counted and the data used for statistical analysis. Due to variability in the results (arising in part from coalescing tumours on the lungs of some negative control animals leading to gross underestimation of colony numbers), outlying values in the data were determined by performing the Grubb's test on the colony counts. Three outliers were identified from this analysis and excluded. The corrected data were then analysed by one-way analysis of variance (ANOVA) followed by a Dunnett's multiple comparison test to determine the significance of the effect of the test substances on the number of colonies formed in the lungs.
- ANOVA analysis of variance
- mice tolerated the dosing regimen well and there was no effect on body weight throughout the study. There was no sign of laboured breathing at any time and the mice remained outwardly healthy throughout the study. However, on the final day of the study (Day 21), 2 mice were found dead (animal 172 in Group 4 and animal 177 in Group 5). These deaths were not thought to be test substance related.
- HCV hepatitis C virus
- HCV bovine diarrhoea virus
- At least some of the pharmacological activities of the alkaloids of the invention may be based on a secondary glycosidase inhibitory activity.
- Inhibitors of glycosidases particularly those blocking ⁇ -glucosidases and ⁇ - mannosidase, have been shown to prevent replication of several enveloped viruses.
- Such inhibitors may act by interfering with the folding of the viral envelope glycoprotein, so preventing the initial virus-host cell interaction or subsequent fusion. They may also prevent viral duplication by preventing the construction of the proper glycoprotein required for the completion of the viral membrane.
- alkaloids of the invention to exert a direct anti-BVDV effect In vitro was therefore tested and activity demonstrated in a BVDV plaque inhibition assay.
- MDBK cells were seeded in 24 well plates and allowed to reach confiuency. Monolayers were exposed to between 14 and 45 plaque forming units of BVDV and adsorption allowed to take place. Infected monolayers were then exposed to the test compounds, medium added containing low gelling-point agarose and allowed to set. The plates were then incubated for 4 days post infection, fixed in 5% formalin and stained with 0.5% neutral red after removal of the agarose layer. Anti-viral activity was measured by determination of plaque inhibition.
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WO2007010266A1 (en) * | 2005-07-20 | 2007-01-25 | Mnl Pharma Limited | Pyrrolidine compositions |
WO2008009894A2 (en) * | 2006-07-15 | 2008-01-24 | Summit Corporation Plc | Use of imino sugars in immunotherapy |
WO2009066069A1 (en) * | 2007-11-21 | 2009-05-28 | Summit Corporation Plc | Treatment of protein folding disorders |
WO2008134265A3 (en) * | 2007-04-24 | 2009-07-30 | Univ Oxford | Iminosugar for treatment of tumors |
WO2011080271A3 (en) * | 2009-12-28 | 2012-01-05 | Centre De Recerca En Salut Internacional De Barcelona | Exosomes derived from reticulocytes infected with plasmodium sp., method for obtaining them and uses thereof |
CN102786562A (en) * | 2012-08-16 | 2012-11-21 | 西南交通大学 | Pyrrolizidine alkaloids and purpose thereof |
US8445670B2 (en) | 2005-09-02 | 2013-05-21 | United Therapeutics Corporation | Combinatorial library approach to iminocyclitols with biological activity |
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TR201906326T4 (en) * | 2010-04-30 | 2019-05-21 | Allovate Llc | Toothpaste for allergic desensitization with oral mucosa. |
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WO2000037465A2 (en) * | 1998-12-18 | 2000-06-29 | Glycodesign Inc. | Novel derivatives of swainsonine, processes for their preparation, and their use as therapeutic agents |
US20030152550A1 (en) * | 2001-07-31 | 2003-08-14 | Francesca Granucci | Dendritic cells and the uses thereof in screening cellular targets and potential drugs |
WO2004064715A2 (en) * | 2003-01-23 | 2004-08-05 | M N L Pharma Limited | Polyhydroxylated pyrrolizidine |
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2005
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- 2005-01-21 US US10/597,290 patent/US20090117083A1/en not_active Abandoned
- 2005-01-21 CA CA002553854A patent/CA2553854A1/en not_active Abandoned
- 2005-01-21 EP EP05701978A patent/EP1711176A1/en not_active Withdrawn
- 2005-01-21 WO PCT/GB2005/000215 patent/WO2005070418A1/en active Application Filing
- 2005-01-21 JP JP2006550281A patent/JP2007518785A/en active Pending
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Also Published As
Publication number | Publication date |
---|---|
AU2005205962B2 (en) | 2010-08-12 |
AU2005205962A1 (en) | 2005-08-04 |
CA2553854A1 (en) | 2005-08-04 |
JP2007518785A (en) | 2007-07-12 |
US20090117083A1 (en) | 2009-05-07 |
GB0401238D0 (en) | 2004-02-25 |
EP1711176A1 (en) | 2006-10-18 |
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