WO2005063980A8 - METHOD OF ENZYMATICALLY CONSTRUCTING RNAi LIBRARY - Google Patents

METHOD OF ENZYMATICALLY CONSTRUCTING RNAi LIBRARY

Info

Publication number
WO2005063980A8
WO2005063980A8 PCT/JP2004/019612 JP2004019612W WO2005063980A8 WO 2005063980 A8 WO2005063980 A8 WO 2005063980A8 JP 2004019612 W JP2004019612 W JP 2004019612W WO 2005063980 A8 WO2005063980 A8 WO 2005063980A8
Authority
WO
WIPO (PCT)
Prior art keywords
enzymatically
target dna
library
rnai library
intended
Prior art date
Application number
PCT/JP2004/019612
Other languages
French (fr)
Japanese (ja)
Other versions
WO2005063980A1 (en
Inventor
Kenzo Hirose
Daisuke Shirane
Kohtaroh Sugao
Shigeyuki Namiki
Masamitsu Iino
Original Assignee
Toudai Tlo Ltd
Kenzo Hirose
Daisuke Shirane
Kohtaroh Sugao
Shigeyuki Namiki
Masamitsu Iino
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Toudai Tlo Ltd, Kenzo Hirose, Daisuke Shirane, Kohtaroh Sugao, Shigeyuki Namiki, Masamitsu Iino filed Critical Toudai Tlo Ltd
Priority to JP2005516704A priority Critical patent/JP4747245B2/en
Publication of WO2005063980A1 publication Critical patent/WO2005063980A1/en
Publication of WO2005063980A8 publication Critical patent/WO2005063980A8/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/30—Production chemically synthesised

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

It is intended to provide a method of enzymatically preparing a systematic RNAi library from a target DNA. According to this method, a target DNA can be prepared not only from the cDNA or genomic sequence of a specific gene but also from a cDNA library. It is also intended to provide a screening method whereby a clone carrying an iRNA expression construct having a desired silencing activity can be selected from an RNAi library. In this screening, more efficient selection can be made by fusing a reporter gene or a negative selection marker with a target DNA.
PCT/JP2004/019612 2003-12-31 2004-12-28 METHOD OF ENZYMATICALLY CONSTRUCTING RNAi LIBRARY WO2005063980A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2005516704A JP4747245B2 (en) 2003-12-31 2004-12-28 Enzymatic construction method of RNAi library

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US53385403P 2003-12-31 2003-12-31
US60/533,854 2003-12-31

Publications (2)

Publication Number Publication Date
WO2005063980A1 WO2005063980A1 (en) 2005-07-14
WO2005063980A8 true WO2005063980A8 (en) 2006-02-09

Family

ID=34738877

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2004/019612 WO2005063980A1 (en) 2003-12-31 2004-12-28 METHOD OF ENZYMATICALLY CONSTRUCTING RNAi LIBRARY

Country Status (2)

Country Link
JP (1) JP4747245B2 (en)
WO (1) WO2005063980A1 (en)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6254564B1 (en) 1998-09-10 2001-07-03 Percardia, Inc. Left ventricular conduit with blood vessel graft
DE10010073B4 (en) 2000-02-28 2005-12-22 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Anchoring for implantable heart valve prostheses
FR2828263B1 (en) 2001-08-03 2007-05-11 Philipp Bonhoeffer DEVICE FOR IMPLANTATION OF AN IMPLANT AND METHOD FOR IMPLANTATION OF THE DEVICE
DE102005003632A1 (en) 2005-01-20 2006-08-17 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Catheter for the transvascular implantation of heart valve prostheses
DE102005051849B4 (en) 2005-10-28 2010-01-21 JenaValve Technology Inc., Wilmington Device for implantation and attachment of heart valve prostheses
DE102005052628B4 (en) 2005-11-04 2014-06-05 Jenavalve Technology Inc. Self-expanding, flexible wire mesh with integrated valvular prosthesis for the transvascular heart valve replacement and a system with such a device and a delivery catheter
JP2007129934A (en) * 2005-11-09 2007-05-31 Tokyo Metropolitan Univ Method for identifying genome sequence adjacent to gene search vector and variant susceptible to oxidative stress
GB0706631D0 (en) * 2007-04-04 2007-05-16 King S College London Nucleic acids and libraries
US9138315B2 (en) 2007-04-13 2015-09-22 Jenavalve Technology Gmbh Medical device for treating a heart valve insufficiency or stenosis
US7896915B2 (en) 2007-04-13 2011-03-01 Jenavalve Technology, Inc. Medical device for treating a heart valve insufficiency
CN101720211B (en) 2007-05-15 2013-06-05 耶拿阀门科技公司 Handle for manipulating a catheter tip, catheter system and medical insertion system for inserting a self-expandable heart valve stent
US9168130B2 (en) 2008-02-26 2015-10-27 Jenavalve Technology Gmbh Stent for the positioning and anchoring of a valvular prosthesis in an implantation site in the heart of a patient
WO2011104269A1 (en) 2008-02-26 2011-09-01 Jenavalve Technology Inc. Stent for the positioning and anchoring of a valvular prosthesis in an implantation site in the heart of a patient
US9044318B2 (en) 2008-02-26 2015-06-02 Jenavalve Technology Gmbh Stent for the positioning and anchoring of a valvular prosthesis
US8465540B2 (en) 2008-02-26 2013-06-18 Jenavalve Technology, Inc. Stent for the positioning and anchoring of a valvular prosthesis
US8398704B2 (en) 2008-02-26 2013-03-19 Jenavalve Technology, Inc. Stent for the positioning and anchoring of a valvular prosthesis in an implantation site in the heart of a patient
WO2010071206A1 (en) * 2008-12-18 2010-06-24 国立大学法人名古屋大学 DNA CONSTRUCT FOR TRANSCRIPTION/EXPRESSION OF siRNA, AND USE THEREOF
US11278406B2 (en) 2010-05-20 2022-03-22 Jenavalve Technology, Inc. Catheter system for introducing an expandable heart valve stent into the body of a patient, insertion system with a catheter system and medical device for treatment of a heart valve defect
JP2013526388A (en) 2010-05-25 2013-06-24 イエナバルブ テクノロジー インク Artificial heart valve, and transcatheter delivery prosthesis comprising an artificial heart valve and a stent
EP2768432A1 (en) 2011-10-21 2014-08-27 JenaValve Technology Inc. Catheter system for introducing an expandable heart valve stent into the body of a patient, insertion system with a catheter system and medical device for treatment of a heart valve defect
ES2665071T3 (en) 2012-02-17 2018-04-24 Fred Hutchinson Cancer Research Center Compositions and methods to identify mutations accurately
CN104470471B (en) 2012-05-16 2017-05-31 耶拿阀门科技有限责任公司 Catheter delivery system and Medical Devices for treating cardiac valves defect for introducing distensible heart valve prosthesis
JP6563394B2 (en) 2013-08-30 2019-08-21 イェーナヴァルヴ テクノロジー インコーポレイテッド Radially foldable frame for an artificial valve and method for manufacturing the frame
JP6256911B2 (en) * 2013-10-31 2018-01-10 国立研究開発法人産業技術総合研究所 New counter selection method
EP3632378B1 (en) 2015-05-01 2024-05-29 JenaValve Technology, Inc. Device with reduced pacemaker rate in heart valve replacement
CN109475419B (en) 2016-05-13 2021-11-09 耶拿阀门科技股份有限公司 Heart valve prosthesis delivery systems and methods for delivering heart valve prostheses through guide sheaths and loading systems
JP7094965B2 (en) 2017-01-27 2022-07-04 イエナバルブ テクノロジー インク Heart valve imitation
WO2018183942A1 (en) * 2017-03-31 2018-10-04 Grail, Inc. Improved library preparation and use thereof for sequencing-based error correction and/or variant identification

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1474433A4 (en) * 2002-02-20 2005-02-23 Sirna Therapeutics Inc RNA INTERFERENCE MEDIATED TARGET DISCOVERY AND TARGET VALIDATION USING SHORT INTERFERING NUCLEIC ACID (siNA)
JP2005529624A (en) * 2002-06-21 2005-10-06 シノゲノマックス カンパニー リミテッド Random DNA library and double-stranded RNA library, its use and production method
EP1554386A2 (en) * 2002-07-24 2005-07-20 Immusol Incorporated Single promoter system for making sirna expression cassettes and expression libraries using a polymerase primer hairpin linker
AU2004210972A1 (en) * 2003-02-11 2004-08-26 Immusol Incorporated siRNA libraries optimized for predetermined protein families
JP2007502129A (en) * 2003-05-09 2007-02-08 ユニヴァーシティ オヴ ピッツバーグ オヴ ザ コモンウェルス システム オヴ ハイアー エデュケーション Short interfering RNA libraries and methods of synthesis and use
AU2004286261B2 (en) * 2003-10-27 2010-06-24 Merck Sharp & Dohme Llc Method of designing siRNAs for gene silencing

Also Published As

Publication number Publication date
WO2005063980A1 (en) 2005-07-14
JPWO2005063980A1 (en) 2007-12-20
JP4747245B2 (en) 2011-08-17

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