WO2005047472A3 - Augmentation de la teneur des grains en threonine par la modification de l'activite d'aldolase threonine - Google Patents

Augmentation de la teneur des grains en threonine par la modification de l'activite d'aldolase threonine Download PDF

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Publication number
WO2005047472A3
WO2005047472A3 PCT/US2004/037369 US2004037369W WO2005047472A3 WO 2005047472 A3 WO2005047472 A3 WO 2005047472A3 US 2004037369 W US2004037369 W US 2004037369W WO 2005047472 A3 WO2005047472 A3 WO 2005047472A3
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WO
WIPO (PCT)
Prior art keywords
threonine
nucleic acid
disclosed
present
alteration
Prior art date
Application number
PCT/US2004/037369
Other languages
English (en)
Other versions
WO2005047472A2 (fr
Inventor
Georg Jander
Vijay Joshi
Original Assignee
Thompson Boyce Plant Res
Georg Jander
Vijay Joshi
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Thompson Boyce Plant Res, Georg Jander, Vijay Joshi filed Critical Thompson Boyce Plant Res
Publication of WO2005047472A2 publication Critical patent/WO2005047472A2/fr
Publication of WO2005047472A3 publication Critical patent/WO2005047472A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1014—Hydroxymethyl-, formyl-transferases (2.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

La présente invention concerne un structure d'acide nucléique dans laquelle une molécule d'acide nucléique est configurée pour le silençage de l'expression d'aldolase thréonine. La présente invention concerne aussi une structure contenant une molécule d'acide nucléique codant une aldolase thréonine et une séquence de ciblage mitochondriale. L'invention porte aussi sur des vecteurs d'expression, des cellules hôtes, des plantes et des grains des plantes transformées avec des structures d'acide nucléique de la présente invention. L'invention concerne aussi des aliments pour animaux ou pour humains contenant des grains de plantes transformées. L'invention concerne des procédés pour augmenter la teneur des plantes en thréonine et en isoleucine, ainsi qu'un procédé pour produire une plante mutante présentant des taux plus élevés de thréonine dans les grains en comparaison à un plante non mutante.
PCT/US2004/037369 2003-11-10 2004-11-12 Augmentation de la teneur des grains en threonine par la modification de l'activite d'aldolase threonine WO2005047472A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US51931303P 2003-11-10 2003-11-10
US60/519,313 2003-11-10

Publications (2)

Publication Number Publication Date
WO2005047472A2 WO2005047472A2 (fr) 2005-05-26
WO2005047472A3 true WO2005047472A3 (fr) 2006-05-26

Family

ID=34590390

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/037369 WO2005047472A2 (fr) 2003-11-10 2004-11-12 Augmentation de la teneur des grains en threonine par la modification de l'activite d'aldolase threonine

Country Status (2)

Country Link
AR (1) AR046836A1 (fr)
WO (1) WO2005047472A2 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103468792B (zh) * 2013-07-11 2015-11-25 江西省农业科学院水稻研究所 水稻双t-dna转基因非连锁整合的分子标记检测方法
CN107012162B (zh) * 2016-01-28 2020-12-08 中国种子集团有限公司 农杆菌介导的棉花胚尖快速转化方法
JP7308870B2 (ja) 2016-08-22 2023-07-14 日本たばこ産業株式会社 乾燥たばこ材料の生産方法

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
UY37482A (es) * 2016-11-16 2018-05-31 Cellectis Métodos para alterar el contenido de aminoácidos en plantas mediante mutaciones de desplazamiento de marco
CN110272911A (zh) * 2019-07-05 2019-09-24 四川大学 AOX1a基因在提高植物耐旱性方面的应用
CN115109769B (zh) * 2022-05-20 2023-07-14 华东理工大学 一种l-苏氨酸醛缩酶突变体以及在l-丝氨酸合成中的应用
CN117264976B (zh) * 2023-09-19 2024-03-19 河北省农林科学院棉花研究所（河北省农林科学院特种经济作物研究所） 一种棉花细胞壁强度调控基因及其应用

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
JANDER G. ET AL: "Application of a High-Throughput HPLC-MS/MS Assay to Arabidopsis Mutant Screening; Evidence that Threonine Aldolase Plays a Role in Seed Nutritional Quality.", PLANT J., vol. 39, no. 3, August 2004 (2004-08-01), pages 465 - 475, XP002992770 *
LEE S.J. ET AL: "High-Throughput Screening Methods for Selecting L-Threonine Adolases with Improved Activity.", J MOL CATAL B: ENZY., vol. 26, no. 3-6, 2003, pages 265 - 272, XP002992768 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103468792B (zh) * 2013-07-11 2015-11-25 江西省农业科学院水稻研究所 水稻双t-dna转基因非连锁整合的分子标记检测方法
CN107012162B (zh) * 2016-01-28 2020-12-08 中国种子集团有限公司 农杆菌介导的棉花胚尖快速转化方法
JP7308870B2 (ja) 2016-08-22 2023-07-14 日本たばこ産業株式会社 乾燥たばこ材料の生産方法

Also Published As

Publication number Publication date
AR046836A1 (es) 2005-12-28
WO2005047472A2 (fr) 2005-05-26

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