WO2005044845A3 - Protein binding miniature proteins - Google Patents

Protein binding miniature proteins Download PDF

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Publication number
WO2005044845A3
WO2005044845A3 PCT/US2004/037210 US2004037210W WO2005044845A3 WO 2005044845 A3 WO2005044845 A3 WO 2005044845A3 US 2004037210 W US2004037210 W US 2004037210W WO 2005044845 A3 WO2005044845 A3 WO 2005044845A3
Authority
WO
WIPO (PCT)
Prior art keywords
protein binding
miniature proteins
polypeptide
binding miniature
proteins
Prior art date
Application number
PCT/US2004/037210
Other languages
French (fr)
Other versions
WO2005044845A2 (en
Inventor
Alanna S Schepartz
Jason W K Chin
Reena Zutshi
Stacey E Rutledge
Martin Joanne D Kehlbeck
Neal J Zondlo
Original Assignee
Univ Yale
Alanna S Schepartz
Jason W K Chin
Reena Zutshi
Stacey E Rutledge
Martin Joanne D Kehlbeck
Neal J Zondlo
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Yale, Alanna S Schepartz, Jason W K Chin, Reena Zutshi, Stacey E Rutledge, Martin Joanne D Kehlbeck, Neal J Zondlo filed Critical Univ Yale
Priority to GB0609747A priority Critical patent/GB2422836A/en
Priority to CA002544764A priority patent/CA2544764A1/en
Publication of WO2005044845A2 publication Critical patent/WO2005044845A2/en
Publication of WO2005044845A3 publication Critical patent/WO2005044845A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/465—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from birds
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/02—Preparation of hybrid cells by fusion of two or more cells, e.g. protoplast fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Abstract

The present invention provides a protein scaffold, such as an avian pancreatic polypeptide, that can be modified by substitution of two or more amino acid residues that are exposed on the alpha helix domain of the polypeptide when the polypeptide is in a tertiary form.
PCT/US2004/037210 2003-11-04 2004-11-04 Protein binding miniature proteins WO2005044845A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
GB0609747A GB2422836A (en) 2003-11-04 2004-11-04 Protein binding miniature proteins
CA002544764A CA2544764A1 (en) 2003-11-04 2004-11-04 Protein binding miniature proteins

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US51749603P 2003-11-04 2003-11-04
US60/517,496 2003-11-04

Publications (2)

Publication Number Publication Date
WO2005044845A2 WO2005044845A2 (en) 2005-05-19
WO2005044845A3 true WO2005044845A3 (en) 2006-03-02

Family

ID=34572947

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/037210 WO2005044845A2 (en) 2003-11-04 2004-11-04 Protein binding miniature proteins

Country Status (3)

Country Link
CA (1) CA2544764A1 (en)
GB (1) GB2422836A (en)
WO (1) WO2005044845A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7232533B2 (en) 2014-05-21 2023-03-03 プレジデント アンド フェローズ オブ ハーバード カレッジ RAS inhibitory peptides and uses thereof

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19932688B4 (en) 1999-07-13 2009-10-08 Scil Proteins Gmbh Design of beta-sheet proteins of gamma-II-crystalline antibody-like
DE10324447A1 (en) 2003-05-28 2004-12-30 Scil Proteins Gmbh Generation of artificial binding proteins based on ubiquitin
EP1925664A1 (en) * 2006-11-15 2008-05-28 Scil proteins GmbH Artificial binding proteins based on a modified alpha helical region of ubiquitin
CN102753568B (en) 2009-12-14 2014-08-20 塞尔蛋白质股份有限公司 A method for identifying hetero-multimeric modified ubiquitin proteins with binding capability to ligands
CA2837804C (en) 2011-06-15 2018-03-20 Scil Proteins Gmbh Dimeric binding proteins based on modified ubiquitins
EP3253785B1 (en) 2015-02-06 2019-04-10 Navigo Proteins Gmbh Novel egfr binding proteins
AU2016293063B2 (en) 2015-07-16 2018-11-08 Navigo Proteins Gmbh Novel immunoglobulin-binding proteins and their use in affinity purification
JP2018520675A (en) 2015-07-20 2018-08-02 ナフィゴ プロテインズ ゲゼルシャフト ミット ベシュレンクテル ハフツングＮａｖｉｇｏ Ｐｒｏｔｅｉｎｓ ＧｍｂＨ Novel binding protein based on diubiquitin mutant protein and production method thereof
CN109310780A (en) 2016-05-04 2019-02-05 纳维格蛋白质有限公司 The target compound being coupled for chemical part site-specificity comprising peptide linker
CN116333065A (en) 2016-08-11 2023-06-27 纳维格蛋白质有限公司 Novel alkali-stable immunoglobulin-binding proteins
EP3706804B1 (en) 2017-11-07 2022-02-23 Navigo Proteins GmbH Fusion proteins with specificity for ed-b and long serum half-life for diagnosis or treatment of cancer

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001081375A2 (en) * 2000-04-24 2001-11-01 Yale University Dna & protein binding miniature proteins
WO2003053996A2 (en) * 2001-12-20 2003-07-03 Kimberly-Clark Worldwide, Inc. Modular peptide-based reagent

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001081375A2 (en) * 2000-04-24 2001-11-01 Yale University Dna & protein binding miniature proteins
US20030166240A1 (en) * 2000-04-24 2003-09-04 Yale University DNA & protein binding miniature proteins
WO2003053996A2 (en) * 2001-12-20 2003-07-03 Kimberly-Clark Worldwide, Inc. Modular peptide-based reagent

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
CHIN JASON W ET AL: "Methodology for optimizing functional miniature proteins based on avian pancreatic polypeptide using phage display", BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, OXFORD, GB, vol. 11, no. 12, 18 June 2001 (2001-06-18), pages 1501 - 1505, XP002196761, ISSN: 0960-894X *
CHIN JW AND SCHEPARTZ A: "Design and Evolution of a Miniature BCL-2 binding protein", ANGEWANDTE CHEMIE. INTERNATIONAL EDITION, WILEY VCH VERLAG, WEINHEIM, DE, vol. 40, no. 20, 2001, pages 3806 - 3809, XP008020924, ISSN: 1433-7851 *
CUNNINGHAM B C ET AL: "MINIMIZED PROTEINS", CURRENT OPINION IN STRUCTURAL BIOLOGY, CURRENT BIOLOGY LTD., LONDON, GB, vol. 7, no. 4, August 1997 (1997-08-01), pages 457 - 462, XP001026709, ISSN: 0959-440X *
RUTLEDGE S E ET AL: "DESIGN AND SELECTION OF PROTEIN-BINDING MINIATURE PROTEINS", AMERICAN CHEMICAL SOCIETY. ABSTRACTS OF PAPER. AT THE NATIONAL MEETING, AMERICAN CHEMICAL SOCIETY, WASHINGTON, DC, US, vol. 224, no. 1/2, 18 August 2002 (2002-08-18), pages ABSTRNOMEDI - 197, XP008047429, ISSN: 0065-7727 *
RUTLEDGE S E ET AL: "Molecular recognition of protein surfaces: high affinity ligands for the CBP KIX domain", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, AMERICAN CHEMICAL SOCIETY, WASHINGTON, DC, US, vol. 125, no. 47, 26 November 2003 (2003-11-26), pages 14336 - 14347, XP002331700, ISSN: 0002-7863 *
SCHEPARTZ A: "DESIGN AND DISCOVERY OF FUNCTIONAL MINIATURE PROTEINS", AMERICAN CHEMICAL SOCIETY. ABSTRACTS OF PAPER. AT THE NATIONAL MEETING, AMERICAN CHEMICAL SOCIETY, WASHINGTON, DC, US, vol. 223, no. 1/2, 7 April 2002 (2002-04-07), pages ABSTRNOORGN - 136, XP008047433, ISSN: 0065-7727 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7232533B2 (en) 2014-05-21 2023-03-03 プレジデント アンド フェローズ オブ ハーバード カレッジ RAS inhibitory peptides and uses thereof

Also Published As

Publication number Publication date
GB0609747D0 (en) 2006-06-28
CA2544764A1 (en) 2005-05-19
WO2005044845A2 (en) 2005-05-19
GB2422836A (en) 2006-08-09

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