GB2422836A - Protein binding miniature proteins - Google Patents

Protein binding miniature proteins Download PDF

Info

Publication number
GB2422836A
GB2422836A GB0609747A GB0609747A GB2422836A GB 2422836 A GB2422836 A GB 2422836A GB 0609747 A GB0609747 A GB 0609747A GB 0609747 A GB0609747 A GB 0609747A GB 2422836 A GB2422836 A GB 2422836A
Authority
GB
United Kingdom
Prior art keywords
protein binding
miniature proteins
polypeptide
binding miniature
proteins
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
GB0609747A
Other versions
GB0609747D0 (en
Inventor
Alanna S Schepartz
Jason W K Chin
Reena Zutshi
Stacey E Rutledge
Joanne D Kehlbeck Martin
Neal J Zondlo
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Yale University
Original Assignee
Yale University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yale University filed Critical Yale University
Publication of GB0609747D0 publication Critical patent/GB0609747D0/en
Publication of GB2422836A publication Critical patent/GB2422836A/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/465—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from birds
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/02—Preparation of hybrid cells by fusion of two or more cells, e.g. protoplast fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention provides a protein scaffold, such as an avian pancreatic polypeptide, that can be modified by substitution of two or more amino acid residues that are exposed on the alpha helix domain of the polypeptide when the polypeptide is in a tertiary form.

Description

GB 2422836 A continuation
(72) cont (58) Field of Search by ISA:
Stacey E Rutledge NT CL c07K, C12N Joanne D Kehlbeck Martin Other: EPOInternal, WPI, BIOSIS, MEDLINE, EM BASE, Neal J Zondlo EM BL (74) Agent and/or Address for Service: Barker Brettell 138 Hagley Road, Edgbaston, BIRMINGHAM, B16 9PW, United Kingdom
GB0609747A 2003-11-04 2004-11-04 Protein binding miniature proteins Withdrawn GB2422836A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US51749603P 2003-11-04 2003-11-04
PCT/US2004/037210 WO2005044845A2 (en) 2003-11-04 2004-11-04 Protein binding miniature proteins

Publications (2)

Publication Number Publication Date
GB0609747D0 GB0609747D0 (en) 2006-06-28
GB2422836A true GB2422836A (en) 2006-08-09

Family

ID=34572947

Family Applications (1)

Application Number Title Priority Date Filing Date
GB0609747A Withdrawn GB2422836A (en) 2003-11-04 2004-11-04 Protein binding miniature proteins

Country Status (3)

Country Link
CA (1) CA2544764A1 (en)
GB (1) GB2422836A (en)
WO (1) WO2005044845A2 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19932688B4 (en) 1999-07-13 2009-10-08 Scil Proteins Gmbh Design of beta-sheet proteins of gamma-II-crystalline antibody-like
DE10324447A1 (en) 2003-05-28 2004-12-30 Scil Proteins Gmbh Generation of artificial binding proteins based on ubiquitin
EP1925664A1 (en) 2006-11-15 2008-05-28 Scil proteins GmbH Artificial binding proteins based on a modified alpha helical region of ubiquitin
EP2379581B1 (en) 2009-12-14 2013-10-09 Scil Proteins GmbH A method for identifying hetero-multimeric modified ubiquitin proteins with binding capability to ligands
AU2012268970B2 (en) 2011-06-15 2016-01-28 Navigo Proteins Gmbh Dimeric binding proteins based on modified ubiquitins
EP3145523A4 (en) 2014-05-21 2018-02-21 President and Fellows of Harvard College Ras inhibitory peptides and uses thereof
EP3253785B1 (en) 2015-02-06 2019-04-10 Navigo Proteins Gmbh Novel egfr binding proteins
WO2017009421A1 (en) 2015-07-16 2017-01-19 Scil Proteins Gmbh Novel immunoglobulin-binding proteins and their use in affinity purification
JP2018520675A (en) 2015-07-20 2018-08-02 ナフィゴ プロテインズ ゲゼルシャフト ミット ベシュレンクテル ハフツングＮａｖｉｇｏ Ｐｒｏｔｅｉｎｓ ＧｍｂＨ Novel binding protein based on diubiquitin mutant protein and production method thereof
US11813336B2 (en) 2016-05-04 2023-11-14 Navigo Proteins Gmbh Targeted compounds for the site-specific coupling of chemical moieties comprising a peptide linker
JP7181612B2 (en) 2016-08-11 2022-12-01 ナフィゴ プロテインズ ゲゼルシャフト ミット ベシュレンクテル ハフツング alkali-stable immunoglobulin-binding protein
EP3706804B1 (en) 2017-11-07 2022-02-23 Navigo Proteins GmbH Fusion proteins with specificity for ed-b and long serum half-life for diagnosis or treatment of cancer

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001081375A2 (en) * 2000-04-24 2001-11-01 Yale University Dna & protein binding miniature proteins
WO2003053996A2 (en) * 2001-12-20 2003-07-03 Kimberly-Clark Worldwide, Inc. Modular peptide-based reagent

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001081375A2 (en) * 2000-04-24 2001-11-01 Yale University Dna & protein binding miniature proteins
US20030166240A1 (en) * 2000-04-24 2003-09-04 Yale University DNA & protein binding miniature proteins
WO2003053996A2 (en) * 2001-12-20 2003-07-03 Kimberly-Clark Worldwide, Inc. Modular peptide-based reagent

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
Am. Chem. Soc. Abs., Vol.223, 2002, Schepartz, A., "Designa nd discovery of...", p.Abstrnoorgn 136 *
Am. Chem. Soc. Abs., Vol.224, 2002, Rutledge, S. E. et al., "Design and selection...", pp.Abstrnomedi-197 *
Angewandte Chemie, Vol.40, 2001, Chin, J. W. et al., "Design and evolution of...", pp.3806-3809 *
Bioorg. Med. Chem. Letts., Vol.11, 2001, Chin, J. et al., "Methodology for optimising...", pp.1501-1505 *
Curr. Opin. Struct. Biol., Vol.7, 1997, Cunningham, B. C. et al., "Minimized proteins", pp.457-462 *
J. Am. Chem. Soc., Vol.125, 2003, Rutledge, S. E> et al., "Molecular recognition of...", pp.14336-14347 *

Also Published As

Publication number Publication date
WO2005044845A2 (en) 2005-05-19
WO2005044845A3 (en) 2006-03-02
GB0609747D0 (en) 2006-06-28
CA2544764A1 (en) 2005-05-19

Similar Documents

Publication Publication Date Title
GB2422836A (en) Protein binding miniature proteins
DE602005020165D1 (en) PROCESS FOR THE PREPARATION OF L-ARGININE, L-ORNITHIN OR L-CITRULLINE
RS50966B (en) Pyy agonists and uses thereof
EP1939293A3 (en) Interferon-Like Protein ZCYTO21
EP2290057A3 (en) Variant lipolytic enzymes
WO2003079972A3 (en) Active agent delivery systems and methods for protecting and administering active agents
DE60113381D1 (en) AUTOMATION IN PROTEIN DICE BY PROTEIN LIBRARIES
BRPI0514411A (en) formulation, and method of producing a formulation
AU2003231471A1 (en) Antimicrobial polypeptide and utizliation thereof
WO2007029262A3 (en) Compositions and methods using same for the detection of viruses
WO1999060013A3 (en) Il-6 antagonist peptides
BG104267A (en) Aminoterminally incised rantes as haemokin antagonists
WO2003010336A3 (en) Molecular markers for hepatocellular carcinoma
WO2001081375A3 (en) Dna & protein binding miniature proteins
WO2005059131A3 (en) Protein binding miniature proteins and uses thereof
WO2021072129A8 (en) Proteins containing multiple, different unnatural amino acids and methods of making and using such proteins
WO2002062304A3 (en) Cosmetic and/or pharmaceutical agents containing an acylated amino acid and a protein condensate
SE0301431D0 (en) Novel antimicrobial peptides
BRPI0414157A (en) isolated polypeptide, isolated nucleic acid, method for inhibiting white spot syndrome virus infection of a crustacean, and, food for a crustacean
GB0010314D0 (en) Anti-freeze proteins their production and use
ATE435870T1 (en) PEPTIDE INHIBITORS OF TOXINS DERIVED FROM LL-37
WO2007058267A8 (en) Novel protein and gene encoding the protein
WO2006029343A3 (en) Peptides that selectively home to heart vasculature and related conjugates and methods
WO2003091275A3 (en) Compositions containing anti-hiv peptides and methods of use
WO2004087912A8 (en) Inhibitor proteins of a protease and use thereof

Legal Events

Date Code Title Description
WAP Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1)