WO2005014786A3 - Enhanced engineered chromosome formation from alpha satellite with artificially increased density of cenp-b boxes - Google Patents

Enhanced engineered chromosome formation from alpha satellite with artificially increased density of cenp-b boxes Download PDF

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Publication number
WO2005014786A3
WO2005014786A3 PCT/US2004/022314 US2004022314W WO2005014786A3 WO 2005014786 A3 WO2005014786 A3 WO 2005014786A3 US 2004022314 W US2004022314 W US 2004022314W WO 2005014786 A3 WO2005014786 A3 WO 2005014786A3
Authority
WO
WIPO (PCT)
Prior art keywords
cenp
boxes
increased density
artificially increased
chromosome formation
Prior art date
Application number
PCT/US2004/022314
Other languages
French (fr)
Other versions
WO2005014786A2 (en
Inventor
Bokkelen Gil B Van
Joydeep Basu
Original Assignee
Athersys Inc
Bokkelen Gil B Van
Joydeep Basu
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Athersys Inc, Bokkelen Gil B Van, Joydeep Basu filed Critical Athersys Inc
Publication of WO2005014786A2 publication Critical patent/WO2005014786A2/en
Publication of WO2005014786A3 publication Critical patent/WO2005014786A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/20—Pseudochromosomes, minichrosomosomes
    • C12N2800/204—Pseudochromosomes, minichrosomosomes of bacterial origin, e.g. BAC
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/20—Pseudochromosomes, minichrosomosomes
    • C12N2800/208—Pseudochromosomes, minichrosomosomes of mammalian origin, e.g. minichromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/90—Vectors containing a transposable element

Abstract

The invention relates to the discovery that the distribution of CENP-B boxes on the alpha-satellite DNA affects efficiency of the centromere and thereby, affects efficiency of chromosome formation. Therefore, engineered chromosome vectors with altered distribution of CENP-B boxes are described that can alter the rate of chromosome formation as desired. Engineered HORs, chromosome vectors, and chromosomes as well as methods of making the same are described and claimed.
PCT/US2004/022314 2003-08-07 2004-08-09 Enhanced engineered chromosome formation from alpha satellite with artificially increased density of cenp-b boxes WO2005014786A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US49296503P 2003-08-07 2003-08-07
US60/492,965 2003-08-07

Publications (2)

Publication Number Publication Date
WO2005014786A2 WO2005014786A2 (en) 2005-02-17
WO2005014786A3 true WO2005014786A3 (en) 2005-09-22

Family

ID=34135185

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/022314 WO2005014786A2 (en) 2003-08-07 2004-08-09 Enhanced engineered chromosome formation from alpha satellite with artificially increased density of cenp-b boxes

Country Status (1)

Country Link
WO (1) WO2005014786A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9139849B2 (en) 2005-04-08 2015-09-22 The United States of America as Represented by the Government of the Department of Health and Human Services Rapid generation of long synthetic centromeric tandem repeats for mammalian artificial chromosome formation

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6348353B1 (en) * 1995-06-07 2002-02-19 Case Western Reserve University Artificial mammalian chromosome

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6348353B1 (en) * 1995-06-07 2002-02-19 Case Western Reserve University Artificial mammalian chromosome

Also Published As

Publication number Publication date
WO2005014786A2 (en) 2005-02-17

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