WO2005010156A3 - Genes encoding carotenoid compounds - Google Patents

Genes encoding carotenoid compounds Download PDF

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Publication number
WO2005010156A3
WO2005010156A3 PCT/US2004/022747 US2004022747W WO2005010156A3 WO 2005010156 A3 WO2005010156 A3 WO 2005010156A3 US 2004022747 W US2004022747 W US 2004022747W WO 2005010156 A3 WO2005010156 A3 WO 2005010156A3
Authority
WO
WIPO (PCT)
Prior art keywords
cluster
crtx
crtz
crty
crtb
Prior art date
Application number
PCT/US2004/022747
Other languages
French (fr)
Other versions
WO2005010156A2 (en
Inventor
Qiong Cheng
Natalia Sedkova
Luan Tao
Original Assignee
Du Pont
Qiong Cheng
Natalia Sedkova
Luan Tao
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont, Qiong Cheng, Natalia Sedkova, Luan Tao filed Critical Du Pont
Priority to AU2004260061A priority Critical patent/AU2004260061A1/en
Priority to CA002527557A priority patent/CA2527557A1/en
Priority to EP04778321A priority patent/EP1646713A2/en
Publication of WO2005010156A2 publication Critical patent/WO2005010156A2/en
Priority to NO20060783A priority patent/NO20060783L/en
Publication of WO2005010156A3 publication Critical patent/WO2005010156A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083—Miscellaneous (1.14.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y205/00—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
    • C12Y205/01—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
    • C12Y205/01029—Geranylgeranyl diphosphate synthase (2.5.1.29)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y503/00—Intramolecular oxidoreductases (5.3)
    • C12Y503/03—Intramolecular oxidoreductases (5.3) transposing C=C bonds (5.3.3)
    • C12Y503/03002—Isopentenyl-diphosphate DELTA-isomerase (5.3.3.2)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel

Abstract

A carotenogenic biosynthetic gene cluster has been isolated from Panteoa stewartii strain DC413, wherein the genetic organization of the cluster is crtE-idi-crtX-crtY-crtI-crtB-crtZ. The genes contained within this cluster encode geranylgeranyl pyrophosphate (GGPP) synthetase (CrtE), isopentenyl pyrophosphate isomerase (Idi), zeaxanthin glucosyl transferase (CrtX), lycopene cyclase (CrtY), phytoene desaturase (CrtI), phytoene synthase (CrtB), and b-carotene hydroxylase (CrtZ). The gene cluster, genes and their products are useful for the conversion of farnesyl pyrophosphate to carotenoids. Vectors containing those DNA segments, host cells containing the vectors and methods for producing those enzymes by recombinant DNA technology in transformed host organisms are disclosed.
PCT/US2004/022747 2003-07-17 2004-07-14 Genes encoding carotenoid compounds WO2005010156A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU2004260061A AU2004260061A1 (en) 2003-07-17 2004-07-14 Genes encoding carotenoid compounds
CA002527557A CA2527557A1 (en) 2003-07-17 2004-07-14 Genes encoding carotenoid compounds
EP04778321A EP1646713A2 (en) 2003-07-17 2004-07-14 Genes encoding carotenoid compounds
NO20060783A NO20060783L (en) 2003-07-17 2006-02-17 Genes encoding carotenoid compounds

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US48818303P 2003-07-17 2003-07-17
US60/488,183 2003-07-17
US52708303P 2003-12-03 2003-12-03
US60/527,083 2003-12-03

Publications (2)

Publication Number Publication Date
WO2005010156A2 WO2005010156A2 (en) 2005-02-03
WO2005010156A3 true WO2005010156A3 (en) 2007-05-03

Family

ID=34107764

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/022747 WO2005010156A2 (en) 2003-07-17 2004-07-14 Genes encoding carotenoid compounds

Country Status (4)

Country Link
EP (1) EP1646713A2 (en)
AU (1) AU2004260061A1 (en)
CA (1) CA2527557A1 (en)
WO (1) WO2005010156A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8691555B2 (en) 2006-09-28 2014-04-08 Dsm Ip Assests B.V. Production of carotenoids in oleaginous yeast and fungi

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BRPI0609040B1 (en) 2005-03-18 2018-07-31 Microbia, Inc. YARROWIA RECOMBINATING FUNGUS, METHOD FOR CAROTENOID PRODUCTION AND METHOD FOR PREPARING A FOOD OR FOOD CONTAINING A CAROTENOID
WO2018222946A1 (en) * 2017-06-01 2018-12-06 Knipbio, Inc. Heterologous carotenoid production in microorganisms
CN115807026B (en) * 2022-08-01 2023-09-01 深圳大学 Construction method and application of astaxanthin synthesis path in Chlamydomonas reinhardtii
CN117264865B (en) * 2023-11-21 2024-03-12 清华大学 Recombinant rhodococcus erythropolis and application thereof in compound synthesis

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
MISAWA J. ET AL.: "Metabolic engineering for the production of carotenoids in non-carotenogenic bacteria and yeats", JOURNAL OF BIOTECHNOLOGY, vol. 59, 1998, pages 169 - 181, XP004113748 *
NGO ET AL.: "Computational Complexity;Protein Structure Prediction, and the Levinthal Paradox", THE PROTEIN FOLDING PROBLEM & TERTIARY STRUCTURE PREDICTION, 1994, pages 433 - 492-495 *
POTRYKUS I.: "Gene Transfer To Cereals: An Assessment", BIOTECHNOLOGY, vol. 8, no. 6, 1990, pages 535 - 542 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8691555B2 (en) 2006-09-28 2014-04-08 Dsm Ip Assests B.V. Production of carotenoids in oleaginous yeast and fungi
US9297031B2 (en) 2006-09-28 2016-03-29 Dsm Ip Assets B.V. Production of carotenoids in oleaginous yeast and fungi

Also Published As

Publication number Publication date
CA2527557A1 (en) 2005-02-03
WO2005010156A2 (en) 2005-02-03
EP1646713A2 (en) 2006-04-19
AU2004260061A1 (en) 2005-02-03

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