WO2005007886A3 - Method for displaying and selecting a protease molecule - Google Patents

Method for displaying and selecting a protease molecule Download PDF

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Publication number
WO2005007886A3
WO2005007886A3 PCT/GB2004/003055 GB2004003055W WO2005007886A3 WO 2005007886 A3 WO2005007886 A3 WO 2005007886A3 GB 2004003055 W GB2004003055 W GB 2004003055W WO 2005007886 A3 WO2005007886 A3 WO 2005007886A3
Authority
WO
WIPO (PCT)
Prior art keywords
protease
selecting
mrna
displaying
complexes
Prior art date
Application number
PCT/GB2004/003055
Other languages
French (fr)
Other versions
WO2005007886A2 (en
Inventor
Christopher Scott
Brian Walker
Original Assignee
Univ Belfast
Christopher Scott
Brian Walker
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Belfast, Christopher Scott, Brian Walker filed Critical Univ Belfast
Publication of WO2005007886A2 publication Critical patent/WO2005007886A2/en
Publication of WO2005007886A3 publication Critical patent/WO2005007886A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Described is a method of displaying and selecting a protease molecule, the method comprising: (a) providing mRNA molecules, each mRNA molecule comprising a nucleotide sequence encoding a peptide; (b) incubating the mRNA molecules under conditions suitable for translation of the mRNA molecules to form complexes comprising ribosome, mRNA and encoded nascent peptide displayed on the ribosome; (c) contacting the complexes with a protease binding ligand under suitable binding conditions (i.e. for specific binding of a protease to a protease binding ligand), and selecting one or more complexes which are specifically bound to the protease binding ligand. Also described are methods of identifying proteases.
PCT/GB2004/003055 2003-07-12 2004-07-12 Method for displaying and selecting a protease molecule WO2005007886A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0316314.4 2003-07-12
GBGB0316314.4A GB0316314D0 (en) 2003-07-12 2003-07-12 Method

Publications (2)

Publication Number Publication Date
WO2005007886A2 WO2005007886A2 (en) 2005-01-27
WO2005007886A3 true WO2005007886A3 (en) 2005-04-28

Family

ID=27742036

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2004/003055 WO2005007886A2 (en) 2003-07-12 2004-07-12 Method for displaying and selecting a protease molecule

Country Status (2)

Country Link
GB (1) GB0316314D0 (en)
WO (1) WO2005007886A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016044328A1 (en) * 2014-09-18 2016-03-24 The Regents Of The University Of California Single-molecule phenotype analysis

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5436131A (en) * 1993-04-02 1995-07-25 Merck & Co., Inc. Color screening assay for identifying inhibitor resistant HIV protease mutants
WO1998048008A1 (en) * 1997-04-23 1998-10-29 Plueckthun Andreas Methods for identifying nucleic acid molecules encoding (poly)peptides that interact with target molecules
WO1998054312A1 (en) * 1997-05-28 1998-12-03 Babraham Institute Ribosome complexes as selection particles for in vitro display and evolution of proteins
WO1999011777A1 (en) * 1997-09-03 1999-03-11 Biovation Limited Methods for protein screening
US5952307A (en) * 1994-01-21 1999-09-14 Georgia Tech Research Corp. Basic α-aminoalkylphosphonate derivatives
US6063562A (en) * 1994-09-16 2000-05-16 Sepracor, Inc. In vitro method for predicting the evolutionary response of HIV protease to a drug targeted thereagainst

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5436131A (en) * 1993-04-02 1995-07-25 Merck & Co., Inc. Color screening assay for identifying inhibitor resistant HIV protease mutants
US5952307A (en) * 1994-01-21 1999-09-14 Georgia Tech Research Corp. Basic α-aminoalkylphosphonate derivatives
US6063562A (en) * 1994-09-16 2000-05-16 Sepracor, Inc. In vitro method for predicting the evolutionary response of HIV protease to a drug targeted thereagainst
WO1998048008A1 (en) * 1997-04-23 1998-10-29 Plueckthun Andreas Methods for identifying nucleic acid molecules encoding (poly)peptides that interact with target molecules
WO1998054312A1 (en) * 1997-05-28 1998-12-03 Babraham Institute Ribosome complexes as selection particles for in vitro display and evolution of proteins
WO1999011777A1 (en) * 1997-09-03 1999-03-11 Biovation Limited Methods for protein screening

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
HANES JOZEF ET AL: "In vitro selection and evolution of functional proteins by using ribosome display", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 94, no. 10, 1997, pages 4937 - 4942, XP002077149, ISSN: 0027-8424 *
SCOTT CHRISTOPHER J ET AL: "Irreversible inhibition of the bacterial cysteine protease-transpeptidase sortase (SrtA) by substrate-derived affinity labels", BIOCHEMICAL JOURNAL, vol. 366, no. 3, 15 September 2002 (2002-09-15), pages 953 - 958, XP002318395, ISSN: 0264-6021 *

Also Published As

Publication number Publication date
WO2005007886A2 (en) 2005-01-27
GB0316314D0 (en) 2003-08-13

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