WO2005003377A3 - Method for monitoring reactions in real time - Google Patents

Method for monitoring reactions in real time Download PDF

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Publication number
WO2005003377A3
WO2005003377A3 PCT/CA2004/000966 CA2004000966W WO2005003377A3 WO 2005003377 A3 WO2005003377 A3 WO 2005003377A3 CA 2004000966 W CA2004000966 W CA 2004000966W WO 2005003377 A3 WO2005003377 A3 WO 2005003377A3
Authority
WO
WIPO (PCT)
Prior art keywords
real time
methods
monitoring reactions
substrate
product
Prior art date
Application number
PCT/CA2004/000966
Other languages
French (fr)
Other versions
WO2005003377A2 (en
Inventor
Yingfu Li
Razvan Nutiu
Original Assignee
Univ Mcmaster
Yingfu Li
Razvan Nutiu
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Mcmaster, Yingfu Li, Razvan Nutiu filed Critical Univ Mcmaster
Priority to EP04737905A priority Critical patent/EP1639124A2/en
Priority to CA002530237A priority patent/CA2530237A1/en
Priority to US10/562,677 priority patent/US20060275854A1/en
Publication of WO2005003377A2 publication Critical patent/WO2005003377A2/en
Publication of WO2005003377A3 publication Critical patent/WO2005003377A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

Methods for monitoring chemical reactions in real time are provided. The methods involve the use of a signaling aptamer that has a different affinity for the substrate and the product of the chemical reaction. The conversion from substrate to product is detected as a change in signal, in particular a change in fluorescent signal. The methods of the present invention are also useful to measure enzyme activity and to screen for enzyme inhibitors.
PCT/CA2004/000966 2003-07-01 2004-06-29 Method for monitoring reactions in real time WO2005003377A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP04737905A EP1639124A2 (en) 2003-07-01 2004-06-29 Method for monitoring reactions in real time
CA002530237A CA2530237A1 (en) 2003-07-01 2004-06-29 Method for monitoring reactions in real time
US10/562,677 US20060275854A1 (en) 2003-07-01 2004-06-29 Method for monitoring reactions in real time

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US48363603P 2003-07-01 2003-07-01
US60/483,636 2003-07-01
US55809904P 2004-04-01 2004-04-01
US60/558,099 2004-04-01

Publications (2)

Publication Number Publication Date
WO2005003377A2 WO2005003377A2 (en) 2005-01-13
WO2005003377A3 true WO2005003377A3 (en) 2005-04-14

Family

ID=33567696

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA2004/000966 WO2005003377A2 (en) 2003-07-01 2004-06-29 Method for monitoring reactions in real time

Country Status (3)

Country Link
EP (1) EP1639124A2 (en)
CA (1) CA2530237A1 (en)
WO (1) WO2005003377A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2900833B1 (en) 2012-09-26 2018-05-30 Promega Corporation Real-time monitoring

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
NUTIU R ET AL: "Structure-switching signaling aptamers", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, AMERICAN CHEMICAL SOCIETY, WASHINGTON, DC, US, vol. 125, no. 16, 23 April 2003 (2003-04-23), pages 4771 - 4778, XP002240695, ISSN: 0002-7863 *
NUTIU RAZVAN ET AL: "Signaling aptamers for monitoring enzymatic activity and for inhibitor screening.", CHEMBIOCHEM : A EUROPEAN JOURNAL OF CHEMICAL BIOLOGY. 6 AUG 2004, vol. 5, no. 8, 6 August 2004 (2004-08-06), pages 1139 - 1144, XP002309462, ISSN: 1439-4227 *
TYAGI S ET AL: "MOLECULAR BEACONS: PROBES THAT FLUORESCE UPON HYBRIDIZATION", NATURE BIOTECHNOLOGY, NATURE PUBLISHING, US, vol. 14, 1 March 1996 (1996-03-01), pages 303 - 308, XP000196024, ISSN: 1087-0156 *

Also Published As

Publication number Publication date
CA2530237A1 (en) 2005-01-13
EP1639124A2 (en) 2006-03-29
WO2005003377A2 (en) 2005-01-13

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