WO2005003377A3 - Method for monitoring reactions in real time - Google Patents

Method for monitoring reactions in real time Download PDF

Info

Publication number
WO2005003377A3
WO2005003377A3 PCT/CA2004/000966 CA2004000966W WO2005003377A3 WO 2005003377 A3 WO2005003377 A3 WO 2005003377A3 CA 2004000966 W CA2004000966 W CA 2004000966W WO 2005003377 A3 WO2005003377 A3 WO 2005003377A3
Authority
WO
WIPO (PCT)
Prior art keywords
real time
methods
monitoring reactions
substrate
product
Prior art date
Application number
PCT/CA2004/000966
Other languages
French (fr)
Other versions
WO2005003377A2 (en
Inventor
Yingfu Li
Razvan Nutiu
Original Assignee
Univ Mcmaster
Yingfu Li
Razvan Nutiu
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Mcmaster, Yingfu Li, Razvan Nutiu filed Critical Univ Mcmaster
Priority to CA002530237A priority Critical patent/CA2530237A1/en
Priority to EP04737905A priority patent/EP1639124A2/en
Priority to US10/562,677 priority patent/US20060275854A1/en
Publication of WO2005003377A2 publication Critical patent/WO2005003377A2/en
Publication of WO2005003377A3 publication Critical patent/WO2005003377A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

Methods for monitoring chemical reactions in real time are provided. The methods involve the use of a signaling aptamer that has a different affinity for the substrate and the product of the chemical reaction. The conversion from substrate to product is detected as a change in signal, in particular a change in fluorescent signal. The methods of the present invention are also useful to measure enzyme activity and to screen for enzyme inhibitors.
PCT/CA2004/000966 2003-07-01 2004-06-29 Method for monitoring reactions in real time WO2005003377A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CA002530237A CA2530237A1 (en) 2003-07-01 2004-06-29 Method for monitoring reactions in real time
EP04737905A EP1639124A2 (en) 2003-07-01 2004-06-29 Method for monitoring reactions in real time
US10/562,677 US20060275854A1 (en) 2003-07-01 2004-06-29 Method for monitoring reactions in real time

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US48363603P 2003-07-01 2003-07-01
US60/483,636 2003-07-01
US55809904P 2004-04-01 2004-04-01
US60/558,099 2004-04-01

Publications (2)

Publication Number Publication Date
WO2005003377A2 WO2005003377A2 (en) 2005-01-13
WO2005003377A3 true WO2005003377A3 (en) 2005-04-14

Family

ID=33567696

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA2004/000966 WO2005003377A2 (en) 2003-07-01 2004-06-29 Method for monitoring reactions in real time

Country Status (3)

Country Link
EP (1) EP1639124A2 (en)
CA (1) CA2530237A1 (en)
WO (1) WO2005003377A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2015530114A (en) 2012-09-26 2015-10-15 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ Real-time monitoring

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
NUTIU R ET AL: "Structure-switching signaling aptamers", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, AMERICAN CHEMICAL SOCIETY, WASHINGTON, DC, US, vol. 125, no. 16, 23 April 2003 (2003-04-23), pages 4771 - 4778, XP002240695, ISSN: 0002-7863 *
NUTIU RAZVAN ET AL: "Signaling aptamers for monitoring enzymatic activity and for inhibitor screening.", CHEMBIOCHEM : A EUROPEAN JOURNAL OF CHEMICAL BIOLOGY. 6 AUG 2004, vol. 5, no. 8, 6 August 2004 (2004-08-06), pages 1139 - 1144, XP002309462, ISSN: 1439-4227 *
TYAGI S ET AL: "MOLECULAR BEACONS: PROBES THAT FLUORESCE UPON HYBRIDIZATION", NATURE BIOTECHNOLOGY, NATURE PUBLISHING, US, vol. 14, 1 March 1996 (1996-03-01), pages 303 - 308, XP000196024, ISSN: 1087-0156 *

Also Published As

Publication number Publication date
WO2005003377A2 (en) 2005-01-13
EP1639124A2 (en) 2006-03-29
CA2530237A1 (en) 2005-01-13

Similar Documents

Publication Publication Date Title
TW200636075A (en) Detection of nucleic acid variation by cleavage-amplification method
EP2100899A3 (en) Compounds for proteasome enzyme inhibition
EP2312313A3 (en) Immobilized probes and methods of detecting conformationally altered prion proteins
GB2423819A (en) Apparatus and method for analysis of molecules
ATE513833T1 (en) THIENOPYRIDINE COMPOUNDS AND METHODS OF USE THEREOF
WO2003094838A3 (en) Simple catalytic dna biosensors for ions based on color changes
WO2005097809A3 (en) Synthesis of boronic ester and acid compounds
WO2005047474A3 (en) Nucleic acid detection method having increased sensitivity
EP1870713A4 (en) Biosample multiple autoanalyzer, method of autoanalysis and reaction cuvette
WO2005017646A3 (en) System, software and methods for biomarker identification
WO2007115205A3 (en) Improved methods, systems and compositions for monitoring enzyme activity and applications thereof
EP0976829A3 (en) Protein-deamidating enzyme, gene encoding the same, production process therefor, and use thereof
WO2001059149A3 (en) Peptide biosensors for anthrax protease
ATE512215T1 (en) METHOD AND MEANS FOR ENCOURAGEMENT, REMOVAL AND DETECTION OF LISTERIA
DK1187933T3 (en) Betalactam-containing compounds
WO2005012505A3 (en) Nanoparticle and microparticle based detection of cellular products
WO2003035896A3 (en) Chromogenic enzyme substrates and method for detecting beta-d-ribofuranosidase activity
EP1808493A3 (en) Substrates and methods for assaying deubiquitinating enzymes activity
NO20052544L (en) Product and method.
ATE503021T1 (en) METHOD FOR DETECTING ENDOGLYCOSIDASE ACTIVITY
WO2005031004A3 (en) Method for the specific rapid detection of beverage-spoiling micro-organisms
WO2007089542A3 (en) Systems and methods of analyzing nucleic acid polymers by using particle beams
WO2005003377A3 (en) Method for monitoring reactions in real time
WO2002044378A3 (en) Wnt signalling assay, methods and uses thereof
WO2004000995A3 (en) Methods, probes, and accessory molecules for detecting single nucleotide polymorphisms

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BW BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE EG ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NA NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): BW GH GM KE LS MW MZ NA SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
ENP Entry into the national phase

Ref document number: 2530237

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 2004737905

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2004737905

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2006275854

Country of ref document: US

Ref document number: 10562677

Country of ref document: US

WWP Wipo information: published in national office

Ref document number: 10562677

Country of ref document: US