WO2005001122A3 - Electrochemical detection of dna binding - Google Patents

Electrochemical detection of dna binding Download PDF

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Publication number
WO2005001122A3
WO2005001122A3 PCT/CA2004/000957 CA2004000957W WO2005001122A3 WO 2005001122 A3 WO2005001122 A3 WO 2005001122A3 CA 2004000957 W CA2004000957 W CA 2004000957W WO 2005001122 A3 WO2005001122 A3 WO 2005001122A3
Authority
WO
WIPO (PCT)
Prior art keywords
dna binding
electrochemical detection
nucleic acid
methods
impedence
Prior art date
Application number
PCT/CA2004/000957
Other languages
French (fr)
Other versions
WO2005001122A2 (en
Inventor
Chen-Zhong Li
Yi-Tao Long
Heinz-Bernhard Kraatz
Jeremy S Lee
Original Assignee
Adnavance Technologies Inc
Chen-Zhong Li
Yi-Tao Long
Heinz-Bernhard Kraatz
Jeremy S Lee
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Adnavance Technologies Inc, Chen-Zhong Li, Yi-Tao Long, Heinz-Bernhard Kraatz, Jeremy S Lee filed Critical Adnavance Technologies Inc
Publication of WO2005001122A2 publication Critical patent/WO2005001122A2/en
Publication of WO2005001122A3 publication Critical patent/WO2005001122A3/en
Priority to US11/317,671 priority Critical patent/US20060275786A1/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
    • G01N27/3277—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction being a redox reaction, e.g. detection by cyclic voltammetry

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nanotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • General Physics & Mathematics (AREA)
  • Composite Materials (AREA)
  • Materials Engineering (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Pathology (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

In one aspect, the invention provides methods and apparatus for detecting a protein binding to a nucleic acid by measuring the impedence of a nucleic acid layer on an electrode, for example by AC impedance spectroscopy. In one embodiment, such methods may for example be used to detect a mismatch in a nucleic acid duplex.
PCT/CA2004/000957 2003-03-05 2004-06-25 Electrochemical detection of dna binding WO2005001122A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US11/317,671 US20060275786A1 (en) 2003-03-05 2005-12-22 Electrochemical detection of DNA binding

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US48276603P 2003-06-27 2003-06-27
US60/482,766 2003-06-27

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US11/317,671 Continuation US20060275786A1 (en) 2003-03-05 2005-12-22 Electrochemical detection of DNA binding

Publications (2)

Publication Number Publication Date
WO2005001122A2 WO2005001122A2 (en) 2005-01-06
WO2005001122A3 true WO2005001122A3 (en) 2005-11-24

Family

ID=33552009

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA2004/000957 WO2005001122A2 (en) 2003-03-05 2004-06-25 Electrochemical detection of dna binding

Country Status (1)

Country Link
WO (1) WO2005001122A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080268444A1 (en) * 2006-04-17 2008-10-30 Fujifilm Corporation Detection method of SNPs
WO2008136957A1 (en) * 2007-04-30 2008-11-13 The University Of Pittsburgh - Of The Commonwealth System Of Higher Education Label-free, real-time detection system for molecular interaction analysis
JP2012513217A (en) 2008-12-22 2012-06-14 セルラ・インコーポレイテッド Method and genotyping panel for detecting alleles, genomes and transcriptomes
EP2440911A4 (en) * 2009-06-08 2013-02-13 Univ Western Ontario An electrochemical method and apparatus of identifying the presence of a target
GB201207585D0 (en) 2012-05-01 2012-06-13 Isis Innovation An electrode and use thereof

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999051778A1 (en) * 1998-04-08 1999-10-14 California Institute Of Technology Electrochemical sensor using intercalative, redox-active moieties
WO2001042508A2 (en) * 1999-12-09 2001-06-14 Motorola, Inc. Methods and compositions relating to electrical detection of nucleic acid reactions
US6306584B1 (en) * 1997-01-21 2001-10-23 President And Fellows Of Harvard College Electronic-property probing of biological molecules at surfaces
US20010034030A1 (en) * 1998-02-02 2001-10-25 John Hefti Method for detecting and classifying nucleic acid hybridization
WO2002012553A2 (en) * 2000-08-04 2002-02-14 Nanogen Recognomics Gmbh Method for detecting mutations in nucleotide sequences
WO2004079001A1 (en) * 2003-03-05 2004-09-16 University Of Saskatchewan Technologies Inc. Nucleic acid mismatch detection

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6306584B1 (en) * 1997-01-21 2001-10-23 President And Fellows Of Harvard College Electronic-property probing of biological molecules at surfaces
US20010034030A1 (en) * 1998-02-02 2001-10-25 John Hefti Method for detecting and classifying nucleic acid hybridization
WO1999051778A1 (en) * 1998-04-08 1999-10-14 California Institute Of Technology Electrochemical sensor using intercalative, redox-active moieties
WO2001042508A2 (en) * 1999-12-09 2001-06-14 Motorola, Inc. Methods and compositions relating to electrical detection of nucleic acid reactions
WO2002012553A2 (en) * 2000-08-04 2002-02-14 Nanogen Recognomics Gmbh Method for detecting mutations in nucleotide sequences
WO2004079001A1 (en) * 2003-03-05 2004-09-16 University Of Saskatchewan Technologies Inc. Nucleic acid mismatch detection

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BRETT C M A ET AL: "An EIS study of DNA-modified electrodes", ELECTROCHIMICA ACTA, ELSEVIER SCIENCE PUBLISHERS, BARKING, GB, vol. 44, no. 24, 31 July 1999 (1999-07-31), pages 4233 - 4239, XP004173501, ISSN: 0013-4686 *
LI C-Z ET AL: "Protein-DNA interaction: Impedance study of MutS binding to a DNA mismatch", CHEMICAL COMMUNICATIONS 07 MAR 2004 UNITED KINGDOM, vol. 10, no. 5, 27 January 2004 (2004-01-27), pages 574 - 575, XP002347377, ISSN: 1359-7345 *
LI, CHEN-ZHONG ET AL: "Electrochemical investigations of M-DNA self-assembled monolayers on gold electrodes", JOURNAL OF PHYSICAL CHEMISTRY B , 107(10), 2291 -2296 CODEN: JPCBFK; ISSN: 1520-6106, 19 February 2003 (2003-02-19), XP002347376 *
LONG Y-T ET AL: "AC impedance spectroscopy of native DNA and M-DNA", BIOPHYSICAL JOURNAL 01 MAY 2003 UNITED STATES, vol. 84, no. 5, 1 May 2003 (2003-05-01), pages 3218 - 3225, XP002347375, ISSN: 0006-3495 *
PATOLSKY F ET AL: "Electronic transduction of DNA sensing processes on surfaces: amplification of DNA detection and analysis of single-base mismatches by tagged liposomes", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, AMERICAN CHEMICAL SOCIETY, WASHINGTON, DC, US, vol. 123, no. 22, 6 June 2001 (2001-06-06), pages 5194 - 5205, XP002254092, ISSN: 0002-7863 *

Also Published As

Publication number Publication date
WO2005001122A2 (en) 2005-01-06

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