WO2004105685A3 - Ligation chimique en phase solide effectuee avec un agent de liaison mobile - Google Patents

Ligation chimique en phase solide effectuee avec un agent de liaison mobile Download PDF

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Publication number
WO2004105685A3
WO2004105685A3 PCT/US2004/015435 US2004015435W WO2004105685A3 WO 2004105685 A3 WO2004105685 A3 WO 2004105685A3 US 2004015435 W US2004015435 W US 2004015435W WO 2004105685 A3 WO2004105685 A3 WO 2004105685A3
Authority
WO
WIPO (PCT)
Prior art keywords
ligation
chemical ligation
solid phase
compositions
methods
Prior art date
Application number
PCT/US2004/015435
Other languages
English (en)
Other versions
WO2004105685A2 (fr
Inventor
Donald Low
Ying Hu
Paolo Botti
Original Assignee
Gryphon Therapeutics Inc
Donald Low
Ying Hu
Paolo Botti
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gryphon Therapeutics Inc, Donald Low, Ying Hu, Paolo Botti filed Critical Gryphon Therapeutics Inc
Priority to EP04752449A priority Critical patent/EP1631597A4/fr
Publication of WO2004105685A2 publication Critical patent/WO2004105685A2/fr
Publication of WO2004105685A3 publication Critical patent/WO2004105685A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
    • C07K1/042—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers characterised by the nature of the carrier
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/1072—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La présente invention concerne des procédés et des compositions destinés à la ligation chimique en phase solide compatible avec un milieu aqueux. Les procédés et les compositions selon l'invention comprennent la ligation chimique de premier et deuxième polymères, ledit premier polymère étant attaché à un support au moyen d'un agent de liaison mobile. L'agent de liaison mobile peut être clivé ou bien déplacé dans des conditions aqueuses compatibles avec le retrait subséquent du produit de ligation dudit support solide. Les procédés et les compositions selon l'invention sont particulièrement utiles pour la ligation de peptides et de polypeptides sur un support solide. Le système de ligation selon l'invention peut être utilisé pour une grande diversité de molécules et peut par conséquent servir à la génération de polymères liés tels que des peptides, des polypeptides et d'autres polymères contenant des acides aminés.
PCT/US2004/015435 2003-05-22 2004-05-17 Ligation chimique en phase solide effectuee avec un agent de liaison mobile WO2004105685A2 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP04752449A EP1631597A4 (fr) 2003-05-22 2004-05-17 Ligation chimique en phase solide effectuee avec un agent de liaison mobile

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US47329403P 2003-05-22 2003-05-22
US60/473,294 2003-05-22

Publications (2)

Publication Number Publication Date
WO2004105685A2 WO2004105685A2 (fr) 2004-12-09
WO2004105685A3 true WO2004105685A3 (fr) 2005-06-09

Family

ID=33490583

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/015435 WO2004105685A2 (fr) 2003-05-22 2004-05-17 Ligation chimique en phase solide effectuee avec un agent de liaison mobile

Country Status (2)

Country Link
EP (1) EP1631597A4 (fr)
WO (1) WO2004105685A2 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040248162A1 (en) * 2002-12-17 2004-12-09 Affymetrix, Inc. Releasable polymer arrays
US7547775B2 (en) 2004-12-31 2009-06-16 Affymetrix, Inc. Parallel preparation of high fidelity probes in an array format
GB0505200D0 (en) * 2005-03-14 2005-04-20 Univ Southampton Process
EP1871403B1 (fr) 2005-03-31 2010-09-08 Amylin Pharmaceuticals, Inc. Compositions et procedes pour le controle, la prevention et le traitement des troubles de l'alimentation
ES2389747T3 (es) 2007-02-05 2012-10-31 Amylin Pharmaceuticals, Inc. Péptidos FN-38 para su uso en el tratamiento de desórdenes psicóticos y de ansiedad
US8409526B2 (en) * 2010-12-09 2013-04-02 General Electric Company Cellulose substrates, compositions and methods for storage and analysis of biological materials
MX365465B (es) 2013-03-21 2019-06-04 Sanofi Aventis Deutschland Sintesis de productos peptidicos que contienen imida ciclica.
HUE034308T2 (en) 2013-03-21 2018-02-28 Sanofi Aventis Deutschland Preparation of hydantoin-containing peptide products
KR20160062035A (ko) 2013-09-11 2016-06-01 덴마크스 텍니스케 유니버시테트 히드라지드 및 피라노피라졸의 고체상 합성용 광분해성 링커
KR20210072021A (ko) 2018-10-01 2021-06-16 유니베르시떼 드 제네브 생물학 분석에 적합한 복수의 폴리펩티드 변이체의 생산 방법

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5141966A (en) * 1989-12-19 1992-08-25 Exploaterings Ab T.B.F. Metalchelating, hydrophilic polymer
US6271345B1 (en) * 1996-07-03 2001-08-07 Basf Aktiengesellschaft Enzyme cleavable linker bound to solid phase for organic compound synthesis
US6326468B1 (en) * 1997-06-13 2001-12-04 Gryphon Sciences Solid phase native chemical ligation of unprotected or n-terminal cysteine protected peptides in aqueous solution

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1616021A4 (fr) * 2002-12-30 2006-08-30 Gryphon Therapeutics Inc Ligation de polymeres multiplex

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5141966A (en) * 1989-12-19 1992-08-25 Exploaterings Ab T.B.F. Metalchelating, hydrophilic polymer
US6271345B1 (en) * 1996-07-03 2001-08-07 Basf Aktiengesellschaft Enzyme cleavable linker bound to solid phase for organic compound synthesis
US6326468B1 (en) * 1997-06-13 2001-12-04 Gryphon Sciences Solid phase native chemical ligation of unprotected or n-terminal cysteine protected peptides in aqueous solution

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
BRIK A. ET AL: "Protein Synthesis by Solid-Phase Chemical Ligation Using a Safety Catch Linker", J. ORG. CHEM., vol. 65, 2000, pages 3829 - 3835, XP002987909 *
CANNE L.E. ET AL: "Chemical Protein Synthesis by Solid Phase Ligation of Unprotected Peptide Segments", J. AM. CHEM. SOC., vol. 121, 1999, pages 8720 - 8727, XP002987910 *
ENGLEBRETSEN D.R. ET AL: "A Novel Thioether Linker: Chemical Synthesis of a HIV-1 Protease Analogue by Thioether Ligation", TETRAHEDRON LETTERS, vol. 36, no. 48, 1995, pages 8871 - 8874, XP004026856 *
GAERTNER H.F. ET AL: "Site-Specific Religation of G-CSF Fragments through a Thioether Bond", BIOCONJ. CHEM., vol. 5, 1994, pages 333 - 338, XP002987911 *
ROSE K. ET AL: "Facile Synthesis of Homogeneous Artificial Proteins", J. AM. CHEM. SOC., vol. 116, 1994, pages 30 - 33, XP002301786 *
ROUTLEDGE A.R. ET AL: "The Use of a Dithiane Protected Benzoin Photolabile Safety Catch Linker for Solid-Phase Synthesis", TETRA. LETTERS, vol. 38, no. 7, 1997, pages 1227 - 1230, XP004034241 *
SCHNOLZER M. ET AL: "Constructing Proteins by Dovetailing Unprotected Synthetic Peptides: Backbone-Engineered HIV Protease", SCIENCE, vol. 256, no. 5054, 10 April 1992 (1992-04-10), pages 221 - 225, XP002078246 *
See also references of EP1631597A4 *
SEITZ O. ET AL: "HYCRON, an Allylic Anchor for High-Efficiency Solid Phase Synthesis of Protected Peptides and Glycopeptides", J. ORG. CHEM., vol. 62, 1997, pages 813 - 826, XP002162441 *
ZHANG L.Z. ET AL: "Preparation of functionally active cell-permeable peptides by single-step ligation of two peptide modules", PNAS, vol. 95, August 1998 (1998-08-01), pages 9184 - 9189, XP002133028 *

Also Published As

Publication number Publication date
EP1631597A4 (fr) 2007-07-11
WO2004105685A2 (fr) 2004-12-09
EP1631597A2 (fr) 2006-03-08

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