WO2004101809A2 - Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture - Google Patents
Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture Download PDFInfo
- Publication number
- WO2004101809A2 WO2004101809A2 PCT/US2004/015196 US2004015196W WO2004101809A2 WO 2004101809 A2 WO2004101809 A2 WO 2004101809A2 US 2004015196 W US2004015196 W US 2004015196W WO 2004101809 A2 WO2004101809 A2 WO 2004101809A2
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- anion exchange
- exchange resin
- solution
- nucleic acids
- resin
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Definitions
- the present invention relates generally to the field of analysis of nucleic acids by mass spectrometry and provides methods and kits useful for this purpose.
- Eiectrospray ionization mass spectrometry has become an important technique for the analysis of biopolymers.
- the multiple charging phenomenon allows fast, accurate and precise molecular mass measurement, identification of modifications and more detailed structural studies for very high-mass biopolymers.
- PCR products are "sized” using traditional biochemical techniques such as standard gel electrophoresis using either intercalating dyes or fluorescently labeled primers.
- the TaqmanTM assay which is widely used in a number of PCR- based diagnostic kits, confirms the presence (or absence) of a specific PCR product but provides no direct readout on the size of the amplicon.
- So-called "real-time" PCR devices which measure the laser-induced fluorescence of the PCR product during the amplification cycles, are used to quantify the amplification of DNA from a given DNA template and primer set. These methods have limited utility for relatively small amplicons (less than 150 base pairs), owing to the proportionately high fluorescence background, and do not provide any information with respect to amplicon heterogeneity or exact length.
- mass spectrometry has several potential advantages as a platform on which to characterize PCR products, including speed, sensitivity, and mass accuracy. Because the exact mass of each of the bases which comprise DNA is known with great accuracy, a high-precision mass measurement obtained via mass spectrometry can be used to derive a base composition within the experimentally obtained mass measurement uncertainty (J. Aaserud, Z. Guan, D.P. Little and F.W. McLafferty, Int. J. Mass Spectrom. Ion Processes, 1997, 167/168, 705-712. and D.C. Muddiman, G.A. Anderson, S.A. Hofstadler and R.D. Smith, Anal. Chem.
- MALDI matrix assisted, laser desorption ionization
- ESI eiectrospray
- the charges are statistically distributed amongst the analyte's available charge sites, leading to the possible formation of multiply charged ions conditions.
- ESI requires relatively clean samples and is notoriously intolerable of cationic salts, detergents, and many buffering agents commonly used in biochemical laboratories.
- the buffer system commonly employed in the polymerase chain reaction includes eiectrospray incompatible reagents such as 50 mM KC1, 2 mM MgCl 2 , 10 mM Tris-HCl, and each of the four deoxynucleotide triphosphates (dNTPs) at 200 :M.
- dNTPs deoxynucleotide triphosphates
- Even the presence of relatively low concentrations of metal cations (less than 100 :M) can significantly reduce MS sensitivity for oligonucleotides as the signal for each molecular ion is spread out over multiple salt adducts.
- effective ESI-MS of PCR products requires that the salt concentration be reduced by more than a factor of 1000 prior to analysis.
- Ethanol precipitation has been used to desalt PCR products for subsequent MS analysis as short oligonucleotides and salts are removed while the sample is concentrated (M.T. Krahmer, Y.A. Johnson, J.J. Walters, K.F. Fox, A. Fox and M. Nagpal, Eiectrospray Anal. Chem. 1999, 77, 2893-2900; T. Tsuneyoshi, K. Ishikawa, Y. Koga, Y. Naito, S. Baba, H. Terunuma, R. Arakawa and D.J. Prockop Rapid Commun. Mass Spectrom. 1997, 11, 719-722; and D.C. Muddiman, D.S.
- the PCR product can be precipitated from concentrated ammonium acetate solutions, either overnight at 5 °C or over the course of 10-15 min with cold (-20 °C) ethanol.
- a precipitation step alone is generally insufficient to obtain PCR products which are adequately desalted to obtain high-quality ESI spectra; consequently, precipitation is generally followed by a dialysis step to further desalt the sample (D.C. Muddiman, D.S. Wunschel, C.L. Liu, L.
- DNA purification kits may also be used in conjunction with traditional desalting techniques such as microdialysis (S. Hahner, A. Schneider, A. Ingendoh and J. Mosner Nucleic Acids Res. 2000, 28, e82/i-e82/viii; and A.P. Null, L.T. George and D.C. Muddiman J. Am. Soc. Mass Spectrom. 2002, 13, 338-344).
- Other purification techniques such as gel electrophoresis followed by high-performance liquid chromatography or drop dialysis, or cation exchange using membranes or resins have also been used to obtain high-purity, desalted DNA for MS detection (L.M. Benson, S.-S. Juliane, P.D.
- solution capture for purification of PCR products for analysis by mass spectrometry has substantially reduced the cost associated with sample preparation by eliminating the need to pack, equilibrate, and test a column.
- the retail cost of the current procedure using a pipette tip packed with anion exchange resin exemplified by ZipTipTM AX (Millipore, Bedford, MA) is approximately $1.77 per pipette tip (for each sample).
- the estimated cost of solution capture of PCR products is $0.10 per sample and takes into account the combination of anion exchange resin and filter plate.
- the time required for solution capture purification of PCR products is approximately 10 minutes per 96 well plate in contrast to the previous method which employs the ZipTipTM AX pipette tips and requires approximately 20 minutes.
- the present invention is directed to, inter alia, solution capture methods of purifying a solution comprising one or more nucleic acids for subsequent analysis by eiectrospray mass spectrometry, or any other analysis, by adding an anion exchange resin to the solution and mixing to yield a suspension of the anion exchange resin in the solution wherein the nucleic acid binds to the anion exchange resin, isolating the anion exchange resin from the solution, washing the anion exchange resin to remove one or more contaminants with one or more wash buffers while retaining bound nucleic acid, eluting the nucleic acid, from the ion exchange resin with an elution buffer, and optionally, analyzing the nucleic acids by eiectrospray mass spectrometry.
- the anion exchange resin may have a strong anion exchange functional group such as a quaternary amine or a weak anion exchange functional group such as, for example, polyethyleneimine, charged aromatic amine, diethylaminomethyl, or diethylaminoethyl.
- Such weak anion exchange resins comprise functional groups with pK a values of 9.0 or greater.
- the present invention is further directed to kits for purification of nucleic acids comprising a filter plate comprising a plurality of wells or a tube rack comprising a plurality of tubes, an anion exchange resin, at least one anion exchange wash buffer and an ESI-MS- compatible elution buffer.
- Figure 1 is a process diagram outlining the steps of the present invention beginning with the addition and mixing of anion exchange resin into the sample of nucleic acids (100).
- the resin is then isolated from the solution (110) and washed with an appropriate wash buffer to remove contaminants from the resin (120) after which, the nucleic acids are eluted from the resin by an eiectrospray ionization (ESI)-compatible elution buffer, which makes possible the final step of analysis of the nucleic acids by ESI-mass spectrometry (140).
- ESI eiectrospray ionization
- Figure 2 is a comparison of ESI-MS spectra for purified PCR products obtained by purification with ZipTipsTM (top panel) and by the solution capture purification method of the present invention. The comparison indicates that purification by the solution capture method is equally effective as the previously validated method which employs ZipTipsTM.
- One embodiment of the method of solution capture purification of nucleic acids for analysis by mass spectrometry is outlined in Figure 1.
- the methods described herein can be used for other types of analysis, in addition to mass spectrometry as known to those skilled in the art.
- the methods comprise the following steps: Addition and mixing of an anion exchange resin into a solution of nucleic acids (100), isolating the anion exchange resin from the solution (110), washing the anion exchange resin to remove contaminants (120), eluting the nucleic acids, (free of contaminants) from the anion exchange resin (130), and, optionally, analyzing the nucleic acid by ESI mass spectrometry.
- a strong cation exchange functional group such as a quaternary amine for example, is employed as the functional group of the anion exchange resin. Additional strong anion exchange functional groups are known to those skilled in the art.
- a weak anion exchange functional group is a suitable anion exchange functional group, such as polyethyleneimine, charged aromatic amine, diethylaminomethyl, or diethylaminoethyl, for example, are employed as the functional group of the anion exchange resin. Such functional groups have pK a values of 9.0 or greater.
- Commercial products of weak anion exchange resin include, but are not limited to; Baker PEI, Baker DEAM, Dionex ProPacTM WAX, Millipore PEI, Applied Biosystems PorosTM PI.
- the mixing of the anion exchange resin into the solution of nucleic acids is effected by repeated pipetting, vortexing, sonication, shaking, or any other method that results in suspension of the anion exchange resin in the solution containing the nucleic acids.
- dry anion exchange resin is added directly to the solution of nucleic acids or contained within a microtube or the well of a micro filter plate into which the solution of nucleic acids is added prior to mixing.
- the anion exchange resin is pre-hydrated and added directly to the solution of nucleic acids or contained within a microtube or a well of a microfilter plate into which the solution of nucleic acids is added prior to mixing.
- the anion exchange resin which contains bound nucleic acids is isolated from the solution by filtration.
- Filtration can be effected, for example, using a filter plate in a 96- or 384- well format which enables high-throughput purification of multiple samples, or in any other container or plurality of containers equipped with a filter. Other well format plates can also be used.
- Membranes useful for filtration include but are not limited to those composed of the following materials: polytetrafluoroethylene (PTFE), polyvinyldifluoro (PVDF), polypropylene, polyethylene, glass fiber, polycarbonate and polysulfone. Filtering may by accomplished by vacuum, centrifugation, or positive pressure displacement with fluids or gases, or any other method that effects the isolation of the anion exchange resin from the solution. Methods of filtering are well known to those skilled in the art.
- the anion exchange resin comprises an anion exchange functional group which is linked to magnetic beads.
- a magnetic field can be activated to compress the magnetic bead resin so that liquid can be aspirated off by the liquid handler.
- the anion exchange resin which contains bound nucleic acids is washed to remove one or more contaminants.
- Contaminants include, but are not limited to: proteins such as reverse transcriptase and restriction enzymes, polymers, salts, buffer additives, or any of the various components of an amplification reaction such as polymerases nucleotide triphosphates or any combination thereof.
- proteins such as reverse transcriptase and restriction enzymes, polymers, salts, buffer additives, or any of the various components of an amplification reaction such as polymerases nucleotide triphosphates or any combination thereof.
- more than one wash buffer may be useful for removal of contaminants.
- Washing of the anion exchange resin can be effected with aqueous solutions of ammonium acetate in the millimolar range from about 20 mM to about 500 mM NH 4 OAc or with about 20 mM to about 500 mM NH 4 HCO 3 . Washing with about 10% to about 50% methanol, about 20% to about 50% methanol, or about 10% to about 30% methanol is useful as a final wash step. Methanol can be replaced by other suitable alcohols known to those skilled in the art.
- elution of nucleic acids from the anion exchange resin is accomplished using an ESI-compatible solution at alkaline pH of about pH 9 or greater such as an aqueous solution of about 2% to about 8% ammonium hydroxide or an aqueous solution of about 10 mM to about 50 mM, or 25mM piperidine, about 10 mM to about 50 mM, or 25mM imidazole and about 30% methanol or other suitable alcohol.
- an ESI- compatible solution is a solution which does not have a detrimental effect on the function of an eiectrospray (ESI) source.
- the term "about” means ⁇ 10% of the term being modified.
- “about” 10 mM means 9 to 11 mM.
- the present invention also provides kits for purification of nucleic acids by the solution capture method of the present invention.
- the kit may comprise a sufficient quantity of anion exchange resin.
- the anion exchange resin is a weak anion exchange resin such as one of the following commercially available weak anion exchange resins: Baker polyethyleneimine resin, Baker diethylaminomethyl resin, Dionex ProPacTM WAX, Millipore polyethyleneimine, and Applied Biosystems POROSTM PI.
- the kit may comprise a filter plate such as a 96- or 384-well filter plate or a microtube rack comprising a plurality of micro filter tubes.
- dry anion exchange resin is pre-loaded into the wells of a filter plate or microtube rack and can be either pre-hydrated or in the dry (powder) form.
- the kit may also comprise a filter plate comprising a plurality of wells or a tube rack comprising a plurality of tubes, an anion exchange resin, at least one anion exchange wash buffer and an ESI-MS-compatible elution buffer.
- the kit may comprise a 96 or 384 well plate containing either pre- hydrated anion exchange resin or dry anion exchange resin, a second 96 or 384 well sample mixing plate, a 96 or 384 well filter plate, a resin treatment buffer, one or more wash buffers, and an ESI-compatible elution buffer.
- the nucleic acid solution is a PCR product prepared for identification of an unknown bioagent and contained in an individual well of a 96 well sample plate on the deck of an automated liquid handler.
- the liquid handler is the cornerstone for many laboratory processes associated with drug discovery and high throughput screening.
- the dispensing and aspiration functions of liquid handlers are used to perform solvent/reagent additions, dilutions, plate replications consolidation, redistribution and other microplate-based tasks and typically use disposable pipette tips for transferring liquids. Programming of liquid handlers to perform the various liquid handling tasks of this embodiment is well within the capabilities of one with ordinary skill in the art without undue experimentation.
- the liquid handler is programmed to transfer and mix a predetermined volume of a suspension of anion exchange resin into the well containing the PCR product.
- the resin suspension can be contained in a resin source container such a 96 well plate and transferred to the PCR product plate by the liquid handler. Mixing is performed by the liquid handler via repeated dispensation and aspiration of the PCR-resin mixture and binding of nucleic acids to the resin occurs at this stage.
- the liquid handler transfers the PCR product-resin mixture from the 96 well plate to a 96 or 384 well filter plate.
- the filter plate can be removed from the liquid handler deck and the resin can be isolated from the solution by centrifugation or positive pressure displacement before returning the filter plate to the liquid handler deck.
- the resin containing bound nucleic acids is then washed one or more times with an appropriate wash solution such as about 100 mM NH 4 HCO 3 with the liquid handler pipetting the wash solution into the filter plate, followed by centrifugation, vacuum, or positive pressure displacement followed by one or more washes with about 20% to about 50% methanol before returning the filter plate containing the resin and bound nucleic acids to the liquid handler deck.
- an ESI compatible elution buffer such as an aqueous solution of about 25mM piperidine, about 25mM imidazole and about 50% methanol.
- This ESI compatible buffer may also optionally contain an internal standard used to calibrate the ESI mass spectrometer during the subsequent ESI mass spectrometry analysis.
- an internal standard used to calibrate the ESI mass spectrometer during the subsequent ESI mass spectrometry analysis.
- nucleic acid is isolated from the organisms and amplified by PCR using standard methods prior to BCS determination by mass spectrometry.
- Nucleic acid is isolated, for example, by detergent lysis of bacterial cells, centrifugation and ethanol precipitation. Nucleic acid isolation methods are described in, for example, Current Protocols in Molecular Biology (Ausubel et al.) and Molecular Cloning; A Laboratory Manual (Sambrook et al).
- the nucleic acid is then amplified using standard methodology, such as PCR, with primers which bind to conserved regions of the nucleic acid which contain an intervening variable sequence as described below.
- General Genomic DNA Sample Prep Protocol Raw samples are filtered using Supor-
- Swab Sample Protocol Allegiance S/P brand culture swabs and collection/transport system are used to collect samples. After drying, swabs are placed in 17x100 mm culture tubes (VWR International) and the genomic nucleic acid isolation is carried out automatically with a Qiagen Mdx robot and the Qiagen QIAamp DNA Blood BioRobot Mdx genomic preparation kit (Qiagen, Valencia, CA).
- the mass spectrometer used is a Bruker Daltonics (Billerica, MA) Apex II 70e eiectrospray ionization Fourier transform ion cyclotron resonance mass spectrometer (ESI-
- FTICR-MS FTICR-MS that employs an actively shielded 7 Tesla superconducting magnet. All aspects of pulse sequence control and data acquisition were performed on a 1.1 GHz Pentium II data station running Bruker' s Xmass software. 20 ⁇ L sample aliquots were extracted directly from 96-well microtiter plates using a CTC HTS PAL autosampler (LEAP Technologies, Carrboro, NC) triggered by the data station. Samples were injected directly into the ESI source at a flow rate of 75 ⁇ L/hr.
- Ions were formed via eiectrospray ionization in a modified Analytica (Branford, CT) source employing an off axis, grounded eiectrospray probe positioned ca. 1.5 cm from the metalized terminus of a glass desolvation capillary. The atmospheric pressure end of the glass capillary is biased at 6000 V relative to the ESI needle during data acquisition. A counter-current flow of dry N 2 /O 2 was employed to assist in the desolvation process. Ions were accumulated in an external ion reservoir comprised of an rf-only hexapole, a skimmer cone, and an auxiliary gate electrode, prior to injection into the trapped ion cell where they were mass analyzed.
- Spectral acquisition was performed in the continuous duty cycle mode whereby ions were accumulated in the hexapole ion reservoir simultaneously with ion detection in the trapped ion cell. Following a 1.2 ms transfer event, in which ions were transferred to the trapped ion cell, the ions were subjected to a 1.6 ms chirp excitation corresponding to 8000 - 500 m/z. Data was acquired over an m/z range of 500 - 5000 (1M data points over a 225K Hz bandwidth). Each spectrum was the result of co-adding 32 transients. Transients were zero-filled once prior to the magnitude mode Fourier transform and post calibration using the internal mass standard. The
- ICR-2LS software package G. A. Anderson, J. E. Bruce (Pacific Northwest National Laboratory, Richland, WA, 1995) was used to deconvolute the mass spectra and calculate the mass of the monoisotopic species using an "averaging" fitting routine (M. W. Senko, S. C. Beu, F. W. McLafferty, J. Am. Soc. Mass Spectrom. 1995, 6, 229) modified for DNA. Using this approach, monoisotopic molecular weights were calculated.
- Example 3 Procedure for Semi-automated Purification of a PCR mixture using Commercially Available ZipTipsTM
- ZipTipsTM AX (Millipore Corp. Bedford, MA)
- the following steps were programmed to be performed by an EvolutionTM P3 liquid handler (Perkin Elmer) with fluids being drawn from stock solutions in individual wells of a 96-well plate (Marshall Bioscience): loading of a rack of ZipTipsTM AX; washing of ZipTipsTM AX with 15 :1 of 10% NFL OH/50% methanol; washing of ZipTipsTM AX with 15 :1 of water 8 times; washing of ZipTipsTM AX with 15 :1 of 100 mM NH 4 OAc.
- EvolutionTM P3 liquid handler Perkin Elmer
- Corning 384-well glass fiber filter plates were pre-treated with two rinses of 250 :1 NH 4 OH and two rinses of 100 :1 NH 4 HCO 3 .
- the following steps were programmed to be performed by the EvolutionTM P3 liquid handler: addition of 0.05 to 10 :1 of pre-treated ProPacTM WAX weak anion exchange resin (30 :1 of a 1 :60 dilution) to a 50 :1 PCR reaction mixture (80 :1 total volume) in a 96-well plate; mixing of the solution by aspiration/dispensation for 2.5 minutes; and transfer of the solution to a pre-treated Corning 384- well glass fiber filter plate. This step was followed by centrifugation to remove liquid from the resin and is performed manually, or under the control of a robotic arm.
- the resin containing nucleic acids was then washed by rinsing three times with 200 :1 of 100 mM NH 4 OAc, 200 :1 of 40 mM NH 4 HCO 3 with removal of buffer by centrifugation for about 15 seconds followed by rinsing three times with 20% methanol for about 15 seconds. The final rinse was followed by an extended centrifugation step (1-2 minutes).
- Elution of the nucleic acids from the resin was accomplished by addition of 40 :1 elution/electrospray buffer (25 mM piperidine/25 mM imidazole/35% methanol and 50 nM of an internal standard oligonucleotide for calibration of mass spectrometry signals) followed by elution from the 384-well filter plate into a 384-well catch plate by centrifugation.
- the eluted nucleic acids in this condition were amenable to analysis by ESI-MS (See Figure 2).
- the time required for purification of samples in a single 96-well plate using a liquid handler is approximately five minutes.
- Bacillus anthracis DNA was isolated and amplified by PCR using a primer pair that amplifies a section of the lef gene of 5. anthracis ranging from residues 756-872.
- Shown in Figure 2 is a comparison of ESI-MS spectra for purified PCR products obtained by purification with ZipTipsTM (top panel) and by the solution capture purification method of the present invention. The comparison indicates that purification by the solution capture method is equally effective as the previously validated method which employs ZipTipsTM. However, purification by solution capture represents a significant cost savings and is more efficient.
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- Crystallography & Structural Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plant Pathology (AREA)
- Immunology (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Sampling And Sample Adjustment (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Claims
Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2004800161879A CN1806050B (en) | 2003-05-13 | 2004-05-13 | Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture |
CA2525498A CA2525498C (en) | 2003-05-13 | 2004-05-13 | Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture |
JP2006533082A JP2007516425A (en) | 2003-05-13 | 2004-05-13 | Rapid purification of nucleic acids by solution capture for later analysis using mass spectrometry |
AU2004239340A AU2004239340A1 (en) | 2003-05-13 | 2004-05-13 | Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture |
EP04752257A EP1623039B1 (en) | 2003-05-13 | 2004-05-13 | Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture |
AU2008205432A AU2008205432B8 (en) | 2003-05-13 | 2008-08-14 | Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US47054703P | 2003-05-13 | 2003-05-13 | |
US60/470,547 | 2003-05-13 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2004101809A2 true WO2004101809A2 (en) | 2004-11-25 |
WO2004101809A3 WO2004101809A3 (en) | 2005-09-01 |
Family
ID=33452406
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2004/015196 WO2004101809A2 (en) | 2003-05-13 | 2004-05-13 | Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture |
Country Status (8)
Country | Link |
---|---|
US (1) | US7964343B2 (en) |
EP (1) | EP1623039B1 (en) |
JP (2) | JP2007516425A (en) |
KR (1) | KR100779138B1 (en) |
CN (1) | CN1806050B (en) |
AU (2) | AU2004239340A1 (en) |
CA (1) | CA2525498C (en) |
WO (1) | WO2004101809A2 (en) |
Cited By (46)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2006034294A1 (en) * | 2004-09-17 | 2006-03-30 | Isis Pharmaceuticals, Inc. | Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture |
WO2007066227A2 (en) * | 2005-12-05 | 2007-06-14 | Novartis Vaccines And Diagnostics Srl | Liquid chromatography-mass spectrometry analysis of samples using an ionic eluent comprising a volatile ionic salt |
GB2445442A (en) * | 2006-09-26 | 2008-07-09 | Ge Healthcare Bio Sciences | Nucleic acid purification using anion exchange |
JP2008538505A (en) * | 2005-04-21 | 2008-10-30 | アイシス ファーマシューティカルズ インコーポレイティッド | Composition for use in bacterial identification |
JP2008547031A (en) * | 2005-06-30 | 2008-12-25 | バイオクラテス ライフ サイエンシズ アクチェンゲゼルシャフト | Equipment for quantitative analysis of metabolite characteristics |
US7655794B2 (en) | 2006-09-26 | 2010-02-02 | Ge Healthcare Bio-Sciences Corp. | Nucleic acid purification method |
US7655793B2 (en) | 2006-09-26 | 2010-02-02 | Ge Healthcare Bio-Sciences Corp. | Nucleic acid purification method |
WO2011047307A1 (en) | 2009-10-15 | 2011-04-21 | Ibis Biosciences, Inc. | Multiple displacement amplification |
US7956175B2 (en) | 2003-09-11 | 2011-06-07 | Ibis Biosciences, Inc. | Compositions for use in identification of bacteria |
US7964343B2 (en) | 2003-05-13 | 2011-06-21 | Ibis Biosciences, Inc. | Method for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture |
US8017358B2 (en) | 2001-03-02 | 2011-09-13 | Ibis Biosciences, Inc. | Method for rapid detection and identification of bioagents |
WO2011112718A1 (en) | 2010-03-10 | 2011-09-15 | Ibis Biosciences, Inc. | Production of single-stranded circular nucleic acid |
US8026084B2 (en) | 2005-07-21 | 2011-09-27 | Ibis Biosciences, Inc. | Methods for rapid identification and quantitation of nucleic acid variants |
US8057993B2 (en) | 2003-04-26 | 2011-11-15 | Ibis Biosciences, Inc. | Methods for identification of coronaviruses |
US8073627B2 (en) | 2001-06-26 | 2011-12-06 | Ibis Biosciences, Inc. | System for indentification of pathogens |
US8084207B2 (en) | 2005-03-03 | 2011-12-27 | Ibis Bioscience, Inc. | Compositions for use in identification of papillomavirus |
US8097416B2 (en) | 2003-09-11 | 2012-01-17 | Ibis Biosciences, Inc. | Methods for identification of sepsis-causing bacteria |
US8148163B2 (en) | 2008-09-16 | 2012-04-03 | Ibis Biosciences, Inc. | Sample processing units, systems, and related methods |
US8158936B2 (en) | 2009-02-12 | 2012-04-17 | Ibis Biosciences, Inc. | Ionization probe assemblies |
US8163895B2 (en) | 2003-12-05 | 2012-04-24 | Ibis Biosciences, Inc. | Compositions for use in identification of orthopoxviruses |
US8173957B2 (en) | 2004-05-24 | 2012-05-08 | Ibis Biosciences, Inc. | Mass spectrometry with selective ion filtration by digital thresholding |
US8182992B2 (en) | 2005-03-03 | 2012-05-22 | Ibis Biosciences, Inc. | Compositions for use in identification of adventitious viruses |
US8187814B2 (en) | 2004-02-18 | 2012-05-29 | Ibis Biosciences, Inc. | Methods for concurrent identification and quantification of an unknown bioagent |
US8214154B2 (en) | 2001-03-02 | 2012-07-03 | Ibis Biosciences, Inc. | Systems for rapid identification of pathogens in humans and animals |
US8268565B2 (en) | 2001-03-02 | 2012-09-18 | Ibis Biosciences, Inc. | Methods for identifying bioagents |
US8298760B2 (en) | 2001-06-26 | 2012-10-30 | Ibis Bioscience, Inc. | Secondary structure defining database and methods for determining identity and geographic origin of an unknown bioagent thereby |
WO2013036603A1 (en) | 2011-09-06 | 2013-03-14 | Ibis Biosciences, Inc. | Sample preparation methods |
US8407010B2 (en) | 2004-05-25 | 2013-03-26 | Ibis Biosciences, Inc. | Methods for rapid forensic analysis of mitochondrial DNA |
US8534447B2 (en) | 2008-09-16 | 2013-09-17 | Ibis Biosciences, Inc. | Microplate handling systems and related computer program products and methods |
US8546082B2 (en) | 2003-09-11 | 2013-10-01 | Ibis Biosciences, Inc. | Methods for identification of sepsis-causing bacteria |
US8550694B2 (en) | 2008-09-16 | 2013-10-08 | Ibis Biosciences, Inc. | Mixing cartridges, mixing stations, and related kits, systems, and methods |
EP2647709A1 (en) * | 2012-04-05 | 2013-10-09 | F. Hoffmann-La Roche AG | Amine compounds for the selective preparation of biological samples |
US8563250B2 (en) | 2001-03-02 | 2013-10-22 | Ibis Biosciences, Inc. | Methods for identifying bioagents |
WO2014032990A1 (en) * | 2012-08-30 | 2014-03-06 | Qiagen Gmbh | Method of determining the presence or absence of a target nucleic acid in a cell sample |
WO2014052590A1 (en) | 2012-09-26 | 2014-04-03 | Ibis Biosciences, Inc. | Swab interface for a microfluidic device |
US8822156B2 (en) | 2002-12-06 | 2014-09-02 | Ibis Biosciences, Inc. | Methods for rapid identification of pathogens in humans and animals |
US8871471B2 (en) | 2007-02-23 | 2014-10-28 | Ibis Biosciences, Inc. | Methods for rapid forensic DNA analysis |
US8950604B2 (en) | 2009-07-17 | 2015-02-10 | Ibis Biosciences, Inc. | Lift and mount apparatus |
US9068017B2 (en) | 2010-04-08 | 2015-06-30 | Ibis Biosciences, Inc. | Compositions and methods for inhibiting terminal transferase activity |
US9149473B2 (en) | 2006-09-14 | 2015-10-06 | Ibis Biosciences, Inc. | Targeted whole genome amplification method for identification of pathogens |
US9194877B2 (en) | 2009-07-17 | 2015-11-24 | Ibis Biosciences, Inc. | Systems for bioagent indentification |
US9393564B2 (en) | 2009-03-30 | 2016-07-19 | Ibis Biosciences, Inc. | Bioagent detection systems, devices, and methods |
US9416409B2 (en) | 2009-07-31 | 2016-08-16 | Ibis Biosciences, Inc. | Capture primers and capture sequence linked solid supports for molecular diagnostic tests |
US9598724B2 (en) | 2007-06-01 | 2017-03-21 | Ibis Biosciences, Inc. | Methods and compositions for multiple displacement amplification of nucleic acids |
US9873906B2 (en) | 2004-07-14 | 2018-01-23 | Ibis Biosciences, Inc. | Methods for repairing degraded DNA |
US9970061B2 (en) | 2011-12-27 | 2018-05-15 | Ibis Biosciences, Inc. | Bioagent detection oligonucleotides |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8076104B2 (en) * | 2007-01-25 | 2011-12-13 | Rogan Peter K | Rapid and comprehensive identification of prokaryotic organisms |
US7811766B2 (en) | 2007-03-28 | 2010-10-12 | Thinkvillage, Llc | Genetic identification and validation of Echinacea species |
US8527207B2 (en) * | 2007-05-15 | 2013-09-03 | Peter K. Rogan | Accurate identification of organisms based on individual information content |
JP5475344B2 (en) * | 2009-06-26 | 2014-04-16 | 株式会社日立ハイテクノロジーズ | Ion source apparatus, ionization probe manufacturing method, and ion source apparatus driving method |
WO2013022779A1 (en) * | 2011-08-05 | 2013-02-14 | Ibis Biosciences, Inc. | Analysis of genetic biomarkers for forensic analysis and fingerprinting |
JP5899731B2 (en) | 2011-09-13 | 2016-04-06 | ソニー株式会社 | Nucleic acid purification method, nucleic acid extraction method, and nucleic acid purification kit |
JP7026138B2 (en) * | 2017-12-26 | 2022-02-25 | 株式会社日立ハイテク | Automatic analyzer and analysis method |
CN112649489A (en) * | 2020-09-27 | 2021-04-13 | 浙江迪谱诊断技术有限公司 | Ion purification method for nucleic acid mass spectrum |
CN115774226A (en) * | 2021-09-08 | 2023-03-10 | 通用电气精准医疗有限责任公司 | Magnetic resonance imaging system, transmitting device and transmitting method |
Family Cites Families (410)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4075475A (en) * | 1976-05-03 | 1978-02-21 | Chemetron Corporation | Programmed thermal degradation-mass spectrometry analysis method facilitating identification of a biological specimen |
US5288611A (en) * | 1983-01-10 | 1994-02-22 | Gen-Probe Incorporated | Method for detecting, identifying, and quantitating organisms and viruses |
US5688645A (en) | 1983-01-10 | 1997-11-18 | Gen-Probe Incorporated | Method for detecting, identifying, and quantitating non-viral organisms |
US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
WO1988003957A1 (en) | 1986-11-24 | 1988-06-02 | Gen-Probe Incorporated | Nucleic acid probes for detection and/or quantitation of non-viral organisms |
US5188963A (en) | 1989-11-17 | 1993-02-23 | Gene Tec Corporation | Device for processing biological specimens for analysis of nucleic acids |
US5270030A (en) | 1988-12-29 | 1993-12-14 | Bio-Technology General Corp. | Fibrin binding domain polypeptide and method of producing |
US5198543A (en) | 1989-03-24 | 1993-03-30 | Consejo Superior Investigaciones Cientificas | PHI29 DNA polymerase |
WO1990015157A1 (en) | 1989-05-31 | 1990-12-13 | Gene-Trak Systems | Universal eubacteria nucleic acid probes and methods |
US5219727A (en) | 1989-08-21 | 1993-06-15 | Hoffmann-Laroche Inc. | Quantitation of nucleic acids using the polymerase chain reaction |
US5192659A (en) * | 1989-08-25 | 1993-03-09 | Genetype Ag | Intron sequence analysis method for detection of adjacent and remote locus alleles as haplotypes |
US5213961A (en) | 1989-08-31 | 1993-05-25 | Brigham And Women's Hospital | Accurate quantitation of RNA and DNA by competetitive polymerase chain reaction |
EP0667900B1 (en) | 1990-01-12 | 2001-05-23 | HSC Research Development Corporation | Introns and exons of the cystic fibrosis gene and mutations at various positions of the gene |
US5770029A (en) | 1996-07-30 | 1998-06-23 | Soane Biosciences | Integrated electrophoretic microdevices |
US5143905A (en) | 1990-05-03 | 1992-09-01 | The Regents Of The University Of California | Method and means for extending the host range of insecticidal proteins |
US5015845A (en) | 1990-06-01 | 1991-05-14 | Vestec Corporation | Electrospray method for mass spectrometry |
US5712125A (en) * | 1990-07-24 | 1998-01-27 | Cemv Bioteknik Ab | Competitive PCR for quantitation of DNA |
DE4030262A1 (en) | 1990-09-25 | 1992-03-26 | Suedzucker Ag | METHOD FOR PRODUCING RHAMNOSE FROM RHAMNOLIPID |
NL9002259A (en) | 1990-10-17 | 1992-05-18 | Eurodiagnostics B V | METHOD FOR DETERMINING A GENOTYPE BY COMPARING THE NUCLEOTID SEQUENCE OF MEM FAMILY MEMBERS AND KIT FOR DETECTING GENETIC VARIATIONS. |
WO1992009703A1 (en) | 1990-11-26 | 1992-06-11 | Cbr Laboratories, Inc. | Testing for spirochetal nucleic acid sequences in samples |
US5072115A (en) | 1990-12-14 | 1991-12-10 | Finnigan Corporation | Interpretation of mass spectra of multiply charged ions of mixtures |
WO1992013629A1 (en) | 1991-01-31 | 1992-08-20 | Wayne State University | A method for analyzing an organic sample |
US5866429A (en) * | 1991-04-03 | 1999-02-02 | Bloch; Will | Precision and accuracy of anion-exchange separation of nucleic acids |
US5472843A (en) | 1991-04-25 | 1995-12-05 | Gen-Probe Incorporated | Nucleic acid probes to Haemophilus influenzae |
US5213796A (en) | 1991-05-06 | 1993-05-25 | Dana Farber Cancer Institute | Assay for polyomavirus in humans and uses thereof |
US6055487A (en) * | 1991-07-30 | 2000-04-25 | Margery; Keith S. | Interactive remote sample analysis system |
CA2116543C (en) | 1991-07-31 | 2007-10-02 | Kay S. Greisen | Methods and reagents for detection of bacteria in cerebrospinal fluid |
ES2214472T3 (en) | 1991-08-02 | 2004-09-16 | Biomerieux B.V. | QUANTIFICATION OF NUCLEIC ACIDS. |
PT787807E (en) | 1991-08-27 | 2003-08-29 | Hoffmann La Roche | INITIATORS AND PROBES FOR HEPATITIS C DETECTION |
AU2580892A (en) | 1991-09-05 | 1993-04-05 | Isis Pharmaceuticals, Inc. | Determination of oligonucleotides for therapeutics, diagnostics and research reagents |
DE69231646T2 (en) | 1991-10-23 | 2001-06-13 | Baylor College Of Medicine, Houston | FINGERPRINT-LIKE IDENTIFICATION OF BACTERIA STRAINS BY AMPLIFICATING REPETITIVE DNA SEQUENCES |
FR2683827B1 (en) | 1991-11-15 | 1994-03-04 | Institut Nal Sante Recherc Medic | METHOD FOR DETERMINING THE QUANTITY OF A FRAGMENT OF DNA OF INTEREST BY AN ENZYMATIC AMPLIFICATION METHOD. |
US5484908A (en) * | 1991-11-26 | 1996-01-16 | Gilead Sciences, Inc. | Oligonucleotides containing 5-propynyl pyrimidines |
TW393513B (en) | 1991-11-26 | 2000-06-11 | Isis Pharmaceuticals Inc | Enhanced triple-helix and double-helix formation with oligomers containing modified pyrimidines |
JP3739785B2 (en) | 1991-11-26 | 2006-01-25 | アイシス ファーマシューティカルズ,インコーポレイティド | Enhanced triple and double helix shaping using oligomers containing modified pyrimidines |
US6235887B1 (en) * | 1991-11-26 | 2001-05-22 | Isis Pharmaceuticals, Inc. | Enhanced triple-helix and double-helix formation directed by oligonucleotides containing modified pyrimidines |
IL103935A0 (en) | 1991-12-04 | 1993-05-13 | Du Pont | Method for the identification of microorganisms by the utilization of directed and arbitrary dna amplification |
AU3794893A (en) | 1992-03-13 | 1993-10-05 | Park Scientific Instruments Corp. | Scanning probe microscope |
US5981176A (en) | 1992-06-17 | 1999-11-09 | City Of Hope | Method of detecting and discriminating between nucleic acid sequences |
US6303297B1 (en) | 1992-07-17 | 2001-10-16 | Incyte Pharmaceuticals, Inc. | Database for storage and analysis of full-length sequences |
FR2694754B1 (en) | 1992-08-12 | 1994-09-16 | Bio Merieux | Mycobacteria DNA fragments, amplification primers, hybridization probes, reagents and method for detecting detection of mycobacteria. |
AU5128593A (en) | 1992-09-16 | 1994-04-12 | University Of Tennessee Research Corporation, The | Antigen of hybrid m protein and carrier for group a streptococcal vaccine |
AU5298393A (en) | 1992-10-08 | 1994-05-09 | Regents Of The University Of California, The | Pcr assays to determine the presence and concentration of a target |
US5503980A (en) * | 1992-11-06 | 1996-04-02 | Trustees Of Boston University | Positional sequencing by hybridization |
GB9223334D0 (en) * | 1992-11-06 | 1992-12-23 | Hybaid Ltd | Magnetic solid phase supports |
US6436635B1 (en) | 1992-11-06 | 2002-08-20 | Boston University | Solid phase sequencing of double-stranded nucleic acids |
US6372424B1 (en) * | 1995-08-30 | 2002-04-16 | Third Wave Technologies, Inc | Rapid detection and identification of pathogens |
JPH08509857A (en) | 1993-01-07 | 1996-10-22 | シーケノム・インコーポレーテッド | DNA sequencing method by mass spectrometry |
US6194144B1 (en) | 1993-01-07 | 2001-02-27 | Sequenom, Inc. | DNA sequencing by mass spectrometry |
US5605798A (en) * | 1993-01-07 | 1997-02-25 | Sequenom, Inc. | DNA diagnostic based on mass spectrometry |
FR2701961B1 (en) | 1993-02-24 | 1995-04-21 | Bio Merieux | Method for destabilizing an intracatenary secondary structure of a single-stranded polynucleotide, and for capturing said nucleotide. |
US6074823A (en) | 1993-03-19 | 2000-06-13 | Sequenom, Inc. | DNA sequencing by mass spectrometry via exonuclease degradation |
EP0689610B1 (en) | 1993-03-19 | 2002-07-03 | Sequenom, Inc. | Dna sequencing by mass spectrometry via exonuclease degradation |
US5639606A (en) | 1993-04-06 | 1997-06-17 | The University Of Rochester | Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chain reaction |
US6323041B1 (en) * | 1993-06-11 | 2001-11-27 | Pfizer Inc. | Screening novel human phosphodiesterase IV isozymes for compounds which modify their enzymatic activity |
US5830853A (en) | 1994-06-23 | 1998-11-03 | Astra Aktiebolag | Systemic administration of a therapeutic preparation |
US6001584A (en) | 1993-07-19 | 1999-12-14 | The Regents Of The University Of California | Oncoprotein protein kinase |
AU7551594A (en) | 1993-07-29 | 1995-02-28 | MURASHIGE, Kate H. | Method for recognition of the nucleotide sequence of a purified dna segment |
GB9315847D0 (en) | 1993-07-30 | 1993-09-15 | Isis Innovation | Tag reagent and assay method |
US5527675A (en) | 1993-08-20 | 1996-06-18 | Millipore Corporation | Method for degradation and sequencing of polymers which sequentially eliminate terminal residues |
US6376178B1 (en) * | 1993-09-03 | 2002-04-23 | Duke University | Method of nucleic acid sequencing |
WO1995006752A1 (en) | 1993-09-03 | 1995-03-09 | Duke University | A method of nucleic acid sequencing |
US5502177A (en) | 1993-09-17 | 1996-03-26 | Gilead Sciences, Inc. | Pyrimidine derivatives for labeled binding partners |
WO1995011996A1 (en) | 1993-10-27 | 1995-05-04 | Cornell Research Foundation, Inc. | Detection assay for listeria and erwinia microorganisms |
US5504327A (en) * | 1993-11-04 | 1996-04-02 | Hv Ops, Inc. (H-Nu) | Electrospray ionization source and method for mass spectrometric analysis |
DE4338119A1 (en) | 1993-11-08 | 1995-05-11 | Bayer Ag | Specific gene probes and methods for the quantitative detection of methicillin-resistant staphylococci |
NL9301957A (en) | 1993-11-11 | 1995-06-01 | U Gene Research Bv | Method for identifying microorganisms, and useful tools. |
US5928905A (en) | 1995-04-18 | 1999-07-27 | Glaxo Group Limited | End-complementary polymerase reaction |
JP3396942B2 (en) | 1994-02-21 | 2003-04-14 | 三菱化学株式会社 | Method for producing aromatic polycarbonate |
US5849492A (en) | 1994-02-28 | 1998-12-15 | Phylogenetix Laboratories, Inc. | Method for rapid identification of prokaryotic and eukaryotic organisms |
DE4444229C2 (en) * | 1994-03-10 | 1996-07-25 | Bruker Franzen Analytik Gmbh | Methods and devices for electrospray ionization for storage mass spectrometers |
US5608217A (en) * | 1994-03-10 | 1997-03-04 | Bruker-Franzen Analytik Gmbh | Electrospraying method for mass spectrometric analysis |
US5976798A (en) | 1994-03-30 | 1999-11-02 | Mitokor | Methods for detecting mitochondrial mutations diagnostic for Alzheimer's disease and methods for determining heteroplasmy of mitochondrial nucleic acid |
WO1995031997A1 (en) | 1994-05-20 | 1995-11-30 | UNITED STATES OF AMERICA, represented by THE SECRETARY OF THE ARMY | Model for testing immunogenicity of peptides |
US5814442A (en) | 1994-06-10 | 1998-09-29 | Georgetown University | Internally controlled virion nucleic acid amplification reaction for quantitation of virion and virion nucleic acid |
DE4421901A1 (en) * | 1994-06-23 | 1996-01-04 | Bayer Ag | A rapid DNA test for the detection of quinolone-resistant Staphylococcus aureus pathogens in clinical specimens |
US5541405A (en) | 1994-07-27 | 1996-07-30 | Parker-Hannifin Corporation | Method and device for continuous pattern sensing using fiber optics |
GB9417211D0 (en) | 1994-08-25 | 1994-10-12 | Solicitor For The Affairs Of H | Nucleotide sequencing method |
US20020055101A1 (en) | 1995-09-11 | 2002-05-09 | Michel G. Bergeron | Specific and universal probes and amplification primers to rapidly detect and identify common bacterial pathogens and antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories |
US6001564A (en) | 1994-09-12 | 1999-12-14 | Infectio Diagnostic, Inc. | Species specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial pathogens and associated antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories |
CA2118048C (en) * | 1994-09-30 | 2003-04-08 | James W. Schumm | Multiplex amplification of short tandem repeat loci |
US5753489A (en) | 1994-11-10 | 1998-05-19 | Immuno Ag | Method for producing viruses and vaccines in serum-free culture |
US5654141A (en) | 1994-11-18 | 1997-08-05 | Thomas Jefferson University | Amplification based detection of bacterial infection |
KR100399813B1 (en) | 1994-12-14 | 2004-06-09 | 가부시키가이샤 니콘 | Exposure apparatus |
US6180339B1 (en) * | 1995-01-13 | 2001-01-30 | Bayer Corporation | Nucleic acid probes for the detection and identification of fungi |
US5763169A (en) | 1995-01-13 | 1998-06-09 | Chiron Diagnostics Corporation | Nucleic acid probes for the detection and identification of fungi |
US5707802A (en) * | 1995-01-13 | 1998-01-13 | Ciba Corning Diagnostics Corp. | Nucleic acid probes for the detection and identification of fungi |
US5702895A (en) | 1995-01-19 | 1997-12-30 | Wakunaga Seiyaku Kabushiki Kaisha | Method and kit for detecting methicillin-resistant Staphylococcus aureus |
US5484808A (en) * | 1995-02-09 | 1996-01-16 | Eli Lilly And Company | Methods of inhibiting cell-cell adhesion |
GB9504598D0 (en) | 1995-03-03 | 1995-04-26 | Imp Cancer Res Tech | Method of nucleic acid analysis |
US6428955B1 (en) | 1995-03-17 | 2002-08-06 | Sequenom, Inc. | DNA diagnostics based on mass spectrometry |
EP0830460A1 (en) | 1995-04-11 | 1998-03-25 | Trustees Of Boston University | Solid phase sequencing of biopolymers |
US5932220A (en) | 1995-05-08 | 1999-08-03 | Board Of Regents University Of Texas System | Diagnostic tests for a new spirochete, Borrelia lonestari sp. nov. |
US5625184A (en) * | 1995-05-19 | 1997-04-29 | Perseptive Biosystems, Inc. | Time-of-flight mass spectrometry analysis of biomolecules |
US5830655A (en) | 1995-05-22 | 1998-11-03 | Sri International | Oligonucleotide sizing using cleavable primers |
US5700642A (en) | 1995-05-22 | 1997-12-23 | Sri International | Oligonucleotide sizing using immobilized cleavable primers |
AU708995B2 (en) | 1995-06-07 | 1999-08-19 | Gene Shears Pty. Limited | Optimized minizymes and miniribozymes and uses thereof |
US5856174A (en) * | 1995-06-29 | 1999-01-05 | Affymetrix, Inc. | Integrated nucleic acid diagnostic device |
US6218529B1 (en) * | 1995-07-31 | 2001-04-17 | Urocor, Inc. | Biomarkers and targets for diagnosis, prognosis and management of prostate, breast and bladder cancer |
US6146854A (en) | 1995-08-31 | 2000-11-14 | Sequenom, Inc. | Filtration processes, kits and devices for isolating plasmids |
US5994066A (en) | 1995-09-11 | 1999-11-30 | Infectio Diagnostic, Inc. | Species-specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial pathogens and associated antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories |
US5869242A (en) * | 1995-09-18 | 1999-02-09 | Myriad Genetics, Inc. | Mass spectrometry to assess DNA sequence polymorphisms |
US5727202A (en) | 1995-10-18 | 1998-03-10 | Palm Computing, Inc. | Method and apparatus for synchronizing information on two different computer systems |
US5871697A (en) * | 1995-10-24 | 1999-02-16 | Curagen Corporation | Method and apparatus for identifying, classifying, or quantifying DNA sequences in a sample without sequencing |
US5972693A (en) | 1995-10-24 | 1999-10-26 | Curagen Corporation | Apparatus for identifying, classifying, or quantifying DNA sequences in a sample without sequencing |
US5716825A (en) * | 1995-11-01 | 1998-02-10 | Hewlett Packard Company | Integrated nucleic acid analysis system for MALDI-TOF MS |
WO1997023646A1 (en) * | 1995-12-22 | 1997-07-03 | Behringwerke Aktiengesellschaft | Detection of differences in nucleic acids |
US6312893B1 (en) | 1996-01-23 | 2001-11-06 | Qiagen Genomics, Inc. | Methods and compositions for determining the sequence of nucleic acid molecules |
GB9602028D0 (en) | 1996-02-01 | 1996-04-03 | Amersham Int Plc | Nucleoside analogues |
US6852487B1 (en) * | 1996-02-09 | 2005-02-08 | Cornell Research Foundation, Inc. | Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays |
EP0886681A1 (en) | 1996-03-04 | 1998-12-30 | Genetrace Systems, Inc. | Methods of screening nucleic acids using mass spectrometry |
AU2217597A (en) | 1996-03-18 | 1997-10-22 | Sequenom, Inc. | Dna sequencing by mass spectrometry |
CA2222192A1 (en) | 1996-03-20 | 1997-09-25 | Bio Merieux | Nucleic acid isolation |
JP3365198B2 (en) | 1996-03-21 | 2003-01-08 | ミノルタ株式会社 | Image forming device |
US5745751A (en) * | 1996-04-12 | 1998-04-28 | Nelson; Robert W. | Civil site information system |
US6214555B1 (en) * | 1996-05-01 | 2001-04-10 | Visible Genetics Inc. | Method compositions and kit for detection |
US5928906A (en) | 1996-05-09 | 1999-07-27 | Sequenom, Inc. | Process for direct sequencing during template amplification |
EP0954611A1 (en) | 1996-06-10 | 1999-11-10 | University Of Utah Research Foundation | Rapid, accurate identification of dna sequence variants by electrospray mass spectrometry |
WO1998003684A1 (en) | 1996-07-19 | 1998-01-29 | Hybridon, Inc. | Method for sequencing nucleic acids using matrix-assisted laser desorption ionization time-of-flight mass spectrometry |
US6563025B1 (en) | 1996-07-26 | 2003-05-13 | Board Of Trustees Of The University Of Illinois | Nucleotide sequences encoding anthranilate synthase |
US5831046A (en) * | 1996-08-05 | 1998-11-03 | Prolinx, Incorporated | Boronic acid-contaning nucleic acid monomers |
DE19633436A1 (en) | 1996-08-20 | 1998-02-26 | Boehringer Mannheim Gmbh | Method for the detection of nucleic acids by determining the mass |
CA2301875A1 (en) | 1996-09-19 | 1998-03-26 | Genetrace Systems | Methods of preparing nucleic acids for mass spectrometric analysis |
US5777324A (en) | 1996-09-19 | 1998-07-07 | Sequenom, Inc. | Method and apparatus for maldi analysis |
US5965363A (en) | 1996-09-19 | 1999-10-12 | Genetrace Systems Inc. | Methods of preparing nucleic acids for mass spectrometric analysis |
US6361940B1 (en) * | 1996-09-24 | 2002-03-26 | Qiagen Genomics, Inc. | Compositions and methods for enhancing hybridization and priming specificity |
US5864137A (en) * | 1996-10-01 | 1999-01-26 | Genetrace Systems, Inc. | Mass spectrometer |
GB9620769D0 (en) | 1996-10-04 | 1996-11-20 | Brax Genomics Ltd | Nucleic acid sequencing |
US5885775A (en) * | 1996-10-04 | 1999-03-23 | Perseptive Biosystems, Inc. | Methods for determining sequences information in polynucleotides using mass spectrometry |
US6110710A (en) | 1996-10-15 | 2000-08-29 | The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Sequence modification of oligonucleotide primers to manipulate non-templated nucleotide addition |
US20030129589A1 (en) * | 1996-11-06 | 2003-07-10 | Hubert Koster | Dna diagnostics based on mass spectrometry |
US6024925A (en) * | 1997-01-23 | 2000-02-15 | Sequenom, Inc. | Systems and methods for preparing low volume analyte array elements |
US6133436A (en) | 1996-11-06 | 2000-10-17 | Sequenom, Inc. | Beads bound to a solid support and to nucleic acids |
US6140053A (en) | 1996-11-06 | 2000-10-31 | Sequenom, Inc. | DNA sequencing by mass spectrometry via exonuclease degradation |
EP1457496B1 (en) | 1996-11-06 | 2014-01-15 | Sequenom, Inc. | High density immobilization of nucleic acids |
US7285422B1 (en) | 1997-01-23 | 2007-10-23 | Sequenom, Inc. | Systems and methods for preparing and analyzing low volume analyte array elements |
US5900481A (en) | 1996-11-06 | 1999-05-04 | Sequenom, Inc. | Bead linkers for immobilizing nucleic acids to solid supports |
US6060246A (en) | 1996-11-15 | 2000-05-09 | Avi Biopharma, Inc. | Reagent and method for isolation and detection of selected nucleic acid sequences |
KR20000057275A (en) * | 1996-11-28 | 2000-09-15 | 후지타 기요시 | Sugar compounds, gelling agents, gelling agent compositions, processes for the preparation of them, and gel compositions |
US5822824A (en) | 1996-12-03 | 1998-10-20 | Dion; William D. | Mountable washing device |
AU5794498A (en) | 1996-12-10 | 1998-07-03 | Genetrace Systems, Inc. | Releasable nonvolatile mass-label molecules |
US5981190A (en) | 1997-01-08 | 1999-11-09 | Ontogeny, Inc. | Analysis of gene expression, methods and reagents therefor |
US5876936A (en) * | 1997-01-15 | 1999-03-02 | Incyte Pharmaceuticals, Inc. | Nucleic acid sequencing with solid phase capturable terminators |
US6046005A (en) * | 1997-01-15 | 2000-04-04 | Incyte Pharmaceuticals, Inc. | Nucleic acid sequencing with solid phase capturable terminators comprising a cleavable linking group |
ATE340869T1 (en) | 1997-01-15 | 2006-10-15 | Xzillion Gmbh & Co Kg | MASS MARKED HYBRIDIZATION PROBE |
WO1998035057A1 (en) | 1997-02-06 | 1998-08-13 | The National University Of Singapore | Diagnosis of plasmodium infection by analysis of extrachromosomal genetic material |
US6727061B2 (en) | 1997-02-20 | 2004-04-27 | Cabtec, Inc. | Methods for identifying species or Shigella and E. coli using operon sequence analysis |
US5828062A (en) | 1997-03-03 | 1998-10-27 | Waters Investments Limited | Ionization electrospray apparatus for mass spectrometry |
US6553317B1 (en) | 1997-03-05 | 2003-04-22 | Incyte Pharmaceuticals, Inc. | Relational database and system for storing information relating to biomolecular sequences and reagents |
DE19710166C1 (en) | 1997-03-12 | 1998-12-10 | Bruker Franzen Analytik Gmbh | Two-step method of DNA amplification for MALDI-TOF measurements |
AU6553498A (en) | 1997-03-14 | 1998-09-29 | Hybridon, Inc. | Method for sequencing of modified nucleic acids using electrospray ionization-fourier transform mass spectrometry |
US5849497A (en) | 1997-04-03 | 1998-12-15 | The Research Foundation Of State University Of New York | Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker |
US6018713A (en) * | 1997-04-09 | 2000-01-25 | Coli; Robert D. | Integrated system and method for ordering and cumulative results reporting of medical tests |
DE19717085C2 (en) * | 1997-04-23 | 1999-06-17 | Bruker Daltonik Gmbh | Processes and devices for extremely fast DNA multiplication using polymerase chain reactions (PCR) |
US20010039263A1 (en) | 1997-05-02 | 2001-11-08 | Max-Delbruck-Centrum Fur Molekulare Medizin | Chimeric oligonucleotides and the use thereof |
US6054278A (en) * | 1997-05-05 | 2000-04-25 | The Perkin-Elmer Corporation | Ribosomal RNA gene polymorphism based microorganism identification |
US6159681A (en) | 1997-05-28 | 2000-12-12 | Syntrix Biochip, Inc. | Light-mediated method and apparatus for the regional analysis of biologic material |
US6994960B1 (en) | 1997-05-28 | 2006-02-07 | The Walter And Eliza Hall Institute Of Medical Research | Nucleic acid diagnostics based on mass spectrometry or mass separation and base specific cleavage |
WO1998054751A1 (en) | 1997-05-30 | 1998-12-03 | Genetrace Systems, Inc. | Volatile matrices for matrix-assisted laser desorption/ionization mass spectrometry |
US6061686A (en) | 1997-06-26 | 2000-05-09 | Digital Equipment Corporation | Updating a copy of a remote document stored in a local computer system |
NZ501919A (en) | 1997-07-22 | 2001-11-30 | Qiagen Genomics Inc | Methods for analyzing nucleic acid molecules utilizing mass spectroscopy readable tags |
DE19732086C2 (en) | 1997-07-25 | 2002-11-21 | Univ Leipzig | Method for the quantitative determination of eubacteria |
US6207370B1 (en) | 1997-09-02 | 2001-03-27 | Sequenom, Inc. | Diagnostics based on mass spectrometric detection of translated target polypeptides |
GB9719044D0 (en) | 1997-09-08 | 1997-11-12 | Inst Of Ophthalmology | Assay |
US6090558A (en) | 1997-09-19 | 2000-07-18 | Genetrace Systems, Inc. | DNA typing by mass spectrometry with polymorphic DNA repeat markers |
US6063031A (en) | 1997-10-14 | 2000-05-16 | Assurance Medical, Inc. | Diagnosis and treatment of tissue with instruments |
US6111096A (en) | 1997-10-31 | 2000-08-29 | Bbi Bioseq, Inc. | Nucleic acid isolation and purification |
JP3423597B2 (en) | 1997-11-05 | 2003-07-07 | 三井農林株式会社 | Bacterial identification method |
US6028183A (en) * | 1997-11-07 | 2000-02-22 | Gilead Sciences, Inc. | Pyrimidine derivatives and oligonucleotides containing same |
US6007992A (en) | 1997-11-10 | 1999-12-28 | Gilead Sciences, Inc. | Pyrimidine derivatives for labeled binding partners |
WO1999029898A2 (en) | 1997-12-05 | 1999-06-17 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Method for identifying nucleic acids by means of matrix-assisted laser desorption/ionisation mass spectrometry |
US6914137B2 (en) * | 1997-12-06 | 2005-07-05 | Dna Research Innovations Limited | Isolation of nucleic acids |
US6268131B1 (en) | 1997-12-15 | 2001-07-31 | Sequenom, Inc. | Mass spectrometric methods for sequencing nucleic acids |
US6458533B1 (en) | 1997-12-19 | 2002-10-01 | High Throughput Genomics, Inc. | High throughput assay system for monitoring ESTs |
GB9815166D0 (en) | 1998-07-13 | 1998-09-09 | Brax Genomics Ltd | Compounds for mass spectrometry |
US20030096232A1 (en) | 1997-12-19 | 2003-05-22 | Kris Richard M. | High throughput assay system |
JP3793342B2 (en) | 1997-12-26 | 2006-07-05 | ソニーケミカル株式会社 | Acrylic emulsion and adhesive tape |
DE19802905C2 (en) | 1998-01-27 | 2001-11-08 | Bruker Daltonik Gmbh | Process for the preferred production of only one strand of selected genetic material for mass spectrometric measurements |
US6428956B1 (en) | 1998-03-02 | 2002-08-06 | Isis Pharmaceuticals, Inc. | Mass spectrometric methods for biomolecular screening |
WO1999046047A2 (en) | 1998-03-10 | 1999-09-16 | Large Scale Proteomics Corporation | Detection and characterization of microorganisms |
US6235480B1 (en) | 1998-03-13 | 2001-05-22 | Promega Corporation | Detection of nucleic acid hybrids |
US6268146B1 (en) | 1998-03-13 | 2001-07-31 | Promega Corporation | Analytical methods and materials for nucleic acid detection |
US6391551B1 (en) | 1998-03-13 | 2002-05-21 | Promega Corporation | Detection of nucleic acid hybrids |
US6270973B1 (en) | 1998-03-13 | 2001-08-07 | Promega Corporation | Multiplex method for nucleic acid detection |
US6270974B1 (en) | 1998-03-13 | 2001-08-07 | Promega Corporation | Exogenous nucleic acid detection |
US6277578B1 (en) | 1998-03-13 | 2001-08-21 | Promega Corporation | Deploymerization method for nucleic acid detection of an amplified nucleic acid target |
US6312902B1 (en) | 1998-03-13 | 2001-11-06 | Promega Corporation | Nucleic acid detection |
US6261769B1 (en) | 1998-03-31 | 2001-07-17 | The United States Of America As Represented By The Secretary Of Agriculture | Intergenic spacer target sequence for detecting and distinguishing Chlamydial species or strains |
US20030228597A1 (en) | 1998-04-13 | 2003-12-11 | Cowsert Lex M. | Identification of genetic targets for modulation by oligonucleotides and generation of oligonucleotides for gene modulation |
US7321828B2 (en) * | 1998-04-13 | 2008-01-22 | Isis Pharmaceuticals, Inc. | System of components for preparing oligonucleotides |
US6223186B1 (en) | 1998-05-04 | 2001-04-24 | Incyte Pharmaceuticals, Inc. | System and method for a precompiled database for biomolecular sequence information |
US6723564B2 (en) * | 1998-05-07 | 2004-04-20 | Sequenom, Inc. | IR MALDI mass spectrometry of nucleic acids using liquid matrices |
DE19822108A1 (en) | 1998-05-12 | 2000-02-03 | Schering Ag | Method for the detection of microorganisms in products, in particular in medicines and cosmetics |
US6221587B1 (en) * | 1998-05-12 | 2001-04-24 | Isis Pharmceuticals, Inc. | Identification of molecular interaction sites in RNA for novel drug discovery |
DE19922161A1 (en) | 1998-05-18 | 1999-12-09 | Fraunhofer Ges Forschung | Anti-adhesion coating for e.g. soldering/welding tools and electric contacts |
US6468743B1 (en) | 1998-05-18 | 2002-10-22 | Conagra Grocery Products Company | PCR techniques for detecting microbial contaminants in foodstuffs |
DE19824280B4 (en) | 1998-05-29 | 2004-08-19 | Bruker Daltonik Gmbh | Mutation analysis using mass spectrometry |
US6104028A (en) | 1998-05-29 | 2000-08-15 | Genetrace Systems Inc. | Volatile matrices for matrix-assisted laser desorption/ionization mass spectrometry |
GB2339905A (en) | 1998-06-24 | 2000-02-09 | Bruker Daltonik Gmbh | Use of mass-specrometry for detection of mutations |
DE69941333D1 (en) | 1998-07-02 | 2009-10-08 | Gen Probe Inc | MOLECULAR TORCHES |
US6218118B1 (en) * | 1998-07-09 | 2001-04-17 | Agilent Technologies, Inc. | Method and mixture reagents for analyzing the nucleotide sequence of nucleic acids by mass spectrometry |
US6074831A (en) | 1998-07-09 | 2000-06-13 | Agilent Technologies, Inc. | Partitioning of polymorphic DNAs |
US6432651B1 (en) | 1998-07-10 | 2002-08-13 | Cetek Corporation | Method to detect and analyze tight-binding ligands in complex biological samples using capillary electrophoresis and mass spectrometry |
US6605433B1 (en) | 1998-08-20 | 2003-08-12 | The Johns Hopkins University | Mitochondrial dosimeter |
US6146144A (en) | 1998-09-29 | 2000-11-14 | Fowler; Ernest R. | Rug hooking kit and method for handicapped |
US6610492B1 (en) | 1998-10-01 | 2003-08-26 | Variagenics, Inc. | Base-modified nucleotides and cleavage of polynucleotides incorporating them |
US6238927B1 (en) | 1998-10-05 | 2001-05-29 | Mosaic Technologies, Incorporated | Reverse displacement assay for detection of nucleic acid sequences |
IT1303330B1 (en) | 1998-11-05 | 2000-11-06 | Ilpea Ind Spa | GASKET FOR FROGORIFERI WITH SHAPED COUNTERPORT. |
DE19852167C2 (en) | 1998-11-12 | 2000-12-14 | Bruker Saxonia Analytik Gmbh | Simple SNP analysis using mass spectrometry |
EP1144594A1 (en) | 1998-11-24 | 2001-10-17 | Regents Of The University Of Minnesota | Transgenic circulating endothelial cells |
US6994962B1 (en) * | 1998-12-09 | 2006-02-07 | Massachusetts Institute Of Technology | Methods of identifying point mutations in a genome |
DE19859723A1 (en) | 1998-12-23 | 2000-06-29 | Henkel Kgaa | Preparations for coloring keratinous fibers |
US7432049B2 (en) | 2001-02-28 | 2008-10-07 | Chondrogene Limited | Compositions and methods relating to osteoarthritis |
US6503718B2 (en) | 1999-01-10 | 2003-01-07 | Exact Sciences Corporation | Methods for detecting mutations using primer extension for detecting disease |
US6638714B1 (en) | 1999-02-03 | 2003-10-28 | Ortho-Clinical Diagnostics, Inc. | Oligonucleotide primers for efficient detection of hepatitis C virus (HCV) and methods of use thereof |
US6153389A (en) | 1999-02-22 | 2000-11-28 | Haarer; Brian K. | DNA additives as a mechanism for unambiguously marking biological samples |
EP1035219A1 (en) | 1999-02-25 | 2000-09-13 | Universiteit Gent | Gastric helicobacter 16 S rDNA sequences from cattle and pigs and their use for detection and typing of Helicobacter strains |
DE19910592A1 (en) * | 1999-03-10 | 2000-09-14 | Volkswagen Ag | Restraint |
US6436640B1 (en) | 1999-03-18 | 2002-08-20 | Exiqon A/S | Use of LNA in mass spectrometry |
US6613509B1 (en) | 1999-03-22 | 2003-09-02 | Regents Of The University Of California | Determination of base (nucleotide) composition in DNA oligomers by mass spectrometry |
EP1171584A1 (en) | 1999-04-21 | 2002-01-16 | Annovis, Inc. | Magnetic dna extraction kit for plants |
US6649351B2 (en) | 1999-04-30 | 2003-11-18 | Aclara Biosciences, Inc. | Methods for detecting a plurality of analytes by mass spectrometry |
AU778230B2 (en) | 1999-05-03 | 2004-11-25 | Gen-Probe Incorporated | Polynucleotide matrix-based method of identifying microorganisms |
US20020086289A1 (en) | 1999-06-15 | 2002-07-04 | Don Straus | Genomic profiling: a rapid method for testing a complex biological sample for the presence of many types of organisms |
MXPA02000192A (en) | 1999-06-30 | 2004-08-12 | Corixa Corp | Compositions and methods for the therapy and diagnosis of lung cancer. |
AU5371099A (en) | 1999-07-22 | 2001-02-13 | Artus Gesellschaft Fur Molekularbiologische Diagnostik Und Entwicklung Mbh | Method for the species-specific detection of organisms |
US6723505B1 (en) | 1999-08-13 | 2004-04-20 | Nye Colifast As | Method for identification of the indicators of contamination in liquid samples |
US6266144B1 (en) | 1999-08-26 | 2001-07-24 | Taiwan Semiconductor Manufacturing Company | Stepper and scanner new exposure sequence with intra-field correction |
DE19943374A1 (en) | 1999-09-10 | 2001-03-29 | Max Planck Gesellschaft | Method for binding nucleic acids to a solid phase |
WO2001023608A2 (en) | 1999-09-27 | 2001-04-05 | Merck Sharp & Dohme De Espana, S.A.E. | Hybridization probes which specifically detect strains of the genera microbispora, microtetraspora, nonomuria and planobispora |
EP2322666A3 (en) | 1999-09-28 | 2011-08-10 | Geneohm Sciences Canada, Inc. | Highly conserved gene and its use to generate species-specific, genus-specific, family-specific, group-specific and universal nucleic acid probes for microorganisms. |
US6296188B1 (en) * | 1999-10-01 | 2001-10-02 | Perfect Plastic Printing Corporation | Transparent/translucent financial transaction card including an infrared light filter |
US6787302B2 (en) | 1999-10-25 | 2004-09-07 | Genprime, Inc. | Method and apparatus for prokaryotic and eukaryotic cell quantitation |
WO2001032930A1 (en) | 1999-11-04 | 2001-05-10 | California Institute Of Technology | Methods and apparatuses for analyzing polynucleotide sequences |
US6286146B1 (en) | 1999-11-15 | 2001-09-11 | Debra Rocker | Method of wearing weighted training vest while listening to audio equipment |
US6856914B1 (en) * | 1999-11-19 | 2005-02-15 | The University Of British Columbia | Method, apparatus, media and signals for identifying associated cell signaling proteins |
KR20020060242A (en) | 1999-11-29 | 2002-07-16 | 추후제출 | Method for obtaining nucleic acids from an environment sample, resulting nucleic acids and use in synthesis of novel compounds |
US6608190B1 (en) | 1999-12-16 | 2003-08-19 | E. I. Du Pont De Nemours And Company | Nucleic acid fragments for the identification of bacteria in industrial wastewater bioreactors |
US6936414B2 (en) | 1999-12-22 | 2005-08-30 | Abbott Laboratories | Nucleic acid isolation method and kit |
US7166429B2 (en) | 1999-12-29 | 2007-01-23 | Keygene N.V. | Method for generating oligonucleotides, in particular for the detection of amplified restriction fragments obtained using AFLP® |
SE0000061D0 (en) | 2000-01-10 | 2000-01-10 | Bjoern Herrmann | A method for detection of pathogenic organisms |
AU2001232485A1 (en) * | 2000-01-13 | 2001-07-24 | Amsterdam Support Diagnostics B.V. | A universal nucleic acid amplification system for nucleic acids in a sample |
US20020009727A1 (en) | 2000-02-02 | 2002-01-24 | Schultz Gary A. | Detection of single nucleotide polymorphisms |
CA2298181C (en) | 2000-02-02 | 2006-09-19 | Dayan Burke Goodnough | Non-targeted complex sample analysis |
US6453244B1 (en) | 2000-02-10 | 2002-09-17 | Stanford University | Detection of polymorphisms by denaturing high-performance liquid chromatography |
US6569093B2 (en) | 2000-02-14 | 2003-05-27 | First Opinion Corporation | Automated diagnostic system and method including disease timeline |
US6393367B1 (en) | 2000-02-19 | 2002-05-21 | Proteometrics, Llc | Method for evaluating the quality of comparisons between experimental and theoretical mass data |
DE10015797B4 (en) | 2000-03-26 | 2006-02-02 | Bruker Daltonik Gmbh | Multiplex analysis of DNA mixtures using photolytically readable DNA chips |
DE10015262A1 (en) | 2000-03-28 | 2001-10-04 | Basf Ag | Paper coating composition useful for off set printing, contains a binding agent prepared by radical polymerization of ethylenically unsaturated compounds |
AU6052001A (en) | 2000-03-29 | 2001-10-08 | Ct For The Applic Of Molecular | Methods for genotyping by hybridization analysis |
DK2278029T3 (en) | 2000-04-10 | 2016-10-03 | Taxon Biosciences Inc | Methods of study and genetic analysis of populations |
US6475736B1 (en) | 2000-05-23 | 2002-11-05 | Variagenics, Inc. | Methods for genetic analysis of DNA using biased amplification of polymorphic sites |
US6716634B1 (en) * | 2000-05-31 | 2004-04-06 | Agilent Technologies, Inc. | Increasing ionization efficiency in mass spectrometry |
US6507837B1 (en) | 2000-06-08 | 2003-01-14 | Hyperphrase Technologies, Llc | Tiered and content based database searching |
CA2412223A1 (en) | 2000-06-09 | 2001-12-20 | Corixa Corporation | Compositions and methods for the therapy and diagnosis of colon cancer |
AU2001268468A1 (en) * | 2000-06-13 | 2001-12-24 | The Trustees Of Boston University | Use of nucleotide analogs in the analysis of oligonucleotide mixtures and in highly multiplexed nucleic acid sequencing |
EP1170379A1 (en) * | 2000-06-30 | 2002-01-09 | Centre National de Genotypage | Sample generation for genotyping by mass spectrometry |
KR100762863B1 (en) * | 2000-06-30 | 2007-10-08 | 주식회사 하이닉스반도체 | METHOD OF FABRICATING COPPER-LINE UTILIZED Ti-Si-N FILM FOR PREVENTING DIFFUSION |
FR2811321A1 (en) | 2000-07-04 | 2002-01-11 | Bio Merieux | New oligonucleotide primers, useful for identifying bacteria, particularly in cases of septicemia, provide amplification of bacterial 16S ribosomal nucleic acid |
US6504021B2 (en) * | 2000-07-05 | 2003-01-07 | Edge Biosystems, Inc. | Ion exchange method for DNA purification |
WO2002002811A2 (en) | 2000-07-06 | 2002-01-10 | Bio Merieux | Method for controlling the microbiological quality of an aqueous medium and kit therefor |
US6783939B2 (en) | 2000-07-07 | 2004-08-31 | Alphavax, Inc. | Alphavirus vectors and virosomes with modified HIV genes for use in vaccines |
WO2002010186A1 (en) | 2000-07-27 | 2002-02-07 | California Institute Of Technology | A rapid, quantitative method for the mass spectrometric analysis of nucleic acids for gene expression and genotyping |
AUPQ909000A0 (en) | 2000-07-28 | 2000-08-24 | University Of Sydney, The | A method of detecting microorganisms |
GB0021286D0 (en) | 2000-08-30 | 2000-10-18 | Gemini Genomics Ab | Identification of drug metabolic capacity |
US20030190635A1 (en) | 2002-02-20 | 2003-10-09 | Mcswiggen James A. | RNA interference mediated treatment of Alzheimer's disease using short interfering RNA |
US20040005555A1 (en) * | 2000-08-31 | 2004-01-08 | Rothman Richard E. | Molecular diagnosis of bactermia |
US20020120408A1 (en) | 2000-09-06 | 2002-08-29 | Kreiswirth Barry N. | System and method for tracking and controlling infections |
US7349808B1 (en) * | 2000-09-06 | 2008-03-25 | Egenomics, Inc. | System and method for tracking and controlling infections |
US6813615B1 (en) | 2000-09-06 | 2004-11-02 | Cellomics, Inc. | Method and system for interpreting and validating experimental data with automated reasoning |
AU2001288762A1 (en) | 2000-09-08 | 2002-03-22 | Large Scale Proteomics Corporation | Detection and characterization of microorganisms |
SE0003286D0 (en) | 2000-09-15 | 2000-09-15 | Ulf Gyllensten | Method and kit for human identification |
ES2327103T3 (en) | 2000-09-25 | 2009-10-26 | Polymun Scientific Immunbiologische Forschung Gmbh | LIVE INFLUENZAVIRUS VACCINE AND MANUFACTURING PROCEDURE. |
WO2002028901A2 (en) | 2000-10-05 | 2002-04-11 | Millennium Pharmaceuticals, Inc. | Seven-transmembrane (7tm) receptors and uses thereof |
US6996472B2 (en) | 2000-10-10 | 2006-02-07 | The United States Of America As Represented By The Department Of Health And Human Services | Drift compensation method for fingerprint spectra |
AU2002213345A1 (en) | 2000-10-13 | 2002-04-22 | Irm, Llc | High throughput processing system and method of using |
ATE380883T1 (en) | 2000-10-24 | 2007-12-15 | Univ Leland Stanford Junior | DIRECT MULTIPLEX CHARACTERIZATION OF GENOMIC DNA |
US6906316B2 (en) | 2000-10-27 | 2005-06-14 | Fuji Electric Co., Ltd. | Semiconductor device module |
US6682889B1 (en) * | 2000-11-08 | 2004-01-27 | Becton, Dickinson And Company | Amplification and detection of organisms of the Chlamydiaceae family |
AU2002222614A1 (en) | 2000-12-12 | 2002-07-01 | Chugai Seiyaku Kabushiki Kaisha | Method of detecting polymorphism in dna by using mass spectroscopy |
US6800289B2 (en) | 2000-12-21 | 2004-10-05 | Her Majesty The Queen In Right Of Canada, As Represented By The Minister Of National Defence | Strain of the western equine encephalitis virus |
US6586584B2 (en) | 2001-01-29 | 2003-07-01 | Becton, Dickinson And Company | Sequences and methods for detection of Hepatitis C virus |
DE10108453B4 (en) | 2001-02-22 | 2005-10-20 | Bruker Daltonik Gmbh | Mass spectrometric mutation analysis with photolytically cleavable primers |
US6699670B2 (en) | 2001-03-01 | 2004-03-02 | The Johns Hopkins University | Quantitative assay for the simultaneous detection and speciation of bacterial infections |
US7718354B2 (en) | 2001-03-02 | 2010-05-18 | Ibis Biosciences, Inc. | Methods for rapid identification of pathogens in humans and animals |
US7226739B2 (en) * | 2001-03-02 | 2007-06-05 | Isis Pharmaceuticals, Inc | Methods for rapid detection and identification of bioagents in epidemiological and forensic investigations |
US20030027135A1 (en) | 2001-03-02 | 2003-02-06 | Ecker David J. | Method for rapid detection and identification of bioagents |
US7666588B2 (en) | 2001-03-02 | 2010-02-23 | Ibis Biosciences, Inc. | Methods for rapid forensic analysis of mitochondrial DNA and characterization of mitochondrial DNA heteroplasmy |
US20040121310A1 (en) | 2002-12-18 | 2004-06-24 | Ecker David J. | Methods for rapid detection and identification of bioagents in forensic studies |
AU2003298030B2 (en) | 2001-03-02 | 2009-12-10 | Ibis Biosciences, Inc. | Methods for rapid identification of pathogens in humans and animals |
US20040121313A1 (en) | 2002-12-06 | 2004-06-24 | Ecker David J. | Methods for rapid detection and identification of bioagents in organs for transplantation |
US20040121314A1 (en) | 2002-12-06 | 2004-06-24 | Ecker David J. | Methods for rapid detection and identification of bioagents in containers |
US20040038206A1 (en) * | 2001-03-14 | 2004-02-26 | Jia Zhang | Method for high throughput assay of genetic analysis |
US20030104410A1 (en) | 2001-03-16 | 2003-06-05 | Affymetrix, Inc. | Human microarray |
ATE335754T1 (en) | 2001-03-19 | 2006-09-15 | Harvard College | DEVELOPMENT OF NEW MOLECULAR FUNCTIONS |
JP4927309B2 (en) | 2001-03-28 | 2012-05-09 | カウンスィル オブ サイエンティフィック アンド インダストリアル リサーチ | Universal primers for wildlife identification |
US7630836B2 (en) | 2001-05-30 | 2009-12-08 | The Kitasato Institute | Polynucleotides |
CA2348042A1 (en) | 2001-06-04 | 2002-12-04 | Ann Huletsky | Sequences for detection and identification of methicillin-resistant staphylococcus aureus |
US20020187477A1 (en) * | 2001-06-06 | 2002-12-12 | Hong Xue | Method for detecting single nucleotide polymorphisms (SNPs) and point mutations |
ES2311613T3 (en) | 2001-06-06 | 2009-02-16 | Dsm Ip Assets B.V. | IMPROVED PRODUCTION OF ISOPRENOIDS. |
GB0113908D0 (en) | 2001-06-07 | 2001-08-01 | Univ London | Designing degenerate PCR primers |
US20020187490A1 (en) | 2001-06-07 | 2002-12-12 | Michigan State University | Microbial identification chip based on DNA-DNA hybridization |
GB0113907D0 (en) | 2001-06-07 | 2001-08-01 | Univ London | Virus detection using degenerate PCR primers |
US8073627B2 (en) | 2001-06-26 | 2011-12-06 | Ibis Biosciences, Inc. | System for indentification of pathogens |
US7217510B2 (en) | 2001-06-26 | 2007-05-15 | Isis Pharmaceuticals, Inc. | Methods for providing bacterial bioagent characterizing information |
DE10132147B4 (en) | 2001-07-03 | 2004-04-15 | Universität Leipzig | Method for the rapid quantitative determination of Eu bacteria |
GB0117054D0 (en) | 2001-07-12 | 2001-09-05 | Plant Bioscience Ltd | Methods and means for modification of plant characteristics |
EP1407051B1 (en) | 2001-07-19 | 2006-04-12 | Infectio Diagnostic (I.D.I.) INC. | Universal method and composition for the rapid lysis of cells for the release of nucleic acids and their detection |
US20040191769A1 (en) | 2001-07-24 | 2004-09-30 | Transgenomic, Inc. | Methods, compositions, and kits for mutation detection in mitochondrial DNA |
AU2002325197A1 (en) | 2001-07-30 | 2003-02-17 | Den Kgl. Veterinaer- Og Landbohojskole | Bacterial strains belonging to lactobacillus species and their use in food and feed industry |
US7115385B2 (en) | 2001-08-02 | 2006-10-03 | North Carolina State University | Media and methods for cultivation of microorganisms |
AT411174B (en) | 2001-08-09 | 2003-10-27 | Lambda Labor Fuer Molekularbio | METHOD AND CHIP FOR ANALYZING NUCLEIC ACIDS |
US20030039976A1 (en) * | 2001-08-14 | 2003-02-27 | Haff Lawrence A. | Methods for base counting |
US20040175715A1 (en) | 2001-08-21 | 2004-09-09 | Burgoyne Leigh A. | Method and device for simultaneously molecularly cloning and polylocus profiling of genomes or genomes mixtures |
US7105296B2 (en) | 2001-08-29 | 2006-09-12 | E. I. Du Pont De Nemours And Company | Genes encoding Baeyer-Villiger monooxygenases |
US7049286B2 (en) | 2001-08-30 | 2006-05-23 | Diatos, S.A. | Insulin conjugates and methods of use thereof |
US7060809B2 (en) | 2001-09-04 | 2006-06-13 | Exiqon A/S | LNA compositions and uses thereof |
US20040101809A1 (en) | 2001-09-21 | 2004-05-27 | Weiss Ervin I | Device, method and materials for mobilizing substances into dentinal tubules in root canal treatment |
DE10150121B4 (en) | 2001-10-11 | 2005-12-01 | Bernhard-Nocht-Institut für Tropenmedizin | Real-time detection of DNA amplification products |
US7297485B2 (en) | 2001-10-15 | 2007-11-20 | Qiagen Gmbh | Method for nucleic acid amplification that results in low amplification bias |
US6977148B2 (en) | 2001-10-15 | 2005-12-20 | Qiagen Gmbh | Multiple displacement amplification |
US20040029129A1 (en) * | 2001-10-25 | 2004-02-12 | Liangsu Wang | Identification of essential genes in microorganisms |
DE10152821B4 (en) | 2001-10-25 | 2006-11-16 | Bruker Daltonik Gmbh | Mass spectra without electronic noise |
EP1308506A1 (en) | 2001-11-06 | 2003-05-07 | Eidgenössische Technische Hochschule Zürich | Mixtures of Propionibacterium jensenii and Lactobacillus sp. with antimicrobial activities for use as a natural preservation system |
NZ532635A (en) | 2001-11-13 | 2007-05-31 | Univ Pennsylvania | A method of identifying unknown adeno-associated virus (AAV) sequences and a kit for the method |
BR0206488A (en) | 2001-11-15 | 2008-08-05 | Whatman Inc | device, method and kit for storing and analyzing a portion containing nucleic acid in a biological sample |
US20040023209A1 (en) * | 2001-11-28 | 2004-02-05 | Jon Jonasson | Method for identifying microorganisms based on sequencing gene fragments |
JP3692067B2 (en) | 2001-11-30 | 2005-09-07 | 株式会社東芝 | Polishing slurry for copper CMP and method of manufacturing semiconductor device using the same |
US20030148284A1 (en) | 2001-12-17 | 2003-08-07 | Vision Todd J. | Solid phase detection of nucleic acid molecules |
TW509116U (en) | 2001-12-18 | 2002-11-01 | Ind Tech Res Inst | Device for clipping and tightening spindle of honing and milling machine |
US20030175709A1 (en) | 2001-12-20 | 2003-09-18 | Murphy George L. | Method and system for depleting rRNA populations |
US7468185B2 (en) | 2001-12-21 | 2008-12-23 | Pfizer Inc. | Vaccine for periodontal disease |
AU2002358353A1 (en) | 2001-12-28 | 2003-07-30 | Keygene N.V. | Discrimination and detection of target nucleotide sequences using mass spectrometry |
EP1333101B1 (en) | 2002-02-01 | 2007-03-28 | Bruker Daltonik GmbH | Mutation analysis by PCR and Mass spectrometry |
KR100600988B1 (en) | 2002-03-13 | 2006-07-13 | 주식회사 엘지생명과학 | Method for enhancing immune responses by codelivering influenza NP DNA in DNA immunization |
US7024370B2 (en) | 2002-03-26 | 2006-04-04 | P) Cis, Inc. | Methods and apparatus for early detection of health-related events in a population |
US6897027B2 (en) | 2002-03-27 | 2005-05-24 | Decode Genetics Ehf. | Method for desalting nucleic acids |
WO2003100035A2 (en) | 2002-04-01 | 2003-12-04 | Isis Pharmaceuticals, Inc. | Method for rapid detection and identification of viral bioagents |
US7539579B2 (en) | 2002-04-09 | 2009-05-26 | Beattie Kenneth L | Oligonucleotide probes for genosensor chips |
FR2838740A1 (en) | 2002-04-22 | 2003-10-24 | Centre Nat Rech Scient | Detecting primate T cell lymphoma/leukemia viruses, useful e.g. for diagnosis and drug screening, using degenerate oligonucleotides based on conserved regions of envelope protein |
GB0209812D0 (en) | 2002-04-30 | 2002-06-05 | Renovo Ltd | Genetic testing |
DE10222632B4 (en) | 2002-05-17 | 2006-03-09 | Con Cipio Gmbh | Microsatellite markers for genetic analysis and for the differentiation of roses |
US6906319B2 (en) | 2002-05-17 | 2005-06-14 | Micromass Uk Limited | Mass spectrometer |
EP1365031A1 (en) | 2002-05-21 | 2003-11-26 | MTM Laboratories AG | Method for detection of somatic mutations using mass spectometry |
US20030220844A1 (en) | 2002-05-24 | 2003-11-27 | Marnellos Georgios E. | Method and system for purchasing genetic data |
US20040014957A1 (en) * | 2002-05-24 | 2004-01-22 | Anne Eldrup | Oligonucleotides having modified nucleoside units |
JP2005534292A (en) | 2002-05-29 | 2005-11-17 | アレサ・バイオディテクション・アンパルトセルスカブ | Reporter system for plants |
GB0212666D0 (en) | 2002-05-31 | 2002-07-10 | Secr Defence | Immunogenic sequences |
AU2003238930A1 (en) | 2002-06-07 | 2003-12-22 | Incyte Corporation | Enzymes |
WO2003106635A2 (en) | 2002-06-13 | 2003-12-24 | Regulome Corp | Functional sites |
KR100484144B1 (en) * | 2002-06-20 | 2005-04-18 | 삼성전자주식회사 | Remote management server and the method thereof |
CA2490283A1 (en) | 2002-07-01 | 2004-01-08 | Wayne State University | Methods and compositions for the identification of antibiotics that are not susceptible to antibiotic resistance |
WO2004009849A1 (en) | 2002-07-19 | 2004-01-29 | Isis Pharmaceuticals, Inc. | Methods for mass spectrometry analysis utilizing an integrated microfluidics sample platform |
US6916483B2 (en) * | 2002-07-22 | 2005-07-12 | Biodynamics, Llc | Bioabsorbable plugs containing drugs |
GB0217434D0 (en) | 2002-07-27 | 2002-09-04 | Royal Vetinary College | Biological material |
US20040022764A1 (en) * | 2002-07-31 | 2004-02-05 | Hanan Polansky | Inhibition of microcompetition with a foreign polynucleotide as treatment of chronic disease |
US7172868B2 (en) | 2002-08-01 | 2007-02-06 | The Regents Of The University Of California | Nucleotide sequences specific to Francisella tularensis and methods for the detection of Francisella tularensis |
US20040038385A1 (en) * | 2002-08-26 | 2004-02-26 | Langlois Richard G. | System for autonomous monitoring of bioagents |
CA2497988C (en) * | 2002-09-06 | 2011-03-29 | The Trustees Of Boston University | Quantification of gene expression |
CA2410795A1 (en) | 2002-11-01 | 2004-05-01 | University Of Ottawa | A method for the amplification of multiple genetic targets |
WO2004044247A2 (en) | 2002-11-12 | 2004-05-27 | Genolife | One step real-time rt pcr kits for the universal detection of organisms in industrial products |
US7250496B2 (en) | 2002-11-14 | 2007-07-31 | Rosetta Genomics Ltd. | Bioinformatically detectable group of novel regulatory genes and uses thereof |
WO2004070001A2 (en) | 2002-11-15 | 2004-08-19 | Gen-Probe Incorporated | Assay and compositions for detection of bacillus anthracis nucleic acid |
US6680476B1 (en) * | 2002-11-22 | 2004-01-20 | Agilent Technologies, Inc. | Summed time-of-flight mass spectrometry utilizing thresholding to reduce noise |
EP1578399A4 (en) | 2002-12-06 | 2007-11-28 | Isis Pharmaceuticals Inc | Methods for rapid identification of pathogens in humans and animals |
US20040121312A1 (en) | 2002-12-18 | 2004-06-24 | Ecker David J. | Methods for rapid detection and identification of the absence of bioagents |
US20040122857A1 (en) | 2002-12-18 | 2004-06-24 | Ecker David J. | Secondary structure defining database and methods for determining identity and geographic origin of an unknown bioagent in forensic studies thereby |
US20040121315A1 (en) | 2002-12-18 | 2004-06-24 | Ecker David J. | Secondary structure defining database and methods for determining identity and geographic origin of an unknown bioagent in containers thereby |
US20040121329A1 (en) | 2002-12-18 | 2004-06-24 | Ecker David J. | Secondary structure defining database and methods for determining identity and geographic origin of an unknown bioagent in blood, bodily fluids, and bodily tissues thereby |
US20040122598A1 (en) | 2002-12-18 | 2004-06-24 | Ecker David J. | Secondary structure defining database and methods for determining identity and geographic origin of an unknown bioagent in food products and cosmetics thereby |
US20040121340A1 (en) | 2002-12-18 | 2004-06-24 | Ecker David J. | Secondary structure defining database and methods for determining identity and geographic origin of an unknown bioagent associated with host versus graft and graft versus host rejections thereby |
US9487823B2 (en) | 2002-12-20 | 2016-11-08 | Qiagen Gmbh | Nucleic acid amplification |
US20040170954A1 (en) | 2003-02-10 | 2004-09-02 | Mckenney Keith | Pathogen inactivation assay |
US20040170981A1 (en) | 2003-02-10 | 2004-09-02 | Mckenney Keith | Real-time polymerase chain reaction using large target amplicons |
US20040185438A1 (en) | 2003-03-10 | 2004-09-23 | Ecker David J. | Methods of detection and notification of bioagent contamination |
US20050065813A1 (en) * | 2003-03-11 | 2005-03-24 | Mishelevich David J. | Online medical evaluation system |
US8046171B2 (en) | 2003-04-18 | 2011-10-25 | Ibis Biosciences, Inc. | Methods and apparatus for genetic evaluation |
US8057993B2 (en) | 2003-04-26 | 2011-11-15 | Ibis Biosciences, Inc. | Methods for identification of coronaviruses |
WO2005009202A2 (en) | 2003-05-12 | 2005-02-03 | Isis Pharmaceuticals, Inc. | Automatic identification of bioagents |
US7964343B2 (en) | 2003-05-13 | 2011-06-21 | Ibis Biosciences, Inc. | Method for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture |
US8158354B2 (en) | 2003-05-13 | 2012-04-17 | Ibis Biosciences, Inc. | Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture |
WO2005003384A1 (en) | 2003-07-03 | 2005-01-13 | Danmarks Og Grønlands Geologiske Undersøgelse | Method for selective detection of a target nucleic acid |
WO2005017173A1 (en) * | 2003-07-29 | 2005-02-24 | Sigma Aldrich Co. | Methods and compositions for amplification of dna |
JP4304292B2 (en) * | 2003-07-30 | 2009-07-29 | 日本電気株式会社 | Mobile communication system, mobile communication terminal, power control method used therefor, and program thereof |
KR100632429B1 (en) | 2003-08-01 | 2006-10-09 | 프로테온 주식회사 | Screening system of reassortant influenza viruses using primer dependent multiplex RT-PCR |
US20060240412A1 (en) | 2003-09-11 | 2006-10-26 | Hall Thomas A | Compositions for use in identification of adenoviruses |
US20050142584A1 (en) | 2003-10-01 | 2005-06-30 | Willson Richard C. | Microbial identification based on the overall composition of characteristic oligonucleotides |
WO2005036369A2 (en) | 2003-10-09 | 2005-04-21 | Isis Pharmaceuticals, Inc. | Database for microbial investigations |
FR2861743B1 (en) | 2003-11-04 | 2007-10-19 | Univ Aix Marseille Ii | MOLECULAR IDENTIFICATION OF BACTERIA OF THE GENUS CORYNEBACTERIUM |
JP3861871B2 (en) | 2003-11-26 | 2006-12-27 | サンケン電気株式会社 | Switching power supply |
US8163895B2 (en) | 2003-12-05 | 2012-04-24 | Ibis Biosciences, Inc. | Compositions for use in identification of orthopoxviruses |
US20050250125A1 (en) | 2003-12-19 | 2005-11-10 | Novakoff James L | Method for conducting pharmacogenomics-based studies |
ATE485401T1 (en) | 2004-02-10 | 2010-11-15 | Hoffmann La Roche | NEW PRIMERS AND PROBE FOR DETECTING PARVOVIRUS B19 |
US7666592B2 (en) * | 2004-02-18 | 2010-02-23 | Ibis Biosciences, Inc. | Methods for concurrent identification and quantification of an unknown bioagent |
CA2560521C (en) | 2004-02-18 | 2012-01-03 | Isis Pharmaceuticals, Inc. | Compositions for use in identification of bacteria |
US8119336B2 (en) | 2004-03-03 | 2012-02-21 | Ibis Biosciences, Inc. | Compositions for use in identification of alphaviruses |
US7312036B2 (en) * | 2004-03-22 | 2007-12-25 | Isis Pharmaceuticals, Inc. | Compositions for use in identification of viral hemorrhagic fever viruses |
US20050266411A1 (en) | 2004-05-25 | 2005-12-01 | Hofstadler Steven A | Methods for rapid forensic analysis of mitochondrial DNA |
US7627437B2 (en) | 2005-01-14 | 2009-12-01 | Idaho Research Foundation | Categorization of microbial communities |
DE102005008583B4 (en) | 2005-02-24 | 2007-10-25 | Johannes-Gutenberg-Universität Mainz | A method of typing an individual by short tandem repeat (STR) loci of the genomic DNA |
CA2600184A1 (en) | 2005-03-03 | 2006-09-08 | Isis Pharmaceuticals, Inc. | Compositions for use in identification of adventitious viruses |
DE602006017365D1 (en) | 2005-04-13 | 2010-11-18 | Ibis Biosciences Inc | COMPOSITIONS FOR THE IDENTIFICATION OF ADENOVERS |
CA2607468A1 (en) | 2005-04-21 | 2006-11-02 | Isis Pharmaceuticals, Inc. | Compositions for use in identification of bacteria |
AU2006272776B2 (en) | 2005-07-21 | 2012-01-19 | Ibis Biosciences, Inc. | Methods for rapid identification and quantitation of nucleic acid variants |
US20070026435A1 (en) | 2005-07-28 | 2007-02-01 | Polysciences, Inc. | Hydroxysilane functionalized magnetic particles and nucleic acid separation method |
EP2126132B1 (en) | 2007-02-23 | 2013-03-20 | Ibis Biosciences, Inc. | Methods for rapid foresnsic dna analysis |
US20100204266A1 (en) | 2007-03-23 | 2010-08-12 | Ibis Biosciences, INC | Compositions for use in identification of mixed populations of bioagents |
JP5067239B2 (en) | 2008-03-28 | 2012-11-07 | 富士通株式会社 | Cable management device and electronic device device |
-
2004
- 2004-05-12 US US10/844,938 patent/US7964343B2/en not_active Expired - Fee Related
- 2004-05-13 JP JP2006533082A patent/JP2007516425A/en active Pending
- 2004-05-13 KR KR1020057021495A patent/KR100779138B1/en not_active IP Right Cessation
- 2004-05-13 AU AU2004239340A patent/AU2004239340A1/en not_active Abandoned
- 2004-05-13 CA CA2525498A patent/CA2525498C/en not_active Expired - Fee Related
- 2004-05-13 WO PCT/US2004/015196 patent/WO2004101809A2/en active Application Filing
- 2004-05-13 EP EP04752257A patent/EP1623039B1/en not_active Expired - Lifetime
- 2004-05-13 CN CN2004800161879A patent/CN1806050B/en not_active Expired - Fee Related
-
2008
- 2008-08-14 AU AU2008205432A patent/AU2008205432B8/en not_active Ceased
-
2012
- 2012-01-26 JP JP2012013771A patent/JP2012132924A/en active Pending
Non-Patent Citations (1)
Title |
---|
See references of EP1623039A4 * |
Cited By (81)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8268565B2 (en) | 2001-03-02 | 2012-09-18 | Ibis Biosciences, Inc. | Methods for identifying bioagents |
US8017358B2 (en) | 2001-03-02 | 2011-09-13 | Ibis Biosciences, Inc. | Method for rapid detection and identification of bioagents |
US8815513B2 (en) | 2001-03-02 | 2014-08-26 | Ibis Biosciences, Inc. | Method for rapid detection and identification of bioagents in epidemiological and forensic investigations |
US8563250B2 (en) | 2001-03-02 | 2013-10-22 | Ibis Biosciences, Inc. | Methods for identifying bioagents |
US9416424B2 (en) | 2001-03-02 | 2016-08-16 | Ibis Biosciences, Inc. | Methods for rapid identification of pathogens in humans and animals |
US8214154B2 (en) | 2001-03-02 | 2012-07-03 | Ibis Biosciences, Inc. | Systems for rapid identification of pathogens in humans and animals |
US8265878B2 (en) | 2001-03-02 | 2012-09-11 | Ibis Bioscience, Inc. | Method for rapid detection and identification of bioagents |
US9752184B2 (en) | 2001-03-02 | 2017-09-05 | Ibis Biosciences, Inc. | Methods for rapid forensic analysis of mitochondrial DNA and characterization of mitochondrial DNA heteroplasmy |
US8802372B2 (en) | 2001-03-02 | 2014-08-12 | Ibis Biosciences, Inc. | Methods for rapid forensic analysis of mitochondrial DNA and characterization of mitochondrial DNA heteroplasmy |
US8921047B2 (en) | 2001-06-26 | 2014-12-30 | Ibis Biosciences, Inc. | Secondary structure defining database and methods for determining identity and geographic origin of an unknown bioagent thereby |
US8073627B2 (en) | 2001-06-26 | 2011-12-06 | Ibis Biosciences, Inc. | System for indentification of pathogens |
US8380442B2 (en) | 2001-06-26 | 2013-02-19 | Ibis Bioscience, Inc. | Secondary structure defining database and methods for determining identity and geographic origin of an unknown bioagent thereby |
US8298760B2 (en) | 2001-06-26 | 2012-10-30 | Ibis Bioscience, Inc. | Secondary structure defining database and methods for determining identity and geographic origin of an unknown bioagent thereby |
US9725771B2 (en) | 2002-12-06 | 2017-08-08 | Ibis Biosciences, Inc. | Methods for rapid identification of pathogens in humans and animals |
US8822156B2 (en) | 2002-12-06 | 2014-09-02 | Ibis Biosciences, Inc. | Methods for rapid identification of pathogens in humans and animals |
US8057993B2 (en) | 2003-04-26 | 2011-11-15 | Ibis Biosciences, Inc. | Methods for identification of coronaviruses |
US7964343B2 (en) | 2003-05-13 | 2011-06-21 | Ibis Biosciences, Inc. | Method for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture |
US8476415B2 (en) | 2003-05-13 | 2013-07-02 | Ibis Biosciences, Inc. | Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture |
US8158354B2 (en) | 2003-05-13 | 2012-04-17 | Ibis Biosciences, Inc. | Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture |
US8013142B2 (en) | 2003-09-11 | 2011-09-06 | Ibis Biosciences, Inc. | Compositions for use in identification of bacteria |
US8546082B2 (en) | 2003-09-11 | 2013-10-01 | Ibis Biosciences, Inc. | Methods for identification of sepsis-causing bacteria |
US8097416B2 (en) | 2003-09-11 | 2012-01-17 | Ibis Biosciences, Inc. | Methods for identification of sepsis-causing bacteria |
US7956175B2 (en) | 2003-09-11 | 2011-06-07 | Ibis Biosciences, Inc. | Compositions for use in identification of bacteria |
US8163895B2 (en) | 2003-12-05 | 2012-04-24 | Ibis Biosciences, Inc. | Compositions for use in identification of orthopoxviruses |
US8187814B2 (en) | 2004-02-18 | 2012-05-29 | Ibis Biosciences, Inc. | Methods for concurrent identification and quantification of an unknown bioagent |
US9447462B2 (en) | 2004-02-18 | 2016-09-20 | Ibis Biosciences, Inc. | Methods for concurrent identification and quantification of an unknown bioagent |
US9449802B2 (en) | 2004-05-24 | 2016-09-20 | Ibis Biosciences, Inc. | Mass spectrometry with selective ion filtration by digital thresholding |
US8173957B2 (en) | 2004-05-24 | 2012-05-08 | Ibis Biosciences, Inc. | Mass spectrometry with selective ion filtration by digital thresholding |
US8987660B2 (en) | 2004-05-24 | 2015-03-24 | Ibis Biosciences, Inc. | Mass spectrometry with selective ion filtration by digital thresholding |
US8407010B2 (en) | 2004-05-25 | 2013-03-26 | Ibis Biosciences, Inc. | Methods for rapid forensic analysis of mitochondrial DNA |
US9873906B2 (en) | 2004-07-14 | 2018-01-23 | Ibis Biosciences, Inc. | Methods for repairing degraded DNA |
WO2006034294A1 (en) * | 2004-09-17 | 2006-03-30 | Isis Pharmaceuticals, Inc. | Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture |
US8084207B2 (en) | 2005-03-03 | 2011-12-27 | Ibis Bioscience, Inc. | Compositions for use in identification of papillomavirus |
US8182992B2 (en) | 2005-03-03 | 2012-05-22 | Ibis Biosciences, Inc. | Compositions for use in identification of adventitious viruses |
JP2008538505A (en) * | 2005-04-21 | 2008-10-30 | アイシス ファーマシューティカルズ インコーポレイティッド | Composition for use in bacterial identification |
US8116983B2 (en) | 2005-06-30 | 2012-02-14 | Biocrates Life Sciences Ag | Device for quantitative analysis of a drug or metabolite profile |
US8265877B2 (en) | 2005-06-30 | 2012-09-11 | Biocrates Life Sciences Ag | Apparatus and method for analyzing a metabolite profile |
JP2008547031A (en) * | 2005-06-30 | 2008-12-25 | バイオクラテス ライフ サイエンシズ アクチェンゲゼルシャフト | Equipment for quantitative analysis of metabolite characteristics |
US8551738B2 (en) | 2005-07-21 | 2013-10-08 | Ibis Biosciences, Inc. | Systems and methods for rapid identification of nucleic acid variants |
US8026084B2 (en) | 2005-07-21 | 2011-09-27 | Ibis Biosciences, Inc. | Methods for rapid identification and quantitation of nucleic acid variants |
WO2007066227A2 (en) * | 2005-12-05 | 2007-06-14 | Novartis Vaccines And Diagnostics Srl | Liquid chromatography-mass spectrometry analysis of samples using an ionic eluent comprising a volatile ionic salt |
WO2007066227A3 (en) * | 2005-12-05 | 2007-10-04 | Novartis Vaccines & Diagnostic | Liquid chromatography-mass spectrometry analysis of samples using an ionic eluent comprising a volatile ionic salt |
US9149473B2 (en) | 2006-09-14 | 2015-10-06 | Ibis Biosciences, Inc. | Targeted whole genome amplification method for identification of pathogens |
US7655792B2 (en) | 2006-09-26 | 2010-02-02 | Ge Healthcare Bio-Sciences Corp. | Nucleic acid purification method |
US7655794B2 (en) | 2006-09-26 | 2010-02-02 | Ge Healthcare Bio-Sciences Corp. | Nucleic acid purification method |
US7655793B2 (en) | 2006-09-26 | 2010-02-02 | Ge Healthcare Bio-Sciences Corp. | Nucleic acid purification method |
GB2445441B (en) * | 2006-09-26 | 2010-06-30 | Ge Healthcare Bio Sciences | Nucleic acid purification method |
GB2445442A (en) * | 2006-09-26 | 2008-07-09 | Ge Healthcare Bio Sciences | Nucleic acid purification using anion exchange |
US8871471B2 (en) | 2007-02-23 | 2014-10-28 | Ibis Biosciences, Inc. | Methods for rapid forensic DNA analysis |
US9598724B2 (en) | 2007-06-01 | 2017-03-21 | Ibis Biosciences, Inc. | Methods and compositions for multiple displacement amplification of nucleic acids |
US8534447B2 (en) | 2008-09-16 | 2013-09-17 | Ibis Biosciences, Inc. | Microplate handling systems and related computer program products and methods |
US9027730B2 (en) | 2008-09-16 | 2015-05-12 | Ibis Biosciences, Inc. | Microplate handling systems and related computer program products and methods |
US8252599B2 (en) | 2008-09-16 | 2012-08-28 | Ibis Biosciences, Inc. | Sample processing units, systems, and related methods |
US8148163B2 (en) | 2008-09-16 | 2012-04-03 | Ibis Biosciences, Inc. | Sample processing units, systems, and related methods |
US8550694B2 (en) | 2008-09-16 | 2013-10-08 | Ibis Biosciences, Inc. | Mixing cartridges, mixing stations, and related kits, systems, and methods |
US8609430B2 (en) | 2008-09-16 | 2013-12-17 | Ibis Biosciences, Inc. | Sample processing units, systems, and related methods |
US9023655B2 (en) | 2008-09-16 | 2015-05-05 | Ibis Biosciences, Inc. | Sample processing units, systems, and related methods |
US8796617B2 (en) | 2009-02-12 | 2014-08-05 | Ibis Biosciences, Inc. | Ionization probe assemblies |
US9165740B2 (en) | 2009-02-12 | 2015-10-20 | Ibis Biosciences, Inc. | Ionization probe assemblies |
US8158936B2 (en) | 2009-02-12 | 2012-04-17 | Ibis Biosciences, Inc. | Ionization probe assemblies |
US9393564B2 (en) | 2009-03-30 | 2016-07-19 | Ibis Biosciences, Inc. | Bioagent detection systems, devices, and methods |
US9194877B2 (en) | 2009-07-17 | 2015-11-24 | Ibis Biosciences, Inc. | Systems for bioagent indentification |
US8950604B2 (en) | 2009-07-17 | 2015-02-10 | Ibis Biosciences, Inc. | Lift and mount apparatus |
US9416409B2 (en) | 2009-07-31 | 2016-08-16 | Ibis Biosciences, Inc. | Capture primers and capture sequence linked solid supports for molecular diagnostic tests |
US10119164B2 (en) | 2009-07-31 | 2018-11-06 | Ibis Biosciences, Inc. | Capture primers and capture sequence linked solid supports for molecular diagnostic tests |
WO2011047307A1 (en) | 2009-10-15 | 2011-04-21 | Ibis Biosciences, Inc. | Multiple displacement amplification |
EP3225695A1 (en) | 2009-10-15 | 2017-10-04 | Ibis Biosciences, Inc. | Multiple displacement amplification |
EP2957641A1 (en) | 2009-10-15 | 2015-12-23 | Ibis Biosciences, Inc. | Multiple displacement amplification |
US9890408B2 (en) | 2009-10-15 | 2018-02-13 | Ibis Biosciences, Inc. | Multiple displacement amplification |
WO2011112718A1 (en) | 2010-03-10 | 2011-09-15 | Ibis Biosciences, Inc. | Production of single-stranded circular nucleic acid |
US9752173B2 (en) | 2010-04-08 | 2017-09-05 | Ibis Biosciences, Inc. | Compositions and methods for inhibiting terminal transferase activity |
US9068017B2 (en) | 2010-04-08 | 2015-06-30 | Ibis Biosciences, Inc. | Compositions and methods for inhibiting terminal transferase activity |
EP3170831A1 (en) | 2011-09-06 | 2017-05-24 | Ibis Biosciences, Inc. | Sample preparation methods |
WO2013036603A1 (en) | 2011-09-06 | 2013-03-14 | Ibis Biosciences, Inc. | Sample preparation methods |
US9970061B2 (en) | 2011-12-27 | 2018-05-15 | Ibis Biosciences, Inc. | Bioagent detection oligonucleotides |
US10662485B2 (en) | 2011-12-27 | 2020-05-26 | Ibis Biosciences, Inc. | Bioagent detection oligonucleotides |
US9371524B2 (en) | 2012-04-05 | 2016-06-21 | Roche Molecular Systems, Inc. | Amine compounds for the selective preparation of biological samples |
EP2647709A1 (en) * | 2012-04-05 | 2013-10-09 | F. Hoffmann-La Roche AG | Amine compounds for the selective preparation of biological samples |
WO2014032990A1 (en) * | 2012-08-30 | 2014-03-06 | Qiagen Gmbh | Method of determining the presence or absence of a target nucleic acid in a cell sample |
CN111690719A (en) * | 2012-08-30 | 2020-09-22 | 凯杰有限公司 | Method for determining the presence or absence of a target nucleic acid in a cell sample |
WO2014052590A1 (en) | 2012-09-26 | 2014-04-03 | Ibis Biosciences, Inc. | Swab interface for a microfluidic device |
Also Published As
Publication number | Publication date |
---|---|
CN1806050A (en) | 2006-07-19 |
AU2004239340A1 (en) | 2004-11-25 |
EP1623039A4 (en) | 2006-04-12 |
AU2008205432B8 (en) | 2012-01-19 |
KR100779138B1 (en) | 2007-11-28 |
JP2012132924A (en) | 2012-07-12 |
JP2007516425A (en) | 2007-06-21 |
CA2525498A1 (en) | 2004-11-25 |
US7964343B2 (en) | 2011-06-21 |
AU2008205432B2 (en) | 2011-08-11 |
US20050164215A1 (en) | 2005-07-28 |
AU2008205432A1 (en) | 2008-09-04 |
EP1623039B1 (en) | 2012-08-01 |
WO2004101809A3 (en) | 2005-09-01 |
CA2525498C (en) | 2010-12-07 |
KR20060057533A (en) | 2006-05-26 |
EP1623039A2 (en) | 2006-02-08 |
CN1806050B (en) | 2011-11-02 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
EP1623039B1 (en) | Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture | |
US8476415B2 (en) | Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture | |
US20160376581A1 (en) | Method for the purification of targeted nucleic acids from background nucleic acids | |
US20140094597A1 (en) | Method for isolating nucleic acids comprising the use of ethylene glycol multimers | |
EP1240355B1 (en) | Removal of dye-labeled dideoxy terminators from dna sequencing reactions | |
JP2006311803A (en) | Method for purifying nucleic acid and tool for purifying nucleic acid | |
US20180257002A1 (en) | Methods for obtaining liquid from a solid phase | |
US20140011201A1 (en) | Method for the purification of targeted nucleic acids from background nucleic acids | |
US8586350B2 (en) | Mechanism of separating and purifying DNA and the like | |
WO2015188994A1 (en) | Method for the enrichment and/or purification of nucleic acids | |
US10457931B2 (en) | Purification of nucleic acid from a sample containing nucleic acid and endotoxin | |
US20070073049A1 (en) | Method and apparatus for nucleic purification using ion-permeable polymer-coated electrode | |
EP4253538A1 (en) | Method for isolating nucleic acid using binding buffer including compound having low or intermediate dielectric constant | |
CN114921458A (en) | Nucleic acid extraction and purification system and method for extracting and purifying nucleic acid by using same |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AK | Designated states |
Kind code of ref document: A2 Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BW BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE EG ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NA NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW |
|
AL | Designated countries for regional patents |
Kind code of ref document: A2 Designated state(s): BW GH GM KE LS MW MZ NA SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG |
|
121 | Ep: the epo has been informed by wipo that ep was designated in this application | ||
WWE | Wipo information: entry into national phase |
Ref document number: 2525498 Country of ref document: CA |
|
WWE | Wipo information: entry into national phase |
Ref document number: 1020057021495 Country of ref document: KR |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2006533082 Country of ref document: JP |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2004239340 Country of ref document: AU |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2004752257 Country of ref document: EP |
|
WWE | Wipo information: entry into national phase |
Ref document number: 20048161879 Country of ref document: CN |
|
ENP | Entry into the national phase |
Ref document number: 2004239340 Country of ref document: AU Date of ref document: 20040513 Kind code of ref document: A |
|
WWP | Wipo information: published in national office |
Ref document number: 2004239340 Country of ref document: AU |
|
WWP | Wipo information: published in national office |
Ref document number: 2004752257 Country of ref document: EP |
|
WWP | Wipo information: published in national office |
Ref document number: 1020057021495 Country of ref document: KR |