WO2004087185A1 - Process for preparing antibacterial and antioxidant fraction from seabuckthorn seeds (hippophae rhamnoides l.) - Google Patents
Process for preparing antibacterial and antioxidant fraction from seabuckthorn seeds (hippophae rhamnoides l.) Download PDFInfo
- Publication number
- WO2004087185A1 WO2004087185A1 PCT/IN2003/000105 IN0300105W WO2004087185A1 WO 2004087185 A1 WO2004087185 A1 WO 2004087185A1 IN 0300105 W IN0300105 W IN 0300105W WO 2004087185 A1 WO2004087185 A1 WO 2004087185A1
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- extract
- seabuckthom
- seeds
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- antioxidant
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
Definitions
- the present invention relates to a one step process for the preparation of antibacterial and antioxidant fraction from the unconventional parts of Seabuckthom (Hippophae rhamnoides L.). Background of the invention
- Seabuckthom berries have been reported to posses antibacterial, antioxidant, anti-inflammatory, antiallergenic and analgesic activity [Benavente-Garcia O, Castillo J, Martin FR, Ortuno A, Del Rio JA. Journal of Agriculture and Food Chemistry 1997; 45: 4505].
- Seabuckthom is widely grown plant which belongs to Elaeagnaceae family and genus Hippophae.. It has been used in traditional Vietnamese and Maiigolian medicines (Lu Rongsen, 1992. Seabuckthom: A multiple plant species for fragile mountains. International center for integrated mountain development, ICIMOD occasional paper No. 20, Kathmandu, Nepal).
- Seabuckthom is known as wonder plant that bears small orange yellow to red colored fruits on two year old thorny twigs.
- the berry like fruit develops from an ovary or calyx tube, connected to ovary.
- Berries contain many bioactive substances and can be used in the treatment of several diseases like cardiovascular disease, cancer, acute mountain sickness etc.
- a total of more than 300 different medicine preparations involving seabuckthom have been reported (Singh, 2001. International workshop on seabuckthom, during 18-21 Feb 2001, New Delhi).
- the main object of the present invention is to provide a one step process for preparation of a bioactive fraction, which has antibacterial and antioxidant activities.
- a process for the preparation of bioactive fraction from the unconventional parts of Seabuckthom (Hippophae rhamnoides L.), to which no commercial value has been attributed so far.
- Another object of the present invention is to provide a technology for the preparation of an antibacterial and antioxidant fraction from the unconventional parts of Seabuckthom (Hippophae rhamnoides L.), which can be used as potential natural preservative.
- Yet another object of the present invention is to provide an efficient process for the large-scale preparation of antibacterial and antioxidant fraction from the unconventional parts of Seabuckthom (Hippophae rhamnoides L.).
- Still another object of the present invention is to provide a one step process for the preparation of antibacterial and antioxidant fraction from the unconventional parts of Seabuckthom (Hippophae rhamnoides L.).
- the present invention provides the one step process for the preparation of antibacterial and antioxidant fraction, which comprises washing the seeds of seabuckthom (Hippophae rhamnoides L.) with tap water in order to remove any dirt or extraneous matter followed by drying at 40-50°C for a residence period of 3-4 hours; powdering the seeds of Seabuckthom (Hippophae rhamnoides L.) to get a particle size of 60-80 mesh; extracting of the above said material with leotropic series solvents (Elution effect in increasing order) followed by methanol in a Soxhlet extractor at a temperature of 55-60 °C for a period of 8-9 hours; filtering the above extract using Whatman filter paper no.41 to obtain the particle free extract; distilling the above extract in distillation unit at 60-65 °C to recover the solvent up to 80-90%, which is further recycled for further extraction in soxhlet unit; concentrating the above particle free extract under vacuum by employing 200-250 mbar pressure in flash evaporator at a temperature
- the dried product thus obtained had antibacterial activity in terms of minimum inhibitory concentration ( ⁇ g/ml) against different Gram positive and Gram negative bacteria in the range of 200-350 and antioxidant activity in terms of % radical scavenging activity in the range of 40.379-93.473 at 10-50 ⁇ g/ml concentration respectively as determined by DPPH method.
- the rotation of the flask containing methanol extract of seabuckthom seeds is used for concentration in flash evaporator at 80- 100 rpm under vacuum of 200-250 mbar by employing 60-65°C temperature.
- the yield of methanol extract was found to be 14.264 g/lOOg after freeze-drying and 14.4858 g/lOOg after vacuum drying.
- the process of the present invention involves following steps, which comprises of: i) Washing the seeds of seabuckthom (Hippophae rhamnoides L.) with tap water in order to remove any dirt or extraneous matter followed by drying at 40-50°C for a residence period of 3-4 hours, ii) Powdering the seeds of Seabuckthom (Hippophae rhamnoides L.) to get a particle size of 60-80 mesh.
- the dried product thus obtained had antibacterial activity in terms of minimum inhibitory concentration ( ⁇ g/ml) against different Gram positive and Gram negative bacteria in the range of 200-350 and antioxidant activity in terms of % radical scavenging activity in the range of 40.379-93.473 by using 10-50 ⁇ g/ml concentration respectively as determined by DPPH method.
- Example 1 lOOg seeds of Seabuckthom (Hippophae rhamnoides L.) were washed and dried in vacuum oven (pressure 175) at 40°C for 4 hours followed by powdering in mixer grinder at 18000 rpm to get a 60 mesh size powder particle.
- the powder was extracted using 200 ml of methanol at 60 °C for 8 h in a Soxhlet extractor.
- the methanol extract was filtered using Whatman filter paper No.41 in order to get clear extract and it was distilled at 60°C by using distillation unit to recover the 160 ml of solvent.
- the crude extract was concentrated under vacuum of 200 mbar pressure and flask rotation speed of 100 rpm was maintained in flash evaporator for a period of 2 hours and it was dried using freeze drying technique for the residency period of 6 hours for antioxidant activity determination and dried under vacuum for antibacterial activity at 175 mbar pressure for the residency period of 9 hours in a vacuum oven at 40 °C.
- the yield of the freeze-dried methanol extract for antioxidant activity and vacuum oven dried methanol extract for antibacterial assay were 14.264 g and 14.4858 g, respectively.
- Free radical-scavenging activity of each antioxidant was assayed using a stable free radical, DPPH (l,l-Diphenyl-2-picrylhydrazyl), according to the method of Blois (Blois, 1958.
- the reaction mixture contained 0.5 ml of 0.5mM DPPH and 0.1ml of dimethyl sulphoxide containing the antioxidant extract at different concentrations (10-50 ⁇ g). Finally, the total volume of the reaction mixture was adjusted to 1.0 ml by adding lOOmM Tris-HCl buffer (pH7.4). After the reaction was carried out at room temperature for 20 minutes in the dark, the radical-scavenging activity of each antioxidant was quantified by decolorisation at 517nm. The % radical scavenging activity on DPPH for the 10 ⁇ g/ml was 40.379.
- the antibacterial assay for the extract of Seabuckthom was tested by pour plate method against Bacillus cereus by the method of Negi et al. (J. Agricultural and Food Chemistry 47, 4297-4300, 1999).
- One hundred ⁇ (about 10 ⁇ cfu/ml) of culture was inoculated into the flasks under aseptic conditions. The media was then poured into sterilized petri plates in quadruplet and incubated at 37 °C for 24 h for growth.
- the minimum inhibitory concentration (MIC) was reported as the lowest concentration of the compound capable of inhibiting the complete growth of the bacterium being tested.
- the MIC value of Seabuckthom (Hippophae rhamnoides L.) seed extract against Bacillus cereus was 200 ppm.
- Example -2 lOOg seeds of Seabuckthom (Hippophae rhamnoides L.) were washed and dried in vacuum oven (pressure 180 mbar) at 40°C for 3 hours followed by powdering in mixer grinder at 18000 rpm to get a 70 mesh size powder particle. The powder was extracted using 200 ml of methanol at 55 °C for 9 h in a Soxhlet extractor.
- the methanol extract was filtered using Whatman filter paper No.41 in order to get clear extract and it was distilled at 65°C by using distillation unit to recover the 170 ml of solvent.
- the crude extract was concentrated under vacuum of 250 mbar pressure and flask rotation speed of 100 rpm was maintained in flash evaporator for a period of 2 hours and it was dried using freeze drying technique for the residency period of 6 hours and also dried under vacuum of 200 mbar for the residency period of 8 hours in a vacuum oven at 50 °C.
- the yield of the freeze-dried methanol extract for antioxidant activity and vacuum oven dried methanol extract for antibacterial assay were 14.264 g and 14.4858 g, respectively.
- Free radical-scavenging activity of each antioxidant was assayed using a stable free radical, DPPH (l,l-Diphenyl-2-picrylhydrazyl), according to the method of Blois (Blois, 1958. Antioxidant determinations by the use of a stable free radical . Nature, 181: 1199-1200).
- the reaction mixture contained 0.5 ml of 0.5mM DPPH and 0.1ml of dimethyl sulphoxide containing the antioxidant extract at different concentrations (10-50 ⁇ g). Finally, the total volume of the reaction mixture was adjusted to 1.0 ml by adding lOOmM Tris-HCl buffer (pH7.4).
- Example -3 lOOg seeds of Seabuckthom (Hippophae rhamnoides L.) were washed and dried in vacuum oven (pressure 200 mbar) at 45°C for 3 hours followed by powdering in mixer grinder at 18000 rpm to get a 80 mesh size powder particle.
- the powder was extracted using 200 ml of methanol at 60 °C for 8 h in a Soxhlet extractor.
- the methanol extract was filtered using Whatman filter paper No.41 in order to get clear extract and it was distilled at 60°C by using distillation unit to recover the 175 ml of solvent.
- the cmde extract was concentrated under vacuum of 200-250 mbar pressure and flask rotation speed of 100 rpm was maintained in flash evaporator for a period of 2 hours and it was dried using freeze drying technique for the residency period of 6 hours and also dried under vacuum of 175-200 mbar for the residency period of 8 hours in a vacuum oven at 40 °C.
- the yield of the freeze-dried methanol extract for antioxidant activity and vacuum oven dried methanol extract for antibacterial assay were 14.264 g and 14.4858 g, respectively.
- Free radical-scavenging activity of each antioxidant was assayed using a stable free radical, DPPH (l,l-Diphenyl-2- picrylhydrazyl), according to the method of Blois (Blois, 1958.
- the reaction mixture contained 0.5 ml of 0.5mM DPPH and 0.1ml of dimethyl sulphoxide containing the antioxidant extract at different concentrations (10-50 ⁇ g). Finally, the total volume of the reaction mixture was adjusted to 1.0 ml by adding lOOmM Tris-HCl buffer (pH7.4). After the reaction was carried out at room temperature for 20 minutes in the dark, the radical-scavenging activity of each antioxidant was quantified by decolorisation at 517nm. The % radical scavenging activity on DPPH for the 30 ⁇ g/ml was 85.1777.
- the antibacterial assay for the extract of Seabuckthom was tested by pour plate method against Bacillus cereus by the method of Negi et al. (J. Agricultural and Food Chemistry 47, 4297-4300, 1999).
- One hundred ⁇ (about 10 ⁇ cfu/ml) of culture was inoculated into the flasks under aseptic conditions. The media was then poured into sterilized petri plates in quadruplet and incubated at 37 °C for 22 h for growth.
- the minimum inhibitory concentration (MIC) was reported as the lowest concentration of the compound capable of inhibiting the complete growth of the bacterium being tested.
- the MIC value of Seabuckthom (Hippophae rhamnoides L.) seed extract against Bacillus coagulans was 300 ppm.
- Example 4 lOOg seeds of Seabuckthom (Hippophae rhamnoides L.) were washed and dried in vacuum oven (pressure 200 mbar) at 50°C for 3 hours followed by powdering powdered using mixer grinder at 18000 rpm to get a 80 mesh size powder particle.
- the powder was extracted using 200 ml of methanol at 60 °C for 8 h in a Soxhlet extractor.
- the methanol extract was filtered using Whatman filter paper No.41 in order to get clear extract and it was distilled at 60°C by using distillation unit to recover the 175 ml of solvent.
- the crude extract was concentrated under vacuum of 250 mbar pressure and flask rotation speed of 100 rpm was maintained in flash evaporator for a period of 2 hours and it was dried using freeze drying technique for the residency period of 6 hours and also dried under vacuum of 200 mbar for the residency period of 8 hours in a vacuum oven at 40 °C.
- the yield of the freeze- dried methanol extract for antioxidant activity and vacuum oven dried methanol extract for antibacterial assay were 14.264 g and 14.4858 g, respectively.
- Free radical-scavenging activity of each antioxidant was assayed using a stable free radical, DPPH (l,l-Diphenyl-2- picrylhydrazyl), according to the method of Blois (Blois, 1958.
- the reaction mixture contained 0.5 ml of 0.5mM DPPH and 0.1ml of dimethyl sulphoxide containing the antioxidant extract at different concentrations (10-50 ⁇ g). Finally, the total volume of the reaction mixture was adjusted to 1.0 ml by adding lOOmM Tris-HCl buffer (pH7.4). After the reaction was carried out at room temperature for 20 minutes in the dark, the radical-scavenging activity of each antioxidant was quantified by decolorisation at 517nm. The % radical scavenging activity on DPPH for the 40 ⁇ g/ml was 92.2185.
- the antibacterial assay for the extract of Seabuckthom was tested by pour plate method against Bacillus cereus by the method of Negi et al. (J. Agricultural and Food Chemistry 47, 4297-4300, 1999).
- One hundred ⁇ (about 10 ⁇ cfu/ml) of culture was inoculated into the flasks under aseptic conditions. The media was then poured into sterilized petri plates in quadruplet and incubated at 37 °C for 20 h for growth.
- the minimum inhibitory concentration (MIC) was reported as the lowest concentration of the compound capable of inhibiting the complete growth of the bacterium being tested.
- the MIC value of Seabuckthom (Hippophae rhamnoides L.) seed extract against Pseudomonas aeruginosa was 300 ppm.
- Example 5 lOOg seeds of Seabuckthom (Hippophae rhamnoides L.) were washed and dried in vacuum oven (pressure 200 mbar) at 50°C for 3 hours followed by powdering in mixer grinder at 18000 rpm to get a 60 mesh size powder particle.
- the powder was extracted using 200 ml of methanol at 60 °C for 8 h in a Soxhlet extractor.
- the methanol extract was filtered using Whatman filter paper No.41 in order to get clear extract and it was distilled at 65 °C by using distillation unit to recover the 180 ml of solvent.
- the cmde extract was concentrated under vacuum of 250 mbar pressure and flask rotation speed of 100 rpm was maintained in flash evaporator for a period of 2 hours and it was dried using freeze drying technique for the residency period of 6 hours and also dried under vacuum of 200 mbar for the residency period of 8 hours in a vacuum oven at 50 °C.
- the yield of the freeze-dried methanol extract for antioxidant activity and vacuum oven dried methanol extract for antibacterial assay were 14.264 g and 14.4858 g, respectively.
- Free radical-scavenging activity of each antioxidant was assayed using a stable free radical, DPPH (l,l-Diphenyl-2-picrylhydrazyl), according to the method of Blois (Blois, 1958.
- the reaction mixture contained 0.5 ml of 0.5mM DPPH and 0.1ml of dimethyl sulphoxide containing the antioxidant extract at different concentrations (10-50 ⁇ g). Finally, the total volume of the reaction mixture was adjusted to 1.0 ml by adding lOOmM Tris-HCl buffer (pH7.4). After the reaction was carried out at room temperature for 20 minutes in the dark, the radical-scavenging activity of each antioxidant was quantified by decolorisation at 517nm. The % radical scavenging activity on DPPH for the 50 ⁇ g/ml was 93.473.
- the antibacterial assay for the extract of Seabuckthom was tested by pour plate method against Bacillus cereus by the method of Negi et al. (J. Agricultural and Food Chemistry 47, 4297-4300, 1999).
- One hundred ⁇ (about l ⁇ 3 efu/ml) of culture was inoculated into the flasks under aseptic conditions. The media was then poured into sterilized petri plates in quadruplet and incubated at 37 °C for 20 h for growth.
- the minimum inhibitory concentration (MIC) was reported as the lowest concentration of the compound capable of inhibiting the complete growth of the bacterium being tested.
- the MIC value of Seabuckthom (Hippophae rhamnoides L.) seed extract against Lister ia monocytogenes was 300 ppm.
- Example 6 lOOg seeds of Seabuckthom (Hippophae rhamnoides L.) were washed and dried in vacuum oven (pressure 200 mbar) at 40°C for 3 hours followed by powdering powdered using mixer grinder at 18000 rpm to get a 60 mesh size powder particle.
- the powder was extracted using 200 ml of methanol at 60 °C for 8 h in a Soxhlet extractor.
- the methanol extract was filtered using Whatman filter paper No.41 in order to get clear extract and it was distilled at 60°C by using distillation unit to recover the 180 ml of solvent.
- the crude extract was concentrated under vacuum of 200 mbar pressure and flask rotation speed of 100 rpm was maintained in flash evaporator for a period of 2 hours and it was dried using freeze drying technique for the residency period of 6 hours and also dried under vacuum of 175 mbar for the residency period of 9 hours in a vacuum oven at 40 °C.
- the yield of the freeze- dried methanol extract for antioxidant activity and vacuum oven dried methanol extract for antibacterial assay were 14.264 g and 14.4858 g, respectively.
- the antibacterial assay for the extract of Seabuckthom (Hippophae rhamnoides L.) was tested by pour plate method against Bacillus cereus by the method of Negi et al. (J. Agricultural and Food Chemistry 47, 4297- 4300, 1999).
- the raw material (seed) has no commercial value at present.
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Priority Applications (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN038263602A CN1767843B (en) | 2003-03-31 | 2003-03-31 | Method for preparing antimicrobial agent and antioxidant position from sea-buckthorn seeds |
PCT/IN2003/000105 WO2004087185A1 (en) | 2003-03-31 | 2003-03-31 | Process for preparing antibacterial and antioxidant fraction from seabuckthorn seeds (hippophae rhamnoides l.) |
AU2003226634A AU2003226634A1 (en) | 2003-03-31 | 2003-03-31 | Process for preparing antibacterial and antioxidant fraction from seabuckthorn seeds (hippophae rhamnoides l.) |
DE10394212T DE10394212T5 (en) | 2003-03-31 | 2003-03-31 | Process for the preparation of an antibacterial and antioxidant fraction from sea buckthorn seeds (Hippophae rhamnoides L.) |
FI20050980A FI20050980A (en) | 2003-03-31 | 2005-09-30 | Process for producing antibacterial and antioxidant fractions from oatmeal seeds |
Applications Claiming Priority (1)
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---|---|---|---|
PCT/IN2003/000105 WO2004087185A1 (en) | 2003-03-31 | 2003-03-31 | Process for preparing antibacterial and antioxidant fraction from seabuckthorn seeds (hippophae rhamnoides l.) |
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WO2004087185A1 true WO2004087185A1 (en) | 2004-10-14 |
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PCT/IN2003/000105 WO2004087185A1 (en) | 2003-03-31 | 2003-03-31 | Process for preparing antibacterial and antioxidant fraction from seabuckthorn seeds (hippophae rhamnoides l.) |
Country Status (5)
Country | Link |
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CN (1) | CN1767843B (en) |
AU (1) | AU2003226634A1 (en) |
DE (1) | DE10394212T5 (en) |
FI (1) | FI20050980A (en) |
WO (1) | WO2004087185A1 (en) |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2168587A1 (en) * | 2007-06-13 | 2010-03-31 | Qinghai Tsinghua Biotry Bio-tech Co., Ltd | A method for preparing a mixture containing natural vitamin p from hippophae rhamnoides l. |
ITSS20090006A1 (en) * | 2009-03-25 | 2010-09-25 | Sardinia Res And Dev S R L | HIPPOPHAE RHAMNOIDES EXTRACT AND COMPOUNDS: A NATURAL CURATIVE AGENT FOR BACTERIAL INFECTIONS OF MAN AND ANIMALS AS TUBERCULOSIS AND HEMORRAGIC SEPTICEMY. |
US8137714B2 (en) | 2008-07-25 | 2012-03-20 | Mary Kay Inc. | Compositions comprising docynia delavajy extract and/or Elaeagnus lancelotus extract |
RU2620006C1 (en) * | 2016-04-12 | 2017-05-22 | федеральное государственное бюджетное образовательное учреждение высшего образования "Восточно-Сибирский государственный университет технологий и управления" (ВСГУТУ) | Method for production of biologically active food additive based on sea buckthorn seeds |
EP3232814A4 (en) * | 2014-12-19 | 2018-08-29 | Teknologian tutkimuskeskus VTT Oy | Process for converting berry and fruit materials to antimicrobially active fractions |
RU2820697C1 (en) * | 2023-12-28 | 2024-06-07 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Восточно-Сибирский государственный университет технологий и управления" | Method of producing activated food additive based on sea-buckthorn fruits processing products |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102246999A (en) * | 2011-05-24 | 2011-11-23 | 上海创博食品技术发展有限公司 | Production method of natural preservative |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
SU1275032A1 (en) * | 1982-12-27 | 1986-12-07 | Тбилисский Ордена Трудового Красного Знамени Государственный Медицинский Институт | Method of producing sea buckthorn oil from press |
-
2003
- 2003-03-31 AU AU2003226634A patent/AU2003226634A1/en not_active Abandoned
- 2003-03-31 CN CN038263602A patent/CN1767843B/en not_active Expired - Fee Related
- 2003-03-31 DE DE10394212T patent/DE10394212T5/en not_active Ceased
- 2003-03-31 WO PCT/IN2003/000105 patent/WO2004087185A1/en not_active Application Discontinuation
-
2005
- 2005-09-30 FI FI20050980A patent/FI20050980A/en unknown
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
SU1275032A1 (en) * | 1982-12-27 | 1986-12-07 | Тбилисский Ордена Трудового Красного Знамени Государственный Медицинский Институт | Method of producing sea buckthorn oil from press |
Non-Patent Citations (3)
Title |
---|
DATABASE CA [online] CHEMICAL ABSTRACTS SERVICE, COLUMBUS, OHIO, US; ISAMUKHAMEDOV, A. SH. ET AL: "Hippophae rhamnoides seed phospholipids", XP009024685, retrieved from STN Database accession no. 97:88728 * |
DATABASE WPI Section Ch Week 198732, Derwent World Patents Index; Class D23, AN 1987-226820, XP002268596 * |
KHIMIYA PRIRODNYKH SOEDINENII (1982), (3), 395-6 * |
Cited By (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2168587A1 (en) * | 2007-06-13 | 2010-03-31 | Qinghai Tsinghua Biotry Bio-tech Co., Ltd | A method for preparing a mixture containing natural vitamin p from hippophae rhamnoides l. |
EP2168587A4 (en) * | 2007-06-13 | 2011-11-23 | Qinghai Tsinghua Biotry Bio Tech Co Ltd | A method for preparing a mixture containing natural vitamin p from hippophae rhamnoides l. |
US8137714B2 (en) | 2008-07-25 | 2012-03-20 | Mary Kay Inc. | Compositions comprising docynia delavajy extract and/or Elaeagnus lancelotus extract |
US9040104B2 (en) | 2008-07-25 | 2015-05-26 | Mary Kay Inc. | Compositions comprising Docynia delavajy extract and/or Elaeagnus lancelotus extract |
US9498427B2 (en) | 2008-07-25 | 2016-11-22 | Mary Kay Inc. | Compositions comprising Elaeagnus lancelotus extract |
US9808417B2 (en) | 2008-07-25 | 2017-11-07 | Mary Kay Inc. | Compositions comprising Docynia delavajy extract and/or Elaeagnus lancelotus extract |
ITSS20090006A1 (en) * | 2009-03-25 | 2010-09-25 | Sardinia Res And Dev S R L | HIPPOPHAE RHAMNOIDES EXTRACT AND COMPOUNDS: A NATURAL CURATIVE AGENT FOR BACTERIAL INFECTIONS OF MAN AND ANIMALS AS TUBERCULOSIS AND HEMORRAGIC SEPTICEMY. |
EP3232814A4 (en) * | 2014-12-19 | 2018-08-29 | Teknologian tutkimuskeskus VTT Oy | Process for converting berry and fruit materials to antimicrobially active fractions |
RU2702860C2 (en) * | 2014-12-19 | 2019-10-11 | Текнологиан Туткимускескус Втт Ой | Method of converting berry and fruit materials into antimicrobial active fractions |
US11044935B2 (en) | 2014-12-19 | 2021-06-29 | Teknologian Tutkimuskeskus Vtt Oy | Process for converting berry and fruit materials to antimicrobially active fractions |
RU2620006C1 (en) * | 2016-04-12 | 2017-05-22 | федеральное государственное бюджетное образовательное учреждение высшего образования "Восточно-Сибирский государственный университет технологий и управления" (ВСГУТУ) | Method for production of biologically active food additive based on sea buckthorn seeds |
RU2820697C1 (en) * | 2023-12-28 | 2024-06-07 | Федеральное государственное бюджетное образовательное учреждение высшего образования "Восточно-Сибирский государственный университет технологий и управления" | Method of producing activated food additive based on sea-buckthorn fruits processing products |
Also Published As
Publication number | Publication date |
---|---|
CN1767843B (en) | 2011-02-02 |
CN1767843A (en) | 2006-05-03 |
DE10394212T5 (en) | 2006-03-16 |
FI20050980A (en) | 2005-09-30 |
AU2003226634A1 (en) | 2004-10-25 |
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