WO2004085644A3 - Method for producing recombinant organisms - Google Patents

Method for producing recombinant organisms Download PDF

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Publication number
WO2004085644A3
WO2004085644A3 PCT/EP2004/002881 EP2004002881W WO2004085644A3 WO 2004085644 A3 WO2004085644 A3 WO 2004085644A3 EP 2004002881 W EP2004002881 W EP 2004002881W WO 2004085644 A3 WO2004085644 A3 WO 2004085644A3
Authority
WO
WIPO (PCT)
Prior art keywords
chromatin assembly
expression
recombinant
functionality
plants
Prior art date
Application number
PCT/EP2004/002881
Other languages
French (fr)
Other versions
WO2004085644A2 (en
Inventor
Ralf Badur
Bernd Reiss
Original Assignee
Basf Plant Science Gmbh
Ralf Badur
Bernd Reiss
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Basf Plant Science Gmbh, Ralf Badur, Bernd Reiss filed Critical Basf Plant Science Gmbh
Publication of WO2004085644A2 publication Critical patent/WO2004085644A2/en
Publication of WO2004085644A3 publication Critical patent/WO2004085644A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Botany (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

The present invention relates to methods for producing a recom­binant eukaryotic organisms, preferably a recombinant plant, comprising the step of decreasing the chromatin assembly capacity of said organism. The method may be used for introducing sequences into the chromosomal DNA of a eukaryotic cell either by illegitimate insertion or, preferably, by homologous recombina­tion. The method results in increased transformation efficacy and/or increased frequency of homologous recombination. The invention furthermore relates to expression constructs suitable for expressing at least part of a nucleic acid sequence encoding for a chromatin assembly factor protein (e.g., in form of antisense or double-stranded RNA) suitable for decreasing expression or functionality of at least one chromatin assembly factor protein in plant or plant cells. Object of the invention are furthermore recombinant plant cells or plants comprising a decreased chromatin assembly capacity, preferably a decreased expression or functionality at least one chromatin assembly factor and the use of said recombinant plants for the production of food, feeds, seed, pharmaceuticals or fine chemicals.
PCT/EP2004/002881 2003-03-26 2004-03-19 Method for producing recombinant organisms WO2004085644A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US45757403P 2003-03-26 2003-03-26
US60/457,574 2003-03-26

Publications (2)

Publication Number Publication Date
WO2004085644A2 WO2004085644A2 (en) 2004-10-07
WO2004085644A3 true WO2004085644A3 (en) 2005-01-13

Family

ID=33098234

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2004/002881 WO2004085644A2 (en) 2003-03-26 2004-03-19 Method for producing recombinant organisms

Country Status (1)

Country Link
WO (1) WO2004085644A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112048487B (en) * 2020-08-10 2023-07-04 山东大学 Regulatory protein synthesized by amylase, coding gene and application thereof

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999051731A2 (en) * 1998-04-03 1999-10-14 Pioneer Hi-Bred International, Inc. Maize histone deacetylases and their use
WO2000054574A2 (en) * 1999-03-18 2000-09-21 The University Of Chicago Chemical and physical treatment that stimulate recombination
WO2001004285A2 (en) * 1999-07-13 2001-01-18 Pioneer Hi-Bred International, Inc. Maize msi polynucleotides and methods of use
WO2001083793A2 (en) * 2000-04-28 2001-11-08 Sangamo Biosciences, Inc. Targeted modification of chromatin structure
WO2002046443A2 (en) * 2000-12-06 2002-06-13 Pioneer Hi-Bred International, Inc. Transcriptional regulator nucleic acids, polypeptides and methods of use thereof
WO2004003014A2 (en) * 2002-06-27 2004-01-08 Novartis Forschungsstiftung, Zweigniederlassung Friedrich Miescher Institute For Biomedical Research Gene for increased somatic recombination
WO2004011644A1 (en) * 2002-07-30 2004-02-05 Riken Method of promoting homologous recombination of somatic cells and method of constructing specific antibody

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999051731A2 (en) * 1998-04-03 1999-10-14 Pioneer Hi-Bred International, Inc. Maize histone deacetylases and their use
WO2000054574A2 (en) * 1999-03-18 2000-09-21 The University Of Chicago Chemical and physical treatment that stimulate recombination
WO2001004285A2 (en) * 1999-07-13 2001-01-18 Pioneer Hi-Bred International, Inc. Maize msi polynucleotides and methods of use
WO2001083793A2 (en) * 2000-04-28 2001-11-08 Sangamo Biosciences, Inc. Targeted modification of chromatin structure
WO2002046443A2 (en) * 2000-12-06 2002-06-13 Pioneer Hi-Bred International, Inc. Transcriptional regulator nucleic acids, polypeptides and methods of use thereof
WO2004003014A2 (en) * 2002-06-27 2004-01-08 Novartis Forschungsstiftung, Zweigniederlassung Friedrich Miescher Institute For Biomedical Research Gene for increased somatic recombination
WO2004011644A1 (en) * 2002-07-30 2004-02-05 Riken Method of promoting homologous recombination of somatic cells and method of constructing specific antibody

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
GORDON-KAMM W ET AL: "Stimulation of the cell cycle and maize transformation by disruption of the plant retinoblastoma pathway", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 99, no. 18, 3 September 2002 (2002-09-03), pages 11975 - 11980, XP002959418, ISSN: 0027-8424 *
KAYA HIDETAKA ET AL: "FASCIATA genes for chromatin assembly factor-1 in Arabidopsis maintain the cellular organization of apical meristems", CELL, vol. 104, no. 1, 12 January 2001 (2001-01-12), pages 131 - 142, XP002290298, ISSN: 0092-8674 *

Also Published As

Publication number Publication date
WO2004085644A2 (en) 2004-10-07

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