WO2004083405A3 - Separation et accumulation de composants sub-cellulaires, et proteines qui en sont tirees - Google Patents

Separation et accumulation de composants sub-cellulaires, et proteines qui en sont tirees Download PDF

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Publication number
WO2004083405A3
WO2004083405A3 PCT/US2004/008655 US2004008655W WO2004083405A3 WO 2004083405 A3 WO2004083405 A3 WO 2004083405A3 US 2004008655 W US2004008655 W US 2004008655W WO 2004083405 A3 WO2004083405 A3 WO 2004083405A3
Authority
WO
WIPO (PCT)
Prior art keywords
separation
accumulation
methods
subcellular organelles
derived therefrom
Prior art date
Application number
PCT/US2004/008655
Other languages
English (en)
Other versions
WO2004083405A2 (fr
WO2004083405A8 (fr
Inventor
Zvi G Loewy
Original Assignee
Alfa Wassermann Inc
Zvi G Loewy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alfa Wassermann Inc, Zvi G Loewy filed Critical Alfa Wassermann Inc
Priority to CA002518555A priority Critical patent/CA2518555A1/fr
Priority to AU2004221348A priority patent/AU2004221348A1/en
Priority to EP04757676A priority patent/EP1608969A4/fr
Priority to JP2006507434A priority patent/JP2006520606A/ja
Publication of WO2004083405A2 publication Critical patent/WO2004083405A2/fr
Publication of WO2004083405A3 publication Critical patent/WO2004083405A3/fr
Publication of WO2004083405A8 publication Critical patent/WO2004083405A8/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Food Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)

Abstract

La présente invention concerne des méthodes de fractionnement du protéome par séparation et accumulation d'organites subcellulaires provenant d'un échantillon biologique de telle sorte que ces organites subcelluliares soient fortement enrichis, sensiblement purs et que leur intégrité structurelle et leurs fonctions soient bien préservées. Les méthodes de l'invention permettent de réduire la complexité du protéome et de détecter et d'isoler plus facilement des protéines difficiles à étudier, telles que des protéines de faible abondance. Les méthodes de l'invention concernant le pré-fractionnement du protéome tiré d'échantillons biologiques à- séparation parallèle et isolation d'organites subcellulaires par ultracentrigugation en flux continu sont par ailleurs facilement et efficacement extensibles par ajustement des paramètres du rotor de centrifugation tels que vitesse, taille ou géométrie.
PCT/US2004/008655 2003-03-19 2004-03-19 Separation et accumulation de composants sub-cellulaires, et proteines qui en sont tirees WO2004083405A2 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA002518555A CA2518555A1 (fr) 2003-03-19 2004-03-19 Separation et accumulation de composants sub-cellulaires, et proteines qui en sont tirees
AU2004221348A AU2004221348A1 (en) 2003-03-19 2004-03-19 Separation and accumulation of subcellular components, and proteins derived therefrom
EP04757676A EP1608969A4 (fr) 2003-03-19 2004-03-19 Separation et accumulation de composants sub-cellulaires, et proteines qui en sont tirees
JP2006507434A JP2006520606A (ja) 2003-03-19 2004-03-19 細胞内成分の分離および蓄積、およびそれに由来するタンパク質

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US45576703P 2003-03-19 2003-03-19
US60/455,767 2003-03-19
US74131303A 2003-12-19 2003-12-19
US10/741,313 2003-12-19

Publications (3)

Publication Number Publication Date
WO2004083405A2 WO2004083405A2 (fr) 2004-09-30
WO2004083405A3 true WO2004083405A3 (fr) 2005-04-07
WO2004083405A8 WO2004083405A8 (fr) 2005-11-17

Family

ID=33032702

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/008655 WO2004083405A2 (fr) 2003-03-19 2004-03-19 Separation et accumulation de composants sub-cellulaires, et proteines qui en sont tirees

Country Status (6)

Country Link
EP (1) EP1608969A4 (fr)
JP (1) JP2006520606A (fr)
KR (1) KR20050119128A (fr)
AU (1) AU2004221348A1 (fr)
CA (1) CA2518555A1 (fr)
WO (1) WO2004083405A2 (fr)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2496400A1 (fr) 2002-09-09 2004-03-18 Dana-Farber Cancer Institute, Inc. Peptides bh3 et leur methode d'utilisation
WO2007123791A2 (fr) 2006-03-31 2007-11-01 Dana-Farber Cancer Institute Procédés de détermination de la chimiosensibilité cellulaire
CN104023551A (zh) * 2011-11-04 2014-09-03 生物辐射实验室股份有限公司 细胞组分的同时纯化
US9766207B2 (en) 2011-11-04 2017-09-19 Bio-Rad Laboratories, Inc. Affinity methods and compositions employing electronic control of pH
EP2814597A4 (fr) 2012-02-15 2015-10-28 Bio Rad Laboratories Régulation électronique de ph et de force ionique
US9321012B2 (en) 2012-04-04 2016-04-26 Bio-Rad Laboratories, Inc. Electronic protein fractionation
US10393733B2 (en) 2012-09-19 2019-08-27 Dana-Farber Cancer Institute, Inc. Dynamic BH3 profiling
EP3047276B1 (fr) 2013-09-19 2023-06-14 Dana-Farber Cancer Institute, Inc. Procédé de profilage de bh3
WO2016114992A2 (fr) * 2015-01-13 2016-07-21 Alfa Wassermann, Inc. Procédés de purification de virus adéno-associé (aav) et/ou de virus adéno-associé recombinant (raav) et gradients et tampons de flux d'écoulement pour ceux-ci
CA2983022C (fr) 2015-04-27 2023-03-07 Dana-Farber Cancer Institute, Inc. Profilage bh3 a haut rendement : une technologie rapide et echelonnable pour un profil bh3 sur un faible nombre de cellules
KR20160133837A (ko) 2015-05-13 2016-11-23 고려대학교 산학협력단 pH 조절 시료를 전기영동법으로 분리하기 위한 미세소포체 분리장치
KR20160133826A (ko) 2015-05-13 2016-11-23 고려대학교 산학협력단 전기영동을 이용한 미세소포체 분리장치
US11149246B2 (en) 2016-12-29 2021-10-19 Shoreline Biome, Llc Methods for cell lysis and preparation of high molecular weight DNA from modified cells
EP3562576B1 (fr) * 2016-12-29 2023-09-06 Intus Biosciences, LLC Protocole de lyse combiné pour lyse cellulaire complète
WO2021215878A1 (fr) 2020-04-24 2021-10-28 고려대학교 산학협력단 Procédé d'isolement de microvésicule et dispositif d'isolation de microvésicule
AU2022364731A1 (en) * 2021-10-12 2024-05-02 Cytonus Therapeutics, Inc. Systems and methods for manufacturing of therapeutic cells

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020115056A1 (en) * 2000-12-26 2002-08-22 Goodlett David R. Rapid and quantitative proteome analysis and related methods
US6573039B1 (en) * 1997-02-27 2003-06-03 Cellomics, Inc. System for cell-based screening
US20030124606A1 (en) * 2001-11-30 2003-07-03 Bruker Daltonik Gmbh Protein mixture analysis by mass spectrometry
US20040107057A1 (en) * 2002-03-22 2004-06-03 Capaldi Roderick A Enhanced protein separation and analysis

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4217418A (en) * 1978-05-08 1980-08-12 Merck & Co., Inc. Recovery of small particles by flow centrifugation
CA2140455A1 (fr) * 1993-05-28 1994-12-08 William C. Lake Procede continu de centrifugation pour la separation de constituants biologiques de populations de cellules heterogenes
ATE530253T1 (de) * 1995-12-11 2011-11-15 Dendreon Corp Zusammensetzung, kit und verfahren zur zelltrennung

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6573039B1 (en) * 1997-02-27 2003-06-03 Cellomics, Inc. System for cell-based screening
US20020115056A1 (en) * 2000-12-26 2002-08-22 Goodlett David R. Rapid and quantitative proteome analysis and related methods
US20030124606A1 (en) * 2001-11-30 2003-07-03 Bruker Daltonik Gmbh Protein mixture analysis by mass spectrometry
US20040107057A1 (en) * 2002-03-22 2004-06-03 Capaldi Roderick A Enhanced protein separation and analysis

Also Published As

Publication number Publication date
AU2004221348A1 (en) 2004-09-30
JP2006520606A (ja) 2006-09-14
WO2004083405A2 (fr) 2004-09-30
KR20050119128A (ko) 2005-12-20
WO2004083405A8 (fr) 2005-11-17
CA2518555A1 (fr) 2004-09-30
EP1608969A4 (fr) 2007-06-06
EP1608969A2 (fr) 2005-12-28

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