WO2004083393A3 - Enhanced protein thermostability and temperature resistance - Google Patents

Enhanced protein thermostability and temperature resistance Download PDF

Info

Publication number
WO2004083393A3
WO2004083393A3 PCT/US2004/007843 US2004007843W WO2004083393A3 WO 2004083393 A3 WO2004083393 A3 WO 2004083393A3 US 2004007843 W US2004007843 W US 2004007843W WO 2004083393 A3 WO2004083393 A3 WO 2004083393A3
Authority
WO
WIPO (PCT)
Prior art keywords
cellular
proteins
applications
pfu
temperature resistance
Prior art date
Application number
PCT/US2004/007843
Other languages
French (fr)
Other versions
WO2004083393A2 (en
Inventor
Frank T Robb
Pongpan Laksanalamai
Original Assignee
Univ Maryland Biotech Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Maryland Biotech Inst filed Critical Univ Maryland Biotech Inst
Publication of WO2004083393A2 publication Critical patent/WO2004083393A2/en
Publication of WO2004083393A3 publication Critical patent/WO2004083393A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Small heat shock proteins, e.g., Pyrococcus fuiosus (Pfu-sHSP and/or Pfu-tsHSP), confer thermotolerance on cellular cultures and on proteins in cellular extracts during prolonged incubation at elevated temperature, demonstrating the ability to protect cellular proteins and maintain cellular viability under heat stress conditions. Such heat shock proteins are effective to combat enzymatic aggregation and intracellular precipitation during heat stress, and thereby enable enhancement of the utility and stability of enzymes in various applications, e.g., Taq polymerise in PCR applications, digestive enzymes in microbial degradative applications, etc.
PCT/US2004/007843 2003-03-17 2004-03-15 Enhanced protein thermostability and temperature resistance WO2004083393A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/390,292 2003-03-17
US10/390,292 US7247432B2 (en) 2000-04-14 2003-03-17 Enhanced protein thermostability and temperature resistance

Publications (2)

Publication Number Publication Date
WO2004083393A2 WO2004083393A2 (en) 2004-09-30
WO2004083393A3 true WO2004083393A3 (en) 2005-06-02

Family

ID=33029673

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/007843 WO2004083393A2 (en) 2003-03-17 2004-03-15 Enhanced protein thermostability and temperature resistance

Country Status (2)

Country Link
US (1) US7247432B2 (en)
WO (1) WO2004083393A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010005708A2 (en) * 2008-06-16 2010-01-14 University Of Maryland Biotechnology Institute Improved folding of recombinant proteins via co-expression of archaeal chaperones
CN112210624B (en) * 2020-10-30 2023-07-25 浙江省农业科学院 LAMP primer and kit for efficiently detecting three pathogens of trionyx sinensis in triple mode

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001079250A1 (en) * 2000-04-14 2001-10-25 University Of Maryland Biotechnology Institute Enhanced protein thermostability and temperature resistance

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4201181A1 (en) * 1992-01-17 1993-07-22 Boehringer Mannheim Gmbh PROCESS FOR STABILIZING PROTEINS

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001079250A1 (en) * 2000-04-14 2001-10-25 University Of Maryland Biotechnology Institute Enhanced protein thermostability and temperature resistance
US6579703B2 (en) * 2000-04-14 2003-06-17 University Of Maryland Biotechnology Institute Enhanced protein thermostability and temperature resistance

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
DATABASE GENBANK [online] 12 August 2001 (2001-08-12), LAKSANALAMAI P. ET AL: "PYROROCCUS FURIOSUS SMALL HEAT SHOCK PROTEIN GENE, COMPLETE CDS", Database accession no. (GI7963823) *

Also Published As

Publication number Publication date
US7247432B2 (en) 2007-07-24
US20040005597A1 (en) 2004-01-08
WO2004083393A2 (en) 2004-09-30

Similar Documents

Publication Publication Date Title
Bhatti et al. Studies on kinetics and thermostability of a novel acid invertase from Fusarium solani
Sanghvi et al. Mitigation of acrylamide by L-asparaginase from Bacillus subtilis KDPS1 and analysis of degradation products by HPLC and HPTLC
Gaonkar et al. Characterization of extracellular protease from the haloarcheon Halococcus sp. strain GUGFAWS-3 (MF425611)
WO2006032708A3 (en) Nucleic acid sequences encoding proteins associated with abiotic stress response and plant cells and plants with increased tolerance to environmental stress
de Oliveira et al. Biochemical characterization and kinetic/thermodynamic study of Aspergillus tamarii URM4634 β‐fructofuranosidase with transfructosylating activity
Mohamed et al. Purification and characterization of asparaginase from Phaseolus vulgaris seeds
Rishad et al. Optimised production of chitinase from a novel mangrove isolate, Bacillus pumilus MCB-7 using response surface methodology
AU2001255400A1 (en) Enhanced protein thermostability and temperature resistance
Selim et al. Thermostable alkaline halophilic-protease production by Natronolimnobius innermongolicus WN18
Mohan Rao et al. Purification, optimization of assay, and stability studies of dextransucrase isolated from Weissella cibaria JAG8
Ameri et al. Thermoalkalophilic lipase from an extremely halophilic bacterial strain Bacillus atrophaeus FSHM2: purification, biochemical characterization and application
WO2004083393A3 (en) Enhanced protein thermostability and temperature resistance
Akram et al. Acinetobacter indicus CCS-12: a new bacterial source for the production and biochemical characterization of thermostable chitinase with promising antifungal activity
Ma et al. Insight into the cold adaptation and hemicellulose utilization of Cladosporium neopsychrotolerans from genome analysis and biochemical characterization
Çalışkan-Özdemir et al. Partial purification and characterization of a thermostable phytase produced by thermotolerant aspergillus tubingensis TEM 37 isolated from hot spring soil in gediz geothermal field, Turkey
Kadowaki et al. Characterization of trehalase activities from the thermophilic fungus Scytalidium thermophilum
Fullana et al. Identification, recombinant production and partial biochemical characterization of an extracellular cold-active serine-metalloprotease from an Antarctic Pseudomonas isolate
Cowan et al. Ecology and biodiversity of cold‐adapted microorganisms
CA2432123A1 (en) Preservation of cells
Cairns et al. Characterization of acid invertase from the snow mould Monographella nivalis: a mesophilic enzyme from a psychrophilic fungus
Fahmy et al. Characterization of an exopolygalacturonase from Aspergillus niger
Lin et al. Purification of soybean amylase by superparamagnetic particles
WO2003068975A1 (en) ss-LACTAMASE/ANMINOGLYCOSIDES MODIFYING ENZYME GENES, THEIR FUSION GENE AND THE EXPRESSION PRODUCTS, THE COMPOSITION THEREOF, AND THEIR USES IN MEDICINE AND ENVIRONMENTAL PROTECTION
Yi-Qing et al. Effects of no-tillage and straw incorporation on soil enzyme activity during wheat growth
CN201938593U (en) Novel schoolbag

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BW BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE EG ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NA NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): BW GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DPEN Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed from 20040101)
122 Ep: pct application non-entry in european phase