AU2001255400A1 - Enhanced protein thermostability and temperature resistance - Google Patents

Enhanced protein thermostability and temperature resistance

Info

Publication number
AU2001255400A1
AU2001255400A1 AU2001255400A AU5540001A AU2001255400A1 AU 2001255400 A1 AU2001255400 A1 AU 2001255400A1 AU 2001255400 A AU2001255400 A AU 2001255400A AU 5540001 A AU5540001 A AU 5540001A AU 2001255400 A1 AU2001255400 A1 AU 2001255400A1
Authority
AU
Australia
Prior art keywords
cellular
proteins
applications
temperature resistance
heat shock
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001255400A
Inventor
Pongpan Laksanalamai
Frank T. Robb
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Maryland Biotechnology Institute UMBI
Original Assignee
University of Maryland Biotechnology Institute UMBI
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Maryland Biotechnology Institute UMBI filed Critical University of Maryland Biotechnology Institute UMBI
Publication of AU2001255400A1 publication Critical patent/AU2001255400A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Abstract

Small heat shock proteins, e.g., Pyrococcus fuiosus (Pfu-sHSP), confer thermotolerance on cellular cultures and on proteins in cellular extracts during prolonged incubation at elevated temperature, demonstrating the ability to protect cellular proteins and maintain cellular viability under heat stress conditions. Such heat shock proteins are effective to combat enzymatic aggregation and intracellular precipitation during heat stress, and thereby enable enhancement of the utility and stability of enzymes in various applications, e.g., Taq polymerase in PCR applications, digestive enzymes in microbial degradative applications, etc.
AU2001255400A 2000-04-14 2001-04-16 Enhanced protein thermostability and temperature resistance Abandoned AU2001255400A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US19727400P 2000-04-14 2000-04-14
US60197274 2000-04-14
PCT/US2001/012290 WO2001079250A1 (en) 2000-04-14 2001-04-16 Enhanced protein thermostability and temperature resistance

Publications (1)

Publication Number Publication Date
AU2001255400A1 true AU2001255400A1 (en) 2001-10-30

Family

ID=22728721

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001255400A Abandoned AU2001255400A1 (en) 2000-04-14 2001-04-16 Enhanced protein thermostability and temperature resistance

Country Status (7)

Country Link
US (1) US6579703B2 (en)
EP (1) EP1287014B1 (en)
AT (1) ATE381573T1 (en)
AU (1) AU2001255400A1 (en)
CA (1) CA2409347C (en)
DE (1) DE60131969D1 (en)
WO (1) WO2001079250A1 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7247432B2 (en) 2000-04-14 2007-07-24 University Of Maryland Biotechnology Institute Enhanced protein thermostability and temperature resistance
CN100424179C (en) 2001-11-19 2008-10-08 诺沃挪第克公司 Process for preparing insulin compounds
KR100516389B1 (en) 2003-09-08 2005-09-23 한국과학기술원 Composition for Protecting Proteins Degradation Comprising Small Heat Shock Proteins(sHSPs) and Method of Two-Dimensional Gel Electrophoresis Using the sHSPs
US9416408B2 (en) * 2005-08-29 2016-08-16 University Of Maryland, Baltimore Thermostabillization of DNA polymerase by protein folding pathway from a hyperthermophile Archaeon, Pyrococcus furiosus
US20110014676A1 (en) * 2007-06-29 2011-01-20 Battelle Memorial Institute Protein stabilization
GB201209041D0 (en) * 2012-05-22 2012-07-04 Imp Innovations Ltd Protein manipulation
US11450609B2 (en) 2020-05-29 2022-09-20 Taiwan Semiconductor Manufacturing Co., Ltd. Electro-migration reduction

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4201181A1 (en) 1992-01-17 1993-07-22 Boehringer Mannheim Gmbh PROCESS FOR STABILIZING PROTEINS

Also Published As

Publication number Publication date
US6579703B2 (en) 2003-06-17
US20020077459A1 (en) 2002-06-20
EP1287014A1 (en) 2003-03-05
EP1287014B1 (en) 2007-12-19
ATE381573T1 (en) 2008-01-15
WO2001079250A1 (en) 2001-10-25
DE60131969D1 (en) 2008-01-31
CA2409347A1 (en) 2001-10-25
CA2409347C (en) 2010-03-30
EP1287014A4 (en) 2005-07-27

Similar Documents

Publication Publication Date Title
Eggen et al. Characterization of pyrolysin, a hyperthermoactive serine protease from the archaebacterium Pyrococcus furiosus
Fincan et al. Purification and characterization of thermostable α-amylase from thermophilic Anoxybacillus flavithermus
Ebrahiminezhad et al. l-Asparaginase production by moderate halophilic bacteria isolated from Maharloo Salt Lake
Sanghvi et al. Mitigation of acrylamide by L-asparaginase from Bacillus subtilis KDPS1 and analysis of degradation products by HPLC and HPTLC
Kasana et al. Isolation of a psychrotrophic Exiguobacterium sp. SKPB5 (MTCC 7803) and characterization of its alkaline protease
Shafiei et al. Purification and characterization of a halophilic α-amylase with increased activity in the presence of organic solvents from the moderately halophilic Nesterenkonia sp. strain F
García-Fraga et al. Functional expression and characterization of a chitinase from the marine archaeon Halobacterium salinarum CECT 395 in Escherichia coli
Li et al. Characterization of an organic solvent-tolerant α-amylase from a halophilic isolate, Thalassobacillus sp. LY18
Selim et al. Thermostable alkaline halophilic-protease production by Natronolimnobius innermongolicus WN18
AU2001255400A1 (en) Enhanced protein thermostability and temperature resistance
Berikten et al. Optimization of solid-state fermentation for phytase production by Thermomyces lanuginosus using response surface methodology
Zhang et al. Purification and characterization of a novel and versatile α‐amylase from thermophilic Anoxybacillus sp. YIM 342
Mohan Rao et al. Purification, optimization of assay, and stability studies of dextransucrase isolated from Weissella cibaria JAG8
Pessoni et al. Purification and properties of exo-inulinases from Penicillium janczewskii growing on distinct carbon sources
de Oliveira et al. Biochemical characterization and kinetic/thermodynamic study of Aspergillus tamarii URM4634 β‐fructofuranosidase with transfructosylating activity
CN105132450A (en) Method for displaying trehalose synthase on bacillus subtilis spore capsid protein Cot surfaces
Wang et al. Purification and properties of an extracellular cold-active protease from the psychrophilic bacterium Pseudoalteromonas sp. NJ276
Ameri et al. Thermoalkalophilic lipase from an extremely halophilic bacterial strain Bacillus atrophaeus FSHM2: purification, biochemical characterization and application
Özçelik et al. Production of an alkaline protease using Bacillus pumilus D3 without inactivation by SDS, its characterization and purification
Lama et al. Purification and characterization of a protease produced by an aerobic haloalkaliphilic species belonging to the Salinivibrio genus
Andronopoulou et al. Multiple components and induction mechanism of the chitinolytic system of the hyperthermophilic archaeon Thermococcus chitonophagus
Akram et al. Acinetobacter indicus CCS-12: a new bacterial source for the production and biochemical characterization of thermostable chitinase with promising antifungal activity
Ma et al. Insight into the cold adaptation and hemicellulose utilization of Cladosporium neopsychrotolerans from genome analysis and biochemical characterization
WO2004083393A3 (en) Enhanced protein thermostability and temperature resistance
Ulucay et al. For biotechnological applications: Purification and characterization of recombinant and nanoconjugated xylanase enzyme from thermophilic Bacillus subtilis