WO2004078981A8 - TRANSPOSASES D'ELEMENTS GENETIQUES MOBILES mariner MUTANTES, NON PHOSPHORYLABLES ET HYPERACTIVES - Google Patents

TRANSPOSASES D'ELEMENTS GENETIQUES MOBILES mariner MUTANTES, NON PHOSPHORYLABLES ET HYPERACTIVES

Info

Publication number
WO2004078981A8
WO2004078981A8 PCT/FR2004/000161 FR2004000161W WO2004078981A8 WO 2004078981 A8 WO2004078981 A8 WO 2004078981A8 FR 2004000161 W FR2004000161 W FR 2004000161W WO 2004078981 A8 WO2004078981 A8 WO 2004078981A8
Authority
WO
WIPO (PCT)
Prior art keywords
hyperactive
phosphorylated
genetic elements
transposases
mobile genetic
Prior art date
Application number
PCT/FR2004/000161
Other languages
English (en)
Other versions
WO2004078981A1 (fr
Inventor
Yves Bigot
Corinne Auge-Gouillou
Marie-Helene Hamelin
Benjamin Brillet
Original Assignee
Centre Nat Rech Scient
Univ Francois Rabelais De Tour
Yves Bigot
Corinne Auge-Gouillou
Marie-Helene Hamelin
Benjamin Brillet
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre Nat Rech Scient, Univ Francois Rabelais De Tour, Yves Bigot, Corinne Auge-Gouillou, Marie-Helene Hamelin, Benjamin Brillet filed Critical Centre Nat Rech Scient
Priority to JP2006505659A priority Critical patent/JP2006518220A/ja
Priority to EP04704632A priority patent/EP1590460A1/fr
Priority to CA002514730A priority patent/CA2514730A1/fr
Priority to US10/543,718 priority patent/US7732209B2/en
Publication of WO2004078981A1 publication Critical patent/WO2004078981A1/fr
Publication of WO2004078981A8 publication Critical patent/WO2004078981A8/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention a pour objets des transposases mutantes, non phosphorylables, hyperactives, d'éléments génétiques mobiles mariner. L'invention concerne également des séquences nucléotidiques recombinantes codant de telles transposases. En outre, l'invention vise un procédé de production de ces transposases, ainsi que l'utilisation de ces dernières pour la transposition in vitro ou in vivo.
PCT/FR2004/000161 2003-01-28 2004-01-23 TRANSPOSASES D'ELEMENTS GENETIQUES MOBILES mariner MUTANTES, NON PHOSPHORYLABLES ET HYPERACTIVES WO2004078981A1 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2006505659A JP2006518220A (ja) 2003-01-28 2004-01-23 マリナー可動遺伝因子の突然変異、リン酸化不能および機能亢進性トランスポゼース
EP04704632A EP1590460A1 (fr) 2003-01-28 2004-01-23 Transposases d elements genetiques mobiles mariner mutantes, non phosphorylables et hyperactives
CA002514730A CA2514730A1 (fr) 2003-01-28 2004-01-23 Transposases d'elements genetiques mobiles mariner mutantes, non phosphorylables et hyperactives
US10/543,718 US7732209B2 (en) 2003-01-28 2004-01-23 Hyperactive, non-phosphorylated, mutant transposases of mariner mobile genetic elements

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0300905A FR2850395B1 (fr) 2003-01-28 2003-01-28 Transposases d'elements genetiques mobiles mariner mutantes, non phosphorylables et hyperactives
FR03/00905 2003-01-28

Publications (2)

Publication Number Publication Date
WO2004078981A1 WO2004078981A1 (fr) 2004-09-16
WO2004078981A8 true WO2004078981A8 (fr) 2005-09-09

Family

ID=32669262

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2004/000161 WO2004078981A1 (fr) 2003-01-28 2004-01-23 TRANSPOSASES D'ELEMENTS GENETIQUES MOBILES mariner MUTANTES, NON PHOSPHORYLABLES ET HYPERACTIVES

Country Status (6)

Country Link
US (1) US7732209B2 (fr)
EP (1) EP1590460A1 (fr)
JP (1) JP2006518220A (fr)
CA (1) CA2514730A1 (fr)
FR (1) FR2850395B1 (fr)
WO (1) WO2004078981A1 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2893951B1 (fr) * 2005-11-30 2008-02-29 Centre Nat Rech Scient Procede de production chez les procaryotes de transposases actives et stables d'elements genetiques mobiles mariner
FR2900935A1 (fr) * 2006-05-15 2007-11-16 Centre Nat Rech Scient Systemes de transposition recombinants hyperactifs derives du transposon mos-1
ES2334087B1 (es) * 2008-02-29 2011-01-27 Universidad De Jaen Vectores y usos del transposon mboumar.
EP3184632B1 (fr) 2009-02-26 2024-04-03 Poseida Therapeutics, Inc. Transposases piggybac hyperactives
CN103627684B (zh) * 2013-11-20 2016-07-06 浙江农林大学 人工优化的高活性Mariner-Like转座酶

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU5642800A (en) * 1999-06-01 2000-12-18 Inserm Method of transposon-mediated mutagenesis in the nematode LTiGTcaenorhabditisLT/iGT LTiGTelegansLT/iGT
US6368830B1 (en) * 1999-10-01 2002-04-09 President And Fellows Of Harvard College Hyperactive mutants of Himar1 transposase and methods for using the same
CN1300827A (zh) * 1999-12-22 2001-06-27 上海博德基因开发有限公司 一种新的多肽—人水手转座酶19和编码这种多肽的多核苷酸

Also Published As

Publication number Publication date
EP1590460A1 (fr) 2005-11-02
WO2004078981A1 (fr) 2004-09-16
US7732209B2 (en) 2010-06-08
FR2850395B1 (fr) 2007-12-14
CA2514730A1 (fr) 2004-09-16
FR2850395A1 (fr) 2004-07-30
JP2006518220A (ja) 2006-08-10
US20070031967A1 (en) 2007-02-08

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