WO2004067765A3 - Procede pour prendre des empreintes d'organismes a l'aide d'agents de coupure de brin - Google Patents

Procede pour prendre des empreintes d'organismes a l'aide d'agents de coupure de brin Download PDF

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Publication number
WO2004067765A3
WO2004067765A3 PCT/US2004/002720 US2004002720W WO2004067765A3 WO 2004067765 A3 WO2004067765 A3 WO 2004067765A3 US 2004002720 W US2004002720 W US 2004002720W WO 2004067765 A3 WO2004067765 A3 WO 2004067765A3
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WO
WIPO (PCT)
Prior art keywords
sample
nicking agents
analyzed
nucleic acid
portions
Prior art date
Application number
PCT/US2004/002720
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English (en)
Other versions
WO2004067765A2 (fr
Inventor
Ness Jeffrey Van
David J Galas
Ness Lori K Van
Original Assignee
Keck Graduate Inst
Ness Jeffrey Van
David J Galas
Ness Lori K Van
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Keck Graduate Inst, Ness Jeffrey Van, David J Galas, Ness Lori K Van filed Critical Keck Graduate Inst
Publication of WO2004067765A2 publication Critical patent/WO2004067765A2/fr
Publication of WO2004067765A3 publication Critical patent/WO2004067765A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Selon l'invention, un échantillon d'acide nucléique, caractéristique d'une source particulière, par ex. une bactérie, peut être analysé(e), afin de déterminer si cet échantillon particulier provient d'une source particulière. Le protocole d'analyse soumet l'échantillon à une réaction par coupure de brin, afin de produire une famille de fragments à brin coupé. Dans certains modes de réalisation, la réaction par coupure de brin s'effectue en présence d'une polymérase et de triphosphates de nucléoside, afin d'amplifier les fragments à brin coupé ou des parties desdits fragments. Les fragments à brin coupé ou les parties desdits fragments peuvent être analysé(e)s pour déterminer la source de l'échantillon d'acide nucléique.
PCT/US2004/002720 2003-01-29 2004-01-29 Procede pour prendre des empreintes d'organismes a l'aide d'agents de coupure de brin WO2004067765A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US44381103P 2003-01-29 2003-01-29
US60/443,811 2003-01-29

Publications (2)

Publication Number Publication Date
WO2004067765A2 WO2004067765A2 (fr) 2004-08-12
WO2004067765A3 true WO2004067765A3 (fr) 2006-09-28

Family

ID=32825374

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/002720 WO2004067765A2 (fr) 2003-01-29 2004-01-29 Procede pour prendre des empreintes d'organismes a l'aide d'agents de coupure de brin

Country Status (1)

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WO (1) WO2004067765A2 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4822801B2 (ja) 2005-10-24 2011-11-24 西川ゴム工業株式会社 変異型エンドヌクレアーゼ
JP6126381B2 (ja) * 2010-12-10 2017-05-10 国立大学法人東京工業大学 標的核酸の検出方法及びキット
EP2831278B1 (fr) 2012-03-28 2019-05-08 Somalogic, Inc. Aptamères dirigés contre pdgf et vegf et leur utilisation dans le traitement d'états à médiation par pdgf et vegf
CA3221709A1 (fr) 2013-09-09 2015-03-12 Somalogic Operating Co., Inc. Aptameres de pdgf et vegf presentant une stabilite amelioree et leur utilisation dans le traitement de maladies et de troubles medies par pdgf et vegf
JP6739339B2 (ja) 2014-01-15 2020-08-12 アボット・ラボラトリーズＡｂｂｏｔｔ Ｌａｂｏｒａｔｏｒｉｅｓ カバーされた配列変換ｄｎａおよび検出方法
WO2016059474A2 (fr) 2014-10-14 2016-04-21 Abbott Japan Co., Ltd Adn de conversion de séquence et amplificateur de signal comportant des séquences espaceurs de poly-adn et procédés de détection l'utilisant
CN108064312B (zh) 2014-12-24 2022-04-08 雅培实验室 基于具有靶扩增的信号放大dna级联反应的检测方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5500363A (en) * 1990-04-26 1996-03-19 New England Biolabs, Inc. Recombinant thermostable DNA polymerase from archaebacteria
US5614386A (en) * 1995-06-23 1997-03-25 Baylor College Of Medicine Alternative dye-labeled primers for automated DNA sequencing
US5641658A (en) * 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
US5874215A (en) * 1995-01-16 1999-02-23 Keygene N.V. Amplification of simple sequence repeats

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5500363A (en) * 1990-04-26 1996-03-19 New England Biolabs, Inc. Recombinant thermostable DNA polymerase from archaebacteria
US5641658A (en) * 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
US5874215A (en) * 1995-01-16 1999-02-23 Keygene N.V. Amplification of simple sequence repeats
US5614386A (en) * 1995-06-23 1997-03-25 Baylor College Of Medicine Alternative dye-labeled primers for automated DNA sequencing

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
COLLINS ET AL.: "DNA Fingerprinting of Mycobacterium bovis Strains by Restriction Fragment Analysis and Hybridization with Insertion Elements IS1081 and IS6110", J. OF CLINICAL MICROBIOLOGY, vol. 31, no. 5, May 1993 (1993-05-01), pages 1143 - 1147, XP003001112 *
SIEGERT ET AL.: "Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry for the Detection of Polymerase Chain Reaction Products containing 7-Deazapurine Moieties", ANALYICAL BIOCHEMISTRY, vol. 243, 1996, pages 55 - 65, XP002072977 *

Also Published As

Publication number Publication date
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