WO2004065606A3 - Glycerol kinase inhibition in transgenic plant cells - Google Patents

Glycerol kinase inhibition in transgenic plant cells Download PDF

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Publication number
WO2004065606A3
WO2004065606A3 PCT/GB2004/000007 GB2004000007W WO2004065606A3 WO 2004065606 A3 WO2004065606 A3 WO 2004065606A3 GB 2004000007 W GB2004000007 W GB 2004000007W WO 2004065606 A3 WO2004065606 A3 WO 2004065606A3
Authority
WO
WIPO (PCT)
Prior art keywords
transgenic plant
plant cells
glycerol kinase
kinase inhibition
glycerol
Prior art date
Application number
PCT/GB2004/000007
Other languages
French (fr)
Other versions
WO2004065606A2 (en
Inventor
Peter Eastmond
Original Assignee
Univ York
Peter Eastmond
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ York, Peter Eastmond filed Critical Univ York
Publication of WO2004065606A2 publication Critical patent/WO2004065606A2/en
Publication of WO2004065606A3 publication Critical patent/WO2004065606A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C12P7/20—Glycerol

Abstract

We describe a transgenic plant cell which is genetically modified to provide a regulatable copy of a gene encoding a glycerol kinase, said plant shows increased resistance to abiotic stress and elevated cellular glycerol content.
PCT/GB2004/000007 2003-01-22 2004-01-05 Glycerol kinase inhibition in transgenic plant cells WO2004065606A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0301415.6 2003-01-22
GBGB0301415.6A GB0301415D0 (en) 2003-01-22 2003-01-22 Glycerol kinase

Publications (2)

Publication Number Publication Date
WO2004065606A2 WO2004065606A2 (en) 2004-08-05
WO2004065606A3 true WO2004065606A3 (en) 2004-09-16

Family

ID=9951560

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2004/000007 WO2004065606A2 (en) 2003-01-22 2004-01-05 Glycerol kinase inhibition in transgenic plant cells

Country Status (2)

Country Link
GB (1) GB0301415D0 (en)
WO (1) WO2004065606A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2193202A2 (en) * 2007-09-21 2010-06-09 BASF Plant Science GmbH Plants with increased yield
CN104726505A (en) * 2015-03-31 2015-06-24 上海交通大学 Method for producing three-carbon compounds by using gene engineering cyanobacteria

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999028480A1 (en) * 1997-12-02 1999-06-10 E.I. Du Pont De Nemours And Company Method for the production of glycerol by recombinant organisms
WO2001021820A1 (en) * 1999-09-22 2001-03-29 National Research Council Of Canada Transgenic manipulation of sn-glycerol-3-phosphate and glycerol production with a feedback defective glycerol-3-phosphate dehydrogenase gene
US20020023281A1 (en) * 2000-01-27 2002-02-21 Jorn Gorlach Expressed sequences of arabidopsis thaliana

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999028480A1 (en) * 1997-12-02 1999-06-10 E.I. Du Pont De Nemours And Company Method for the production of glycerol by recombinant organisms
WO2001021820A1 (en) * 1999-09-22 2001-03-29 National Research Council Of Canada Transgenic manipulation of sn-glycerol-3-phosphate and glycerol production with a feedback defective glycerol-3-phosphate dehydrogenase gene
US20020023281A1 (en) * 2000-01-27 2002-02-21 Jorn Gorlach Expressed sequences of arabidopsis thaliana

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
HASEGAWA P M ET AL: "PLANT CELLULAR AND MOLECULAR RESPONSES TO HIGH SALINITY", ANNUAL REVIEW OF PLANT PHYSIOLOGY, ANUAL REVIEWS, PALO ALTO, CA,, US, vol. 51, 2000, pages 463 - 499,4PAGES, XP009015683, ISSN: 0066-4294 *
KANG LI ET AL: "Interplay of the Arabidopsis nonhost resistance gene NHO1 with bacterial virulence.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 100, no. 6, 18 March 2003 (2003-03-18), March 18, 2003, pages 3519 - 3524, XP002285009, ISSN: 0027-8424 (ISSN print) *
LU MING ET AL: "Arabidopsis NHO1 is required for general resistance against Pseudomonas bacteria", PLANT CELL, vol. 13, no. 2, February 2001 (2001-02-01), pages 437 - 447, XP002285010, ISSN: 1040-4651 *
NANJO T ET AL: "Antisense suppression of proline degradation improves tolerance to freezing and salinity in Arabidopsis thaliana", FEBS LETTERS, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 461, no. 3, 19 November 1999 (1999-11-19), pages 205 - 210, XP004260550, ISSN: 0014-5793 *

Also Published As

Publication number Publication date
WO2004065606A2 (en) 2004-08-05
GB0301415D0 (en) 2003-02-19

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