WO2004061126A3 - Nucleic acid hybridization assay using bridging sequence - Google Patents

Nucleic acid hybridization assay using bridging sequence Download PDF

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Publication number
WO2004061126A3
WO2004061126A3 PCT/US2003/009232 US0309232W WO2004061126A3 WO 2004061126 A3 WO2004061126 A3 WO 2004061126A3 US 0309232 W US0309232 W US 0309232W WO 2004061126 A3 WO2004061126 A3 WO 2004061126A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
sequence
acid hybridization
hybridization assay
amplification
Prior art date
Application number
PCT/US2003/009232
Other languages
French (fr)
Other versions
WO2004061126A2 (en
Inventor
Robert C Getts
James Kadushin
Jaime Schwalm
Kevin Howerton
Original Assignee
Datascope Investment Corp
Robert C Getts
James Kadushin
Jaime Schwalm
Kevin Howerton
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Datascope Investment Corp, Robert C Getts, James Kadushin, Jaime Schwalm, Kevin Howerton filed Critical Datascope Investment Corp
Priority to AU2003230736A priority Critical patent/AU2003230736A1/en
Publication of WO2004061126A2 publication Critical patent/WO2004061126A2/en
Priority to US10/951,549 priority patent/US20050202461A1/en
Publication of WO2004061126A3 publication Critical patent/WO2004061126A3/en
Priority to US11/584,751 priority patent/US20080033159A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Methods for assay and/or amplification of nucleic acid sequences. An amplification procedure is provided for use with RNA samples that do not have a poly A sequence on the 3 prime end of the messenger RNA as is the case for bacterial, and other total RNA sources. Further, a method is provided for coupling, via ligation, a nucleic acid sequence to the 5 prime end of a random or sequence specific primer or to the 3 prime or 5 prime end of a synthesized DNA probe or target sequence, preferably to enable labelling of the target sequence or amplification thereof.
PCT/US2003/009232 2000-03-08 2003-03-25 Nucleic acid hybridization assay using bridging sequence WO2004061126A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU2003230736A AU2003230736A1 (en) 2002-03-25 2003-03-25 Nucleic acid hybridization assay using bridging sequence
US10/951,549 US20050202461A1 (en) 2000-03-08 2004-09-27 Method for converting generic nucleic acid priming sequences
US11/584,751 US20080033159A1 (en) 2001-01-13 2006-10-20 Method for small volume nucleic acid synthesis

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US36743802P 2002-03-25 2002-03-25
US60/367,438 2002-03-25

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US10/951,549 Continuation US20050202461A1 (en) 2000-03-08 2004-09-27 Method for converting generic nucleic acid priming sequences
PCT/US2004/031804 Continuation-In-Part WO2005030984A2 (en) 2001-01-13 2004-09-27 Method for small volume nucleic acid synthesis

Publications (2)

Publication Number Publication Date
WO2004061126A2 WO2004061126A2 (en) 2004-07-22
WO2004061126A3 true WO2004061126A3 (en) 2005-05-26

Family

ID=32712861

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/009232 WO2004061126A2 (en) 2000-03-08 2003-03-25 Nucleic acid hybridization assay using bridging sequence

Country Status (2)

Country Link
AU (1) AU2003230736A1 (en)
WO (1) WO2004061126A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2006041159A1 (en) * 2004-10-14 2008-08-07 学校法人日本大学 Primer with promoter region added
WO2024138670A1 (en) * 2022-12-30 2024-07-04 深圳华大生命科学研究院 Splint sequence and use thereof in nucleic acid capture

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5627030A (en) * 1994-06-28 1997-05-06 Kalyx Biosciences Inc. Method of amplification for increasing the sensitivity of detecting nucleic acid-probe target hybrids
US5942609A (en) * 1998-11-12 1999-08-24 The Porkin-Elmer Corporation Ligation assembly and detection of polynucleotides on solid-support

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5627030A (en) * 1994-06-28 1997-05-06 Kalyx Biosciences Inc. Method of amplification for increasing the sensitivity of detecting nucleic acid-probe target hybrids
US5942609A (en) * 1998-11-12 1999-08-24 The Porkin-Elmer Corporation Ligation assembly and detection of polynucleotides on solid-support

Also Published As

Publication number Publication date
WO2004061126A2 (en) 2004-07-22
AU2003230736A8 (en) 2004-07-29
AU2003230736A1 (en) 2004-07-29

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