WO2004055177A3 - Artificial esterases - Google Patents

Artificial esterases Download PDF

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Publication number
WO2004055177A3
WO2004055177A3 PCT/EP2003/013852 EP0313852W WO2004055177A3 WO 2004055177 A3 WO2004055177 A3 WO 2004055177A3 EP 0313852 W EP0313852 W EP 0313852W WO 2004055177 A3 WO2004055177 A3 WO 2004055177A3
Authority
WO
WIPO (PCT)
Prior art keywords
amino acid
esterases
artificial
artificial esterases
enantioselectivity
Prior art date
Application number
PCT/EP2003/013852
Other languages
French (fr)
Other versions
WO2004055177A2 (en
Inventor
Uwe T Bornscheuer
Anna Musidlowska-Persson
Harald Trauthwein
Original Assignee
Degussa
Uwe T Bornscheuer
Anna Musidlowska-Persson
Harald Trauthwein
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Degussa, Uwe T Bornscheuer, Anna Musidlowska-Persson, Harald Trauthwein filed Critical Degussa
Priority to AU2003288237A priority Critical patent/AU2003288237A1/en
Publication of WO2004055177A2 publication Critical patent/WO2004055177A2/en
Publication of WO2004055177A3 publication Critical patent/WO2004055177A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)

Abstract

The invention relates to artificial esterases wherein, on the basis of the amino acid sequence of a pig liver esterase, at least one defined amino acid exchange has been effected, making it possible to modify the enantioselectivity of the enzyme and in some cases to improve the activity and temperature stability.
PCT/EP2003/013852 2002-12-13 2003-12-06 Artificial esterases WO2004055177A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003288237A AU2003288237A1 (en) 2002-12-13 2003-12-06 Artificial esterases

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10258327.7 2002-12-13
DE2002158327 DE10258327A1 (en) 2002-12-13 2002-12-13 New artificial esterase, useful e.g. in washing compositions and for enantioselective reaction or hydrolysis of carboxylic acid derivatives, comprises that it is derived from porcine liver esterase by site-specific mutation

Publications (2)

Publication Number Publication Date
WO2004055177A2 WO2004055177A2 (en) 2004-07-01
WO2004055177A3 true WO2004055177A3 (en) 2004-09-10

Family

ID=32336296

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2003/013852 WO2004055177A2 (en) 2002-12-13 2003-12-06 Artificial esterases

Country Status (3)

Country Link
AU (1) AU2003288237A1 (en)
DE (1) DE10258327A1 (en)
WO (1) WO2004055177A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7311496B2 (en) 2018-05-02 2023-07-19 天野エンザイム株式会社 Modified esterase and use thereof

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1626093A1 (en) * 2004-08-11 2006-02-15 Dow Global Technologies Inc. Process for the production of (S)-5-chloro-2-isopropylpent-4-enoic acid esters
AT502991A1 (en) * 2005-12-27 2007-07-15 Dsm Fine Chem Austria Gmbh NEW POLYPEPTIDE WITH ESTERASE ACTIVITY, AND RECOMBINANT ESTERASE AND THEIR USE
AT502992B1 (en) * 2005-12-27 2008-04-15 Dsm Fine Chem Austria Gmbh PROCESS FOR PREPARING ENANTIOMER-ENRICHED 2-ALKYL-5-HALOGEN-PENT-4-EN-CARBOXYLIC ACIDES BZW. carboxylic acid esters
AT502990A1 (en) * 2005-12-27 2007-07-15 Dsm Fine Chem Austria Gmbh NEW POLYPEPTIDE WITH ESTERASE ACTIVITY, AND RECOMBINANT ESTERASE AND THEIR USE
DE102007014742A1 (en) * 2007-03-23 2008-09-25 Evonik Degussa Gmbh Isoforms of pork liver esterase
PL2171061T3 (en) * 2007-07-04 2019-03-29 Patheon Austria Gmbh & Co Kg Preparation of an esterase
DK2421962T3 (en) 2009-04-24 2018-10-22 Dpx Holdings Bv Novagraaf Brevets, Batiment 02, 2 rue Sarah Bernhardt CS90017, F-92665 Asniéres-surSeine cedex, France

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002048322A2 (en) * 2000-12-12 2002-06-20 Degussa Ag Recombinant porcine liver esterases, their use and a method for the production thereof

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002048322A2 (en) * 2000-12-12 2002-06-20 Degussa Ag Recombinant porcine liver esterases, their use and a method for the production thereof

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
BORNSCHEUER U W ET AL: "DIRECTED EVOLUTION OF AN ESTERASE: SCREENING OF ENZYME LIBRARIES BASED ON PH-INDICATORS AND A GROWTH ASSAY", BIOORGANIC & MEDICINAL CHEMISTRY, ELSEVIER SCIENCE LTD, GB, vol. 7, no. 10, October 1999 (1999-10-01), pages 2169 - 2173, XP001018345, ISSN: 0968-0896 *
BORNSCHEUER UWE T: "Methods to increase enantioselectivity of lipases and esterases.", CURRENT OPINION IN BIOTECHNOLOGY, vol. 13, no. 6, December 2002 (2002-12-01), pages 543 - 547, XP002274689, ISSN: 0958-1669 *
DATABASE EMBL [online] XP002274691, retrieved from EBI Database accession no. AAO18272 *
DATABASE EMBL [online] XP002274692, retrieved from EBI Database accession no. X63323 *
DATABASE EMBL [online] XP002274693, retrieved from EBI Database accession no. Q29550 *
DATABASE EMBL [online] XP002274694, retrieved from EBI Database accession no. O97582 *
DAVID L ET AL: "Purification and molecular cloning of porcine intestinal glycerol-ester hydrolase--evidence for its identity with carboxylesterase", FEBS LETTERS, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 257, no. 1, O97582, 1 October 1998 (1998-10-01), pages 142 - 148, XP002209304, ISSN: 0014-5793, Retrieved from the Internet <URL:EBI> *
MATSUSHIMA M ET AL: "The nucleotide and deduced amino acid sequences of porcine liver proline- beta-naphthylamidase. Evidence for the identity with carboxylesterase", FEBS LETTERS, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 293, no. 1-2, Q29550, 18 November 1991 (1991-11-18), pages 37 - 41, XP002209303, ISSN: 0014-5793, Retrieved from the Internet <URL:EBI> *
MUSIDLOWSKA-PERSSON A ET AL: "Site directed mutagenesis of recombinant pig liver esterase yields mutants with altered enantioselectivity", TETRAHEDRON: ASYMMETRY, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 14, no. 10, 16 May 2003 (2003-05-16), pages 1341 - 1344, XP004439169, ISSN: 0957-4166 *
MUSIDLOWSKA-PERSSON ANNA ET AL: "Recombinant porcine intestinal carboxylesterase: cloning from the pig liver esterase gene by site-directed mutagenesis, functional expression and characterization.", PROTEIN ENGINEERING. ENGLAND DEC 2003, vol. 16, no. 12, December 2003 (2003-12-01), pages 1139 - 1145, XP002274690, ISSN: 0269-2139 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7311496B2 (en) 2018-05-02 2023-07-19 天野エンザイム株式会社 Modified esterase and use thereof

Also Published As

Publication number Publication date
DE10258327A1 (en) 2004-06-24
WO2004055177A2 (en) 2004-07-01
AU2003288237A1 (en) 2004-07-09

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