WO2004051233A1 - Appareil et procede de photo-fragmentation de biopolymeres - Google Patents

Appareil et procede de photo-fragmentation de biopolymeres Download PDF

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Publication number
WO2004051233A1
WO2004051233A1 PCT/JP2003/014981 JP0314981W WO2004051233A1 WO 2004051233 A1 WO2004051233 A1 WO 2004051233A1 JP 0314981 W JP0314981 W JP 0314981W WO 2004051233 A1 WO2004051233 A1 WO 2004051233A1
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Prior art keywords
biopolymer
information
photocleaving
wavelength
cut
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PCT/JP2003/014981
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English (en)
Japanese (ja)
Inventor
Katsutoshi Takahashi
Kazuhiko Fukui
Yutaka Akiyama
Kunio Awazu
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National Institute Of Advanced Industrial Science And Technology
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Priority to AU2003284664A priority Critical patent/AU2003284664A1/en
Publication of WO2004051233A1 publication Critical patent/WO2004051233A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/12—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general

Definitions

  • the present invention relates to a biopolymer photocleaving device and a biopolymer photocutting method. More specifically, the present invention relates to a biopolymer photocleaving apparatus and a biopolymer photocutting method using an infrared laser under normal pressure. Background art
  • proteases Degradation reactions using proteases are also indispensable for separating and identifying proteins in proteome research. That is, proteins separated by one-dimensional or two-dimensional polyacrylamide gel electrophoresis are usually strongly adsorbed to the gel, and it is difficult to elute them in solution for subsequent analysis. Therefore, the mainstream of proteome analysis is the in-gel digestion method (protease method), in which the protein adsorbed on the gel is directly incubated in a protease solution and decomposed to elute as a soluble peptide fragment.
  • Non-Patent Documents 2 and 3 a method of decomposing proteins by ultraviolet rays is known (for example, see Non-Patent Documents 2 and 3).
  • Non-Patent Document 1 Little et al. (Little, DP et al.), Analytical Chemistry (Anal. Chem.), 1994, Vol. 66, p. 2809-2815.
  • Non-Patent Document 2 Bowes, W. D. et al., J. Am. Chem. Soc., 1998, 106, 106, p. 7 288—7289.
  • Non-Patent Document 3 Williams ER et al. ⁇ J. Am. So Mass Spectrom. 1990, vol. 1, p. 288-294. J. Am. So Mass Spectrom. '
  • the present invention focuses on and solves the above-mentioned problems of the conventional technology.
  • the object of the present invention is to provide a biomolecule capable of appropriately decomposing biomolecules such as proteins and polysaccharides without using enzymes.
  • An object of the present invention is to provide a polymer photocleaving apparatus and a biopolymer photocutting method. The present inventors have studied to solve the above problems, and as a result, have found that irradiation of an infrared laser under normal pressure can cut biopolymers appropriately, and have completed the present invention.
  • the biopolymer photocleaving device of the present invention includes: a support means for supporting the biopolymer sample under normal pressure; and an infrared laser-irradiating means for irradiating the biopolymer sample with an infrared laser. It is characterized by.
  • the biopolymer sample is supported by the support means under normal pressure. Then, the infrared polymer laser is irradiated to the biological polymer sample supported by the support under normal pressure by the infrared laser irradiation means.
  • the biopolymer photocleaving device of the present invention is an embodiment in which the infrared laser is a pulse laser in the biopolymer photocutting device described above.
  • a pulse laser infrared laser
  • the infrared laser irradiation means is irradiated by the infrared laser irradiation means to the biopolymer sample supported by the support means under normal pressure.
  • the wavelength of the infrared laser is a wavelength that resonates with the natural vibration of a chemical bond to be cut in the biopolymer. In one embodiment.
  • the biopolymer sample supported by the support means under normal pressure is applied at a wavelength that resonates with the intrinsic vibration of the chemical bond to be cut in the biopolymer.
  • An infrared laser is irradiated.
  • the biopolymer photocleaving device of the present invention is the biopolymer photocutting device described above, wherein information input means for inputting information of a portion of the biopolymer to be cut is provided; A storage unit for storing information of one wavelength of laser suitable for cutting, and referring to the storage unit, a laser having a wavelength corresponding to a site to be cut of the biopolymer inputted by the information input unit is stored. And a wavelength control means for irradiating the biopolymer sample.
  • this biopolymer photocleaving device information on the site where the biopolymer is to be cut is input by the information input means. Then, information of one laser wavelength suitable for cutting a specific portion of the biopolymer is stored in the storage means. With reference to the storage means Then, a laser having a wavelength corresponding to a portion of the biopolymer to be cut, which is input by the information input means, is applied to the biopolymer sample by the wavelength control means.
  • the biopolymer photocleaving device of the present invention there is provided the biopolymer photocutting device described above, wherein the mass spectrometry means for collecting a biopolymer sample after infrared laser irradiation and performing mass analysis, Analyzing the cut portion of the biopolymer cut based on the information obtained by the means, and storing the information obtained by the mass analysis means in the storage means together with the wavelength information of the irradiated infrared laser. And a means further comprising:
  • mass spectrometry is performed by collecting the biopolymer sample after irradiation with the infrared laser by the mass spectrometer.
  • the information analyzing means analyzes the cut portion of the cut biopolymer based on the information obtained by the mass analyzing means, and obtains the information along with the wavelength information of the irradiated infrared laser by the mass analyzing means.
  • the obtained information is stored in the storage means.
  • a mass spectrometer which collects a biopolymer sample after infrared laser irradiation and performs mass spectrometry; And information analysis means for transmitting information on the site where the next biopolymer is to be cut from the information obtained by the above to the wavelength control means.
  • the biopolymer sample after the irradiation with the infrared laser is recovered by the mass spectrometer and mass analysis is performed. Then, the information analyzing unit transmits the information of the site where the biopolymer is to be cut next from the information obtained by the mass analyzing unit to the wavelength control unit.
  • a laser wavelength suitable for cutting a specific portion of the biopolymer is characterized by a characteristic of a chemical bond of the portion to be cut. This is an embodiment having a wavelength that resonates with vibration.
  • the information of the site where the biopolymer is to be cut is input by the information input means. Then, information on a laser wavelength suitable for cutting a specific portion of the biopolymer is stored in the storage means.
  • the laser wavelength suitable for cutting a specific part of the biological polymer is determined by the chemical bonding of the part to be cut.
  • the wavelength having a wavelength that resonates with the natural vibration of the chemical bond at the site where the biopolymer is cut, which is input by the information input means, is referred to as the storage means.
  • the biopolymer sample is irradiated by the wavelength control means.
  • the biopolymer photocleaving device of the present invention is an embodiment of the above biopolymer photocleaving device, wherein the biopolymer is a protein or a polysaccharide.
  • a protein or polysaccharide is supported by the support means under normal pressure as a biopolymer to be cut. Then, the protein or polysaccharide held by the supporter under normal pressure is irradiated with an infrared laser by an infrared laser irradiation means.
  • the information on the site to be cleaved is information on at least one amino acid constituting a protein, or a polysaccharide.
  • the information of the site to be cleaved is information on at least one amino acid constituting the protein or information on at least one monosaccharide constituting the polysaccharide.
  • the information input means inputs information on at least one amino acid that constitutes a protein or information on at least one monosaccharide that constitutes a polysaccharide, as a report of the site to be cleaved in the biopolymer. Is done. Then, information of one wavelength of laser suitable for cutting a specific portion of the biopolymer (protein, polysaccharide, etc.) is stored in the storage means.
  • a laser having a wavelength corresponding to a site to be cut of a biological macromolecule (protein, polysaccharide, etc.) inputted by the information input means is input to the living body by the wavelength control means. Irradiates high molecular (protein, polysaccharide, etc.) samples. Further, the biopolymer photocleaving method of the present invention is characterized in that the biopolymer is cut by irradiating an infrared laser under normal pressure.
  • the biopolymer is irradiated with an infrared laser under normal pressure to cut the biopolymer.
  • the biopolymer photocleaving method of the present invention is an embodiment in which the infrared laser is a pulse laser in the above biopolymer photocleavage method.
  • the infrared laser is a pulse laser
  • the biopolymer is irradiated with a pulse laser (infrared laser) under normal pressure, and the pulse laser (infrared laser) is irradiated.
  • the biopolymer is cleaved.
  • the wavelength of the infrared laser is a wavelength that resonates with the intrinsic vibration of the chemical bond to be cut in the biopolymer. In one embodiment.
  • the wavelength of the infrared laser is the wavelength that resonates with the natural vibration of the chemical bond to be cut in the biopolymer, and the biopolymer is under normal pressure.
  • An infrared laser with a wavelength that resonates with the natural vibration of the chemical bond that is trying to sever the molecule is irradiated, and the biopolymer is severed.
  • the biopolymer photocleaving method of the present invention is an embodiment in which the biopolymer is a protein or a polysaccharide in the above biopolymer photocleavage method.
  • the biopolymer to be cleaved is protein or polysaccharide, and the biopolymer (protein, polysaccharide, etc.) is irradiated with an infrared laser. Macromolecules (proteins, polysaccharides, etc.) are cleaved.
  • FIG. 1 is a schematic configuration diagram of a first embodiment of the present invention.
  • FIG. 2 is a schematic configuration diagram of a second embodiment of the present invention.
  • FIG. 3 is a diagram illustrating a cleavage site of a protein.
  • FIG. 4 is a diagram showing a procedure for implementing the present invention (in the case of a liquid layer sample).
  • FIG. 5 is a diagram showing a procedure for implementing the present invention (for a solid sample).
  • FIG. 6 is a diagram showing main group vibrations in the IR wavelength region.
  • FIG. 7 is a diagram showing a relationship between a specific site of a biopolymer to be cut and a laser wavelength suitable for cutting the specific site.
  • FIG. 8 is a diagram showing the results of mass spectrometry of Substance P, which has not been irradiated with a laser, as a reference (R e fer en c e).
  • FIG. 3 is a diagram showing the results of mass spectrometry of FIG.
  • FIG. 10 is a view showing a difference spectrum of substance P cut by laser irradiation (wavelength: 6. lzm, 7.7 zm, 8.1 / m, 10.6 jum).
  • the biopolymer is not particularly limited as long as it is a polymer existing in an organism.
  • biopolymers such as proteins and polysaccharides, glycocop tin, proteoglycan, glycolipid, etc. Biopolymers can be mentioned.
  • the protein in the present invention refers to a protein in which a plurality of amino acids are peptide-bonded, and includes a dipeptide consisting of two amino acids, an oligopeptide, a polypeptide, and a protein having a molecular weight of more than 1000 kDa. Is also included.
  • a charged substance that is, a protein ion is also included in the protein of the present invention.
  • the protein in the present invention may be modified, and includes, for example, acetylated, methylated, phosphorylated, hydroxylated, formed disulfide bond, and added sugar chain. However, it is not limited to these.
  • the polysaccharide in the present invention means a carbohydrate that produces one or more monosaccharides by hydrolysis, and is a simple polysaccharide composed of the same kind of monosaccharide, and a complex polybran composed of different kinds of monosaccharide. Including both. Furthermore, glycoproteins, proteoglycans, glycolipids and the like bound to proteins are also included.
  • under normal pressure means a pressure other than vacuum, and includes not only the atmospheric pressure but also a pressure obtained by reducing or increasing the pressure to some extent.
  • the atmospheric pressure may be, for example, a pressure of 1.33 hPa (1 T rr) or more, and is preferably around the atmospheric pressure (10 13 hPa) because of the ease of the cutting process.
  • Specific examples include, but are not limited to, 700 to 1500 hPa, preferably 900 to 1 lOOhPa.
  • FIG. 1 shows an example of a biopolymer photocleaving device of the present invention.
  • the biopolymer optical cutting device of the present invention comprises a laser-oscillator 1, a shirt 1, a galvanomira 3, a power meter 4, a lens 5, a barium fluoride (B a F 2). ) Plate 6, sample 10, sample holder 7, and slide glass 8 are arranged in this order.
  • the laser-oscillator 1 constitutes the infrared laser irradiation means of the present invention
  • the barium fluoride (BaF 2 ) plate 6, the sample holder 7, and the slide glass 8 constitute the means for supporting the sample of the present invention. I do.
  • FIG. 1 one infrared laser beam emitted from a laser oscillator 1 is opened for a predetermined time by a shutter 12.
  • the laser light that has passed through the shutter 2 passes through the galvanomirror 3 and is condensed by the lens 5 to irradiate the sample 10.
  • the sample 10 is placed on the slide glass 8, the side surface is fixed by the sample holder 17, and the upper surface is covered by the B aF 2 plate 6.
  • the galvano mirror 13 has a function of irradiating the sample 10 by scanning one laser beam by changing the angle of a mirror provided therein. As a result, the sample 10 which is a biomolecule is cut.
  • the power meter 4 is inserted between the galvano mirror 13 and the lens 5 so that the laser light intensity can be measured.
  • a liquid layer sample is used as the sample 10, but the support means for supporting the sample used in the present invention is not particularly limited as long as the sample can be supported and irradiated with an infrared laser. Appropriate materials can be selected and used according to the layer sample and the liquid layer sample, respectively.
  • a target biopolymer is dropped on a slide glass surrounded by a sample holder, and the upper surface is coated with a glass.
  • infrared absorption such as barium reduction (B a F 2), potassium bromide (KB r) to cover one plate consisting of not less material, it is possible to support the sample remained liquid Wear.
  • the biopolymer after the biopolymer is cut by the infrared laser, it can be transferred to the next analysis step or the like in a solution state.
  • a separation carrier for biopolymers such as polyacrylamide gel can be used.
  • biopolymers such as proteins can be separated by polyallylamide gel electrophoresis, and the polyacrylamide gel on which the biopolymers are adsorbed can be used as a supporting means to directly support the sample. Can be advanced efficiently.
  • the infrared Les one The one irradiation means of the present invention is not particularly limited as long as it can emit infrared, for example, free electron laser and foremost, YAG laser one, can be mentioned C 0 2 laser Chief.
  • the wavelength of the infrared laser used in the present invention is not particularly limited as long as it can cut a chemical bond of a biopolymer, but from the viewpoint that it is possible to selectively cut a target chemical bond. It is preferable to use a wavelength that resonates with the natural vibration of the chemical bond to be broken.
  • the range of the wavelength actually used is, for example, 0.7 to 100 m, preferably 1 to 50 m.
  • the infrared laser used in the present invention it is preferable to use a pulse laser from the viewpoint of efficiently breaking the chemical bond of the biopolymer.
  • the target chemical bond can be cut efficiently by irradiating the laser with a pulse at a timing no longer than the vibration relaxation (attenuation).
  • the pulse width may be appropriately determined depending on the type of chemical bond to be cut. For example, a pulse width of several fsec (femtosecond) to several hundred psec (picosecond) can be used.
  • the irradiation energy of the infrared laser used in the present invention is not particularly limited as long as it is an energy capable of breaking a chemical bond of a biopolymer, and is, for example, 1 to 100 mJ, preferably 10 to 50 mJ. O m J.
  • FIG. 2 shows another example of the biopolymer photocleaving device of the present invention.
  • the control section 20 is configured by a computer including an information input section 30, a storage section 32, a wavelength control section 35, and an information analysis section 38.
  • the control unit 20 is connected to the laser oscillator 1 via the wavelength control unit 35. Further, the control unit 20 is connected to the mass analysis unit 40 via the information analysis unit 38. Other components are the same as in FIG.
  • the information input unit 30, the storage unit 32, the wavelength control unit 35, the information analysis unit 38, and the mass analysis unit 40 are the information input unit, storage unit, wavelength control unit, information analysis unit,
  • the respective mass spectrometers are configured. .
  • information on a site of a biopolymer to be cut is input to an information input unit 30.
  • the biopolymer is a protein
  • examples of information on the site to be cleaved include information on at least one amino acid constituting the protein or a sequence of at least two consecutive amino acids constituting the protein.
  • examples of information on the site to be cleaved include information on at least one monosaccharide constituting the polysaccharide or at least two consecutive monosaccharides constituting the polysaccharide. Sequence information.
  • the storage unit 32 stores information on a laser wavelength suitable for cutting a specific portion of the biopolymer.
  • a laser wavelength suitable for cutting a specific portion of a biopolymer is a wavelength that resonates with a natural vibration of a chemical bond of the cutting portion.
  • the wavelengths that resonate with these natural vibrations can be obtained by methods such as calculation simulation using the molecular orbital method and infrared absorption measurement using FT-IR. .
  • the wavelength control unit 35 refers to the storage unit 32 and transmits, from the laser-oscillator 1, the laser having the wavelength corresponding to the portion of the biopolymer to be cut, which is input by the information input unit 30. Radiate.
  • the biopolymer when the biopolymer is a protein, it becomes possible to selectively cleave the amino acid portion by inputting information on the amino acid at a specific site to be cleaved from the protein.
  • the wavelength giving the breathing mode of the 5-membered ring of proline (Breathing Mode) is 4 0 0 cm one 1 above calculation simulator - is determined by Chillon, Irradiating this wavelength gives energy to the five-membered ring of proline, which can transmit energy to the bond between histidine and proline by vibrational resonance, breaking this bond.
  • cleavage sites A, B, C, and D there are several cleavage sites as shown by cleavage sites A, B, C, and D in FIG.
  • cleavage sites A, B, C, and D histidine residues that Figure 3, the cleavage site A when R m is a proline residue, B, C, obtained in advance of the wavelength that resonates with the natural vibration of the D respectively, in the storage unit 3 2
  • R represents an amino acid residue
  • the mass spectrometer 40 collects the biopolymer sample after irradiation with the infrared laser and performs mass spectrometry.
  • the information obtained from the mass spectrometry unit 40 is sent to the information analysis unit 38, and the information analysis unit 3 In Fig. 8, the cut site of the biopolymer cut by the irradiation of the infrared laser is analyzed.
  • the analysis result (analysis result information) is stored in the storage unit 32 together with the information of the irradiated wavelength.
  • the analysis result information obtained by the information analysis unit 38 in the storage unit 32, it is possible to obtain more accurate information of a wavelength suitable for cutting a specific portion of the biopolymer. Or update the calculated wavelength suitable for cutting to the actually measured value.
  • the information analysis unit 38 can also transmit (instruct) information on the site where the next biopolymer is to be cut to the wavelength control unit 35.
  • the biopolymer is a protein
  • the information of “histidine-proline” is input to the information input unit 30 and the protein sample after the irradiation with the infrared laser is collected, and the mass spectrometer 40 collects the protein sample.
  • the information of the amino acid sequence to be next cut which is predetermined is transmitted (instructed) to the wavelength control unit 35, and the next infrared laser irradiation is performed. Can be performed.
  • the mass spectrometry means used in the present invention is not particularly limited as long as it can perform mass spectrometry. However, from the viewpoint that biopolymers can be accurately analyzed, MALDI-TOF is particularly preferable.
  • the substance P (SEQ ID NO: 1, SUBP) was cut by the following procedure using the biopolymer photocleaving apparatus shown in FIG.
  • substance P is known as a neuropeptide which is a chemical messenger released from nerve cells, and its amino acid sequence is “Arg—Pro—Lys — Pro— Gin— Gin— Phe— Phe— Gyl— Leu— Met ”.
  • substance P powder manufactured by SI GMA
  • Mi11iQ water is dissolved in Mi11iQ water to make a 2 pmo1 / L solution, and 10 ⁇ 1 ⁇ is placed in a sample holder as shown in Fig. 4 (13). The solution was dropped on a slide glass and covered with a BaF 2 plate.
  • a mixed solution of Mi11iQ water and methanol (50:50) containing 1% acetic acid ) samples were collected after the laser irradiation from the samples and B aF 2 based on slide Douglas was used as a 20 pmo 1Z ⁇ L solution.
  • the collected peptide was placed in a ⁇ pendorf tube, and then mass spectrometry was performed using 5 pmol / L diluted four-fold.
  • the mass spectrometry was performed using a tandem quadrupole 'time-of-flight hybrid type (qq_T ⁇ F) mass spectrometer (Applied Biosystems QSTAR PUL SAR).
  • substance P was dropped on a slide glass as a reference without irradiating a single laser, and was collected 30 minutes later, and mass spectrometry was performed.
  • irradiation experiments were performed using a tunable infrared laser.
  • the laser used at that time was characterized by a very short micropulse with a pulse width of about 5 ps, and this pulse was output for about 20 ms every 44.8 ns.
  • a macro pulse was formed from 450 of these micro pulses, and the macro pulse was output at 10 Hz.
  • the wavelength was adjusted to 7.7 jm, 8. l ⁇ m, and 10.6 m by this method, and the selectivity of the peptide cleavage site due to the wavelength dependence was investigated.
  • the laser beam intensity of the laser beam inserted between the galvanomirror and the lens is 12 to 15 mW (wavelength: 6. l ⁇ m), and 22 to 25 mW (wavelength : 7.7 to: 10.6 / m).
  • FIG. 10 also shows the difference spectrum when the laser light of each wavelength of 7.7 jm 8. lm and 10.6 ⁇ m was irradiated. In all these spectra, peaks appeared on the low mZz side, and the peaks could be identified as shown in Fig. 10 (c), confirming that substance P was decomposed. . From the above results, the utility of the biopolymer photocleaving apparatus and biopolymer photocleavage method of the present invention was confirmed.
  • the present invention it is possible to provide a biopolymer photocleaving apparatus and a biopolymer photocleaving method for cutting a biopolymer without using an enzyme.

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  • Investigating Or Analysing Materials By Optical Means (AREA)
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Abstract

Cette invention concerne un appareil de photo-fragmentation de biopolymères servant à fragmenter des biopolymères sans l'intervention d'enzyme, ainsi qu'un procédé de photo-fragmentation de biopolymères. Cet appareil de photo-fragmentation de biopolymères se caractérise en ce qu'il comprend une unité support servant à recevoir un échantillon de biopolymères à pression atmosphérique ainsi qu'une unité d'irradiation laser infrarouge servant à irradier l'échantillon de biopolymères avec des faisceaux laser infrarouges.
PCT/JP2003/014981 2002-11-29 2003-11-25 Appareil et procede de photo-fragmentation de biopolymeres WO2004051233A1 (fr)

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JP2002347786A JP3928043B2 (ja) 2002-11-29 2002-11-29 生体高分子光切断装置および生体高分子光切断方法

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JP2009103635A (ja) * 2007-10-25 2009-05-14 Univ Kansai 高分子化合物の分析方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH1164283A (ja) * 1997-08-22 1999-03-05 Jeol Ltd 大気圧レーザーイオン化質量分析装置及び方法
WO1999057318A2 (fr) * 1998-05-07 1999-11-11 Sequenom, Inc. Analyse de molecules par spectrometrie de masse a desorption/ionisation par laser, assistee par matrice absorbant l'infrarouge
JP2003344416A (ja) * 2002-05-28 2003-12-03 National Institute Of Advanced Industrial & Technology ポリペプチドがエネルギーにより切断される部位を予測する方法

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH1164283A (ja) * 1997-08-22 1999-03-05 Jeol Ltd 大気圧レーザーイオン化質量分析装置及び方法
WO1999057318A2 (fr) * 1998-05-07 1999-11-11 Sequenom, Inc. Analyse de molecules par spectrometrie de masse a desorption/ionisation par laser, assistee par matrice absorbant l'infrarouge
JP2003344416A (ja) * 2002-05-28 2003-12-03 National Institute Of Advanced Industrial & Technology ポリペプチドがエネルギーにより切断される部位を予測する方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
FUKUI K: "Jikken to keisanki simulation tono yugo ni yoru fragment dotei peptide setsudan no shitsuryo bunsekikei ni yoru kaiseki", AIST TODAY, vol. 02, no. 10, 1 October 2002 (2002-10-01), pages 12, XP002980161 *
FUKUI K: "Shototsu yuki kairi ya seigai takoshi kairi o tsukatta peptide setsudan no jikken riron", THE CHEM-BIO INFORMATICS ASSCIATION TAIKAI RONBUSHU, 18 September 2002 (2002-09-18), pages 85 - 86, XP002980160 *

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