WO2004047565A1

WO2004047565A1 - Agent for foods, drinks or medical uses containing body temperature-elevating amino acid

Info

Publication number: WO2004047565A1
Application number: PCT/JP2003/014740
Authority: WO
Inventors: Hajime Tsunoo; Masayuki Uchida; Takae Wakabayashi; Yasuko Sawai
Original assignee: Meiji Dairies Corporation
Priority date: 2002-11-22
Filing date: 2003-11-19
Publication date: 2004-06-10
Also published as: JP2006006101A; JP4316224B2; TW200418394A; AU2003302374A1

Abstract

It is intended to provide an agent for foods, drinks or medical uses which contains amino acid(s) elevating body temperature, characterized by containing tyrosine alone as the amino acid serving as the active ingredients or nothing but 4 amino acids including tyrosine, aspartic acid, glutamic acid and alanine as the amino acids serving as the active ingredients. The above agent is developed by confirming that tyrosine has an effect of elevating body temperature. By administering this agent, body temperature can be elevated and, in its turn, metabolic actions are promoted. As a result, body fat is burnt, thereby achieving effects of reducing accumulated body fat and relieving hypersensitivity to cold temperatures.

Description

Description Agents for foods and beverages or medicals containing amino acids that increase body temperature

Technical field to which the invention belongs

The present invention relates to a food or drink or medical agent effective for increasing body temperature. More specifically, the agent of the present invention comprises a specific amino acid, particularly tyrosine, as an active ingredient. It has an excellent physiological effect, especially an excellent body temperature increasing effect. Further, the agent according to the present invention promotes metabolism in the body by the action of increasing body temperature, thereby improving basal metabolism, further reducing body fat accumulation, improving coldness, and as a means for warming a body cooled in a cold region. Is also effective. Conventional technology

Conventionally, drinks containing cabsaicin as an active ingredient (for example, see Patent Document 1) have been known as focusing on the action of promoting the energy metabolism of pungent spice components. Patent Document 1

[Patent Document 1] Japanese Patent Application Laid-Open No. 2000-20089

Cabsaicins, which are said to have a body temperature increasing effect, are pungent components contained in pepper. If you add a large amount of capsaicin, you will feel strong spiciness. Had restrictions. In order to warm the body, a method of ingesting warm food and drink and taking in heat into the body can be considered, but this has a temporary effect. Therefore, a composition having an effect of improving energy and metabolism is required to be able to obtain the effect promptly, to continue its action after that, and to have good palatability when taken orally. Means for solving the problem

In order to achieve the above object, the present inventors have focused on a large number of amino acid compositions as a result of studies from various directions. A very large number of amino acid compositions were prepared by changing the types of amino acids, their combinations, and the amounts thereof, and the physiological effects of these amino acid compositions were thoroughly examined. As a result, they found for the first time an amino acid composition of a specific composition that raises basal body temperature and lasts for a long time, and as a result of further study of these amino acid group compositions, a composition with a high content of tyrosine was found. For the first time, I found that the body temperature was high V.

Therefore, the present inventors conducted a study focusing on this tyrosine, identified it as an amino acid that increased body temperature, confirmed the effect of increasing the body temperature of tyrosine only by this amino acid by an in vivo test, and conducted further studies. Finally, the present invention has been completed.

Furthermore, the present inventors have also confirmed for the first time that, in the course of the above method, when tyrosine was used in combination with aspartic acid, glutamic acid, and alanine, only these four amino acids exhibited an excellent body temperature increasing effect. As a result of further research, the present invention has finally been completed.

is there. Brief description of drawings ·

Figure 1

4 is a graph showing the average of the maximum basal body temperature increase when rats were administered tyrosine at each dose.

Figure 2

Similarly, it is a graph which shows the average of the basal body temperature rise temperature in each elapsed time. Fig 3

Similarly, it is a graph showing the average of the basal body temperature at each elapsed time.

Fig. 4

1 is a graph showing the average of the highest basal body temperature increase when the amino acid composition (four amino acids) according to the present invention is administered to rats.

Fig 5

Similarly, it is a graph which shows the average of the basal body temperature rise temperature in each elapsed time. Hereinafter, the present invention will be described in detail.

The present invention has been made for the first time to determine that the body of the body temperature increasing amino acid is tyrosine, wherein the active ingredient of the agent according to the present invention is tyrosine. Is a compound isolated or taken out independently and isolated as a single compound, and a commercially available product can be used as appropriate. In addition, the above-mentioned four kinds of amino acids (four kinds of amino acids) containing tyrosine (one kind of amino acids) also have an excellent body temperature increasing effect, and can be used as an active ingredient of the agent according to the present invention. In addition to tyrosine, various amino acids can be used, both natural products (including those produced by fermentation) and synthetic products. In the latter case, the L-form is preferable to the D-form, but the racemic form can also be used. is there. Therefore, in the agent according to the present invention containing only tyrosine as the active ingredient as the active ingredient, the active ingredient is only tyrosine, and in addition to tyrosine, other amino acids, peptides, foods and other body temperature increasing ingredients as active ingredients It does not refer to the combination of. The same applies to the case of four amino acids.

The agent according to the present invention contains tyrosine alone or only four amino acids of tyrosine, aspartic acid, glutamic acid, and alanine as active ingredients.The active ingredient of the agent according to the present invention is as follows: 1 and 2.

(1) One kind of amino acid: tyrosine

(2) Four amino acids: tyrosine + (aspartic acid, glutamic acid, aranine).

In the case of the above four amino acids (aspect 2), the mixing ratio (% by weight) of each amino acid can be, for example, as follows.

(Preferred area)

Tyrosine 40-65 (45-60)

Aspartic acid 0.1-; L 0 (0.5-5)

Glutamic acid 10-35 (15-30)

Alanine 10-40 (15-35)

(Of course, the above composition is shown as an example, and the tyrosine content can be further increased in the above composition.)

The agent according to the present invention contains tyrosine as an active ingredient. Tyrosine may be used alone or may coexist with other amino acids to form an amino acid group (four amino acids). Each amino acid is preferably contained in a specific composition ratio, and is shown below. That is, each amino acid is tyrosine 0.5 to 15 mol or more, aspartic acid 0.02 to 0.4 mol, gnoretamic acid 0.3 to 7 monole, and aranine 0.7 to 1

It is preferable to include it in a ratio of 4 mol.

Specific examples of tyrosine-containing amino acids (four amino acids (4AA)) constituting the active ingredient of the agent according to the present invention are shown in Table 1 below.

(table 1)

4 AA

Molecular weight m o 1

Γ y r 181.2 0.0475 8.6

As 133.1 0.0015 0.2

G 1 u 147.1 0.0252 3.7

A 1 a 89.1 0.0483 4.3 total 0.1225 16.8

T yr% 38.8 51.2 The food, drink, or medical agent related to the present study that has increased body temperature and contains only tyrosine or only four amino acids containing tyrosine as an amino acid is a component or an active ingredient. The active ingredient alone may be orally ingested in the form of a powder or an aqueous solution without or without mixing, but the contained or active ingredient may be liquid or solid and taste If it is not good, or if it is difficult to swallow, use the contained or active ingredients and additives that are commonly used for eating and drinking or medical use to improve the taste It is better to make it easier to drink or to make the whole biscuit easier to eat. For example, the drinks can be made easier only by adding a souring agent such as citric acid or a sweetener such as sugar to the aqueous liquid containing or containing the active ingredient. Addition of purified starch, etc. to the active ingredient in a powder-mixed state, mixing, baking, and biscuit form makes it possible to eat deliciously.

As described above, the food or drink or medical agent for increasing body temperature according to the present invention may be ingested as a powder or may be dissolved in water and ingested as an aqueous solution or the like. The ingestion method may be oral administration, rectal administration, intravenous injection, intravenous drip, and other general administration routes.

In the case of oral administration, in addition to making it easy to drink and eat for eating and drinking, it is mixed with pharmaceutically acceptable carriers, excipients and diluents, and powders, granules, tablets, forcepsels, It may be used as a mouthpiece. However, solid powders and tablets may require time for absorption, so it is preferable to administer the active ingredient orally. In this case, it may be administered as a solution as described above together with a suitable additive, for example, a salt such as sodium chloride, a pH adjusting agent, and a chelating agent. When used as an injection, an appropriate buffer and isotonic agent may be added and dissolved in sterile distilled water.

There is no particular restriction on the timing of ingestion, and it may be taken at any time. It is preferable to ingest as In the case of drinks, it is preferable to formulate tyrosine as an active ingredient so as to contain at least 25 mg, preferably at least 250 mg, more preferably at least 2.5 g per 2 OO m1 of the drink. is there.

The dosage of the food or drink or medical agent of the present invention can be set in a wide range, For oral administration, 0.1 to 5 g of active ingredient is usually administered at a time depending on the method and purpose of use. When administered or ingested as a solution is 10 to once as a solution of about 0.05 to 10 wt ° / ₀ 1000 m l, preferably 100 to 500 meters l administration or once a 1-4 wt ° / ₀ Ingest.

As will be apparent from the examples described below, the food or drink or medical agent according to the present invention has an excellent basal body temperature-increasing action, increases basal metabolism, and consumes energy in daily life. As a result, body fat is burned, and as a result, it is also effective in reducing body fat accumulation and improving coldness. Therefore, various pharmaceuticals such as a body temperature increasing agent and an anti-chilling agent can be prepared by formulating the active ingredient as a medicament.

The food or drink or medical agent according to the present invention has an extremely effective effect of increasing body temperature, and when used, it may be used as a powder. In this case, the composition of the present invention may be dissolved directly in water to prepare a solution, or each amino acid may be separately dissolved in water to realize the above-described composition in the solution.

Hereinafter, examples of the present invention will be described.

Example 1

(1) Method

Six SD male rats of 8 to 10 weeks of age were prepared in groups of six, and after fasting overnight, they were used for experiments. The groups were divided according to the weight on the day of use.

(2) Test substance

As test substances, four kinds of amino acids (tyrosine, aspartic acid, glutamic acid, alanine) and one kind of amino acid (tyrosine) were used. The mixing ratio is as shown in Table 1, but the mixing ratio of the four amino acids is as follows. The amino acid (tyrosine) was determined based on the mixing ratio of the four amino acids. In addition, all amino acids were obtained from Wako Pure Chemical Industries, Ltd.

4 amino acids Amino acid Component weight (%) Mole (%) Tyrosine 50.9 38.5

Aspartic acid 1.1 1.1

Gunoretamic acid 22.3 20.8

Alanine 25.7 39.6

(3) Dosage

One group was used as a control (water for injection). For the two groups, the four amino acids were orally administered once at 0.5 g / 100 g body weight (5 g / kg). Groups 3 to 5 were treated with a single amino acid group, tyrosine 0.25 g / 10 Og body weight (2.5 g / kg) for group 3, and tyrosine 0. Oral administration of tyrosine once per 25 g / 100 g body weight (1.25 g / kg), and also for each of the 5 groups at a dose of 0.0625 gZ per 100 g body weight (0.625 g / kg) did. For groups 3 to 5, one group was prepared with a control (water for injection), and the test was performed. .

The test substance amino acid was suspended in physiological saline, and only saline (Hikari Pharmaceutical Co., Ltd., Japanese Pharmacopoeia physiological saline, Lot. 9911HC) was administered to the control port. . (4) Body temperature measurement

The animals were kept in a ballman cage and a probe for measuring the body temperature was inserted into the rectum from the anus and fixed. Measurements were taken every 10 to 15 minutes. When the body temperature stabilized, the test substance was administered, and the body temperature was measured every 10 minutes until 90 minutes later, based on the temperature at the time of ingestion 0 minutes. The average was taken from the results for each group, and the value was shown on the graph. In addition, the maximum value of the elevated body temperature for each individual was averaged for each group, and the difference between each group was shown.

That is, the average of the maximum body temperature increase at each dose of one amino acid (tyrosine) is shown in Fig. 1, and the average of the body temperature elevation temperature and the average of the body temperature at each elapsed time are shown in Figs. 2 and 3, respectively. .

For the four amino acids, the average of the maximum body temperature rise is shown in FIG. 4, and the average of the body temperature rise temperature at each time is shown in FIG.

(5) Results and discussion

As is clear from the above results, the temperature of the control group slightly increased with time. The test substance-administered group (one tyrosine-administered group) showed a 用量 mabo dose-dependent increase in body temperature, and the results of T-test and F-test were significant in two groups (four amino acids) compared to the control group. Significant differences were observed.

Example 2

2.6 kg of tyrosine, 1 kg of citrate, 2 kg of sucrose, 1 kg of trehalose, 1 kg of fragrance, and 92.4 kg of water were heated and dissolved and mixed to prepare a liquid containing amino acids with increasing body temperature. This was packed in bottles of 2 O Oml each time and sterilized to give a body temperature-increasing amino acid-containing drink.

Example 3

Tyrosine 1.3 kg, aspartic acid 0.03 kg, glutamic acid 0.58 kg, alanine 0.67 kg, citrate 1 ¾: 3, sucrose 21 ^ §, treja 1 kg of loin, 1 kg of fragrance, and 92.42 kg of water were mixed by heating and dissolved to produce a body temperature-raising amino acid-containing liquid. This was packed in bottles of 200 ml per serving, sterilized, and used as a drink containing aminoamino acid with increased body temperature. The invention's effect

The present inventors have found a novel use of an active ingredient composed of a specific amino acid group as having an excellent body temperature increasing effect, and have also clarified, for the first time, that the main body thereof is chin mouth. The body temperature-raising food or drink or medical agent containing tyrosine alone or a group of four amino acids containing tyrosine according to the present work can reduce the body temperature in daily life by administering it. It has the significant effect of increasing energy consumption and increasing basic metabolism. It is also effective in raising basal body temperature, lowering body fat accumulation and improving coldness, etc. It has low toxicity and can be taken for a long time.

Claims

The scope of the claims

1. An agent for foods or drinks or medicals containing an amino acid that has increased body temperature, comprising only tyrosine as an amino acid.

2. An agent for foods, drinks, or medicals containing an amino acid with an increased body temperature, characterized by containing only tyrosine, aspartic acid, glutamic acid, and alanine as amino acids.

3. An agent for foods and drinks or medicals containing an amino acid with a temperature-increasing temperature, comprising tyrosine as an active ingredient as an amino acid.

4. An agent for foods, drinks or medicines containing an amino acid with a temperature-increasing amino acid, characterized by containing tyrosine, aspartic acid, glutamic acid, and alanine as amino acids as active ingredients.

5. The agent according to any one of claims 1 to 4, comprising at least 25 mg of tyrosine per 20 Om1.

6. The agent according to claim 2 or 4, wherein tyrosine is contained in an amount of 40% by weight or more in the amino acid group.

7. A food or beverage containing an amino acid with an increase in body temperature, characterized by comprising only the amino acid or the amino acid group according to any one of claims 1 to 6 and an additive commonly used for eating or drinking or for medical use. Product or medical agent.