WO2004046730A3 - Uses of ble proteins and antibiotics from the bleomycin family - Google Patents

Uses of ble proteins and antibiotics from the bleomycin family Download PDF

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Publication number
WO2004046730A3
WO2004046730A3 PCT/IB2003/005430 IB0305430W WO2004046730A3 WO 2004046730 A3 WO2004046730 A3 WO 2004046730A3 IB 0305430 W IB0305430 W IB 0305430W WO 2004046730 A3 WO2004046730 A3 WO 2004046730A3
Authority
WO
WIPO (PCT)
Prior art keywords
protein
bleomycin family
antibiotic
ble
bleomycin
Prior art date
Application number
PCT/IB2003/005430
Other languages
French (fr)
Other versions
WO2004046730A2 (en
Inventor
Darren Hart
Ben Godber
Jonathan M Blackburn
Mike Mcandrews
Original Assignee
Sense Therapeutic Ltd
Darren Hart
Ben Godber
Jonathan M Blackburn
Mike Mcandrews
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0224872A external-priority patent/GB0224872D0/en
Priority claimed from GB0229640A external-priority patent/GB2385327A/en
Priority claimed from GB0307379A external-priority patent/GB2400102A/en
Application filed by Sense Therapeutic Ltd, Darren Hart, Ben Godber, Jonathan M Blackburn, Mike Mcandrews filed Critical Sense Therapeutic Ltd
Priority to JP2004570323A priority Critical patent/JP2006506447A/en
Priority to EP03811464A priority patent/EP1565754B1/en
Priority to AU2003302133A priority patent/AU2003302133A1/en
Priority to US10/532,834 priority patent/US20060194715A1/en
Priority to DE60315343T priority patent/DE60315343T2/en
Priority to DK03811464T priority patent/DK1565754T3/en
Publication of WO2004046730A2 publication Critical patent/WO2004046730A2/en
Publication of WO2004046730A3 publication Critical patent/WO2004046730A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
    • G01N33/9446—Antibacterials
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/68—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an antibody, an immunoglobulin or a fragment thereof, e.g. an Fc-fragment
    • A61K47/6801—Drug-antibody or immunoglobulin conjugates defined by the pharmacologically or therapeutically active agent
    • A61K47/6803—Drugs conjugated to an antibody or immunoglobulin, e.g. cisplatin-antibody conjugates
    • A61K47/6807—Drugs conjugated to an antibody or immunoglobulin, e.g. cisplatin-antibody conjugates the drug or compound being a sugar, nucleoside, nucleotide, nucleic acid, e.g. RNA antisense
    • A61K47/6809—Antibiotics, e.g. antitumor antibiotics anthracyclins, adriamycin, doxorubicin or daunomycin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2415/00—Assays, e.g. immunoassays or enzyme assays, involving penicillins or cephalosporins

Abstract

The present invention provides use of a protein conjugate comprising a “ble” protein, which has specific binding properties. The protein conjugates are capable of binding reversibly to an antibiotic from the bleomycin family, which property is exploited in a variety of immobilisation methods. In preferred aspects of the invention, the conjugates are used as markers for protein expression and/or folding, or for affinity tagging. The present invention also provides a probe comprising an array of an immobilised antibody from the bleomycin family, which acts as an analyte capture moiety. In another aspect, a purification media is provided, which comprises an antibiotic of the bleomycin family as an analyte capture moiety. Also provided is a method for generating soluble forms of an insoluble protein by expressing the protein as a “ble” fusion protein and selecting in the presence of an antibiotic from the bleomycin family. In a further aspect, the “ble” protein is expressed as a fusion protein in a cell into which is introduced a labelled antibiotic of the bleomycin family, thereby allowing identification of the cellular localisation of the protein.
PCT/IB2003/005430 2002-10-25 2003-10-27 Uses of ble proteins and antibiotics from the bleomycin family WO2004046730A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
JP2004570323A JP2006506447A (en) 2002-10-25 2003-10-27 Proteins encoded by the BLE gene and novel methods for using bleomycin family-derived antibiotics
EP03811464A EP1565754B1 (en) 2002-10-25 2003-10-27 New uses of proteins encoded by ble genes and antibiotics from the bleomycin family
AU2003302133A AU2003302133A1 (en) 2002-10-25 2003-10-27 Uses of ble proteins and antibiotics from the bleomycin family
US10/532,834 US20060194715A1 (en) 2002-10-25 2003-10-27 Proteins encoded by ble genes and antibiotics from the bleomycin family
DE60315343T DE60315343T2 (en) 2002-10-25 2003-10-27 NOVEL USES OF BLE GENES PROTEINS AND ANTIBIOTICS FROM THE BLEOMYCIN FAMILY
DK03811464T DK1565754T3 (en) 2002-10-25 2003-10-27 New uses of proteins encoded by the BLE genes and antibiotics from the bleomycin family

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
GB0224872A GB0224872D0 (en) 2002-10-25 2002-10-25 Probe for mass spectrometry
GB0224872.2 2002-10-25
GB0229640A GB2385327A (en) 2001-12-21 2002-12-20 Probe for mass spectrometry
GB0229640.8 2002-12-20
GB0307379.8 2003-03-31
GB0307379A GB2400102A (en) 2003-03-31 2003-03-31 Uses of Ble proteins and bleomycin

Publications (2)

Publication Number Publication Date
WO2004046730A2 WO2004046730A2 (en) 2004-06-03
WO2004046730A3 true WO2004046730A3 (en) 2004-09-02

Family

ID=32329543

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2003/005430 WO2004046730A2 (en) 2002-10-25 2003-10-27 Uses of ble proteins and antibiotics from the bleomycin family

Country Status (9)

Country Link
US (1) US20060194715A1 (en)
EP (1) EP1565754B1 (en)
JP (2) JP2006506447A (en)
AT (1) ATE368861T1 (en)
AU (1) AU2003302133A1 (en)
DE (1) DE60315343T2 (en)
DK (1) DK1565754T3 (en)
ES (1) ES2290556T3 (en)
WO (1) WO2004046730A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8846320B2 (en) 2006-11-01 2014-09-30 Ventana Medical Systems, Inc. Haptens, hapten conjugates, compositions thereof and method for their preparation and use

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7094568B2 (en) * 2000-08-17 2006-08-22 Sense Proteomic Ltd. Method for producing proteins tagged at the N- or C-terminus
US20040002078A1 (en) * 2001-12-05 2004-01-01 Sense Proteomic Limited Arrays
CN101199866B (en) * 2007-12-27 2010-09-22 高景星 Soluble cotton fiber containing multiple bioactivity and preparing method thereof
GB2460717A (en) 2008-06-11 2009-12-16 Sense Proteomic Ltd Autoantibodies for the detection of a predisposition to Lupus
GB2478734A (en) 2010-03-15 2011-09-21 Sense Proteomic Ltd Auto-antibody biomarkers of prostate cancer
GB201017520D0 (en) 2010-10-15 2010-12-01 Sense Proteomic Ltd Biomarkers
JP6321622B2 (en) 2012-03-30 2018-05-09 ビーディー キエストラ ベスローテン フェンノートシャップ Automated selection of microorganisms and identification using MALDI
JP6231263B2 (en) * 2012-07-17 2017-11-15 株式会社島津製作所 Affinity support and method of capturing a substance using the same
CN104569325B (en) * 2014-12-26 2016-11-23 华中农业大学 For detecting antibody chip test kit and the method for aminoglycoside antibiotics residual in food
CN112116950B (en) * 2020-09-10 2022-08-12 南京理工大学 Protein folding identification method based on depth measurement learning
WO2023122567A1 (en) * 2021-12-21 2023-06-29 Absci Corporation Fola selection assay to identify strains with increased soluble target protein expression

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5420228A (en) * 1991-04-05 1995-05-30 University Of Pittsburgh Of The Commonwealth System Of Higher Education Fluorescent-labelled bleomycin analogues
US6027900A (en) * 1996-04-12 2000-02-22 Carnegie Institution Of Washington Methods and tools for transformation of eukaryotic algae
WO2002027327A2 (en) * 2000-08-17 2002-04-04 Sense Proteomic Limited Rapid profiling of the interactions between a chemical entity and proteins in a given proteome

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59225064A (en) * 1983-06-03 1984-12-18 ユニチカ株式会社 Carrier for immobilizing physiologically active substance
US4705616A (en) * 1986-09-15 1987-11-10 Sepragen Corporation Electrophoresis-mass spectrometry probe
FR2706678B1 (en) * 1993-06-10 1995-07-13 Commissariat Energie Atomique Mass spectrometry probe, in particular in magnetized plasma.
US20030022151A1 (en) * 2001-01-17 2003-01-30 Gopal Thinakaran Functional screening

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5420228A (en) * 1991-04-05 1995-05-30 University Of Pittsburgh Of The Commonwealth System Of Higher Education Fluorescent-labelled bleomycin analogues
US6027900A (en) * 1996-04-12 2000-02-22 Carnegie Institution Of Washington Methods and tools for transformation of eukaryotic algae
WO2002027327A2 (en) * 2000-08-17 2002-04-04 Sense Proteomic Limited Rapid profiling of the interactions between a chemical entity and proteins in a given proteome

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BENNETT R P ET AL: "Fusion of green fluorescent protein with the Zeocin-resistance marker allows visual screening and drug selection of transfected eukaryotic cells", BIOTECHNIQUES, EATON PUBLISHING, NATICK, US, vol. 24, no. 3, March 1998 (1998-03-01), pages 478 - 482, XP002114401, ISSN: 0736-6205 *
CALMELS THIERRY P G ET AL: "Nuclear localization of bacterial Streptoalloteichus hindustanus bleomycin resistance protein in mammalian cells", MOLECULAR PHARMACOLOGY, vol. 44, no. 6, 1993, pages 1135 - 1141, XP008030691, ISSN: 0026-895X *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8846320B2 (en) 2006-11-01 2014-09-30 Ventana Medical Systems, Inc. Haptens, hapten conjugates, compositions thereof and method for their preparation and use

Also Published As

Publication number Publication date
WO2004046730A2 (en) 2004-06-03
ATE368861T1 (en) 2007-08-15
AU2003302133A1 (en) 2004-06-15
US20060194715A1 (en) 2006-08-31
EP1565754A2 (en) 2005-08-24
EP1565754B1 (en) 2007-08-01
JP2010156715A (en) 2010-07-15
DK1565754T3 (en) 2007-12-17
ES2290556T3 (en) 2008-02-16
DE60315343T2 (en) 2008-04-17
DE60315343D1 (en) 2007-09-13
JP2006506447A (en) 2006-02-23
AU2003302133A8 (en) 2004-06-15

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