WO2004035757A3 - Protein synthesis using modified ribosomes - Google Patents

Protein synthesis using modified ribosomes Download PDF

Info

Publication number
WO2004035757A3
WO2004035757A3 PCT/US2003/033108 US0333108W WO2004035757A3 WO 2004035757 A3 WO2004035757 A3 WO 2004035757A3 US 0333108 W US0333108 W US 0333108W WO 2004035757 A3 WO2004035757 A3 WO 2004035757A3
Authority
WO
WIPO (PCT)
Prior art keywords
ribosomes
modified
protein synthesis
modified ribosomes
amino acids
Prior art date
Application number
PCT/US2003/033108
Other languages
French (fr)
Other versions
WO2004035757A2 (en
Inventor
Sidney M Hecht
Larisa Dedkova
Serguei Di Golovine
Original Assignee
Univ Virginia
Sidney M Hecht
Larisa Dedkova
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Virginia, Sidney M Hecht, Larisa Dedkova filed Critical Univ Virginia
Priority to AU2003286481A priority Critical patent/AU2003286481A1/en
Publication of WO2004035757A2 publication Critical patent/WO2004035757A2/en
Publication of WO2004035757A3 publication Critical patent/WO2004035757A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The present invention is directed to modified ribosomes and methods of using such modified ribosomes to synthesize proteins that contain an unusual amino acid. In particular, the modified ribosomes show an enhanced facility relative to wild type ribosomes for incorporating D-amino acids and other modified amino acids into a protein's primary sequence.
PCT/US2003/033108 2002-10-17 2003-10-17 Protein synthesis using modified ribosomes WO2004035757A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003286481A AU2003286481A1 (en) 2002-10-17 2003-10-17 Protein synthesis using modified ribosomes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US41929802P 2002-10-17 2002-10-17
US60/419,298 2002-10-17

Publications (2)

Publication Number Publication Date
WO2004035757A2 WO2004035757A2 (en) 2004-04-29
WO2004035757A3 true WO2004035757A3 (en) 2004-10-07

Family

ID=32108062

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/033108 WO2004035757A2 (en) 2002-10-17 2003-10-17 Protein synthesis using modified ribosomes

Country Status (2)

Country Link
AU (1) AU2003286481A1 (en)
WO (1) WO2004035757A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1766039A4 (en) * 2004-06-09 2011-08-24 Alexander Sunguroff Methods of making nanotechnological and macromolecular biomimetic structures
US20200181670A1 (en) * 2017-08-11 2020-06-11 Synvitrobio, Inc. Improved In Vitro Transcription/Translation (TXTL) System and Use Thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6358713B1 (en) * 1999-04-12 2002-03-19 Johns Hopkins University In vitro ribosome evolution

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6358713B1 (en) * 1999-04-12 2002-03-19 Johns Hopkins University In vitro ribosome evolution

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
DEDKOVA L. ET AL: "Enhanced D-amino acid incorporation into protein by modified ribosomes", J. AM. CHEM. SOC., vol. 125, 2003, pages 6616 - 6617, XP002979559 *
GREEN ET AL: "Ribosomes and translation", ANNUAL REV. BIOCHEM., vol. 66, 1997, pages 679 - 716, XP002979570 *
KIRSHENBAUM K. ET AL: "Biosynthesis of proteins incorporating a versatile set of phenylalanine analogues", CHEM BIO CHEM, no. 02-03, 2002, pages 235 - 237, XP002979562 *
NITTA I. ET AL: "Reconstitution of peptide bond formation with Escherichia coli 23S ribosomal RNA domains", SCIENCE, vol. 281, 31 July 1998 (1998-07-31), pages 666 - 669, XP002979561 *
O'CONNOR M. ET AL: "Mutagenesis of the peptidyltransferase center of 23S rRNA: the invariant U2449 is dispensable", NUCLEIC ACIDS RESEARCH, vol. 29, no. 3, 2001, pages 710 - 715, XP002979560 *
SHIMIZU Y. ET AL: "Cell-free translation reconstituted with purified components", NATURE BIOTECHNOLOGY, vol. 19, August 2001 (2001-08-01), pages 751 - 755, XP001060378 *

Also Published As

Publication number Publication date
WO2004035757A2 (en) 2004-04-29
AU2003286481A8 (en) 2004-05-04
AU2003286481A1 (en) 2004-05-04

Similar Documents

Publication Publication Date Title
WO2004058946A3 (en) Protein arrays
WO1998020133A3 (en) Proteins with enhanced levels of essential amino acids
GB0126194D0 (en) Pharmaco-dietary preparation having a nutrition-supplementing and nutrition-enhancing effect
EP2360176A3 (en) Hybrid and tandem expression of neisserial derived proteins
WO1997028247A3 (en) AMINO ACID-ENRICHED PLANT PROTEIN RESERVES, PARTICULARLY LYSINE-ENRICHED MAIZE η-ZEIN, AND PLANTS EXPRESSING SUCH PROTEINS
AU2002360331A1 (en) Egviii endoglucanase and nucleic acids encoding the same
WO2002038592A3 (en) Peptides, the production and use thereof for binding immunoglobulins
WO1999005270A3 (en) Crustacean antimicrobial peptides, called penaiedines
EP0794253A3 (en) Aminopeptidase GX, and a method of hydrolyzing a protein with the same
WO2004035757A3 (en) Protein synthesis using modified ribosomes
WO1995013386A3 (en) Tyrosinase-activator protein fusion enzyme
AU9625498A (en) Organ specific nutrition
WO2004007661A3 (en) Carboxy protection strategies for acidic c-terminal amino acids in chemical ligation of oligopeptides
AU2003264535A1 (en) Tumor antigen protein and utilization thereof
WO2003072733A3 (en) Modified bace
WO2002062730A3 (en) Thermodynamic propensities of amino acids in the native state ensemble: implications for fold recognition
WO2004035733A3 (en) Isolated human hepsin/46 proteins, nucleic acid molecules encoding human hepsin/46 proteins, and uses thereof
EP0851872A3 (en) Urogenital carcinoma tlp complex peptides and antibodies thereof
PT959895E (en) PEPTIDES THAT RESIST ANTIBODIES AGAINST A CONSENSUS PEOPLE OF THE CS4-CFA / I PROTEIN FAMILY
WO2003018806A1 (en) Novel human bmcc1 gene
WO2004031215A3 (en) Method for synthesizing peptides comprising at least one glycine molecule
WO2001025430A3 (en) Novel human membrane proteins
WO2004013167A3 (en) Purified polypeptides from enterococcus faecalis
WO2002016435A3 (en) Human 7tm proteins and polynucleotides encoding the same
WO2003083099A3 (en) Purified polypeptides involved in protein synthesis and modification

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE EG ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
122 Ep: pct application non-entry in european phase
NENP Non-entry into the national phase

Ref country code: JP

WWW Wipo information: withdrawn in national office

Country of ref document: JP