WO2004011636A3 - Method of enzymatic deubiquitination - Google Patents

Method of enzymatic deubiquitination Download PDF

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Publication number
WO2004011636A3
WO2004011636A3 PCT/GB2003/003320 GB0303320W WO2004011636A3 WO 2004011636 A3 WO2004011636 A3 WO 2004011636A3 GB 0303320 W GB0303320 W GB 0303320W WO 2004011636 A3 WO2004011636 A3 WO 2004011636A3
Authority
WO
WIPO (PCT)
Prior art keywords
deubiquitination
ubiquitinated
molecule
enzymatic
enzyme
Prior art date
Application number
PCT/GB2003/003320
Other languages
French (fr)
Other versions
WO2004011636A2 (en
Inventor
Paul Charles Evans
Peter John Kilshaw
Original Assignee
Babraham Inst
Paul Charles Evans
Peter John Kilshaw
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0306843A external-priority patent/GB0306843D0/en
Application filed by Babraham Inst, Paul Charles Evans, Peter John Kilshaw filed Critical Babraham Inst
Priority to AU2003251363A priority Critical patent/AU2003251363A1/en
Publication of WO2004011636A2 publication Critical patent/WO2004011636A2/en
Publication of WO2004011636A3 publication Critical patent/WO2004011636A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The invention concerns a new class of deubiquitinating enzymes and methods of deubiquitination using these enzymes or functional fragments or derivates thereof. In one aspect, the invention covers a method for enzymatic deubiquitination of a molecule which has been ubiquitinated to form a ubiquitinated molecule, comprising the step of contacting the ubiquitinated molecule with an enzyme, wherein the enzyme or a domain thereof has at least 20% amino acid sequence identity with the TRAFB domain defined by Cezanne amino acid residues 160-416 (SEQ ID NO:1).
PCT/GB2003/003320 2002-07-31 2003-07-31 Method of enzymatic deubiquitination WO2004011636A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003251363A AU2003251363A1 (en) 2002-07-31 2003-07-31 Method of enzymatic deubiquitination

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US39943902P 2002-07-31 2002-07-31
GB60/399,439 2002-07-31
GB0306843A GB0306843D0 (en) 2003-03-25 2003-03-25 Method of deubiquitination
GB0306843.4 2003-03-25

Publications (2)

Publication Number Publication Date
WO2004011636A2 WO2004011636A2 (en) 2004-02-05
WO2004011636A3 true WO2004011636A3 (en) 2004-07-01

Family

ID=31189618

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2003/003320 WO2004011636A2 (en) 2002-07-31 2003-07-31 Method of enzymatic deubiquitination

Country Status (2)

Country Link
AU (1) AU2003251363A1 (en)
WO (1) WO2004011636A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0610542D0 (en) 2006-05-26 2006-07-05 Medical Res Council Screening method

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001032696A2 (en) * 1999-11-05 2001-05-10 The Burnham Institute Traf family proteins
WO2003091411A2 (en) * 2002-04-25 2003-11-06 President And Fellows Of Harvard College Semisynthetic protein-based site-directed probes for identification and inhibition of active sites, and methods therefor

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001032696A2 (en) * 1999-11-05 2001-05-10 The Burnham Institute Traf family proteins
WO2003091411A2 (en) * 2002-04-25 2003-11-06 President And Fellows Of Harvard College Semisynthetic protein-based site-directed probes for identification and inhibition of active sites, and methods therefor

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
BORODOVSKY, A. ET AL.: "Chemistry-Based Functional Proteomics Reveals Novel Members of the Deubiquitinating Enzyme Family", CHEMISTRY & BIOLOGY, vol. 9, no. 10, October 2002 (2002-10-01), pages 1149 - 1159, XP008027097 *
DATABASE NCBI [online] 22 May 2002 (2002-05-22), XP002269111, Database accession no. AAC19188 *
DATABASE NCBI [online] 24 January 2002 (2002-01-24), XP002269110, Database accession no. CAA98471 *
DATABASE NCBI [online] 8 February 2002 (2002-02-08), EVANS, P.C. ET AL.: "Cezanne 2 protein [Homo sapiens]", XP002269112, Database accession no. CAD23047 *
EVANS, P.C. ET AL.: "A Novel Type of Deubiquinating Enzyme", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 278, no. 25, 20 June 2003 (2003-06-20), pages 23180 - 23186, XP002269109 *
EVANS, P.C. ET AL.: "Isolation and characterization of two novel A20-like proteins", BIOCHEMICAL JOURNAL, vol. 357, no. 3, 1 August 2001 (2001-08-01), pages 617 - 623, XP002269108 *
MAKAROVA, K.S. ET AL.: "A novel superfamily of predicted cysteine proteases from eukaryotes, viruses and Chlamydia pneumoniae", TIBS TRENDS IN BIOCHEMICAL SCIENCES, vol. 25, no. 2, AAC19188, February 2000 (2000-02-01), pages 50 - 52, XP004189093 *
WILKINSON, K.D.: "Ubiquitination and deubiquitination: Targeting of proteins for degradation by the proteasome", SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, vol. 11, no. 3, June 2000 (2000-06-01), pages 141 - 148, XP002269261 *

Also Published As

Publication number Publication date
AU2003251363A8 (en) 2004-02-16
WO2004011636A2 (en) 2004-02-05
AU2003251363A1 (en) 2004-02-16

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