WO2003050265A3 - Compositions and methods for normalizing assays - Google Patents

Compositions and methods for normalizing assays Download PDF

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Publication number
WO2003050265A3
WO2003050265A3 PCT/US2002/039671 US0239671W WO03050265A3 WO 2003050265 A3 WO2003050265 A3 WO 2003050265A3 US 0239671 W US0239671 W US 0239671W WO 03050265 A3 WO03050265 A3 WO 03050265A3
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WO
Grant status
Application
Patent type
Prior art keywords
methods
assays
normalizing
compositions
provided
Prior art date
Application number
PCT/US2002/039671
Other languages
French (fr)
Other versions
WO2003050265A2 (en )
Inventor
Justin Stege
Lori Preston
David Weiner
Original Assignee
Diversa Corp
Justin Stege
Lori Preston
David Weiner
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62DNA sequences coding for fusion proteins
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00Genetically modified animals
    • A01K2217/05Animals comprising random inserted nucleic acids (transgenic)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/20Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/60Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/70Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/90Fusion polypeptide containing a motif for post-translational modification
    • C07K2319/92Fusion polypeptide containing a motif for post-translational modification containing an intein ("protein splicing")domain

Abstract

In one aspect, the invention provides compositions and methods for expression normalization of enzyme activity screens. Also provided are methods for method for normalizing gene expression in assays and high throughput enzymatic screens to measure enzyme activity. Also provided are novel inteins and nucleic acids encoding them.
PCT/US2002/039671 2001-12-10 2002-12-10 Compositions and methods for normalizing assays WO2003050265A3 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US33983701 true 2001-12-10 2001-12-10
US60/339,837 2001-12-10

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
CA 2472054 CA2472054A1 (en) 2001-12-10 2002-12-10 Compositions and methods for normalizing assays
JP2003551287A JP2005512526A (en) 2001-12-10 2002-12-10 Composition and method for standardizing the quantitative
EP20020795831 EP1453952A2 (en) 2001-12-10 2002-12-10 Compositions and methods for normalizing assays
AU2002360565A AU2002360565A1 (en) 2001-12-10 2002-12-10 Compositions and methods for normalizing assays

Publications (2)

Publication Number Publication Date
WO2003050265A2 true WO2003050265A2 (en) 2003-06-19
WO2003050265A3 true true WO2003050265A3 (en) 2004-02-19

Family

ID=23330827

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/039671 WO2003050265A3 (en) 2001-12-10 2002-12-10 Compositions and methods for normalizing assays

Country Status (4)

Country Link
EP (1) EP1453952A2 (en)
JP (1) JP2005512526A (en)
CA (1) CA2472054A1 (en)
WO (1) WO2003050265A3 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102712681B (en) * 2009-11-06 2016-07-06 谷万达公司 Intein modified enzymes and industrial use and preparation

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9464333B2 (en) 2009-11-06 2016-10-11 Agrivida, Inc. Intein-modified enzymes, their production and industrial applications
US9598700B2 (en) 2010-06-25 2017-03-21 Agrivida, Inc. Methods and compositions for processing biomass with elevated levels of starch
CN103547659B (en) 2011-03-07 2016-09-28 谷万达公司 Pretreatment expression and plant cell wall degrading enzymes hydrolyzing biomass

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020177691A1 (en) * 2001-03-20 2002-11-28 Scott Charles P. Trans inteins for protein domain shuffling and biopolymerization
US6562576B2 (en) * 2001-01-04 2003-05-13 Myriad Genetics, Inc. Yeast two-hybrid system and use thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6562576B2 (en) * 2001-01-04 2003-05-13 Myriad Genetics, Inc. Yeast two-hybrid system and use thereof
US20020177691A1 (en) * 2001-03-20 2002-11-28 Scott Charles P. Trans inteins for protein domain shuffling and biopolymerization

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102712681B (en) * 2009-11-06 2016-07-06 谷万达公司 Intein modified enzymes and industrial use and preparation
CN102712682B (en) * 2009-11-06 2016-07-06 谷万达公司 Intein modified enzymes and industrial use and preparation

Also Published As

Publication number Publication date Type
JP2005512526A (en) 2005-05-12 application
EP1453952A2 (en) 2004-09-08 application
WO2003050265A2 (en) 2003-06-19 application
CA2472054A1 (en) 2003-06-19 application

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