WO2004009837A3 - A high resolution typing system for pathogenic mycobacterium tuberculosum - Google Patents

A high resolution typing system for pathogenic mycobacterium tuberculosum Download PDF

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Publication number
WO2004009837A3
WO2004009837A3 PCT/US2003/022950 US0322950W WO2004009837A3 WO 2004009837 A3 WO2004009837 A3 WO 2004009837A3 US 0322950 W US0322950 W US 0322950W WO 2004009837 A3 WO2004009837 A3 WO 2004009837A3
Authority
WO
WIPO (PCT)
Prior art keywords
tuberculosum
mycobacterium tuberculosum
high resolution
typing system
pathogenic mycobacterium
Prior art date
Application number
PCT/US2003/022950
Other languages
French (fr)
Other versions
WO2004009837A2 (en
Inventor
Paul S Keim
James M Schupp
Robert Scott Spurgiesz
Original Assignee
Univ Arizona
Paul S Keim
James M Schupp
Robert Scott Spurgiesz
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Arizona, Paul S Keim, James M Schupp, Robert Scott Spurgiesz filed Critical Univ Arizona
Priority to EP03765949A priority Critical patent/EP1573038A2/en
Priority to AU2003254128A priority patent/AU2003254128A1/en
Publication of WO2004009837A2 publication Critical patent/WO2004009837A2/en
Publication of WO2004009837A3 publication Critical patent/WO2004009837A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

MLVA methods for strain discrimination among Mycobacterium tuberculosum strains are disclosed. Nine VNTR loci have been identified from genomic sequences of Mycobacterium tuberculosum strains and primer pairs suitable for amplifying the VNTR by PCR are disclosed. Polymorphisms at these loci were used to resolve genotypes into distinct groups. This sub-typing scheme is useful for the epidemiological study of Mycobacterium tuberculosum and may be applied to the local detection of the pathological causative agent of tuberculosum.
PCT/US2003/022950 2002-07-19 2003-07-21 A high resolution typing system for pathogenic mycobacterium tuberculosum WO2004009837A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP03765949A EP1573038A2 (en) 2002-07-19 2003-07-21 A high resolution typing system for pathogenic mycobacterium tuberculosum
AU2003254128A AU2003254128A1 (en) 2002-07-19 2003-07-21 A high resolution typing system for pathogenic mycobacterium tuberculosum

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US39722402P 2002-07-19 2002-07-19
US60/397,224 2002-07-19

Publications (2)

Publication Number Publication Date
WO2004009837A2 WO2004009837A2 (en) 2004-01-29
WO2004009837A3 true WO2004009837A3 (en) 2007-03-22

Family

ID=30771019

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/022950 WO2004009837A2 (en) 2002-07-19 2003-07-21 A high resolution typing system for pathogenic mycobacterium tuberculosum

Country Status (4)

Country Link
US (2) US7026467B2 (en)
EP (1) EP1573038A2 (en)
AU (1) AU2003254128A1 (en)
WO (1) WO2004009837A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1789577A4 (en) * 2004-07-12 2010-11-17 Avestha Gengraine Tech Pvt Ltd Construction of a comparative database and identificaiton of virulence factors through comparison of polymorphic regions in clinical isolates of infectious organisms
GB0806431D0 (en) 2008-04-09 2008-05-14 Health Prot Agency Rapid detection of mycobacteria
EP2929054A4 (en) * 2012-12-04 2016-07-27 Boston Medical Ct Corp Primers, probes and methods for mycobacterium tuberculosis specific diagnosis
CN112501321B (en) * 2020-11-19 2022-07-15 厦门大学 Method for molecular typing of mycobacterium tuberculosis
WO2023077482A1 (en) * 2021-11-06 2023-05-11 江汉大学 Combination of mnp markers of mycobacterium tuberculosis, primer pair combination, kit, and uses of combination, primer pair combination and kit

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997044463A2 (en) * 1996-05-17 1997-11-27 Institut Pasteur De Lille Identification and cloning of a mycobacterial antigen corresponding to a heparin-binding haemagglutinin
WO1999009186A2 (en) * 1997-08-14 1999-02-25 Institut Pasteur Polypeptide nucleic sequences exported from mycobacteria, vectors comprising same and uses for diagnosing and preventing tuberculosis
WO2001002568A2 (en) * 1999-07-02 2001-01-11 Chiron Corporation Human genes and gene expression products
US6228371B1 (en) * 1995-06-15 2001-05-08 University Of Victoria Innovation And Development Corp. Mycobacterium tuberculosis DNA sequences encoding immunostimulatory peptides
WO2001035317A1 (en) * 1999-11-12 2001-05-17 The Regents Of The University Of California Determining the functions and interactions of proteins by comparative analysis
US6294328B1 (en) * 1998-06-24 2001-09-25 The Institute For Genomic Research DNA sequences for strain analysis in Mycobacterium tuberculosis

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US6479235B1 (en) 1994-09-30 2002-11-12 Promega Corporation Multiplex amplification of short tandem repeat loci
US6449562B1 (en) 1996-10-10 2002-09-10 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and method
US6270973B1 (en) 1998-03-13 2001-08-07 Promega Corporation Multiplex method for nucleic acid detection

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6228371B1 (en) * 1995-06-15 2001-05-08 University Of Victoria Innovation And Development Corp. Mycobacterium tuberculosis DNA sequences encoding immunostimulatory peptides
WO1997044463A2 (en) * 1996-05-17 1997-11-27 Institut Pasteur De Lille Identification and cloning of a mycobacterial antigen corresponding to a heparin-binding haemagglutinin
WO1999009186A2 (en) * 1997-08-14 1999-02-25 Institut Pasteur Polypeptide nucleic sequences exported from mycobacteria, vectors comprising same and uses for diagnosing and preventing tuberculosis
US6294328B1 (en) * 1998-06-24 2001-09-25 The Institute For Genomic Research DNA sequences for strain analysis in Mycobacterium tuberculosis
WO2001002568A2 (en) * 1999-07-02 2001-01-11 Chiron Corporation Human genes and gene expression products
WO2001035317A1 (en) * 1999-11-12 2001-05-17 The Regents Of The University Of California Determining the functions and interactions of proteins by comparative analysis

Also Published As

Publication number Publication date
WO2004009837A2 (en) 2004-01-29
US7592135B2 (en) 2009-09-22
US20040121366A1 (en) 2004-06-24
AU2003254128A1 (en) 2004-02-09
US7026467B2 (en) 2006-04-11
AU2003254128A8 (en) 2004-02-09
EP1573038A2 (en) 2005-09-14
US20050266492A1 (en) 2005-12-01

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