WO2004005479A3 - A high resolution typing system for pathogenic borrelia - Google Patents

A high resolution typing system for pathogenic borrelia Download PDF

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Publication number
WO2004005479A3
WO2004005479A3 PCT/US2003/021153 US0321153W WO2004005479A3 WO 2004005479 A3 WO2004005479 A3 WO 2004005479A3 US 0321153 W US0321153 W US 0321153W WO 2004005479 A3 WO2004005479 A3 WO 2004005479A3
Authority
WO
WIPO (PCT)
Prior art keywords
borrelia
high resolution
disclosed
vntr
loci
Prior art date
Application number
PCT/US2003/021153
Other languages
French (fr)
Other versions
WO2004005479A2 (en
Inventor
Paul S Keim
Jason Farlow
Original Assignee
Univ Arizona
Paul S Keim
Jason Farlow
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Arizona, Paul S Keim, Jason Farlow filed Critical Univ Arizona
Priority to EP03763263A priority Critical patent/EP1539782A4/en
Priority to AU2003253805A priority patent/AU2003253805A1/en
Publication of WO2004005479A2 publication Critical patent/WO2004005479A2/en
Publication of WO2004005479A3 publication Critical patent/WO2004005479A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Abstract

MLVA methods for strain discrimination among globally diverse Borrelia isolates including B. burgdorferi, B. afzelii, and B. garinii are disclosed. Ten VNTR loci have been identified from genomic and plasmid sequences of Borrelia strains and primer pairs suitable for amplifying the VNTR by PCR are disclosed. Polymorphisms at these loci were used to resolve genotypes into distinct groups. The resolution of 30 unique genotypes into five to seven distinct groups is demonstrated.. This sub-typing scheme is useful for the epidemiological study of Borrelia and may be applied to the local detection of the pathological causative agent of Lyme Disease.
PCT/US2003/021153 2002-07-02 2003-07-02 A high resolution typing system for pathogenic borrelia WO2004005479A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP03763263A EP1539782A4 (en) 2002-07-02 2003-07-02 A high resolution typing system for pathogenic borrelia
AU2003253805A AU2003253805A1 (en) 2002-07-02 2003-07-02 A high resolution typing system for pathogenic borrelia

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US39349702P 2002-07-02 2002-07-02
US60/393,497 2002-07-02

Publications (2)

Publication Number Publication Date
WO2004005479A2 WO2004005479A2 (en) 2004-01-15
WO2004005479A3 true WO2004005479A3 (en) 2004-12-23

Family

ID=30115590

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/021153 WO2004005479A2 (en) 2002-07-02 2003-07-02 A high resolution typing system for pathogenic borrelia

Country Status (3)

Country Link
EP (1) EP1539782A4 (en)
AU (1) AU2003253805A1 (en)
WO (1) WO2004005479A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2016118766A2 (en) * 2015-01-21 2016-07-28 T2 Biosystems, Inc. Nmr methods and systems for the rapid detection of tick-borne pathogens

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998058943A1 (en) * 1997-06-20 1998-12-30 Human Genome Sciences, Inc. Borrelia burgdorferi polynucleotides and sequences

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998058943A1 (en) * 1997-06-20 1998-12-30 Human Genome Sciences, Inc. Borrelia burgdorferi polynucleotides and sequences

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP1539782A4 *

Also Published As

Publication number Publication date
AU2003253805A8 (en) 2004-01-23
EP1539782A2 (en) 2005-06-15
EP1539782A4 (en) 2005-11-16
WO2004005479A2 (en) 2004-01-15
AU2003253805A1 (en) 2004-01-23

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