WO2004003230A3 - Diagnostic method for identifying the biological age of a dna sample - Google Patents

Diagnostic method for identifying the biological age of a dna sample Download PDF

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Publication number
WO2004003230A3
WO2004003230A3 PCT/GB2003/002759 GB0302759W WO2004003230A3 WO 2004003230 A3 WO2004003230 A3 WO 2004003230A3 GB 0302759 W GB0302759 W GB 0302759W WO 2004003230 A3 WO2004003230 A3 WO 2004003230A3
Authority
WO
WIPO (PCT)
Prior art keywords
age
dna
identifying
biological age
dna sample
Prior art date
Application number
PCT/GB2003/002759
Other languages
French (fr)
Other versions
WO2004003230A2 (en
Inventor
Maj Anita Hulten
Original Assignee
Simeg Ltd
Maj Anita Hulten
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Simeg Ltd, Maj Anita Hulten filed Critical Simeg Ltd
Priority to AU2003246911A priority Critical patent/AU2003246911A1/en
Publication of WO2004003230A2 publication Critical patent/WO2004003230A2/en
Publication of WO2004003230A3 publication Critical patent/WO2004003230A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A method for identifying the biological age of a DNA sample from a subject, said method comprising subjecting the sample to exonucleolytic digestion by an enzyme so as to partially remove end regions of said DNA, and detecting the presence of a DNA sequence normally found at the terminal region of the DNA. Determination of biological age may be useful for comparison with chronological age in diverse situations including predisposition to malignancy, in research into ageing, in the determination of particular therapies or remedies for age related conditions including those of old age, or in forensics.
PCT/GB2003/002759 2002-06-27 2003-06-26 Diagnostic method for identifying the biological age of a dna sample WO2004003230A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003246911A AU2003246911A1 (en) 2002-06-27 2003-06-26 Diagnostic method for identifying the biological age of a dna sample

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0214842.7A GB0214842D0 (en) 2002-06-27 2002-06-27 Diagnostic method
GB0214842.7 2002-06-27

Publications (2)

Publication Number Publication Date
WO2004003230A2 WO2004003230A2 (en) 2004-01-08
WO2004003230A3 true WO2004003230A3 (en) 2004-04-01

Family

ID=9939373

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2003/002759 WO2004003230A2 (en) 2002-06-27 2003-06-26 Diagnostic method for identifying the biological age of a dna sample

Country Status (3)

Country Link
AU (1) AU2003246911A1 (en)
GB (1) GB0214842D0 (en)
WO (1) WO2004003230A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2649456C1 (en) * 2017-03-31 2018-04-03 Чингис Куанычевич Мустафин Method for predicting the rates of aging and evaluating the effectiveness of health programs

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5834193A (en) * 1995-06-07 1998-11-10 Geron Corporation Methods for measuring telomere length
WO2001081626A2 (en) * 2000-04-20 2001-11-01 Simeg Limited Methods for clinical diagnosis
WO2002004672A2 (en) * 2000-07-10 2002-01-17 Simeg Limited Diagnostic method for the identification of foetal dna in a maternal sample
US20030104462A1 (en) * 2001-12-05 2003-06-05 Peter Lahnert Method for determining telomer length and its use for assessing age

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5834193A (en) * 1995-06-07 1998-11-10 Geron Corporation Methods for measuring telomere length
WO2001081626A2 (en) * 2000-04-20 2001-11-01 Simeg Limited Methods for clinical diagnosis
WO2002004672A2 (en) * 2000-07-10 2002-01-17 Simeg Limited Diagnostic method for the identification of foetal dna in a maternal sample
US20030104462A1 (en) * 2001-12-05 2003-06-05 Peter Lahnert Method for determining telomer length and its use for assessing age

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
COUNTER C M: "The roles of telomeres and telomerase in cell life span.", MUTATION RESEARCH. NETHERLANDS OCT 1996, vol. 366, no. 1, October 1996 (1996-10-01), pages 45 - 63, XP009023248, ISSN: 0027-5107 *
IWAMA HIROSHI ET AL: "Telomeric length and telomerase activity vary with age in peripheral blood cells obtained from normal individuals", HUMAN GENETICS, BERLIN, DE, vol. 102, no. 4, April 1998 (1998-04-01), pages 397 - 402, XP002200863 *
KANG MO K ET AL: "The telomeric length and heterogeneity decrease with age in normal human oral keratinocytes.", MECHANISMS OF AGEING AND DEVELOPMENT. IRELAND 31 MAR 2002, vol. 123, no. 6, 31 March 2002 (2002-03-31), pages 585 - 592, XP002265376, ISSN: 0047-6374 *
LANSDORP P M ET AL: "HETEROGENEITY IN TELOMERE LENGTH OF HUMAN CHROMOSOMES", HUMAN MOLECULAR GENETICS, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 5, no. 5, 1996, pages 685 - 691, XP000601662, ISSN: 0964-6906 *
LINDSEY J ET AL: "IN-VIVO LOSS OF TELOMERIC REPEATS WITH AGE IN HUMANS", MUTATION RESEARCH, vol. 256, no. 1, 1991, pages 45 - 48, XP009023247, ISSN: 0027-5107 *
MATSUNAGA H ET AL: "Beta-galactosidase histochemistry and telomere loss in senescent retinal pigment epithelial cells.", INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE. UNITED STATES JAN 1999, vol. 40, no. 1, January 1999 (1999-01-01), pages 197 - 202, XP009023252, ISSN: 0146-0404 *
MOYZIS R K ET AL: "A HIGHLY CONSERVED REPETITIVE DNA SEQUENCE, (TTAGGG)N, PRESENT AT THE TELOMERES OF HUMAN CHROMOSOMES", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 85, 1 September 1988 (1988-09-01), pages 6622 - 6626, XP002030776, ISSN: 0027-8424 *
VAZIRI H ET AL: "Loss of telomeric DNA during aging of normal and trisomy 21 human lymphocytes.", AMERICAN JOURNAL OF HUMAN GENETICS. UNITED STATES APR 1993, vol. 52, no. 4, April 1993 (1993-04-01), pages 661 - 667, XP009023249, ISSN: 0002-9297 *

Also Published As

Publication number Publication date
AU2003246911A8 (en) 2004-01-19
WO2004003230A2 (en) 2004-01-08
AU2003246911A1 (en) 2004-01-19
GB0214842D0 (en) 2002-08-07

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