WO2003106666A3 - Protein c variants with altered properties - Google Patents

Protein c variants with altered properties Download PDF

Info

Publication number
WO2003106666A3
WO2003106666A3 PCT/DK2003/000392 DK0300392W WO03106666A3 WO 2003106666 A3 WO2003106666 A3 WO 2003106666A3 DK 0300392 W DK0300392 W DK 0300392W WO 03106666 A3 WO03106666 A3 WO 03106666A3
Authority
WO
WIPO (PCT)
Prior art keywords
protein
variants
altered properties
treatment
residue
Prior art date
Application number
PCT/DK2003/000392
Other languages
French (fr)
Other versions
WO2003106666A2 (en
Inventor
Bobby Soni
Original Assignee
Maxygen Aps
Maxygen Holdings Ltd
Bobby Soni
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Maxygen Aps, Maxygen Holdings Ltd, Bobby Soni filed Critical Maxygen Aps
Priority to AU2003232176A priority Critical patent/AU2003232176A1/en
Publication of WO2003106666A2 publication Critical patent/WO2003106666A2/en
Publication of WO2003106666A3 publication Critical patent/WO2003106666A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6464—Protein C (3.4.21.69)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/48—Hydrolases (3) acting on peptide bonds (3.4)
    • A61K38/482—Serine endopeptidases (3.4.21)
    • A61K38/4866—Protein C (3.4.21.69)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)

Abstract

The invention relates to the use of a human protein C variant comprising at least one amino acid modification in position 306-314, e.g. substitution of a charged residue with an uncharged residue, for the manufacture of a medicament for the treatment of a hypercoagulable state or acquired protein C deficiency, e.g. for the treatment of sepsis. The variants have a reduced anticoagulant activity compared to non-modified human activated protein C.
PCT/DK2003/000392 2002-06-14 2003-06-13 Protein c variants with altered properties WO2003106666A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003232176A AU2003232176A1 (en) 2002-06-14 2003-06-13 Protein c variants with altered properties

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US38889202P 2002-06-14 2002-06-14
US60/388,892 2002-06-14

Publications (2)

Publication Number Publication Date
WO2003106666A2 WO2003106666A2 (en) 2003-12-24
WO2003106666A3 true WO2003106666A3 (en) 2007-11-15

Family

ID=29736559

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK2003/000392 WO2003106666A2 (en) 2002-06-14 2003-06-13 Protein c variants with altered properties

Country Status (2)

Country Link
AU (1) AU2003232176A1 (en)
WO (1) WO2003106666A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004113385A1 (en) * 2003-06-20 2004-12-29 Maxygen Holdings Ltd. Protein c propeptide variants
WO2006044294A2 (en) * 2004-10-14 2006-04-27 Eli Lilly And Company Human protein c analogs

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5453373A (en) * 1992-05-21 1995-09-26 Eli Lilly And Company Protein C derivatives
WO1998044000A1 (en) * 1997-04-03 1998-10-08 T.A.C. Thrombosis And Coagulation Aktiebolag Recombinant protein c and protein s variants
WO2002032461A2 (en) * 2000-10-18 2002-04-25 Maxygen Aps Protein c or activated protein c-like molecules

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5453373A (en) * 1992-05-21 1995-09-26 Eli Lilly And Company Protein C derivatives
WO1998044000A1 (en) * 1997-04-03 1998-10-08 T.A.C. Thrombosis And Coagulation Aktiebolag Recombinant protein c and protein s variants
WO2002032461A2 (en) * 2000-10-18 2002-04-25 Maxygen Aps Protein c or activated protein c-like molecules

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
ANDREW J. GALE ET AL: "The autolysis loop of activated protein C interacts with factor Va and differentiates between the Arg506 and Arg306 cleavage sites", BLOOD, vol. 96, no. 2, July 2000 (2000-07-01), pages 585 - 593, XP002255339 *
ROLF M. MESTERS ET AL: "Interactions and inhibition of blood coagulation factor Va involving residues 311-325 of activated protein C", PROTEIN SCIENCE, vol. 2, 1993, pages 1482 - 1489, XP002255340 *

Also Published As

Publication number Publication date
WO2003106666A2 (en) 2003-12-24
AU2003232176A8 (en) 2003-12-31
AU2003232176A1 (en) 2003-12-31

Similar Documents

Publication Publication Date Title
BE2021C505I2 (en)
WO2003101401A3 (en) Use of nrg4, or inhibitors thereof, in the treatment of colon and pancreatic cancer
DE60028970D1 (en) AT HER2 BINDING PEPTIDE COMPOUNDS
WO2000078344A8 (en) Prion protein peptides and uses thereof
HK1087398A1 (en) 1-phenylalkanecarboxylic acid derivatives for the treatment of neurodegenerative diseases
YU37304A (en) Specific binding agents of human angiopoietin-2
MXPA02011656A (en) Nrg 2 nucleic acid molecules, polypeptides, and diagnostic and therapeutic methods.
HK1095357A1 (en) Factor vii or viia gla domain variants
MY136095A (en) Use of human anti-ctla-4 antibodies for treatment of cancer
EP2537524A3 (en) Treatment of T-cell mediated diseases
WO2003096990A3 (en) Protein cages for the delivery of medical imaging and therapy
WO2005082399A3 (en) Treatment of bacterial infections
IL171384A (en) Use of slurp-1 in the preparation of medicaments for the treatment of neurological disorders
WO2003048205A3 (en) Novel proteins with il-6 inhibiting activity
YU68702A (en) Novel lhrh-antagonists, production and use thereof as medicament
GB0507598D0 (en) Composition
MXPA02000680A (en) Vgf polypeptides and methods of treating vgf-related disorders.
WO2001007479A3 (en) Fragments of cellular prion protein and methods useful in the diagnosis and treatment of prion diseases
WO2003106666A3 (en) Protein c variants with altered properties
PL357999A1 (en) Lhrh-antagonists, production and use thereof as medicament
WO2004113386A3 (en) Modified hirudin proteins and t-cell epitopes in hirudin
IL174134A0 (en) Use of a protein in the preparation of a medicament for treatment of fibrotic diseases
WO2007049065A3 (en) Vwfa, collagen and kunitz domain containing protein
WO2003086301A3 (en) Antibodies that specifically bind to tr2
TW200517125A (en) Pharmaceutical composition for treatment of solid cancers

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NI NO NZ OM PH PL PT RO RU SD SE SG SI SK SL TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
122 Ep: pct application non-entry in european phase
NENP Non-entry into the national phase

Ref country code: JP

WWW Wipo information: withdrawn in national office

Country of ref document: JP