WO2003105723A3 - Mutant plant caffeic acid/5-hydroxyferulic acid 3/5-0-methyltransferases (comt), methods for preparation and use thereof - Google Patents

Mutant plant caffeic acid/5-hydroxyferulic acid 3/5-0-methyltransferases (comt), methods for preparation and use thereof Download PDF

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Publication number
WO2003105723A3
WO2003105723A3 PCT/US2003/018837 US0318837W WO03105723A3 WO 2003105723 A3 WO2003105723 A3 WO 2003105723A3 US 0318837 W US0318837 W US 0318837W WO 03105723 A3 WO03105723 A3 WO 03105723A3
Authority
WO
WIPO (PCT)
Prior art keywords
comt
acid
methods
methyltransferases
preparation
Prior art date
Application number
PCT/US2003/018837
Other languages
French (fr)
Other versions
WO2003105723A2 (en
Inventor
Joseph P Noel
Chloe Zubieta
Parvathi Kota
Richard Dixon
Original Assignee
Salk Inst For Biological Studi
Samuel Robets Noble Foundation
Joseph P Noel
Chloe Zubieta
Parvathi Kota
Richard Dixon
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Salk Inst For Biological Studi, Samuel Robets Noble Foundation, Joseph P Noel, Chloe Zubieta, Parvathi Kota, Richard Dixon filed Critical Salk Inst For Biological Studi
Priority to AU2003251522A priority Critical patent/AU2003251522A1/en
Publication of WO2003105723A2 publication Critical patent/WO2003105723A2/en
Publication of WO2003105723A3 publication Critical patent/WO2003105723A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2299/00—Coordinates from 3D structures of peptides, e.g. proteins or enzymes

Abstract

The present invention provides compositions comprising caffeic acid /5-hydroxyferulic acid 3/5-O-methyltransferase (COMT) in crystalline form. There are provided high resolution structures of COMT complexed with S-adenosyl-L-homocysteine (SAH) and ferulate and complexed with SAH and 5-hydroxyconiferaldehyde. The discovered structure of a COMT provides the first three-dimensional view of the structural basis for substrate selectivity by COMT between lignin precursors in the lignin biosynthetic pathway. The present invention provides further compositions derived from and methods of using the three-dimensional atomic coordinates disclosed herein.
PCT/US2003/018837 2002-06-14 2003-06-13 Mutant plant caffeic acid/5-hydroxyferulic acid 3/5-0-methyltransferases (comt), methods for preparation and use thereof WO2003105723A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003251522A AU2003251522A1 (en) 2002-06-14 2003-06-13 Mutant plant caffeic acid/5-hydroxyferulic acid 3/5-0-methyltransferases (comt), methods for preparation and use thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US38924102P 2002-06-14 2002-06-14
US60/389,241 2002-06-14

Publications (2)

Publication Number Publication Date
WO2003105723A2 WO2003105723A2 (en) 2003-12-24
WO2003105723A3 true WO2003105723A3 (en) 2005-09-09

Family

ID=29736616

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/018837 WO2003105723A2 (en) 2002-06-14 2003-06-13 Mutant plant caffeic acid/5-hydroxyferulic acid 3/5-0-methyltransferases (comt), methods for preparation and use thereof

Country Status (2)

Country Link
AU (1) AU2003251522A1 (en)
WO (1) WO2003105723A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2007203378B2 (en) 2000-06-14 2011-12-08 Agriculture Victoria Services Pty Ltd Modification of lignin biosynthesis (2)
US8921653B2 (en) 2001-06-14 2014-12-30 Agriculture Victoria Services Pty Ltd Modification of lignin biosynthesis
US8889392B2 (en) * 2010-10-28 2014-11-18 Brookhaven Science Associates, Llc Monolignol 4-O-methyltransferases and uses thereof
US9556420B2 (en) 2013-06-18 2017-01-31 Brookhaven Science Associates, Llc Specialized (iso)eugenol-4-O-methyltransferases (s-IEMTs) and methods of making and using the same

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5130105A (en) * 1990-10-23 1992-07-14 The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration Protein crystal growth tray assembly
WO1998035048A2 (en) * 1997-02-07 1998-08-13 Princeton University Engineered protein kinases which can utilize modified nucleotide triphosphate substrates
WO2001073090A2 (en) * 2000-03-24 2001-10-04 The Samuel Roberts Noble Foundation, Inc. Method for modifying lignin composition and increasing in vivo digestibility of forages
WO2003006987A1 (en) * 2000-12-11 2003-01-23 The Salk Institute For Biological Studies Methods and compositions for determining enzymatic activity and specificity of methyltransferases

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5130105A (en) * 1990-10-23 1992-07-14 The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration Protein crystal growth tray assembly
WO1998035048A2 (en) * 1997-02-07 1998-08-13 Princeton University Engineered protein kinases which can utilize modified nucleotide triphosphate substrates
US6390821B1 (en) * 1997-02-07 2002-05-21 Princeton University Engineered protein kinases which can utilize modified nucleotide triphosphate substrates
WO2001073090A2 (en) * 2000-03-24 2001-10-04 The Samuel Roberts Noble Foundation, Inc. Method for modifying lignin composition and increasing in vivo digestibility of forages
WO2003006987A1 (en) * 2000-12-11 2003-01-23 The Salk Institute For Biological Studies Methods and compositions for determining enzymatic activity and specificity of methyltransferases

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
DATABASE PROTEIN ON NCBI 6 February 2002 (2002-02-06), ZUBIETA C ET AL: "Structural basis for the modulation of lignin monomer methylation by caffeic acid/5-hydroxyferulic acid 3/5-O-methyltransferase", XP002997192 *
DATABASE PROTEIN ON NCBI 6 February 2002 (2002-02-06), ZUBIETA C ET AL: "Structural basis for the modulation of lignin monomer methylation by caffeic acid/5-hydroxyferulic acid 3/5-O-methyltransferase", XP002997193 *
DOUBLIE S: "Preparation of selenomethionyl protein for phase determination", METHODS IN ENZYMOL., vol. 276, 1997, pages 523 - 530, XP001053309 *
GOWRI ET AL: "Stress responses in alfalfa (medicago sativa L.)", PLANT PHYSIOL, vol. 97, 1991, pages 7 - 14, XP002989401 *
PLANT CELL, vol. 14, June 2002 (2002-06-01), pages 1265 - 1277 *
ZUBIETA C. ET AL: "Structural Basis for the Modulation of Lignin Monomer Methylation by Caffeic Acid/5-Hydroxyferulic Acid 3/5-O-Methyltransferase", CELL PLANT, vol. 14, June 2002 (2002-06-01), pages 1265 - 1277, XP002989402 *
ZUBIETA C. ET AL: "Structures of two natural product methyltransferases reveal the basis for substrate specificity in plant O-methyltransferases", NATURE STRUCTURE BIOLOGY, vol. 8, no. 3, March 2001 (2001-03-01), pages 271 - 279, XP002989403 *

Also Published As

Publication number Publication date
WO2003105723A2 (en) 2003-12-24
AU2003251522A1 (en) 2003-12-31
AU2003251522A8 (en) 2003-12-31

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