WO2003099229A3 - Functional protein expression for rapid cell-free phenotyping - Google Patents

Functional protein expression for rapid cell-free phenotyping Download PDF

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Publication number
WO2003099229A3
WO2003099229A3 PCT/US2003/016681 US0316681W WO03099229A3 WO 2003099229 A3 WO2003099229 A3 WO 2003099229A3 US 0316681 W US0316681 W US 0316681W WO 03099229 A3 WO03099229 A3 WO 03099229A3
Authority
WO
WIPO (PCT)
Prior art keywords
phenotyping
free
protein expression
functional protein
rapid cell
Prior art date
Application number
PCT/US2003/016681
Other languages
French (fr)
Other versions
WO2003099229A2 (en
Inventor
Laurence Mccarthy
Lilly Kong
Tang Shao
Xin Su
Original Assignee
Focus Technologies Inc
Laurence Mccarthy
Lilly Kong
Tang Shao
Xin Su
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Focus Technologies Inc, Laurence Mccarthy, Lilly Kong, Tang Shao, Xin Su filed Critical Focus Technologies Inc
Priority to AU2003243321A priority Critical patent/AU2003243321A1/en
Publication of WO2003099229A2 publication Critical patent/WO2003099229A2/en
Publication of WO2003099229A3 publication Critical patent/WO2003099229A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Disclosed herein are methods for assaying the phenotype of a bioactive molecule in the presence and absence of compounds that are known inhibitors of the phenotypable activity of the bioactive molecule. Also disclosed are methods for discovering compounds that can inhibit the phenotypable activity of a bioactive molecule. The methods and assays of the present invention are useful in developing and monitoring a chemotherapy regimen for a patient, to detect or prevent the emergence of a drug resistant phenotype.
PCT/US2003/016681 2002-05-28 2003-05-28 Functional protein expression for rapid cell-free phenotyping WO2003099229A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003243321A AU2003243321A1 (en) 2002-05-28 2003-05-28 Functional protein expression for rapid cell-free phenotyping

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US38352702P 2002-05-28 2002-05-28
US38363102P 2002-05-28 2002-05-28
US60/383,631 2002-05-28
US60/383,527 2002-05-28

Publications (2)

Publication Number Publication Date
WO2003099229A2 WO2003099229A2 (en) 2003-12-04
WO2003099229A3 true WO2003099229A3 (en) 2004-10-28

Family

ID=29587009

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/016681 WO2003099229A2 (en) 2002-05-28 2003-05-28 Functional protein expression for rapid cell-free phenotyping

Country Status (2)

Country Link
AU (1) AU2003243321A1 (en)
WO (1) WO2003099229A2 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6063562A (en) * 1994-09-16 2000-05-16 Sepracor, Inc. In vitro method for predicting the evolutionary response of HIV protease to a drug targeted thereagainst
US20010029022A1 (en) * 1999-08-25 2001-10-11 Christopher Fisher Methods of identifying anti-viral agents
US6537754B1 (en) * 1990-10-19 2003-03-25 Board Of Trustees Of The University Of Illinois Association of kinesin with sensitivity to chemotherapeutic drugs

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6537754B1 (en) * 1990-10-19 2003-03-25 Board Of Trustees Of The University Of Illinois Association of kinesin with sensitivity to chemotherapeutic drugs
US6063562A (en) * 1994-09-16 2000-05-16 Sepracor, Inc. In vitro method for predicting the evolutionary response of HIV protease to a drug targeted thereagainst
US20010029022A1 (en) * 1999-08-25 2001-10-11 Christopher Fisher Methods of identifying anti-viral agents

Also Published As

Publication number Publication date
WO2003099229A2 (en) 2003-12-04
AU2003243321A1 (en) 2003-12-12
AU2003243321A8 (en) 2003-12-12

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