WO2003099039A1 - Boisson contenant un agent fluorescent - Google Patents

Boisson contenant un agent fluorescent Download PDF

Info

Publication number
WO2003099039A1
WO2003099039A1 PCT/GB2003/002112 GB0302112W WO03099039A1 WO 2003099039 A1 WO2003099039 A1 WO 2003099039A1 GB 0302112 W GB0302112 W GB 0302112W WO 03099039 A1 WO03099039 A1 WO 03099039A1
Authority
WO
WIPO (PCT)
Prior art keywords
drink
phycoerythrin
fluorescent agent
phycocyanin
fluorescence
Prior art date
Application number
PCT/GB2003/002112
Other languages
English (en)
Inventor
Donald Keiller
Original Assignee
Anglia Polytechnic University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0211935A external-priority patent/GB0211935D0/en
Priority claimed from GB0228445A external-priority patent/GB0228445D0/en
Application filed by Anglia Polytechnic University filed Critical Anglia Polytechnic University
Priority to AU2003230031A priority Critical patent/AU2003230031A1/en
Publication of WO2003099039A1 publication Critical patent/WO2003099039A1/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12G—WINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
    • C12G3/00—Preparation of other alcoholic beverages
    • C12G3/04—Preparation of other alcoholic beverages by mixing, e.g. for preparation of liqueurs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52—Adding ingredients
    • A23L2/58—Colouring agents
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/40—Colouring or decolouring of foods
    • A23L5/42—Addition of dyes or pigments, e.g. in combination with optical brighteners
    • A23L5/46—Addition of dyes or pigments, e.g. in combination with optical brighteners using dyes or pigments of microbial or algal origin

Definitions

  • the present invention relates to a drink containing a fluorescent agent.
  • the present invention provides a drink containing a fluorescent agent.
  • the fluorescent agent can provide the drink with a striking visual appearance which may be appealing to consumers.
  • the fluorescent agent can be selected such that the drink illuminates under UV lighting of the type commonly used in bars and nightclubs.
  • Such establishments generally have low levels of ambient visible (white) light, and the fluorescent agent may be present in the drink in an amount such that fluorescence produced by the agent is visible unaided to the human eye under low levels of ambient visible light.
  • the fluorescent agent may be present in an amount such that fluorescence produced by the agent is detectable but any non-fluorescent colour produced by the agent in the drink is not visible to the human eye.
  • the fluorescent agent can serve as a "fingerprint" for the drink. The agent facilitates relatively easy authentication of the drink while remaining undetectable to the casual observer.
  • the fluorescent agent comprises one or more phycobiliproteins.
  • Phycobiliproteins are water soluble fluorescent proteins derived from cyanobacteria and eukaryotic algae. They are classified on the basis of their colour into two large groups, the phycoerythrins (red) and the phycocyanins (blue) . Absorption maxima for phycoerythrins typically lie in the range from 490 to 570 nm while absorption maxima for phycocyanins are typically found in the range from 610 to 665 nm. These large groups have been further subdivided to reflect variation among the proteins in determining the exact location of the absorbance maximum and the specific shape of the absorbance spectrum. For drink additive applications, phycobiliproteins have, in general, the advantage of being tasteless and odourless.
  • R-phycoerythrin was first isolated from the Rhodophyta .
  • the fluorescent agent comprises R- phycoerythrin, B-phycoerythrin, Y-phycoerythrin, C- phycocyanin, R-phycocyanin, allophycocyanin, allophycocyanin B, phycoerythrin 566, phycoerythrocyanin, phycourobilin, cryptoviolin, bilin 697, or a mixture of any two or more thereof.
  • these compounds, in particular C-phycocyanin and R- phycoerythrin are obtainable from organisms which are generally recognised as being safe for human consumption.
  • the fluorescent agent comprises C- phycocyanin and R-phycoerythrin or a mixture thereof.
  • the drink is an alcoholic drink e.g. containing at least 3% v/v (preferably at least 5, 10 or 20% v/v) of ethanol .
  • the drink may contain up to 50% v/v (preferably up to 40 or 30% v/v) of ethanol.
  • Conventional alcoholic drinks generally have a pH in the range 6-8, while conventional soft drinks can have a pH of up to 3. A reason for this difference is that alcoholic drinks rely to a greater or lesser extent on the alcohol to maintain sterility, whereas a low pH is often used to maintain the sterility of sugar-based soft drinks.
  • C-phycocyanin, R-phycoerythrin or the mixture thereof may be present in an amount according to the respective formula C > 20, R > 10, or C/2 + R > 10 (preferably C > 40, R > 20, or C/2 + R > 20), where C is the concentration of C-phycocyanin in the drink in ⁇ g/ml and R is the concentration of R-phycoerythrin in the drink in ⁇ g/ml.
  • the C- phycocyanin, R-phycoerythrin or the mixture thereof may be present in an amount according to the respective formula C ⁇ 0.5, R ⁇ 0.25, or C/2 + R ⁇ 0.25 (preferably C ⁇ 0.02, R ⁇ 0.01, or C/2 + R ⁇ 0.01).
  • the fluorescence can be detected at very low levels of fluorescent agent.
  • at least 0.001 ⁇ g/ml of the C-phycocyanin, R-phycoerythrin or the mixture thereof is present in the drink, in order to provide at least a minimum level of fluorescence such that the use of e.g. a photomultiplier to detect the fluorescence can be avoided.
  • the present invention provides for the use, in a drink, of a fluorescent agent comprising one or more phycobiliproteins.
  • a fluorescent agent comprising one or more phycobiliproteins.
  • Preferred and optional features of the drink and the phycobiliproteins are as described in the previous aspects of the invention.
  • Another aspect of the present invention provides a method for authentication of a drink, wherein the drink, if authentic, contains a fluorescent agent, the method comprising the steps of: (a) analysing the drink to detect fluorescence produced by the fluorescent agent;
  • step (b) determining the authenticity of the drink on the basis of whether fluorescence produced by the fluorescent agent is detected in step (a) .
  • the fluorescent agent may comprise one or more phycobiliproteins. Preferred and optional features of the drink and the phycobiliproteins are as described in the previous aspects of the invention.
  • C-phycocyanin used in the studies was extracted from Anaba ena cyl indrica and from commercially available Spirulina using standard biochemical extraction techniques (see e.g. Oi et al., Journal of Biology, 93, 981-986, (1982) ) .
  • C-phycocyanin extracted from Spirulina which has a long history of human consumption and is FDA approved, is preferred.
  • Rhodymenia pa lma ta also has a history of human consumption and is listed on the FDA' s GRAS (Generally Recognised As Safe) list as a food additive which is generally recognised as safe. Rhodymenia pa lma ta is also harvested and supplied commercially.
  • broad-band visible (white) light tungsten filament, or fluorescent tube
  • near-UV light 360-400nm
  • UVA 320-360nm
  • C-phycocyanin produced an intense red coloured fluorescence with an emission peak at 647 nm
  • R-phycoerythrin produced an intense yellow coloured fluorescence with an emission peak at 575 nm.
  • the fluorescence was, of course, most visible when the ambient visible light was at a low level.
  • the fluorescence was visible when the ambient visible (white) light was below 0.05 Wm "2 ( ⁇ 5 lux or - 0.1 ⁇ mols m ⁇ 2 s _1 ) in the presence of near UV light.
  • Wm "2 ⁇ 5 lux or - 0.1 ⁇ mols m ⁇ 2 s _1
  • Fluorescence produced by significantly lower concentrations (0.02 ⁇ g/ml for C-phycocyanin and 0.01 ⁇ g/ml for R-phycoerythrin) was still detectable when a suitable long-wave pass filter was placed between the observer and the UV-illuminated sample (which was otherwise in darkness) .
  • a suitable long-wave pass filter removes near UV or UVA light, thus allowing the faint fluorescence from C- phycocyanin and/or R-phycoerythrin present in the sample to be seen more easily.
  • a 550 nm long wave pass filter to be suitable for detecting R-phycoerythrin fluorescence and a 600 nm long wave pass filter to be suitable for detecting C-phycocyanin fluorescence.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Nutrition Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

L'invention concerne une boisson qui contient un agent fluorescent agent. En outre, cette boisson comprend une ou plusieurs phycobiliprotéines, comme de la R-phycoérythrine, B-phycoérythrine, Y-phycoérythrine, C-phycocyanine, R-phycocyanine, allophycocyanine, allophycocyanine B, phycoérythrine 566, phycoérythrocyanine, phycourobiline, cryptovioline et biline 697.
PCT/GB2003/002112 2002-05-23 2003-05-16 Boisson contenant un agent fluorescent WO2003099039A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003230031A AU2003230031A1 (en) 2002-05-23 2003-05-16 Drink containing fluorescent agent

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GB0211935A GB0211935D0 (en) 2002-05-23 2002-05-23 Drink containing fluorescent agent
GB0211935.2 2002-05-23
GB0228445A GB0228445D0 (en) 2002-12-05 2002-12-05 Drink containing fluorescent agent
GB0228445.3 2002-12-05

Publications (1)

Publication Number Publication Date
WO2003099039A1 true WO2003099039A1 (fr) 2003-12-04

Family

ID=29585819

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2003/002112 WO2003099039A1 (fr) 2002-05-23 2003-05-16 Boisson contenant un agent fluorescent

Country Status (2)

Country Link
AU (1) AU2003230031A1 (fr)
WO (1) WO2003099039A1 (fr)

Cited By (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007146328A2 (fr) * 2006-06-15 2007-12-21 Las Vegas Distilling Group Boisson fluorescente et méthode
FR2923990A1 (fr) * 2007-11-23 2009-05-29 Richard Virenque Boisson energisante fluorescente a base de cafeine.
EP2484230A1 (fr) 2011-02-03 2012-08-08 RUDOLF WILD GmbH & CO. KG Extrait de spiruline riche en protéines
US8989673B2 (en) 2012-09-28 2015-03-24 Medea Inc. System for delivering data to users
WO2015065887A1 (fr) * 2013-10-25 2015-05-07 Forelight, Llc Additifs pour boisson et boissons fermentées comprenant une protéine fluorescente
US9061797B2 (en) 2006-10-28 2015-06-23 Medea Inc. Bottle for alcoholic or non alcoholic beverages
US9152968B2 (en) 2007-06-22 2015-10-06 Medea Inc. System for and method of acting on beverage bottles
US9216844B2 (en) 2012-06-01 2015-12-22 Medea Inc. Container for beverages
US9376235B2 (en) 2009-11-02 2016-06-28 Medea Inc. Container for beverages
US9628434B2 (en) 2014-05-15 2017-04-18 Medea Inc. Programmable electronic devices on containers
US10009709B2 (en) 2015-03-26 2018-06-26 Medea Inc. Electronic device with network access via mobile device proxy
EP3375298A1 (fr) * 2017-03-17 2018-09-19 L'Abeille Composition liquide comprenant de la phycocyanine
ES2689974A1 (es) * 2017-05-15 2018-11-16 Sili Thinking S.L. Bebida alcohólica fluorescente y envase para dicha bebida
CN115052579A (zh) * 2020-01-31 2022-09-13 株式会社资生堂 透明质酸产生促进剂
CN116018072A (zh) * 2020-08-31 2023-04-25 嘉吉公司 用于肉代用品组合物的色素

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4851339A (en) * 1986-04-01 1989-07-25 Hills Christopher B Extraction of anti-mutagenic pigments from algae and vegetables
CN1127611A (zh) * 1995-12-28 1996-07-31 广东海洋生物食品有限公司 螺旋藻蓝色液体饮料及其制备方法
US6152358A (en) * 1996-02-06 2000-11-28 Bruce Bryan Bioluminescent novelty items

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4851339A (en) * 1986-04-01 1989-07-25 Hills Christopher B Extraction of anti-mutagenic pigments from algae and vegetables
CN1127611A (zh) * 1995-12-28 1996-07-31 广东海洋生物食品有限公司 螺旋藻蓝色液体饮料及其制备方法
US6152358A (en) * 1996-02-06 2000-11-28 Bruce Bryan Bioluminescent novelty items

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
DATABASE WPI Section Ch Week 199750, Derwent World Patents Index; Class D13, AN 1997-536589, XP002250818 *

Cited By (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007146328A2 (fr) * 2006-06-15 2007-12-21 Las Vegas Distilling Group Boisson fluorescente et méthode
WO2007146328A3 (fr) * 2006-06-15 2008-07-24 Las Vegas Distilling Group Boisson fluorescente et méthode
US9061797B2 (en) 2006-10-28 2015-06-23 Medea Inc. Bottle for alcoholic or non alcoholic beverages
US9836035B2 (en) 2007-06-22 2017-12-05 Medea Inc. System for and method of acting on containers
US9152968B2 (en) 2007-06-22 2015-10-06 Medea Inc. System for and method of acting on beverage bottles
FR2923990A1 (fr) * 2007-11-23 2009-05-29 Richard Virenque Boisson energisante fluorescente a base de cafeine.
EP2074893A1 (fr) 2007-11-23 2009-07-01 Richard Virenque Boisson énergisante fluorescente à base de caféine.
US9376235B2 (en) 2009-11-02 2016-06-28 Medea Inc. Container for beverages
EP2484230A1 (fr) 2011-02-03 2012-08-08 RUDOLF WILD GmbH & CO. KG Extrait de spiruline riche en protéines
WO2012104091A1 (fr) 2011-02-03 2012-08-09 Rudolf Wild Gmbh & Co. Kg Extraits de spiruline riches en protéines
US9216844B2 (en) 2012-06-01 2015-12-22 Medea Inc. Container for beverages
US10044915B2 (en) 2012-06-01 2018-08-07 Medea Inc. Container with camera and electronic display
US8989673B2 (en) 2012-09-28 2015-03-24 Medea Inc. System for delivering data to users
WO2015065887A1 (fr) * 2013-10-25 2015-05-07 Forelight, Llc Additifs pour boisson et boissons fermentées comprenant une protéine fluorescente
US9628434B2 (en) 2014-05-15 2017-04-18 Medea Inc. Programmable electronic devices on containers
US10121351B2 (en) 2014-05-15 2018-11-06 Medea Inc. Programmable electronic devices on containers
US10009709B2 (en) 2015-03-26 2018-06-26 Medea Inc. Electronic device with network access via mobile device proxy
US10433138B2 (en) 2015-03-26 2019-10-01 Medea Inc. Electronic device with network access via mobile device proxy
EP3375298A1 (fr) * 2017-03-17 2018-09-19 L'Abeille Composition liquide comprenant de la phycocyanine
FR3063875A1 (fr) * 2017-03-17 2018-09-21 L'abeille Composition liquide comprenant de la phycocyanine
ES2689974A1 (es) * 2017-05-15 2018-11-16 Sili Thinking S.L. Bebida alcohólica fluorescente y envase para dicha bebida
CN115052579A (zh) * 2020-01-31 2022-09-13 株式会社资生堂 透明质酸产生促进剂
CN116018072A (zh) * 2020-08-31 2023-04-25 嘉吉公司 用于肉代用品组合物的色素

Also Published As

Publication number Publication date
AU2003230031A1 (en) 2003-12-12

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