WO2003097796A3 - T7 bacteriophage display of fabs - Google Patents

T7 bacteriophage display of fabs Download PDF

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Publication number
WO2003097796A3
WO2003097796A3 PCT/US2003/014964 US0314964W WO03097796A3 WO 2003097796 A3 WO2003097796 A3 WO 2003097796A3 US 0314964 W US0314964 W US 0314964W WO 03097796 A3 WO03097796 A3 WO 03097796A3
Authority
WO
WIPO (PCT)
Prior art keywords
fabs
bacteriophage display
bacteriophage
display
disclosed
Prior art date
Application number
PCT/US2003/014964
Other languages
French (fr)
Other versions
WO2003097796A2 (en
Inventor
Russell P Rother
David R Geis
Original Assignee
Alexion Pharma Inc
Russell P Rother
David R Geis
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alexion Pharma Inc, Russell P Rother, David R Geis filed Critical Alexion Pharma Inc
Priority to AU2003229048A priority Critical patent/AU2003229048A1/en
Publication of WO2003097796A2 publication Critical patent/WO2003097796A2/en
Publication of WO2003097796A3 publication Critical patent/WO2003097796A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/12—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C07K16/1267—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-positive bacteria
    • C07K16/1282—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-positive bacteria from Clostridium (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/51—Complete heavy chain or Fd fragment, i.e. VH + CH1
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Control Of Indicators Other Than Cathode Ray Tubes (AREA)

Abstract

A T7 bacteriophage vector which displays Fab fragments is disclosed. Host cells and methods of making the bacteriophage are also described.
PCT/US2003/014964 2002-05-14 2003-05-13 T7 bacteriophage display of fabs WO2003097796A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003229048A AU2003229048A1 (en) 2002-05-14 2003-05-13 T7 bacteriophage display of fabs

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US38031802P 2002-05-14 2002-05-14
US60/380,318 2002-05-14

Publications (2)

Publication Number Publication Date
WO2003097796A2 WO2003097796A2 (en) 2003-11-27
WO2003097796A3 true WO2003097796A3 (en) 2004-06-03

Family

ID=29549952

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/014964 WO2003097796A2 (en) 2002-05-14 2003-05-13 T7 bacteriophage display of fabs

Country Status (2)

Country Link
AU (1) AU2003229048A1 (en)
WO (1) WO2003097796A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109439681A (en) * 2018-06-01 2019-03-08 沧州医学高等专科学校 A kind of recombinant plasmid can be used for showing a large amount of foreign proteins and construction method and application

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5766905A (en) * 1996-06-14 1998-06-16 Associated Universities Inc. Cytoplasmic bacteriophage display system
US6190908B1 (en) * 1998-08-12 2001-02-20 The Scripps Research Institute Modulation of polypeptide display on modified filamentous phage

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5766905A (en) * 1996-06-14 1998-06-16 Associated Universities Inc. Cytoplasmic bacteriophage display system
US6190908B1 (en) * 1998-08-12 2001-02-20 The Scripps Research Institute Modulation of polypeptide display on modified filamentous phage

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CARLOS F. BARBAS III ET AL.: "Assembly of combinatorial antibody libraries on phage surfaces: The gene III site", PNAS, vol. 88, no. 18, September 1991 (1991-09-01), pages 7978 - 7982, XP000652426 *
JANET L. CYR AND A.J. HUDSPETH: "A library of bacteriophage-displayed antibody fragments directed against proteins of the inner ear", PNAS, vol. 97, no. 5, 29 February 2000 (2000-02-29), pages 2276 - 2281, XP002975242 *
KANG A.S. ET AL.: "Linkage of recognition and replication functions by assembling combinatorial antibody Fab libraries along phage surfaces", PROC. NATL. ACAD. SCI., vol. 88, no. 10, May 1991 (1991-05-01), pages 4363 - 4366, XP000667378 *

Also Published As

Publication number Publication date
WO2003097796A2 (en) 2003-11-27
AU2003229048A1 (en) 2003-12-02
AU2003229048A8 (en) 2003-12-02

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