WO2003093471A8 - Novel 88 phage vectors - Google Patents

Novel 88 phage vectors

Info

Publication number
WO2003093471A8
WO2003093471A8 PCT/US2002/014971 US0214971W WO03093471A8 WO 2003093471 A8 WO2003093471 A8 WO 2003093471A8 US 0214971 W US0214971 W US 0214971W WO 03093471 A8 WO03093471 A8 WO 03093471A8
Authority
WO
WIPO (PCT)
Prior art keywords
phage
restriction site
engineered
novel
phage genome
Prior art date
Application number
PCT/US2002/014971
Other languages
French (fr)
Other versions
WO2003093471A1 (en
Inventor
Katherine S Bowdish
Shana Barbas-Frederickson
Original Assignee
Alexion Pharma Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alexion Pharma Inc filed Critical Alexion Pharma Inc
Priority to EP02744148A priority Critical patent/EP1444344A4/en
Priority to PCT/US2002/014971 priority patent/WO2003093471A1/en
Priority to CA002454837A priority patent/CA2454837A1/en
Priority to AU2002345537A priority patent/AU2002345537A1/en
Publication of WO2003093471A1 publication Critical patent/WO2003093471A1/en
Publication of WO2003093471A8 publication Critical patent/WO2003093471A8/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

A phage genome is engineered to include a novel restriction site at one of two different positions. In a first embodiment, a restriction site is inserted into the phage genome between the end of gene IV and the MOS hairpin which serves as a phage packaging signal for newly synthesized single strands of phage DNA. In a second embodiment, a restriction site is inserted into the phage genome after the MOS hairpin and prior to the minus strand origin. Once the phage genome is modified to contain the new restriction site, the vector can be engineered to be a '88' vector by inserting at the new restriction site a nucleotide sequence encoding at least a functional domain of pVIII and at least a first cloning site for receiving a gene encoding a polypeptide to be displayed and, optionally a second cloning site for receiving to be displayed. In particularly useful embodiments, the novel vectors are engineered to produce phage particles that display antibodies.
PCT/US2002/014971 2001-04-27 2002-04-29 Novel 88 phage vectors WO2003093471A1 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP02744148A EP1444344A4 (en) 2001-04-27 2002-04-29 Novel 88 phage vectors
PCT/US2002/014971 WO2003093471A1 (en) 2001-04-27 2002-04-29 Novel 88 phage vectors
CA002454837A CA2454837A1 (en) 2002-04-29 2002-04-29 Novel 88 phage vectors
AU2002345537A AU2002345537A1 (en) 2001-04-27 2002-04-30 Novel 88 phage vectors

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US28723901P 2001-04-27 2001-04-27
US60/287,239 2001-04-27
PCT/US2002/014971 WO2003093471A1 (en) 2001-04-27 2002-04-29 Novel 88 phage vectors

Publications (2)

Publication Number Publication Date
WO2003093471A1 WO2003093471A1 (en) 2003-11-13
WO2003093471A8 true WO2003093471A8 (en) 2004-07-22

Family

ID=32716530

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/014971 WO2003093471A1 (en) 2001-04-27 2002-04-29 Novel 88 phage vectors

Country Status (2)

Country Link
EP (1) EP1444344A4 (en)
WO (1) WO2003093471A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113999866B (en) * 2021-11-04 2023-09-05 南京农业大学 Phagemid vector for high-density display and application thereof

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU6235294A (en) * 1993-02-02 1994-08-29 Scripps Research Institute, The Methods for producing polypeptide binding sites
GB9313509D0 (en) * 1993-06-30 1993-08-11 Medical Res Council Chemisynthetic libraries
US5702892A (en) * 1995-05-09 1997-12-30 The United States Of America As Represented By The Department Of Health And Human Services Phage-display of immunoglobulin heavy chain libraries
WO2002088315A2 (en) * 2001-04-27 2002-11-07 Alexion Pharmaceuticals, Inc. Phagemid vectors

Also Published As

Publication number Publication date
WO2003093471A1 (en) 2003-11-13
EP1444344A4 (en) 2005-08-24
EP1444344A1 (en) 2004-08-11

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