WO2003089905A2 - Analyse informatisee de cellules anormales dans un bloc de cellules - Google Patents

Analyse informatisee de cellules anormales dans un bloc de cellules Download PDF

Info

Publication number
WO2003089905A2
WO2003089905A2 PCT/US2003/012534 US0312534W WO03089905A2 WO 2003089905 A2 WO2003089905 A2 WO 2003089905A2 US 0312534 W US0312534 W US 0312534W WO 03089905 A2 WO03089905 A2 WO 03089905A2
Authority
WO
WIPO (PCT)
Prior art keywords
cells
analysis
tissue according
cell
cell block
Prior art date
Application number
PCT/US2003/012534
Other languages
English (en)
Other versions
WO2003089905A3 (fr
Inventor
Stephen Frist
Richard S. F. Scott
Original Assignee
Cdx (Oralscan) Laboratories, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cdx (Oralscan) Laboratories, Inc. filed Critical Cdx (Oralscan) Laboratories, Inc.
Priority to EP03728489A priority Critical patent/EP1497644A4/fr
Priority to CA002483392A priority patent/CA2483392A1/fr
Priority to AU2003234180A priority patent/AU2003234180A1/en
Publication of WO2003089905A2 publication Critical patent/WO2003089905A2/fr
Publication of WO2003089905A3 publication Critical patent/WO2003089905A3/fr

Links

Classifications

    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
    • G01N1/06—Devices for withdrawing samples in the solid state, e.g. by cutting providing a thin slice, e.g. microtome
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/36—Embedding or analogous mounting of samples
    • G01N2001/368—Mounting multiple samples in one block, e.g. TMA [Tissue Microarrays]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N2015/1477—Multiparameters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated

Definitions

  • This invention relates to computer assisted cell! analysis, and more particularly computer assisted cell analysis for ceil blocks.
  • Computer assisted cell analysis is widely emplpyed, see references.
  • Automated cytolbgical specimen classification system andj method 5,257,182 Luck , et al. October 2J3, 1993. Morphological classification system and method: and, 4,965,725 Rutbnberg October 23, 1990, Neural network based automated cvtological specimeh classification system and method.
  • Cell blocks are formed by putting the cells ⁇ o a liquid, floating them in the liquid, apd spinning the liquid down in a centrifuge! to obtain a pellet of the cells. This pellet embedded in something similar to paraffjn so that the density of the paraffin and; the cells is similar, and one may cut this resultant hardened mass with a microtome and not shatter it.
  • the cell block preparation technique is employed in samples cohtaining numerous tissue fragments, for example fine needle aspiration samples (5,817,032 Williamson, IV, et al ) or brush bicpsy samples (6,297,044 Eisen, et al.,) which may be too small to be processe ih ⁇ stofogicalfy and too large to be viewed on a smear, cytospin or other liquid cytolog preparation
  • the primary difference between examining celliblock material as compared to material fr ⁇ fn smears or other cytologicaJ preparation ⁇ s that single cells or clusters of cells in the $mear are intact cells, whereas in a cell block, the cells are artificially forced together into a pellet and instead of looking at a cell on a slide, one-sees a cross-secti ⁇ nal slice through the cell. For example, if fine were to consider the nucleus to fc>e a sphere, when it is in its lying down condition, it will always have a certain diameter. When one slices the nucleus located in the cell block, the resultant diameter depends on the unknown position of the siic&. In contrast to histological preparation, of a specimen, where the large-scale tissue architecture is intact and can be examined in the sliced specimen, the tissue architecture visible in a cell block is limited to th tissue fragments present in the liquid.
  • One of the advantages of the present invention is [that computer assisted smear analysis provides a certain percentage accuracy in 'detecting suspect cells, whereas cell block analysis also has a certain percentage laccuracy as well. By combining both systems, the accuracy of detecting suspect celljs material increases. Of course, the preparation of the cell block for proper analysis enhances the effectiveness of the system. Although some of the computer analysis will bei looking at layered cells in the same mannier as previously encountered, the computer analysis will examine different morphological and color characteristics as related to cell block characteristics. Additionally, boundary characteristics! between cells will also be different in a cell block, and an examination of the boundaries between cells as indicating siiispect cells will also be examined.
  • planar cuts, the direction of the planes and the thickness of the cuts may all be easily! varied and will produce different characteristics for computer assisted analysis.

Abstract

La présente invention concerne un système d'analyse de blocs de cellules faisant appel à un système informatisé pour examiner un bloc de cellules découpé dans un amas de cellules, le bloc de cellules étant monté sur le système informatisé pour examiner une diversité de caractéristiques comprenant la couleur morphologique et les conditions aux limites entre les cellules. Le bloc de cellules peut être découpé de différentes façons pour présenter différentes caractéristiques à examiner par le système informatisé.
PCT/US2003/012534 2002-04-22 2003-04-22 Analyse informatisee de cellules anormales dans un bloc de cellules WO2003089905A2 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP03728489A EP1497644A4 (fr) 2002-04-22 2003-04-22 Analyse informatisee de cellules anormales dans un bloc de cellules
CA002483392A CA2483392A1 (fr) 2002-04-22 2003-04-22 Analyse informatisee de cellules anormales dans un bloc de cellules
AU2003234180A AU2003234180A1 (en) 2002-04-22 2003-04-22 Computerized analysis for abnormal cells in a cell block

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US37515002P 2002-04-22 2002-04-22
US60/375,150 2002-04-22

Publications (2)

Publication Number Publication Date
WO2003089905A2 true WO2003089905A2 (fr) 2003-10-30
WO2003089905A3 WO2003089905A3 (fr) 2004-02-12

Family

ID=29251235

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/012534 WO2003089905A2 (fr) 2002-04-22 2003-04-22 Analyse informatisee de cellules anormales dans un bloc de cellules

Country Status (4)

Country Link
EP (1) EP1497644A4 (fr)
AU (1) AU2003234180A1 (fr)
CA (1) CA2483392A1 (fr)
WO (1) WO2003089905A2 (fr)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5478722A (en) * 1991-02-17 1995-12-26 The Curators Of The University Of Missouri Preserved cell preparations for flow cytometry and immunology
US6300124B1 (en) * 1999-11-02 2001-10-09 Regents Of The University Of Minnesota Device and method to directly control the temperature of microscope slides

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5544650A (en) * 1988-04-08 1996-08-13 Neuromedical Systems, Inc. Automated specimen classification system and method
US5740270A (en) * 1988-04-08 1998-04-14 Neuromedical Systems, Inc. Automated cytological specimen classification system and method
GB8915759D0 (en) * 1989-07-10 1989-08-31 Shandon Scient Ltd Cell block preparation
DK2259042T3 (da) * 1998-10-05 2013-02-18 Biopath Automation Llc Fremgangsmåde til høstning og håndtering af vævsprøver til biopsianalyse

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5478722A (en) * 1991-02-17 1995-12-26 The Curators Of The University Of Missouri Preserved cell preparations for flow cytometry and immunology
US5776754A (en) * 1991-02-17 1998-07-07 The Curators Of The University Of Missouri Preserved cell preparations for flow cytometry and immunology
US6300124B1 (en) * 1999-11-02 2001-10-09 Regents Of The University Of Minnesota Device and method to directly control the temperature of microscope slides

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP1497644A2 *

Also Published As

Publication number Publication date
EP1497644A4 (fr) 2007-12-05
AU2003234180A8 (en) 2003-11-03
AU2003234180A1 (en) 2003-11-03
EP1497644A2 (fr) 2005-01-19
WO2003089905A3 (fr) 2004-02-12
CA2483392A1 (fr) 2003-10-30

Similar Documents

Publication Publication Date Title
Tannapfel et al. Prognostic value of ploidy and proliferation markers in renal cell carcinoma
Johnson et al. Flow cytometric analysis of head and neck carcinoma DNA index and S‐fraction from paraffin‐embedded sections: Comparison with malignancy grading
JP2002505432A (ja) 迅速な分子プロファイリングのための腫瘍組織マイクロアレイ
EP1891433B1 (fr) Procédé de classification d'échantillons cellulaires chimiquement réticulés par spectre de masse
EP1153300A2 (fr) Procedes et dispositifs d'isolation et d'analyse de la teneur proteique des cellules
EP1960757A1 (fr) Appareil et procedes d'evaluation automatisee d'une pathologie tissulaire
Hergt et al. Use of a 2‐tier histologic grading system for canine cutaneous mast cell tumors on cytology specimens
Huang et al. Comparison of nucleolar organiser regions and DNA flow cytometry in the evaluation of pleural effusion.
IL225125A (en) Diagnosis and evaluation of solid growth development by image processing
CN112534237A (zh) 分析前基底处理的系统和方法
US20110124530A1 (en) Pseudo-tissues and uses thereof
Bennington et al. DNA cytometry on four‐micrometer sections of paraffin‐embedded human renal adenocarcinomas and adenomas
CN109975090B (zh) 一种甲状腺、乳腺细针穿刺细胞组织块的制备方法
Connolly et al. Role of the Surgical Pathologist in the Diagnosis and Management of the Cancer Patient
CN1253716C (zh) 一种用于肿瘤早期诊断的组织芯片及制备器具
Sapierzynski Practical aspects of immunocytochemistry in canine lymphomas
WO2003089905A2 (fr) Analyse informatisee de cellules anormales dans un bloc de cellules
US20020127631A1 (en) Method for creating a frozen tissue array
Cör et al. Quantitative pathology of laryngeal epithelial hyperplastic lesions
US20020012938A1 (en) Detection of epithelial dysplasia
Murthy et al. Comparative Study of Conventional Smears, Cytospin Smears and Cell Block on Body Fluid Cytology
Tanabe et al. Touch imprint cytology is useful for the intraoperative pathological diagnosis of PitNETs
Melega et al. Cell blocks in veterinary medicine: A comparison of two methods (cell tube and agar) in 52 effusions from dogs and cats
Srilatha et al. Comparative analysis of staining efficacy of Leishman's stain with hematoxylin and eosin stain in the assessment of keratin pearl formation and mitotic figures in different grades of oral squamous cell carcinoma
Millward 1001 useful things you can investigate with a microscope.

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ OM PH PL PT RO RU SD SE SG SK SL TJ TM TN TR TT TZ UA UG UZ VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 2483392

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 2003728489

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2003728489

Country of ref document: EP

NENP Non-entry into the national phase

Ref country code: JP

WWW Wipo information: withdrawn in national office

Country of ref document: JP