WO2003089641A3 - Dual condensation/epimerization domain in non-ribosomal peptide synthetase systems - Google Patents

Dual condensation/epimerization domain in non-ribosomal peptide synthetase systems Download PDF

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Publication number
WO2003089641A3
WO2003089641A3 PCT/CA2003/000575 CA0300575W WO03089641A3 WO 2003089641 A3 WO2003089641 A3 WO 2003089641A3 CA 0300575 W CA0300575 W CA 0300575W WO 03089641 A3 WO03089641 A3 WO 03089641A3
Authority
WO
WIPO (PCT)
Prior art keywords
epimerization
dual condensation
ribosomal peptide
peptide synthetase
condensation
Prior art date
Application number
PCT/CA2003/000575
Other languages
French (fr)
Other versions
WO2003089641A2 (en
Inventor
Chris M Farnet
Alfredo Staffa
Original Assignee
Ecopia Biosciences Inc
Chris M Farnet
Alfredo Staffa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ecopia Biosciences Inc, Chris M Farnet, Alfredo Staffa filed Critical Ecopia Biosciences Inc
Priority to AU2003222690A priority Critical patent/AU2003222690A1/en
Priority to CA002430580A priority patent/CA2430580A1/en
Publication of WO2003089641A2 publication Critical patent/WO2003089641A2/en
Publication of WO2003089641A3 publication Critical patent/WO2003089641A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
    • C07K1/047—Simultaneous synthesis of different peptide species; Peptide libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention relates to domains of non-ribosomal peptide synthetases (NRPSs) that exhibit dual condensation and epimerization activities. The 'dual condensation/epimerization NRPS domains' of the present invention allow for the incorporation of non-proteinogenic substrates, such as D-amino acids, into peptide products. These dual condensation/epimerization NRPS domains may further be used to modify the stereochemistry of synthesized peptides at selected amino acid sites.
PCT/CA2003/000575 2002-04-17 2003-04-17 Dual condensation/epimerization domain in non-ribosomal peptide synthetase systems WO2003089641A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2003222690A AU2003222690A1 (en) 2002-04-17 2003-04-17 Dual condensation/epimerization domain in non-ribosomal peptide synthetase systems
CA002430580A CA2430580A1 (en) 2002-04-17 2003-04-17 Specialized dual condensation/epimerization domain in non-ribosomal peptide synthetase systems

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US37279002P 2002-04-17 2002-04-17
US60/372,790 2002-04-17

Publications (2)

Publication Number Publication Date
WO2003089641A2 WO2003089641A2 (en) 2003-10-30
WO2003089641A3 true WO2003089641A3 (en) 2004-06-10

Family

ID=27805336

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA2003/000575 WO2003089641A2 (en) 2002-04-17 2003-04-17 Dual condensation/epimerization domain in non-ribosomal peptide synthetase systems

Country Status (3)

Country Link
AU (1) AU2003222690A1 (en)
CA (1) CA2430580A1 (en)
WO (1) WO2003089641A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2002212014A1 (en) * 2000-10-13 2002-04-22 Ecopia Biosciences Inc. Gene cluster for ramoplanin biosynthesis
CN114657155B (en) * 2021-12-23 2024-01-19 中国农业科学院生物技术研究所 Novel non-ribosomal polypeptide synthetase resolution expression method
CN117384924B (en) * 2023-12-11 2024-06-25 中国农业科学院生物技术研究所 Polypeptide biosynthesis method based on enzyme domain resolution

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4303646A (en) * 1979-04-07 1981-12-01 Gruppo Lepetit S.P.A. Antibiotic A/16686 and process for preparation thereof

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4303646A (en) * 1979-04-07 1981-12-01 Gruppo Lepetit S.P.A. Antibiotic A/16686 and process for preparation thereof

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
CHIU HSIEN-TAI ET AL: "Molecular cloning and sequence analysis of the complestatin biosynthetic gene cluster.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 98, no. 15, 17 July 2001 (2001-07-17), July 17, 2001, pages 8548 - 8553, XP002253211, ISSN: 0027-8424 *
DATABASE SWISSPROT [online] "Syringopeptin synthetase", XP002253210, accession no. SWALL Database accession no. Q9FDB3 *
DOEKEL S ET AL: "DIPEPTIDE FORMATION ON ENGINEERED HYBRID PEPTIDE SYNTHETASES", CHEMISTRY AND BIOLOGY, CURRENT BIOLOGY, LONDON, GB, vol. 7, no. 6, June 2000 (2000-06-01), pages 373 - 384, XP000911752, ISSN: 1074-5521 *
GUENZI E ET AL: "Characterization of the syringomycin synthetase gene cluster", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, US, vol. 273, no. 49, 4 December 1998 (1998-12-04), pages 32857 - 32863, XP002158169, ISSN: 0021-9258 *
MARAHIEL M A ET AL: "Modular peptide synthetases involved in nonribosomal peptide synthesis", CHEMICAL REVIEWS, AMERICAN CHEMICAL SOCIETY. EASTON, US, vol. 97, no. 7, November 1997 (1997-11-01), pages 2651 - 2673, XP002133489, ISSN: 0009-2665 *
SCHAUWECKER F ET AL: "CONSTRUCTION AND IN VITRO ANALYSIS OF A NEW BI MODULAR POLYPEPTIDE SYNTHETASE FOR SYNTHESIS OF N-METHYLATED ACYL PEPTIDES", CHEMISTRY AND BIOLOGY, CURRENT BIOLOGY, LONDON, GB, vol. 7, no. 4, April 2000 (2000-04-01), pages 287 - 297, XP000957461, ISSN: 1074-5521 *
SCHOLZ-SCHROEDER BRENDA K ET AL: "The contribution of syringopeptin and syringomycin to virulence of Pseudomonas syringae pv. syringae strain B301D on the basis of sypA and syrB1 biosynthesis mutant analysis.", MOLECULAR PLANT-MICROBE INTERACTIONS, vol. 14, no. 3, March 2001 (2001-03-01), pages 336 - 348, XP009016624 *

Also Published As

Publication number Publication date
AU2003222690A1 (en) 2003-11-03
CA2430580A1 (en) 2003-09-04
WO2003089641A2 (en) 2003-10-30

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