WO2003085094A3 - Solid phase methods for polynucleotide production - Google Patents
Solid phase methods for polynucleotide production Download PDFInfo
- Publication number
- WO2003085094A3 WO2003085094A3 PCT/US2003/009814 US0309814W WO03085094A3 WO 2003085094 A3 WO2003085094 A3 WO 2003085094A3 US 0309814 W US0309814 W US 0309814W WO 03085094 A3 WO03085094 A3 WO 03085094A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- oligonucleotide
- solid support
- target polynucleotide
- solid phase
- annealed
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
Abstract
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2003582273A JP2005521419A (en) | 2002-04-01 | 2003-04-01 | Solid phase method for polynucleotide production |
CA002478983A CA2478983A1 (en) | 2002-04-01 | 2003-04-01 | Solid phase methods for polynucleotide production |
EP03714474A EP1499626A2 (en) | 2002-04-01 | 2003-04-01 | Solid phase methods for polynucleotide production |
AU2003218470A AU2003218470A1 (en) | 2002-04-01 | 2003-04-01 | Solid phase methods for polynucleotide production |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US36947802P | 2002-04-01 | 2002-04-01 | |
US60/369,478 | 2002-04-01 | ||
US39052202P | 2002-06-20 | 2002-06-20 | |
US60/390,522 | 2002-06-20 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2003085094A2 WO2003085094A2 (en) | 2003-10-16 |
WO2003085094A3 true WO2003085094A3 (en) | 2004-11-25 |
Family
ID=28794364
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2003/009814 WO2003085094A2 (en) | 2002-04-01 | 2003-04-01 | Solid phase methods for polynucleotide production |
Country Status (6)
Country | Link |
---|---|
US (1) | US20030228602A1 (en) |
EP (1) | EP1499626A2 (en) |
JP (1) | JP2005521419A (en) |
AU (1) | AU2003218470A1 (en) |
CA (1) | CA2478983A1 (en) |
WO (1) | WO2003085094A2 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US11845054B2 (en) | 2010-11-12 | 2023-12-19 | Gen9, Inc. | Methods and devices for nucleic acids synthesis |
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US7563600B2 (en) | 2002-09-12 | 2009-07-21 | Combimatrix Corporation | Microarray synthesis and assembly of gene-length polynucleotides |
US7879580B2 (en) * | 2002-12-10 | 2011-02-01 | Massachusetts Institute Of Technology | Methods for high fidelity production of long nucleic acid molecules |
US7932025B2 (en) * | 2002-12-10 | 2011-04-26 | Massachusetts Institute Of Technology | Methods for high fidelity production of long nucleic acid molecules with error control |
JP2007508825A (en) * | 2003-10-15 | 2007-04-12 | ブルー ヘロン バイオテクノロジー インコーポレイテッド | Solid phase method for polynucleotide production |
CA2590245A1 (en) * | 2004-11-11 | 2006-05-18 | Modular Genetics, Inc. | Ladder assembly and system for generating diversity |
WO2007070726A2 (en) * | 2005-12-16 | 2007-06-21 | Gene Oracle, Inc. | A gene synthesis kit |
WO2008027558A2 (en) | 2006-08-31 | 2008-03-06 | Codon Devices, Inc. | Iterative nucleic acid assembly using activation of vector-encoded traits |
WO2009138954A2 (en) * | 2008-05-14 | 2009-11-19 | British Columbia Cancer Agency Branch | Gene synthesis by convergent assembly of oligonucleotide subsets |
US8999679B2 (en) | 2008-12-18 | 2015-04-07 | Iti Scotland Limited | Method for assembly of polynucleic acid sequences |
GB2481425A (en) | 2010-06-23 | 2011-12-28 | Iti Scotland Ltd | Method and device for assembling polynucleic acid sequences |
WO2012064975A1 (en) | 2010-11-12 | 2012-05-18 | Gen9, Inc. | Protein arrays and methods of using and making the same |
DE102010056289A1 (en) | 2010-12-24 | 2012-06-28 | Geneart Ag | Process for the preparation of reading frame correct fragment libraries |
EP2944693B1 (en) | 2011-08-26 | 2019-04-24 | Gen9, Inc. | Compositions and methods for high fidelity assembly of nucleic acids |
US9150853B2 (en) | 2012-03-21 | 2015-10-06 | Gen9, Inc. | Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis |
EP4001427A1 (en) | 2012-04-24 | 2022-05-25 | Gen9, Inc. | Methods for sorting nucleic acids and multiplexed preparative in vitro cloning |
JP6509727B2 (en) | 2012-06-25 | 2019-05-15 | ギンゴー バイオワークス, インコーポレイテッド | Methods for nucleic acid assembly and high-throughput sequencing |
TWI721929B (en) | 2013-08-05 | 2021-03-11 | 美商扭轉生物科技有限公司 | De novo synthesized gene libraries |
AU2014363967A1 (en) * | 2013-12-09 | 2017-01-05 | Shawn Allen | Long nucleic acid sequences containing variable regions |
CA2965485A1 (en) * | 2014-10-21 | 2016-04-28 | Martin R. Schiller | Methods and compositions for screening molecular function comprising chimeric minimotifs |
WO2016126987A1 (en) | 2015-02-04 | 2016-08-11 | Twist Bioscience Corporation | Compositions and methods for synthetic gene assembly |
CA2975852A1 (en) | 2015-02-04 | 2016-08-11 | Twist Bioscience Corporation | Methods and devices for de novo oligonucleic acid assembly |
WO2016172377A1 (en) | 2015-04-21 | 2016-10-27 | Twist Bioscience Corporation | Devices and methods for oligonucleic acid library synthesis |
US10407460B2 (en) | 2015-05-07 | 2019-09-10 | The Regents Of The University Of Califonia | Solid phase sequence-independent nucleic acid assembly |
WO2017015348A1 (en) * | 2015-07-20 | 2017-01-26 | The Regents Of The University Of California | Sequence-independent nucleic acid assembly |
IL258164B (en) | 2015-09-18 | 2022-09-01 | Twist Bioscience Corp | Methods for modulating protein and cellular activity and method for nucleic acid synthesis |
WO2017053450A1 (en) | 2015-09-22 | 2017-03-30 | Twist Bioscience Corporation | Flexible substrates for nucleic acid synthesis |
CN108603307A (en) | 2015-12-01 | 2018-09-28 | 特韦斯特生物科学公司 | functionalized surface and its preparation |
GB201612011D0 (en) | 2016-07-11 | 2016-08-24 | Glaxosmithkline Ip Dev Ltd | Novel processes for the production of oligonucleotides |
WO2018038772A1 (en) | 2016-08-22 | 2018-03-01 | Twist Bioscience Corporation | De novo synthesized nucleic acid libraries |
JP6871364B2 (en) | 2016-09-21 | 2021-05-12 | ツイスト バイオサイエンス コーポレーション | Nucleic acid-based data storage |
EA201991262A1 (en) | 2016-12-16 | 2020-04-07 | Твист Байосайенс Корпорейшн | LIBRARIES OF OPTIONS OF IMMUNOLOGICAL SYNAPSIS AND THEIR SYNTHESIS |
SG11201907713WA (en) | 2017-02-22 | 2019-09-27 | Twist Bioscience Corp | Nucleic acid based data storage |
EP3595674A4 (en) | 2017-03-15 | 2020-12-16 | Twist Bioscience Corporation | Variant libraries of the immunological synapse and synthesis thereof |
WO2018231864A1 (en) | 2017-06-12 | 2018-12-20 | Twist Bioscience Corporation | Methods for seamless nucleic acid assembly |
CN111566209A (en) | 2017-06-12 | 2020-08-21 | 特韦斯特生物科学公司 | Seamless nucleic acid assembly method |
US11407837B2 (en) | 2017-09-11 | 2022-08-09 | Twist Bioscience Corporation | GPCR binding proteins and synthesis thereof |
GB2582865B (en) * | 2017-09-11 | 2022-05-04 | Synthego Corp | Biopolymer synthesis system and method |
KR20240024357A (en) | 2017-10-20 | 2024-02-23 | 트위스트 바이오사이언스 코포레이션 | Heated nanowells for polynucleotide synthesis |
CN111542605A (en) * | 2017-12-27 | 2020-08-14 | 东丽株式会社 | Method for recovering nucleic acid |
US10936953B2 (en) | 2018-01-04 | 2021-03-02 | Twist Bioscience Corporation | DNA-based digital information storage with sidewall electrodes |
KR20210013128A (en) | 2018-05-18 | 2021-02-03 | 트위스트 바이오사이언스 코포레이션 | Polynucleotides, reagents and methods for nucleic acid hybridization |
AU2020229349A1 (en) | 2019-02-26 | 2021-10-14 | Twist Bioscience Corporation | Variant nucleic acid libraries for GLP1 receptor |
SG11202109283UA (en) | 2019-02-26 | 2021-09-29 | Twist Bioscience Corp | Variant nucleic acid libraries for antibody optimization |
EP3987019A4 (en) | 2019-06-21 | 2023-04-19 | Twist Bioscience Corporation | Barcode-based nucleic acid sequence assembly |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5942609A (en) * | 1998-11-12 | 1999-08-24 | The Porkin-Elmer Corporation | Ligation assembly and detection of polynucleotides on solid-support |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5437979A (en) * | 1989-07-24 | 1995-08-01 | Beckman Instruments, Inc. | Solid phase system for sequential reactions |
US5705344A (en) * | 1996-03-14 | 1998-01-06 | Tularik, Inc. | High-throughput screening assay for inhibitors of nucleic acid helicases |
US6277622B1 (en) * | 1997-08-11 | 2001-08-21 | The University Of Sydney | Synthetic polynucleotides |
US6670127B2 (en) * | 1997-09-16 | 2003-12-30 | Egea Biosciences, Inc. | Method for assembly of a polynucleotide encoding a target polypeptide |
-
2003
- 2003-04-01 AU AU2003218470A patent/AU2003218470A1/en not_active Abandoned
- 2003-04-01 EP EP03714474A patent/EP1499626A2/en not_active Withdrawn
- 2003-04-01 JP JP2003582273A patent/JP2005521419A/en active Pending
- 2003-04-01 US US10/405,907 patent/US20030228602A1/en not_active Abandoned
- 2003-04-01 WO PCT/US2003/009814 patent/WO2003085094A2/en active Search and Examination
- 2003-04-01 CA CA002478983A patent/CA2478983A1/en not_active Abandoned
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5942609A (en) * | 1998-11-12 | 1999-08-24 | The Porkin-Elmer Corporation | Ligation assembly and detection of polynucleotides on solid-support |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US11845054B2 (en) | 2010-11-12 | 2023-12-19 | Gen9, Inc. | Methods and devices for nucleic acids synthesis |
Also Published As
Publication number | Publication date |
---|---|
AU2003218470A1 (en) | 2003-10-20 |
WO2003085094A2 (en) | 2003-10-16 |
EP1499626A2 (en) | 2005-01-26 |
CA2478983A1 (en) | 2003-10-16 |
US20030228602A1 (en) | 2003-12-11 |
JP2005521419A (en) | 2005-07-21 |
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